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IUBMB Comments The enzyme, characterized from Pseudomonas species, participates in a peptidoglycan salvage pathway.
The enzyme appears in viruses and cellular organisms
Synonyms mupP , MurNAc 6P phosphatase, N-acetylmuramic acid 6-phosphate phosphatase, more
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N-acetylmuramic acid 6-phosphate phosphatase
mupP
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N-acetylmuramic acid 6-phosphate phosphatase
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N-acetylmuramic acid 6-phosphate phosphatase
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N-acetyl-D-muramate 6-phosphate + H2O = N-acetyl-D-muramate + phosphate
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MetaCyc
peptidoglycan recycling II
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N-acetyl-D-muramate 6-phosphate phosphohydrolase
The enzyme, characterized from Pseudomonas species, participates in a peptidoglycan salvage pathway.
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N-acetyl-D-glucosamine 6-phosphate + H2O
N-acetyl-D-glucosamine + phosphate
Substrates: - Products: -
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N-acetyl-D-muramate 6-phosphate + H2O
N-acetyl-D-muramate + phosphate
additional information
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N-acetyl-D-muramate 6-phosphate + H2O
N-acetyl-D-muramate + phosphate
Substrates: - Products: -
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N-acetyl-D-muramate 6-phosphate + H2O
N-acetyl-D-muramate + phosphate
Substrates: - Products: -
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additional information
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Substrates: no substrates: N-acetyl-D-muramate 1-phosphate, N-acetyl-D-glucosamine 6-phosphate, N-acetyl-D-glucosamine 1-phosphate, N-acetyl-D-glucosamine, D-glucose, D-glucosamine 6-phosphate, glucose 6-phosphate Products: -
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additional information
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Substrates: neither alpha-1-phosphorylated N-acetyl-D-muramate, N-acetyl-D-glucose, or D-glucose nor glucosamine 6-phosphate or D-glucose 6-phosphate is used as a substrate Products: -
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additional information
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Substrates: no substrates: N-acetyl-D-muramate 1-phosphate, N-acetyl-D-glucosamine 6-phosphate, N-acetyl-D-glucosamine 1-phosphate, N-acetyl-D-glucosamine, D-glucose, D-glucosamine 6-phosphate, glucose 6-phosphate Products: -
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N-acetyl-D-muramate 6-phosphate + H2O
N-acetyl-D-muramate + phosphate
Substrates: - Products: -
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Mg2+
very low activity in absence of Mg2+, 16fold increase in activity in presence of 10 mM Mg2+
Mg2+
10 mM used in assay conditions
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0.31
N-acetyl-D-muramate 6-phosphate
0.31
N-acetyl-D-muramate 6-phosphate
pH 7.6, 22°C
0.31
N-acetyl-D-muramate 6-phosphate
at pH 7.6 and 22°C
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0.88
N-acetyl-D-muramate 6-phosphate
0.88
N-acetyl-D-muramate 6-phosphate
pH 7.6, 22°C
0.88
N-acetyl-D-muramate 6-phosphate
at pH 7.6 and 22°C
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2.84
N-acetyl-D-muramate 6-phosphate
2.84
N-acetyl-D-muramate 6-phosphate
pH 7.6, 22°C
2.84
N-acetyl-D-muramate 6-phosphate
at pH 7.6 and 22°C
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4 - 10
at pH 4.0 and pH 10.0, the enzyme activity is 40 and 60%, respectively, of the maximum
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UniProt
brenda
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UniProt
brenda
Highest Expressing Human Cell Lines
Filter by:
Cell Line Links
Gene Links
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physiological function
enzyme is part of the anabolic recycling pathway of Pseudomonas species and specifically converts MurNAc 6-phosphate to MurNAc. A MupP mutant is highly susceptible to fosfomycin, accumulates large amounts of MurNAc 6-phosphate, and shows lower levels of UDP-MurNAc than wild-type cells
physiological function
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enzyme is part of the anabolic recycling pathway of Pseudomonas species and specifically converts MurNAc 6-phosphate to MurNAc. A MupP mutant is highly susceptible to fosfomycin, accumulates large amounts of MurNAc 6-phosphate, and shows lower levels of UDP-MurNAc than wild-type cells
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MUPP_PSEAE
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
226
0
24977
Swiss-Prot
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MUPP_PSEPK
Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)
223
0
24373
Swiss-Prot
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A0A6J5DWS4_9BURK
238
0
24938
TrEMBL
-
A0A6S6ZG41_9BURK
224
0
24256
TrEMBL
-
A0A6J5FN93_9BURK
237
0
25370
TrEMBL
-
A0A653X6Z5_9PSED
227
0
24806
TrEMBL
-
A0A6S7FEI5_9BURK
224
0
24157
TrEMBL
-
A0A6S7DFI6_9BURK
224
0
24211
TrEMBL
-
A0A6J5FZD4_9BURK
243
0
25693
TrEMBL
-
A0A6S7CQD5_9BURK
208
0
23175
TrEMBL
-
B1G2X5_PARG4
Paraburkholderia graminis (strain ATCC 700544 / DSM 17151 / LMG 18924 / NCIMB 13744 / C4D1M)
237
0
25534
TrEMBL
-
A0A6S7AZM7_9BURK
224
0
24289
TrEMBL
-
A0A654BA71_9PSED
222
0
24625
TrEMBL
-
A0A6M4H8P4_9PROT
225
0
24071
TrEMBL
-
A0A7R7AI89_9GAMM
Methyloprofundus sp
225
0
24601
TrEMBL
-
A0A8H8XT90_9PSED
223
0
24926
TrEMBL
-
A0A346BEI7_9BURK
237
0
25557
TrEMBL
-
A0A6S6ZLG4_9BURK
224
0
24374
TrEMBL
-
A0A7U7ERE2_9PSED
223
0
24510
TrEMBL
-
A0A2N7WU82_9BURK
237
0
25644
TrEMBL
-
A0A6S7EFK6_9BURK
224
0
24265
TrEMBL
-
A0A5Q4YTZ8_9BURK
Paraburkholderia dioscoreae
237
0
25548
TrEMBL
-
A0A653YFC4_9PSED
223
0
24310
TrEMBL
-
A0A1X1PHM1_9BURK
238
0
25099
TrEMBL
-
A0A811GUQ4_9BURK
224
0
24420
TrEMBL
-
A0A6J5B0P3_9BURK
237
0
25556
TrEMBL
-
A0A6S7F7B6_9BURK
208
0
22967
TrEMBL
-
A0A6J5EK17_9BURK
237
0
25607
TrEMBL
-
A0A653HSZ8_9GAMM
236
0
26200
TrEMBL
-
A0A6S6XZT1_9PROT
222
0
24102
TrEMBL
-
A0A6S7CIE7_9BURK
232
0
24490
TrEMBL
-
A0A653X4J3_9PSED
223
0
24504
TrEMBL
-
A0A7T7BCT4_RALPI
229
0
23890
TrEMBL
-
A0A6S6Z330_9BURK
224
0
24149
TrEMBL
-
A0A653JAT7_9BURK
224
0
23947
TrEMBL
-
A0A6S7CS58_9BURK
224
0
24216
TrEMBL
-
A0A811GPT0_9BURK
208
0
23209
TrEMBL
-
A0A654A4A2_9PSED
223
0
24475
TrEMBL
-
A0A6J5ATM1_9BURK
237
0
25610
TrEMBL
-
A0A6S7CJJ2_9BURK
242
0
25335
TrEMBL
-
A0A6S7BN88_9BURK
237
0
25443
TrEMBL
-
A0A653U5U0_9PSED
223
0
24611
TrEMBL
-
A0A6S7CGT6_9BURK
208
0
23138
TrEMBL
-
A0A6S7DH57_9BURK
224
0
24255
TrEMBL
-
A0A6J5AJ14_9BURK
224
0
24232
TrEMBL
-
A0A6S6ZBF0_9BURK
208
0
23211
TrEMBL
-
A0A6J5K5U3_9BURK
237
0
25654
TrEMBL
-
A0A653XM57_9BURK
254
0
27446
TrEMBL
-
A0A653P574_9BURK
235
0
24993
TrEMBL
-
A0A6J5EF95_9BURK
237
0
25599
TrEMBL
-
A0A6S7C3D1_9BURK
224
0
24229
TrEMBL
-
A0A6G8NKP2_9BURK
260
0
28432
TrEMBL
-
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x * 25890, calculated, recombinant His-tagged protein
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x * 25890, calculated from amino acid sequence
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x * 25890, calculated, recombinant His-tagged protein
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22
40 min, 10% loss of activity
22 - 45
the recombinant enzyme loses about 10% of its activity at 22°C and 70% at 37°C within 40 min and is completely inactivated within 40 min at 45°C. About 50% of its activity remain at 22°C, and about 85% of its activity remain at 37°C
37
40 min, 70% loss of activity
45
40 min, complete inactivation
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the long-term stability of the enzyme in solution at -80°C is increased by the addition of 20% (v/v) glycerol
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Ni-NTA column chromatography and Superdex 200 gel filtration
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expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
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Borisova, M.; Gisin, J.; Mayer, C.
The N-acetylmuramic acid 6-phosphate phosphatase MupP completes the Pseudomonas peptidoglycan recycling pathway leading to intrinsic fosfomycin resistance
mBio
8
e00092
2017
Pseudomonas putida (Q88M11), Pseudomonas putida DSM 6125 (Q88M11)
brenda
Unsleber, S.; Borisova, M.; Mayer, C.
Enzymatic synthesis and semi-preparative isolation of N-acetylmuramic acid 6-phosphate
Carbohydr. Res.
445
98-103
2017
Pseudomonas putida (Q88M11)
brenda
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