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(E)-N'-(1-(3-(4-fluorophenyl)-5-phenyl-4,5-dihydro-1H-pyrazol-1-yl)ethylidene)isonicotinohydrazide
-
(E)-N'-(4'-chlorobenzylidene)isonicotinohydrazide
-
(E)-N'-(4-hydroxy-3-methoxybenzylidene)isonicotinohydrazide
most potent inhibitor for tissue-non-specific alkaline phosphatase
1-[[3-(2,4-dichlorophenyl)-1H-pyrazol-5-yl]carbonyl]-4-ethylpiperazine
-
1-[[3-(2,4-dichlorophenyl)-1H-pyrazol-5-yl]carbonyl]-4-methylpiperazine
-
1-[[3-(2,4-dichlorophenyl)-1H-pyrazol-5-yl]carbonyl]azepane
-
2-(4-[[3-(2,4-dichlorophenyl)-1H-pyrazol-5-yl]carbonyl]piperazin-1-yl)ethanol
-
3-(2,4-dichloro-5-fluorophenyl)-N,N-bis(2-hydroxyethyl)-1H-pyrazole-5-carboxamide
-
3-(2,4-dichloro-5-fluorophenyl)-N,N-dimethyl-1H-pyrazole-5-carboxamide
-
3-(2,4-dichloro-5-fluorophenyl)-N-(1-methylethyl)-1H-pyrazole-5-carboxamide
-
3-(2,4-dichloro-5-fluorophenyl)-N-(2-hydroxyethyl)-1H-pyrazole-5-carboxamide
-
3-(2,4-dichloro-5-fluorophenyl)-N-(3-hydroxypropyl)-1H-pyrazole-5-carboxamide
-
3-(2,4-dichlorophenyl)-1H-pyrazole-5-carbohydrazide
-
3-(2,4-dichlorophenyl)-N,N-bis(2-hydroxyethyl)-1H-pyrazole-5-carboxamide
-
3-(2,4-dichlorophenyl)-N,N-diethyl-1H-pyrazole-5-carboxamide
-
3-(2,4-dichlorophenyl)-N,N-dimethyl-1H-pyrazole-5-carboxamide
-
3-(2,4-dichlorophenyl)-N-(1-methylethyl)-1H-pyrazole-5-carboxamide
-
3-(2,4-dichlorophenyl)-N-(2-hydroxyethyl)-1H-pyrazole-5-carboxamide
-
3-(2,4-dichlorophenyl)-N-(2-methoxyethyl)-1H-pyrazole-5-carboxamide
-
3-(2,4-dichlorophenyl)-N-(2-methylpropyl)-1H-pyrazole-5-carboxamide
-
3-(2,4-dichlorophenyl)-N-(3-hydroxypropyl)-1H-pyrazole-5-carboxamide
-
3-(2,4-dichlorophenyl)-N-(3-methylbutyl)-1H-pyrazole-5-carboxamide
-
3-(2,4-dichlorophenyl)-N-(4-hydroxybutyl)-1H-pyrazole-5-carboxamide
-
3-(2,4-difluorophenyl)-N-(2-hydroxyethyl)-1H-pyrazole-5-carboxamide
-
4-[[3-(2,4-dichlorophenyl)-1H-pyrazol-5-yl]carbonyl]morpholine
-
N'-[(Z)-(2-hydroxyphenyl)methylidene]pyridine-4-carbohydrazide
-
N'-[(Z)-(3-nitrophenyl)methylidene]pyridine-4-carbohydrazide
-
N'-[(Z)-(4-bromophenyl)methylidene]pyridine-4-carbohydrazide
-
N'-[(Z)-(4-fluorophenyl)methylidene]pyridine-4-carbohydrazide
-
N'-[(Z)-(4-methoxyphenyl)methylidene]pyridine-4-carbohydrazide
-
N'-[(Z)-(pyridin-3-yl)methylidene]pyridine-4-carbohydrazide
-
N'-[(Z)-(pyridin-4-yl)methylidene]pyridine-4-carbohydrazide
-
N-(2-hydroxyethyl)-3-(2,3,4-trichlorophenyl)-1H-pyrazole-5-carboxamide
-
N-(2-hydroxyethyl)-3-(2,4,5-trifluorophenyl)-1H-pyrazole-5-carboxamide
-
N-tert-butyl-3-(2,4-dichlorophenyl)-1H-pyrazole-5-carboxamide
-
(2Z)-2-(benzoylamino)-3-[2-[4-(trifluoromethyl)phenyl]-1-benzothiophen-3-yl]prop-2-enoate
-
with 0.1 mM 35% remaining activity
(4-methoxyphenyl)[2-(4-methoxyphenyl)-1-benzothiophen-3-yl]methanone
-
with 0.1 mM 70% remaining activity
(E)-N'-(1-(3-(4-fluorophenyl)-5-phenyl-4,5-dihydro-1H-pyrazol-1-yl)ethylidene)isonicotinohydrazide
-
(E)-N'-(4'-chlorobenzylidene)isonicotinohydrazide
most potent inhibitor for intestinal alkaline phosphatase
(E)-N'-(4-hydroxy-3-methoxybenzylidene)isonicotinohydrazide
-
1,10-phenanthroline
-
1 mM, irreversibly inactivates the enzyme
1-benzothiophen-3-yl(4-methoxyphenyl)methanone
-
with 0.1 mM 40% remaining activity
1-benzothiophen-3-yl(phenyl)methanone
-
with 0.1 mM 43% remaining activity
2,4-dichloro-N-((4-(N-(pyrimidin-2-yl)sulfamoyl)phenyl)carbamothioyl)benzamide
-
-
2-(2-chlorophenyl)-1-benzothiophene-3-carbaldehyde
-
with 0.1 mM 63% remaining activity
2-(2-methylphenyl)-1-benzothiophene-3-carbonitrile
-
with 0.1 mM 5% remaining activity
2-(3-chlorophenyl)-1-benzothiophene-3-carbaldehyde
-
with 0.1 mM 62% remaining activity
2-(3-methoxy-1-benzothiophen-2-yl)benzonitrile
-
with 0.1 mM 57% remaining activity
2-(4-chlorophenyl)-1-benzothiophene-3-carbaldehyde
-
with 0.1 mM 9% remaining activity
2-(4-chlorophenyl)-1-benzothiophene-3-carbonitrile
-
with 0.1 mM 69% remaining activity
2-(4-methoxyphenyl)-1-benzothiophene-3-carbaldehyde
-
with 0.1 mM 37% remaining activity
2-(naphthalen-2-yl)-1-benzothiophene-3-carbonitrile
-
with 0.1 mM 18% remaining activity
2-chloro-N-((4-(N-(pyrimidin-2-yl)sulfamoyl)phenyl)carbamothioyl)benzamide
-
-
2-iodo-1-benzothiophene
-
with 0.1 mM 41% remaining activity
2-phenyl-1-benzothiophene-3-carbaldehyde
-
with 0.1 mM 41% remaining activity
2-phenyl-1-benzothiophene-3-carbonitrile
-
with 0.1 mM 76% remaining activity
2-phenyl-3-oxime-benzo[b]thiophene
-
with 0.1 mM 1% remaining activity
2-[3-(2,2,2-trifluoroethyl)-1-benzothiophen-2-yl]benzonitrile
-
with 0.1 mM 70% remaining activity
2-[3-(4-fluorophenoxy)-1-benzothiophen-2-yl]benzonitrile
-
with 0.1 mM 66% remaining activity
2-[4-(1-benzothiophen-3-yl)piperazin-1-yl]phenol
-
with 0.1 mM 49% remaining activity
2-[4-(trifluoromethyl)phenyl]-1-benzothiophene-3-carbaldehyde
-
with 0.1 mM 30% remaining activity
3,5-dinitro-N-((4-(N-(pyrimidin-2-yl)sulfamoyl)phenyl)carbamothioyl)benzamide
-
-
3-(3-methoxy-1-benzothiophen-2-yl)quinoline
-
with 0.1 mM 64% remaining activity
3-methoxy-2-(2-methylphenyl)-1-benzothiophene
-
with 0.1 mM 73% remaining activity
3-methoxy-2-(4-methoxyphenyl)-1-benzothiophene
-
with 0.1 mM 78% remaining activity
4-(1-benzothiophen-3-yloxy)benzaldehyde
-
with 0.1 mM 9% remaining activity
4-chloro-N-((4-(N-(pyrimidin-2-yl)sulfamoyl)phenyl)carbamothioyl)benzamide
-
-
4-fluoro-N-((4-(N-(pyrimidin-2-yl)sulfamoyl)phenyl)carbamothioyl)benzamide
-
-
4-methoxy-N-((4-(N-(pyrimidin-2-yl)sulfamoyl)phenyl)carbamothioyl)benzamide
-
-
arsenate
-
irreversible inhibitor
bis(1,1,1-trifluoroacetylacetonato)oxovanadium(IV)
-
-
bis(3-chloroacetylacetonato)oxovanadium(IV)
-
-
bis(3-chloroacetylacetonato)oxovanadium(IV)(4-Mepy)
-
-
bis(3-methylacetylacetonato)oxovanadium(IV)
-
-
bis(acetylacetonato)oxovanadium(IV)
-
-
bis(acetylacetonato)oxovanadium(IV)(4-Mepy)
-
-
bis(benzoylacetonato)oxovanadium(IV)
-
-
butanol
-
reversible noncompetitive
CO2
-
enzyme in buffer can be inactivated by COS treatment at atmospheric pressure, increasing inactivation, when temperature increases from 20°C to 50°C, 16% loss of activity after 30 min at 20°C, 81% loss of activity after 30 min at 50°C. Change in activity dependent on CO2 treatment is not observed in raw milk mainly due to strong buffering capacity of milk
ethanol
-
reversible noncompetitive
ethylene glycol
-
reversible noncompetitive
KCl
-
reduced enzymatic activity and significantly decreased Vmax/Km ratio
KNO3
-
reduced enzymatic activity and significantly decreased Vmax/Km ratio
KPF6
-
reduced enzymatic activity and significantly decreased Vmax/Km ratio
methyl (2E)-2-(benzoylamino)-3-[2-(4-methoxyphenyl)-1-benzothiophen-3-yl]prop-2-enoate
-
with 0.1 mM 77% remaining activity
methyl N-benzoyl-3-[2-[4-(trifluoromethyl)phenyl]-1-benzothiophen-3-yl]alaninate
-
with 0.1 mM 63% remaining activity
N'-[(Z)-(2-hydroxyphenyl)methylidene]pyridine-4-carbohydrazide
-
N'-[(Z)-(3-nitrophenyl)methylidene]pyridine-4-carbohydrazide
-
N'-[(Z)-(4-bromophenyl)methylidene]pyridine-4-carbohydrazide
-
N'-[(Z)-(4-fluorophenyl)methylidene]pyridine-4-carbohydrazide
-
N'-[(Z)-(4-methoxyphenyl)methylidene]pyridine-4-carbohydrazide
-
N'-[(Z)-(pyridin-3-yl)methylidene]pyridine-4-carbohydrazide
-
N'-[(Z)-(pyridin-4-yl)methylidene]pyridine-4-carbohydrazide
-
N-((4-(N-(pyrimidin-2-yl)sulfamoyl)phenyl)carbamothioyl)acetamide
-
-
N-((4-(N-(pyrimidin-2-yl)sulfamoyl)phenyl)carbamothioyl)butyramide
-
-
N-((4-(N-(pyrimidin-2-yl)sulfamoyl)phenyl)carbamothioyl)heptanamide
-
-
N-((4-(N-(pyrimidin-2-yl)sulfamoyl)phenyl)carbamothioyl)octanamide
-
-
Na2SO4
-
reduced enzymatic activity and significantly decreased Vmax/Km ratio
NaBF4
-
reduced enzymatic activity and significantly decreased Vmax/Km ratio
NaCl
-
reduces enzymatic activity and significantly decreased Vmax/Km ratio
NaNO3
-
reduced enzymatic activity and significantly decreased Vmax/Km ratio
phosphate
-
competitive inhibitor
Propanol
-
reversible noncompetitive
Sodium acetate
-
reduced enzymatic activity and significantly decreased Vmax/Km ratio
Sodium citrate
-
reduced enzymatic activity and significantly decreased Vmax/Km ratio
levamisole
-
-
theophylline
-
theophylline
-
reversible non-competitive inhibitor
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0.037 - 0.1159
3-(4-methoxyspiro [1,2-dioxetane-3,2'-(5'-chloro)-tricyclo [3.3.1.13,7]-decan]-4-yl)phenyl phosphate
0.00000035 - 7.32
4-nitrophenyl phosphate
0.62
p-nitrophenyl phosphate
-
-
additional information
4-nitrophenyl phosphate
-
the Km values increase with increasing concentrations of diethanolamine and N-methylethanolamine, but not triethanolamine
0.037
3-(4-methoxyspiro [1,2-dioxetane-3,2'-(5'-chloro)-tricyclo [3.3.1.13,7]-decan]-4-yl)phenyl phosphate
layer-by-layer single-stack, immobilized ALP
0.04
3-(4-methoxyspiro [1,2-dioxetane-3,2'-(5'-chloro)-tricyclo [3.3.1.13,7]-decan]-4-yl)phenyl phosphate
direct physical adsorption, immobilized ALP
0.04
3-(4-methoxyspiro [1,2-dioxetane-3,2'-(5'-chloro)-tricyclo [3.3.1.13,7]-decan]-4-yl)phenyl phosphate
immobilized ALP
0.05
3-(4-methoxyspiro [1,2-dioxetane-3,2'-(5'-chloro)-tricyclo [3.3.1.13,7]-decan]-4-yl)phenyl phosphate
free ALP
0.1058
3-(4-methoxyspiro [1,2-dioxetane-3,2'-(5'-chloro)-tricyclo [3.3.1.13,7]-decan]-4-yl)phenyl phosphate
layer-by-layer multi-stack, immobilized ALP
0.114
3-(4-methoxyspiro [1,2-dioxetane-3,2'-(5'-chloro)-tricyclo [3.3.1.13,7]-decan]-4-yl)phenyl phosphate
glutaraldehyde X-link DAH, immobilized ALP
0.1159
3-(4-methoxyspiro [1,2-dioxetane-3,2'-(5'-chloro)-tricyclo [3.3.1.13,7]-decan]-4-yl)phenyl phosphate
glutaraldehyde X-link, immobilized ALP
0.00000035
4-nitrophenyl phosphate
-
-
0.07
4-nitrophenyl phosphate
-
pH 9.8, 20°C, 0.05 M diethanolamine
0.082
4-nitrophenyl phosphate
-
pH 9.5, 37°C, Vmax: 0.96 nmol/min/mg enzyme, CIAP immobilized enzyme reactor (in excellent agreement with a Km value of calf ALP enzyme using a 96-well microplate reader)
0.09
4-nitrophenyl phosphate
-
pH 9.8, 20°C, 0.05 M methylamine
0.11
4-nitrophenyl phosphate
-
37°C, native enzyme
0.17
4-nitrophenyl phosphate
-
57°C, native enzyme
0.18
4-nitrophenyl phosphate
-
57°C, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride
0.18
4-nitrophenyl phosphate
-
pH 9.8, 20°C, 0.2 M N-methylethanolamine
0.2
4-nitrophenyl phosphate
-
37°C, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride
0.23
4-nitrophenyl phosphate
-
37°C, native enzyme, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride
0.24
4-nitrophenyl phosphate
-
pH 9.8, 20°C, 0.1 M triethanolamine
0.3
4-nitrophenyl phosphate
-
pH 9.8, 20°C, 0.05 M ethanolamine
0.3
4-nitrophenyl phosphate
-
pH 9.8, 20°C, 1 M ethanolamine
0.31
4-nitrophenyl phosphate
-
pH 9.8, 20°C, 1 M triethanolamine
0.35
4-nitrophenyl phosphate
-
pH 9.8, 20°C, 0.02 M dimethylamine
0.35
4-nitrophenyl phosphate
-
pH 9.8, 20°C, 0.02 M ethylamine
0.4
4-nitrophenyl phosphate
-
37°C, pH 9.5, glycine-NaOH buffer
0.43
4-nitrophenyl phosphate
-
pH 9.8, 20°C, 1 M ethylamine
0.62
4-nitrophenyl phosphate
-
pH 9.8, 20°C, 1 M methylamine
0.65
4-nitrophenyl phosphate
-
pH 9.8, 20°C, 1 M dimethylamine
0.69
4-nitrophenyl phosphate
-
pH 10, 37°C, Vmax: 0.02 mM/min
0.76
4-nitrophenyl phosphate
-
37°C, pH 11, Tris-HCl buffer
0.83
4-nitrophenyl phosphate
-
pH 9.8, 20°C, 1 M diethanolamine
0.833
4-nitrophenyl phosphate
-
at pH 9.5 and 37°C
1.37
4-nitrophenyl phosphate
-
pH not specified, 37°C, Vmax: 0.011 mM/min, in the presence of 0.8 M NaN3
1.91
4-nitrophenyl phosphate
-
pH 9.5, 37°C, Vmax: 0.029 mM/min, in the presence of 0.8 M KNO3
2.48
4-nitrophenyl phosphate
-
40% sucrose, -4.5°C, frozen
2.5 - 3
4-nitrophenyl phosphate
-
pH 9.8, 20°C, 1 M N-methylethanolamine
2.52
4-nitrophenyl phosphate
-
pH 9.5, 37°C, Vmax: 0.023 mM/min, in the presence of 0.8 M KCl
2.54
4-nitrophenyl phosphate
-
pH 10, 37°C, Vmax: 0.018 mM/min, in the presence of 0.8 M NaAc
2.73
4-nitrophenyl phosphate
-
pH 9.25, 37°C, Vmax: 0.02 mM/min, in the presence of 0.8 M KPF6
2.97
4-nitrophenyl phosphate
-
40% sucrose, -4.5°C, unfrozen
3.43
4-nitrophenyl phosphate
-
pH 9.25, 37°C, Vmax: 0.021 mM/min, in the presence of 0.8 M NaNO3
4.4
4-nitrophenyl phosphate
-
pH 9, 37°C, Vmax: 0.016 mM/min, in the presence of 0.8 M NaBF4
4.42
4-nitrophenyl phosphate
-
pH 9.5, 37°C, Vmax: 0.028 mM/min, in the presence of 0.8 M NaCl
6.4
4-nitrophenyl phosphate
-
40% sucrose, 25°C
6.87
4-nitrophenyl phosphate
-
pH 9, 37°C, Vmax: 0.022 mM/min, in the presence of 0.8 M Na2SO4
7.32
4-nitrophenyl phosphate
-
pH not specified, 37°C, Vmax: 0.019 mM/min, in the presence of 0.8 M Na citrate
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0.037 - 2250
4-nitrophenyl phosphate
additional information
additional information
-
-
-
0.037 - 0.23
4-nitrophenyl phosphate
-
37°C, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride
20 - 50
4-nitrophenyl phosphate
-
57°C, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride
42.55
4-nitrophenyl phosphate
-
37°C, pH 9.5, glycine-NaOH buffer
65
4-nitrophenyl phosphate
-
37°C, native enzyme
77.4
4-nitrophenyl phosphate
-
37°C, native enzyme, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride
78.83
4-nitrophenyl phosphate
-
37°C, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride
82.98
4-nitrophenyl phosphate
-
37°C, pH 11, Tris-HCl buffer
87.83
4-nitrophenyl phosphate
-
57°C, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride
115.9
4-nitrophenyl phosphate
-
37°C, native enzyme
140
4-nitrophenyl phosphate
-
pH 9.8, 20°C, 0.05 M methylamine
150
4-nitrophenyl phosphate
-
pH 9.8, 20°C, 0.02 M dimethylamine
183.3
4-nitrophenyl phosphate
-
57°C, native enzyme
190
4-nitrophenyl phosphate
-
pH 9.8, 20°C, 0.05 M diethanolamine
300
4-nitrophenyl phosphate
-
pH 9.8, 20°C, 1 M ethanolamine
330
4-nitrophenyl phosphate
-
pH 9.8, 20°C, 1 M methylamine
370
4-nitrophenyl phosphate
-
pH 9.8, 20°C, 1 M dimethylamine
400
4-nitrophenyl phosphate
-
pH 9.8, 20°C, 1 M ethylamine
450
4-nitrophenyl phosphate
-
pH 9.8, 20°C, 0.02 M ethylamine
500
4-nitrophenyl phosphate
-
pH 9.8, 20°C, 0.2 M N-methylethanolamine
610
4-nitrophenyl phosphate
-
pH 9.8, 20°C, 0.05 M ethanolamine
840
4-nitrophenyl phosphate
-
pH 9.8, 20°C, 0.1 M triethanolamine
1240
4-nitrophenyl phosphate
-
pH 9.8, 20°C, 1 M diethanolamine
1450
4-nitrophenyl phosphate
-
pH 9.8, 20°C, 1 M triethanolamine
2250
4-nitrophenyl phosphate
-
pH 9.8, 20°C, 1 M N-methylethanolamine
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0.00106
(E)-N'-(1-(3-(4-fluorophenyl)-5-phenyl-4,5-dihydro-1H-pyrazol-1-yl)ethylidene)isonicotinohydrazide
Bos taurus
tissue-non-specific alkaline phosphatase, pH and temperature not specified in the publication
0.00035
(E)-N'-(4-hydroxy-3-methoxybenzylidene)isonicotinohydrazide
Bos taurus
tissue-non-specific alkaline phosphatase, pH and temperature not specified in the publication
0.000488
1-[[3-(2,4-dichlorophenyl)-1H-pyrazol-5-yl]carbonyl]-4-ethylpiperazine
Bos taurus
-
0.000828
1-[[3-(2,4-dichlorophenyl)-1H-pyrazol-5-yl]carbonyl]-4-methylpiperazine
Bos taurus
-
0.00145
1-[[3-(2,4-dichlorophenyl)-1H-pyrazol-5-yl]carbonyl]azepane
Bos taurus
-
0.000375
2-(4-[[3-(2,4-dichlorophenyl)-1H-pyrazol-5-yl]carbonyl]piperazin-1-yl)ethanol
Bos taurus
-
0.000127
3-(2,4-dichloro-5-fluorophenyl)-N,N-bis(2-hydroxyethyl)-1H-pyrazole-5-carboxamide
Bos taurus
-
0.000324
3-(2,4-dichloro-5-fluorophenyl)-N,N-dimethyl-1H-pyrazole-5-carboxamide
Bos taurus
-
0.00485
3-(2,4-dichloro-5-fluorophenyl)-N-(1-methylethyl)-1H-pyrazole-5-carboxamide
Bos taurus
-
0.0001
3-(2,4-dichloro-5-fluorophenyl)-N-(2-hydroxyethyl)-1H-pyrazole-5-carboxamide
Bos taurus
-
0.000056
3-(2,4-dichloro-5-fluorophenyl)-N-(3-hydroxypropyl)-1H-pyrazole-5-carboxamide
Bos taurus
-
0.000044
3-(2,4-dichlorophenyl)-1H-pyrazole-5-carbohydrazide
Bos taurus
-
0.000047
3-(2,4-dichlorophenyl)-N,N-bis(2-hydroxyethyl)-1H-pyrazole-5-carboxamide
Bos taurus
-
0.000285
3-(2,4-dichlorophenyl)-N,N-diethyl-1H-pyrazole-5-carboxamide
Bos taurus
-
0.000119
3-(2,4-dichlorophenyl)-N,N-dimethyl-1H-pyrazole-5-carboxamide
Bos taurus
-
0.000051
3-(2,4-dichlorophenyl)-N-(1-methylethyl)-1H-pyrazole-5-carboxamide
Bos taurus
-
0.000044
3-(2,4-dichlorophenyl)-N-(2-hydroxyethyl)-1H-pyrazole-5-carboxamide
Bos taurus
-
0.000046
3-(2,4-dichlorophenyl)-N-(2-methoxyethyl)-1H-pyrazole-5-carboxamide
Bos taurus
-
0.000082
3-(2,4-dichlorophenyl)-N-(2-methylpropyl)-1H-pyrazole-5-carboxamide
Bos taurus
-
0.000031
3-(2,4-dichlorophenyl)-N-(3-hydroxypropyl)-1H-pyrazole-5-carboxamide
Bos taurus
-
0.000459
3-(2,4-dichlorophenyl)-N-(3-methylbutyl)-1H-pyrazole-5-carboxamide
Bos taurus
-
0.000035
3-(2,4-dichlorophenyl)-N-(4-hydroxybutyl)-1H-pyrazole-5-carboxamide
Bos taurus
-
0.000035
3-(2,4-difluorophenyl)-N-(2-hydroxyethyl)-1H-pyrazole-5-carboxamide
Bos taurus
-
0.000574
4-[[3-(2,4-dichlorophenyl)-1H-pyrazol-5-yl]carbonyl]morpholine
Bos taurus
-
0.0192
levamisole
Bos taurus
tissue-non-specific alkaline phosphatase, pH and temperature not specified in the publication
0.00182
N'-[(Z)-(3-nitrophenyl)methylidene]pyridine-4-carbohydrazide
Bos taurus
tissue-non-specific alkaline phosphatase, pH and temperature not specified in the publication
0.00413
N'-[(Z)-(4-bromophenyl)methylidene]pyridine-4-carbohydrazide
Bos taurus
tissue-non-specific alkaline phosphatase, pH and temperature not specified in the publication
0.00226
N'-[(Z)-(4-fluorophenyl)methylidene]pyridine-4-carbohydrazide
Bos taurus
tissue-non-specific alkaline phosphatase, pH and temperature not specified in the publication
0.00056
N'-[(Z)-(4-methoxyphenyl)methylidene]pyridine-4-carbohydrazide
Bos taurus
tissue-non-specific alkaline phosphatase, pH and temperature not specified in the publication
0.00243
N'-[(Z)-(pyridin-3-yl)methylidene]pyridine-4-carbohydrazide
Bos taurus
tissue-non-specific alkaline phosphatase, pH and temperature not specified in the publication
0.000005
N-(2-hydroxyethyl)-3-(2,3,4-trichlorophenyl)-1H-pyrazole-5-carboxamide
Bos taurus
-
0.000134
N-(2-hydroxyethyl)-3-(2,4,5-trifluorophenyl)-1H-pyrazole-5-carboxamide
Bos taurus
-
0.000398
N-tert-butyl-3-(2,4-dichlorophenyl)-1H-pyrazole-5-carboxamide
Bos taurus
-
0.00398
(E)-N'-(1-(3-(4-fluorophenyl)-5-phenyl-4,5-dihydro-1H-pyrazol-1-yl)ethylidene)isonicotinohydrazide
Bos taurus
intestinal alkaline phosphatase, pH and temperature not specified in the publication
0.00067
(E)-N'-(4'-chlorobenzylidene)isonicotinohydrazide
Bos taurus
intestinal alkaline phosphatase, pH and temperature not specified in the publication
0.00192
(E)-N'-(4-hydroxy-3-methoxybenzylidene)isonicotinohydrazide
Bos taurus
intestinal alkaline phosphatase, pH and temperature not specified in the publication
0.000344
2,4-dichloro-N-((4-(N-(pyrimidin-2-yl)sulfamoyl)phenyl)carbamothioyl)benzamide
Bos taurus
-
at pH 9.5 and 37°C
0.003458
2-chloro-N-((4-(N-(pyrimidin-2-yl)sulfamoyl)phenyl)carbamothioyl)benzamide
Bos taurus
-
at pH 9.5 and 37°C
0.000896
3,5-dinitro-N-((4-(N-(pyrimidin-2-yl)sulfamoyl)phenyl)carbamothioyl)benzamide
Bos taurus
-
at pH 9.5 and 37°C
0.000251
4-chloro-N-((4-(N-(pyrimidin-2-yl)sulfamoyl)phenyl)carbamothioyl)benzamide
Bos taurus
-
at pH 9.5 and 37°C
0.001547
4-fluoro-N-((4-(N-(pyrimidin-2-yl)sulfamoyl)phenyl)carbamothioyl)benzamide
Bos taurus
-
at pH 9.5 and 37°C
0.003291
4-methoxy-N-((4-(N-(pyrimidin-2-yl)sulfamoyl)phenyl)carbamothioyl)benzamide
Bos taurus
-
at pH 9.5 and 37°C
0.004137
KH2PO4
Bos taurus
-
at pH 9.5 and 37°C
0.08
L-phenylalanine
Bos taurus
intestinal alkaline phosphatase, pH and temperature not specified in the publication
0.00144
N'-[(Z)-(2-hydroxyphenyl)methylidene]pyridine-4-carbohydrazide
Bos taurus
intestinal alkaline phosphatase, pH and temperature not specified in the publication
0.00734
N'-[(Z)-(3-nitrophenyl)methylidene]pyridine-4-carbohydrazide
Bos taurus
intestinal alkaline phosphatase, pH and temperature not specified in the publication
0.00972
N'-[(Z)-(4-bromophenyl)methylidene]pyridine-4-carbohydrazide
Bos taurus
intestinal alkaline phosphatase, pH and temperature not specified in the publication
0.0175
N'-[(Z)-(4-fluorophenyl)methylidene]pyridine-4-carbohydrazide
Bos taurus
intestinal alkaline phosphatase, pH and temperature not specified in the publication
0.00174
N'-[(Z)-(4-methoxyphenyl)methylidene]pyridine-4-carbohydrazide
Bos taurus
intestinal alkaline phosphatase, pH and temperature not specified in the publication
0.00146
N'-[(Z)-(pyridin-3-yl)methylidene]pyridine-4-carbohydrazide
Bos taurus
intestinal alkaline phosphatase, pH and temperature not specified in the publication
0.00246
N'-[(Z)-(pyridin-4-yl)methylidene]pyridine-4-carbohydrazide
Bos taurus
intestinal alkaline phosphatase, pH and temperature not specified in the publication
0.004256
N-((4-(N-(pyrimidin-2-yl)sulfamoyl)phenyl)carbamothioyl)acetamide
Bos taurus
-
at pH 9.5 and 37°C
0.001636
N-((4-(N-(pyrimidin-2-yl)sulfamoyl)phenyl)carbamothioyl)butyramide
Bos taurus
-
at pH 9.5 and 37°C
0.000492
N-((4-(N-(pyrimidin-2-yl)sulfamoyl)phenyl)carbamothioyl)heptanamide
Bos taurus
-
at pH 9.5 and 37°C
0.001704
N-((4-(N-(pyrimidin-2-yl)sulfamoyl)phenyl)carbamothioyl)octanamide
Bos taurus
-
at pH 9.5 and 37°C
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50 - 70
-
the half-lives for the enzyme incubated in saltfree buffer at 50°C, 60°C, and 70°C is determined to be 5.3 h, 7.9 min, and 1.7 min. All the salts tested show the ability to stabilize the enzyme when incubated at 70°C
53
-
t1/2: 15 min, native enzyme
59
-
t1/2: 6 min, native enzyme
69
-
t1/2: 6 min, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride
70
-
complete inactivation after 20 min in glycine buffer, complete inactivation after 40 min in pasteurized milk
73
-
t1/2: 4 min, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride
80
-
complete inactivation after 4 min in glycine buffer, complete inactivation after 10 min in pasteurized milk
50
-
88% loss of activity after 55 min in glycine buffer, 72% loss of activity after 120 min in pasteurized milk
50
-
t1/2: 38 min, native enzyme
50
-
30 min, aout 13% loss of activity
56
-
-
56
-
50% inactivation of the placental enzyme after 10.9 min, 50% inactivation of the liver enzyme after 16.0 min
56
-
t1/2: 10 min, native enzyme
56
-
a monoclonal antibody based capture immunoassay is used to study the kinetics of alkaline phosphatase heat denaturation in bovine milk over a range 50-60°C for 5 to 60 min using a colorimetric quantification of the enzyme activity as a reference test. A denaturation midpoint is obtained at 56°8C for a 30 min heating
58
-
t1/2: 80 min, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride
58
-
t1/2: 84 min, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride
60
-
complete inactivation after 50 min in glycine buffer, 15% loss of activity after 120 min in pasteurized milk
60
-
30 min, 96.5% loss of activity
62
-
t1/2: 35 min, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride
62
-
t1/2: 37 min, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride
65
-
50% inactivation of the placental enzyme after 1.04 min, 50% inactivation of the liver enzyme after 1.32 min
65
-
t1/2: 1 min, native enzyme
65
-
t1/2: 24 min, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride
65
-
t1/2: 25 min, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride
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Stinson, R.A.; Chan, J.R.A.
Alkaline phosphatase and its function as a protein phosphatase
Adv. Protein Phosphatases
4
127-151
1987
Bos taurus, Escherichia coli, Homo sapiens, Sus scrofa
-
brenda
Coleman, J.E.; Gettins, P.
Alkaline phosphatase, solution structure, and mechanism
Adv. Enzymol. Relat. Areas Mol. Biol.
55
381-452
1983
Bacillus subtilis, Bacillus licheniformis, Bos taurus, Escherichia coli, Homo sapiens, Micrococcus sodonensis, Rattus norvegicus
brenda
Fernley, H.N.
Mammalian alkaline phosphatases
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
4
417-447
1971
Bos taurus, Equus caballus, Homo sapiens
-
brenda
Stadtman, T.C.
Alkaline phosphatases
The Enzymes, 2nd Ed. (Boyer, P. D. , Lardy, H. , Myrbck, K. , eds. )
5
55-71
1961
Bos taurus, Escherichia coli, Sus scrofa
-
brenda
Besman, M.; Coleman, J.E.
Isozymes of bovine intestinal alkaline phosphatase
J. Biol. Chem.
260
11190-11193
1985
Bos taurus
brenda
Hsu, H.H.T.; Munoz, P.A.; Barr, J.; Oppliger, I.; Morris, D.C.; Vaananen, H.K.; Tarkenton, N.; Anderson, H.C.
Purification and partial characterization of alkaline phosphatase of matrix vesicles from fetal bovine epiphyseal cartilage. Purification by monoclonal antibody affinity chromatography
J. Biol. Chem.
260
1826-1831
1985
Bos taurus
brenda
Goldstein, D.J.; Harris, H.
Human placental alkaline phosphatase differs from that of other species
Nature
280
602-605
1979
Bos taurus, Canis lupus familiaris, Cavia porcellus, Felis catus, Ovis aries, Homo sapiens, Macaca mulatta, Mesocricetus auratus, Mus musculus, Rattus norvegicus, Sus scrofa
brenda
McCarthy, A.D.; Cortizo, A.M.; Gimenez-Segura, G.; Bruzzone, L.; Etcheverry, S.B.
Non-enzymatic glycosylation of alkaline phosphatase alters its biological properties
Mol. Cell. Biochem.
181
63-69
1998
Bos taurus
brenda
Stagni, N.; Vittur, F.; de Bernard, B.
Solubility properties of alkaline phosphatase from matrix vesicles
Biochim. Biophys. Acta
761
246-251
1983
Bos taurus
brenda
Yazdanparast, R.; Khodagholi, F.
Kinetic aspects of alkaline phosphatase refolding in the presence of alpha-cyclodextrin
Arch. Biochem. Biophys.
446
11-19
2006
Bos taurus
brenda
Zhang, L.; Buchet, R.; Azzar, G.
Phosphate binding in the active site of alkaline phosphatase and the interactions of 2-nitrosoacetophenone with alkaline phosphatase-induced small structural changes
Biophys. J.
86
3873-3881
2004
Bos taurus
brenda
Terefe, N.S.; Arimi, J.M.; Van Loey, A.; Hendrickx, M.
Kinetics of the alkaline phosphatase catalyzed hydrolysis of disodium p-nitrophenyl phosphate: effects of carbohydrate additives, low temperature, and freezing
Biotechnol. Prog.
20
1467-1478
2004
Bos taurus
brenda
Siddiqui, K.S.; Poljak, A.; Cavicchioli, R.
Improved activity and stability of alkaline phosphatases from psychrophilic and mesophilic organisms by chemically modifying aliphatic or amino groups using tetracarboxy-benzophenone derivatives
Cell. Mol. Biol.
50
657-667
2004
Bos taurus, Pandalus borealis
brenda
Fadiloglu, S.; Erkmen, O.; Sekeroglu, G.
Thermal and carbon dioxide inactivation of alkaline phosphatase in buffer and milk
Food Technol. Biotechnol.
42
27-32
2004
Bos taurus
-
brenda
Sariri, R.; Ghanadzadesh, A.; Liali, E.; Sajedi, R.H.
The inhibitory effect of alcohols on alkaline phosphatase activity
Int. J. Chem. Sci.
3
16-22
2005
Bos taurus
-
brenda
Kouassi, G.K.; Anantheswaran, R.C.; Knabel, S.J.; Floros, J.D.
Effect of high-pressure processing on activity and structure of alkaline phosphatase and lactate dehydrogenase in buffer and milk
J. Agric. Food Chem.
55
9520-9529
2007
Bos taurus
brenda
Levieux, D.; Geneix, N.; Levieux, A.
Inactivation-denaturation kinetics of bovine milk alkaline phosphatase during mild heating as determined by using a monoclonal antibody-based immunoassay
J. Dairy Res.
74
296-301
2007
Bos taurus
brenda
Giocondi, M.C.; Besson, F.; Dosset, P.; Milhiet, P.E.; Le Grimellec, C.
Temperature-dependent localization of GPI-anchored intestinal alkaline phosphatase in model rafts
J. Mol. Recognit.
20
531-537
2007
Bos taurus
brenda
Sidique, S.; Ardecky, R.; Su, Y.; Narisawa, S.; Brown, B.; Millan, J.L.; Sergienko, E.; Cosford, N.D.
Design and synthesis of pyrazole derivatives as potent and selective inhibitors of tissue-nonspecific alkaline phosphatase (TNAP)
Bioorg. Med. Chem. Lett.
19
222-225
2009
Bos taurus (P09487)
brenda
Price, P.A.; Toroian, D.; Chan, W.S.
Tissue-nonspecific Alkaline Phosphatase Is Required for the Calcification of Collagen in Serum: A possible mechanism for biomineralization
J. Biol. Chem.
284
4594-4604
2009
Bos taurus
brenda
Schrenkhammer, P.; Rosnizeck, I.C.; Duerkop, A.; Wolfbeis, O.S.; Schaeferling, M.
Time-resolved fluorescence-based assay for the determination of alkaline phosphatase activity and application to the screening of its inhibitors
J. Biomol. Screen.
13
9-16
2008
Bos taurus (P19111)
brenda
Ziegler, A.J.; Florian, J.; Ballicora, M.A.; Herlinger, A.W.
Alkaline phosphatase inhibition by vanadyl-beta-diketone complexes: electron density effects
J. Enzyme Inhib. Med. Chem.
24
22-28
2009
Bos taurus
brenda
Mark, S.S.; Stolper, S.I.; Baratti, C.; Park, J.Y.; Taku, M.A.; Santiago-Aviles, J.J.; Kricka, L.J.
Bioconjugation of alkaline phosphatase to mechanically processed, aqueous suspendible electrospun polymer nanofibers for use in chemiluminescent detection assays
Macromol. Biosci.
8
484-498
2008
Bos taurus (P19111)
brenda
Atyaksheva, L.F.; Chukhrai, E.S.; Poltorak, O.M.
The catalytic properties of alkaline phosphatases under various conditions
Russ. J. Phys. Chem. A
82
1947-1951
2008
Bos taurus, Escherichia coli, Gallus gallus (Q92058)
-
brenda
Li, L.; Chang, L.; Pellet-Rostaing, S.; Liger, F.; Lemaire, M.; Buchet, R.; Wu, Y.
Synthesis and evaluation of benzo[b]thiophene derivatives as inhibitors of alkaline phosphatases
Bioorg. Med. Chem.
17
7290-7300
2009
Bos taurus, Sus scrofa
brenda
Iqbal, J.
An enzyme immobilized microassay in capillary electrophoresis for characterization and inhibition studies of alkaline phosphatases
Anal. Biochem.
414
226-231
2011
Bos taurus
brenda
Yang, Z.; Liu, X.; Chen, C.; Halling, P.
Hofmeister effects on activity and stability of alkaline phosphatase
Biochim. Biophys. Acta
1804
821-828
2010
Bos taurus
brenda
Sekiguchi, S.; Hashida, Y.; Yasukawa, K.; Inouye, K.
Effects of amines and aminoalcohols on bovine intestine alkaline phosphatase activity
Enzyme Microb. Technol.
49
171-176
2011
Bos taurus
brenda
Chaudhuri, G.; Chatterjee, S.; Venu-Babu, P.; Ramasamy, K.; Thilagaraj, W.R.
Kinetic behaviour of calf intestinal alkaline phosphatase with pNPP
Indian J. Biochem. Biophys.
50
64-71
2013
Bos taurus
brenda
Sajid-Ur-Rehman, X.; Saeed, A.; Saddique, G.; Ali Channar, P.; Ali Larik, F.; Abbas, Q.; Hassan, M.; Raza, H.; Fattah, T.A.; Seo, S.Y.
Synthesis of sulfadiazinyl acyl/aryl thiourea derivatives as calf intestinal alkaline phosphatase inhibitors, pharmacokinetic properties, lead optimization, Lineweaver-Burk plot evaluation and binding analysis
Bioorg. Med. Chem.
26
3707-3715
2018
Bos taurus
brenda
Channar, P.A.; Shah, S.J.; Hassan, S.; Nisa, Z.U.; Lecka, J.; Sevigny, J.; Bajorath, J.; Saeed, A.; Iqbal, J.
Isonicotinohydrazones as inhibitors of alkaline phosphatase and ecto-5-nucleotidase
Chem. Biol. Drug Des.
89
365-370
2017
Bos taurus (P09487), Bos taurus (P19111), Bos taurus
brenda
Zhang, Q.; Zhang, C.; Yang, M.; Yu, D.; Yu, C.
Pyrophosphate as substrate for alkaline phosphatase activity A convenient flow-injection chemiluminescence assay
Luminescence
32
1150-1156
2017
Bos taurus
brenda