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IUBMB Comments The enzyme can catalyse the sequential hydrolysis of tri-, di-, and mono-phosphorylated oxetanocin A compounds, releasing one molecule of inorganic phosphate at a time. The active site switches from a dinuclear to a mononuclear metal center as phosphates are eliminated from the substrate.
Reaction Schemes
4'-phosphooxetanocin A
+
=
oxetanocin A
+
Synonyms oxsA ,
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oxsA
-
-
-
-
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4'-phosphooxetanocin A + H2O = oxetanocin A + phosphate
-
-
-
-
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[(2S,3R,4R)-4-(6-aminopurin-9-yl)-3-(hydroxymethyl)oxetan-2-yl]methyl phosphate phosphohydrolase
The enzyme can catalyse the sequential hydrolysis of tri-, di-, and mono-phosphorylated oxetanocin A compounds, releasing one molecule of inorganic phosphate at a time. The active site switches from a dinuclear to a mononuclear metal center as phosphates are eliminated from the substrate.
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dADP + 2 H2O
2'-deoxyadenosine + 2 phosphate
dAMP + H2O
2'-deoxyadenosine + phosphate
dATP + 3 H2O
2'-deoxyadenosine + 3 phosphate
oxetanocin A 5'-phosphate + H2O
oxetanocin A + phosphate
oxetanocin A diphosphate + H2O
oxetanocin A + 2 phosphate
oxetanocin A triphosphate + H2O
oxetanocin A + 3 phosphate
Substrates: -
?
dADP + 2 H2O
2'-deoxyadenosine + 2 phosphate
Substrates: -
?
dADP + 2 H2O
2'-deoxyadenosine + 2 phosphate
Substrates: -
?
dAMP + H2O
2'-deoxyadenosine + phosphate
Substrates: -
?
dAMP + H2O
2'-deoxyadenosine + phosphate
Substrates: -
?
dATP + 3 H2O
2'-deoxyadenosine + 3 phosphate
Substrates: -
?
dATP + 3 H2O
2'-deoxyadenosine + 3 phosphate
Substrates: -
?
oxetanocin A 5'-phosphate + H2O
oxetanocin A + phosphate
Substrates: -
?
oxetanocin A 5'-phosphate + H2O
oxetanocin A + phosphate
Substrates: -
?
oxetanocin A diphosphate + H2O
oxetanocin A + 2 phosphate
Substrates: -
?
oxetanocin A diphosphate + H2O
oxetanocin A + 2 phosphate
Substrates: -
?
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Co2+
the enzyme shows higher in vitro activity with 0.6 mM Co2+ than Mg2+
Mg2+
contains Mg2+, shows low activity in the presence of 0.6 mM Mg2+
Mn2+
the enzyme shows low activity with 0.6 mM Mn2+
Zn2+
the enzyme shows lowest activity with 0.6 mM Zn2+
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0.053
dADP
at pH 8.0 and 37°C
0.022
dAMP
at pH 8.0 and 37°C
0.155
dATP
at pH 8.0 and 37°C
0.011
oxetanocin A 5'-phosphate
at pH 8.0 and 37°C
-
0.013
oxetanocin A diphosphate
at pH 8.0 and 37°C
-
0.008
oxetanocin A triphosphate
at pH 8.0 and 37°C
-
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0.0026
dADP
at pH 8.0 and 37°C
0.0037
dAMP
at pH 8.0 and 37°C
0.022
dATP
at pH 8.0 and 37°C
0.0012
oxetanocin A 5'-phosphate
at pH 8.0 and 37°C
-
0.0029
oxetanocin A diphosphate
at pH 8.0 and 37°C
-
0.0045
oxetanocin A triphosphate
at pH 8.0 and 37°C
-
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0.051
dADP
at pH 8.0 and 37°C
0.167
dAMP
at pH 8.0 and 37°C
0.142
dATP
at pH 8.0 and 37°C
0.106
oxetanocin A 5'-phosphate
at pH 8.0 and 37°C
-
0.221
oxetanocin A diphosphate
at pH 8.0 and 37°C
-
0.525
oxetanocin A triphosphate
at pH 8.0 and 37°C
-
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-
UniProt
brenda
-
UniProt
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Highest Expressing Human Cell Lines
Filter by:
Cell Line Links
Gene Links
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metabolism
the enzyme is involved in the biosynthesis of the antitumor, antiviral, and antibacterial compound oxetanocin A
metabolism
-
the enzyme is involved in the biosynthesis of the antitumor, antiviral, and antibacterial compound oxetanocin A
-
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OXSA_PRIMG
194
0
22680
Swiss-Prot
-
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homodimer
-
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free enzyme and enzyme bound to oxetanocin A-5'-monophosphate, hanging drop vapor diffusion method, using 0.1 M Tris hydrochloride (pH 8.5), 0.2 M magnesium chloride hexahydrate, 15% (w/v) PEG 4000
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ammonium sulfate precipitation and DEAE-Sepharose column chromatography
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expressed in Escherichia coli BL21-star-(DE3) cells
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Bridwell-Rabb, J.; Kang, G.; Zhong, A.; Liu, H.; Drennan, C.
An HD domain phosphohydrolase active site tailored for oxetanocin-A biosynthesis
Proc. Natl. Acad. Sci. USA
113
13750-13755
2016
Priestia megaterium (O24769), Priestia megaterium NK84-0128 (O24769)
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