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Information on EC 3.1.3.12 - trehalose-phosphatase and Organism(s) Thermoplasma acidophilum and UniProt Accession Q9HIW7

for references in articles please use BRENDA:EC3.1.3.12
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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.3 Phosphoric-monoester hydrolases
                3.1.3.12 trehalose-phosphatase
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This record set is specific for:
Thermoplasma acidophilum
UNIPROT: Q9HIW7 not found.
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The taxonomic range for the selected organisms is: Thermoplasma acidophilum
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
trehalose-6-phosphate phosphatase, attps1, ostpp1, gob-1, trehalose 6-phosphate phosphatase, otsb2, trehalose-6-phosphate synthase/phosphatase, trehalose phosphate phosphatase, trehalose-phosphate phosphatase, t6p phosphatase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TPP
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trehalose 6-phosphatase
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-
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trehalose 6-phosphate phosphatase
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trehalose phosphate phosphatase
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-
-
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trehalose-6-phosphate phosphatase-related protein
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-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
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SYSTEMATIC NAME
IUBMB Comments
alpha,alpha-trehalose-6-phosphate phosphohydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
9025-72-3
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
alpha,alpha-trehalose 6-phosphate + H2O
alpha,alpha-trehalose + phosphate
show the reaction diagram
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-
-
?
trehalose 6-phosphate + H2O
trehalose + phosphate
show the reaction diagram
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-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
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dependent
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.7
trehalose 6-phosphate
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in 50 mM Tris-HCl (pH 8.0), 5 mM dithiothreitol, and 20 mM MgCl2, at 50°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
10
trehalose 6-phosphate
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in 50 mM Tris-HCl (pH 8.0), 5 mM dithiothreitol, and 20 mM MgCl2, at 50°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
sitting drop vapor diffusion crystallization of both the native and SeMet proteins
sitting drop vapour diffusion method with 30% PEG 4000, 0.2 M MgCl2, and 0.1 M Tris-HCl (pH 8.5)
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA resin column chromatography and S200 gel filtration
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Rao, K.N.; Kumaran, D.; Seetharaman, J.; Bonanno, J.B.; Burley, S.K.; Swaminathan, S.
Crystal structure of trehalose-6-phosphate phosphatase-related protein: biochemical and biological implications
Protein Sci.
15
1735-1744
2006
Thermoplasma acidophilum, Thermoplasma acidophilum TA1209
Manually annotated by BRENDA team
Rao, K.; Kumaran, D.; Seetharaman, J.; Bonanno, J.; Burley, S.; Swaminathan, S.
Crystal structure of trehalose-6-phosphate phosphatase-related protein Biochemical and biological implications
Protein Sci.
15
1735-1744
2006
Thermoplasma acidophilum (Q9HIW7)
Manually annotated by BRENDA team