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Information on EC 3.1.3.1 - alkaline phosphatase and Organism(s) Homo sapiens and UniProt Accession P05186

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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.3 Phosphoric-monoester hydrolases
                3.1.3.1 alkaline phosphatase
IUBMB Comments
Wide specificity. Also catalyses transphosphorylations. The human placental enzyme is a zinc protein. Some enzymes hydrolyse diphosphate (cf. EC 3.6.1.1 inorganic diphosphatase)
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Select one or more organisms in this record:
This record set is specific for:
Homo sapiens
UNIPROT: P05186
Word Map
The expected taxonomic range for this enzyme is: Eukaryota, Archaea, Bacteria
The taxonomic range for the selected organisms is: Homo sapiens
Synonyms
AIP, alkaline p-nitrophenylphosphate phosphatase, alkaline phenyl phosphatase, alkaline phosphatase, alkaline phosphatase 4, alkaline phosphatase F I, alkaline phosphatase F II, alkaline phosphatase HaALP1, alkaline phosphatase HaALP2, alkaline phosphatase HvmALP 1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
alkaline phenyl phosphatase
-
-
-
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alkaline phosphohydrolase
-
-
-
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alkaline phosphomonoesterase
-
-
-
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ALPP
247
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AP-TNAP
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-
-
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APase
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-
-
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APASED
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-
-
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BAB
247
-
BALP
247
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BC6
-
-
-
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bone alkaline phosphatase
247
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bone specific alkaline phosphatase
247
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EAP
-
-
-
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germ cell alkaline phosphatase
247
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Germ-cell alkaline phosphatase
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-
-
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glycerophosphatase
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-
-
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H-AP
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-
-
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High molecular weight phosphatase
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-
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intestinal alkaline phosphatase
L-AP
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-
-
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Liver/bone/kidney isozyme
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-
-
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Low molecular weight phosphatase
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-
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M-ALP
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-
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MSCA-1
282058
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Nagao isozyme
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non-specific alkaline phosphatase
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PALP
247
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phosphatase, alkaline
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-
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phosphomonoesterase
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placental alkaline phosphatase
247
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PLAP-like
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RAN1
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-
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Regan isozyme
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-
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salivary alkaline phosphatase
247
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SEAP
247
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secreted alkaline phosphatase
247
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strensiq
247
recombinant enzyme, previously known as asfotase-alfa
tissue non-specific alkaline phosphatase
tissue nonspecific alkaline phosphatase
247
-
tissue-nonspecific alkaline phosphatase
TNSALP
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a phosphate monoester + H2O = an alcohol + phosphate
show the reaction diagram
SYSTEMATIC NAME
IUBMB Comments
phosphate-monoester phosphohydrolase (alkaline optimum)
Wide specificity. Also catalyses transphosphorylations. The human placental enzyme is a zinc protein. Some enzymes hydrolyse diphosphate (cf. EC 3.6.1.1 inorganic diphosphatase)
CAS REGISTRY NUMBER
COMMENTARY hide
9001-78-9
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
-
-
-
?
alpha-naphthyl phosphate + H2O
alpha-naphthol + phosphate
show the reaction diagram
-
-
-
-
?
2'-AMP + H2O
adenosine + phosphate
show the reaction diagram
-
-
-
-
?
2-chloro-5-(4-methoxyspiro (1,2-dioxetane-3,2'-(5'-chloro)tricyclo[3.3.1.13,7]decan)-4-yl)phenyl phosphate + H2O
2-chloro-5-(4-methoxyspiro (1,2-dioxetane-3,2'-(5'-chloro)tricyclo[3.3.1.13,7]decan)-4-yl)phenol + phosphate
show the reaction diagram
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CDP-star
-
-
?
2-naphthyl phosphate + H2O
2-naphthol + phosphate
show the reaction diagram
-
-
-
-
?
2-phosphoglycerate + H2O
glycerol + phosphate
show the reaction diagram
-
-
-
-
?
3'-AMP + H2O
adenosine + phosphate
show the reaction diagram
-
-
-
-
?
3-phosphoglycerate + H2O
glycerol + phosphate
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl phosphate + H2O
4-methylumbelliferone + phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
5'-AMP + H2O
adenosine + phosphate
show the reaction diagram
-
-
-
-
?
5'-dTMP + H2O
?
show the reaction diagram
-
-
-
-
?
5'-UMP + H2O
uridine + phosphate
show the reaction diagram
-
-
-
-
?
ADP + H2O
?
show the reaction diagram
-
-
-
-
?
AMP + H2O
adenosine + phosphate
show the reaction diagram
-
-
-
-
?
ATP + H2O
?
show the reaction diagram
-
-
-
-
?
diphosphate + H2O
2 phosphate
show the reaction diagram
glucose 1-phosphate + H2O
glucose + phosphate
show the reaction diagram
-
-
-
-
?
glycogen synthase + H2O
?
show the reaction diagram
-
-
-
-
?
histone + H2O
?
show the reaction diagram
-
32P-labelled
-
-
?
indoxyl phosphate + nitroblue tetrazolium chloride
nitrioblue diformazan + phosphate
show the reaction diagram
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
p-toluidinium 5-bromo-4-chloro-3-indolyl phosphate + H2O
?
show the reaction diagram
-
-
-
-
?
phenyl phosphate + H2O
phenol + phosphate
show the reaction diagram
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-
-
-
?
phospho-DL-Thr + H2O
phosphate + Thr
show the reaction diagram
-
-
-
-
?
phospho-DL-Tyr + H2O
phosphate + Tyr
show the reaction diagram
-
-
-
-
?
phospho-Ser histone + H2O
?
show the reaction diagram
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-
-
-
?
phospho-Ser-casein + H2O
?
show the reaction diagram
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-
-
-
?
phosphoethanolamine + H2O
phosphate + ethanolamine
show the reaction diagram
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-
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-
?
phosphorylase kinase + H2O
?
show the reaction diagram
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-
-
-
?
phosphorylated acetyl-CoA carboxylase + H2O
acetyl-CoA carboxylase + phosphate
show the reaction diagram
-
-
-
-
?
phosphoserine + H2O
phosphate + Ser
show the reaction diagram
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-
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-
?
phosvitin + H2O
?
show the reaction diagram
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-
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-
?
protamine + H2O
?
show the reaction diagram
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-
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-
?
pyridoxal 5'-phosphate + H2O
?
show the reaction diagram
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-
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-
?
pyridoxal 5'-phosphate + H2O
pyridoxal + phosphate
show the reaction diagram
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-
-
?
thiamin diphosphate + H2O
thiamin phosphate + phosphate
show the reaction diagram
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-
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?
triphosphate + H2O
diphosphate + phosphate
show the reaction diagram
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-
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?
UDP + H2O
?
show the reaction diagram
-
-
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-
?
UMP + H2O
uridine + phosphate
show the reaction diagram
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-
-
-
?
UTP + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
diphosphate + H2O
2 phosphate
show the reaction diagram
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-
-
?
pyridoxal 5'-phosphate + H2O
pyridoxal + phosphate
show the reaction diagram
-
-
-
?
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
-
stimulates glycogen synthase phosphatase activity
Zn
-
enzyme contains Zn; enzyme from placenta conains 2-3 Zn per dimer
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,4-dimethoxy-2-methylbenzene
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1-(3,4-dihydroxyphenyl)-2-(1H-imidazol-1-yl)ethanone
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1-(3,4-dihydroxyphenyl)-2-(2-ethyl-1H-imidazol-1-yl)ethanone
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1-(3,4-dihydroxyphenyl)-2-(2-methyl-1H-benzimidazol-1-yl)ethanone
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1-(3,4-dihydroxyphenyl)-2-(2-methyl-1H-imidazol-1-yl)ethanone
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1-(3,4-dihydroxyphenyl)-2-(2-phenyl-1H-imidazol-1-yl)ethanone
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1-(3,4-dihydroxyphenyl)-2-(4-methyl-1H-imidazol-1-yl)ethanone
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1-(3,4-dihydroxyphenyl)-2-(4-methyl-1H-pyrazol-1-yl)ethanone
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1-(3,4-dihydroxyphenyl)-2-(4H-1,2,4-triazol-4-yl)ethanone
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1-(3,4-dihydroxyphenyl)-2-(5,6-dimethyl-1H-benzimidazol-1-yl)ethanone
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1-(3,4-dihydroxyphenyl)-2-(propan-2-ylamino)ethanone
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1-(3,4-dihydroxyphenyl)-2-[(4,6-dimethylpyrimidin-2-yl)sulfanyl]ethanone
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1-(3,4-dihydroxyphenyl)-2-[(4-methyl-5-phenyl-4H-1,2,4-triazol-3-yl)sulfanyl]ethanone
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1-(3,4-dihydroxyphenyl)-2-[[1-(4-methoxyphenyl)-1H-tetrazol-5-yl]sulfanyl]ethanone
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1-bromotetramisole
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1-chloro-4-ethoxy-2-methylbenzene
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1-chloro-4-methoxy-2-methylbenzene
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1-fluoro-4-methoxybenzene
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2,5-dimethoxy-N-(pyridin-3-yl)benzenesulfonamide
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2,5-dimethoxy-N-(quinolin-3-yl)benzenesulfonamide
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2-(1-methylhydrazenyl)-7-trifluoromethyl-5H-1,3,4-thiadiazolo[3,2-a]pyrimidin-5-one
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2-(1H-benzimidazol-1-yl)-1-(3,4-dihydroxyphenyl)ethanone
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2-(1H-benzimidazol-2-ylamino)-1-(3,4-dihydroxyphenyl)ethanone
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2-(2-ethoxyphenyl)-7-trifluoromethyl-5H-1,3,4-thiadiazolo[3,2-a]pyrimidin-5-one
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2-(2-fluorophenyl)-7-trifluoromethyl-5H-1,3,4-thiadiazolo[3,2-a]pyrimidin-5-one
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2-(2-methoxyphenyl)-7- trifluoromethyl-5H-1,3,4-thiadiazolo[3,2-a]pyrimidin-5-one
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2-(3,4-di-methylphenyl)-7-trifluoromethyl-5H-1,3,4-thiadiazolo[3,2-a]pyrimidin-5-one
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2-(3,5-di-methoxyphenyl)-7-trifluoromethyl-5H-1,3,4-thiadiazolo[3,2-a]pyrimidin-5-one
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2-(3,5-di-methylphenyl)-7-trifluoromethyl-5H-1,3,4-thiadiazolo[3,2-a]pyrimidin-5-one
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2-(3-cyanophenyl)-7-trifluoromethyl-5H-1,3,4-thiadiazolo[3,2-a]pyrimidin-5-one
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2-(3-fluoromethylphenyl)-7-trifluoromethyl-5H-1,3,4-thiadiazolo[3,2-a]pyrimidin-5-one
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2-(3-fluorophenyl)-7-trifluoromethyl-5H-1,3,4-thiadiazolo[3,2-a]pyrimidin-5-one
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2-(3-methoxyphenyl)amino-7-trifluoromethyl-5H-1,3,4-thiadiazolo[3,2-a]pyrimidin-5-one
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2-(3-nitrophenyl)-7-trifluoromethyl-5H-1,3,4-thiadiazolo[3,2-a]pyrimidin-5-one
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2-(3-phenylphenyl)-7-trifluoromethyl-5H-1,3,4-thiadiazolo[3,2-a]pyrimidin-5-one
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2-(4-aminophenyl)amino-7-trifluoromethyl-5H-1,3,4-thiadiazolo[3,2-a]pyrimidin-5-one
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2-(4-bromo-1H-imidazol-1-yl)-1-(3,4-dihydroxyphenyl)ethanone
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2-(4-bromo-2-methyl-1H-imidazol-1-yl)-1-(3,4-dihydroxyphenyl)ethanone
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2-(4-ethoxyphenyl)-7-trifluoromethyl-5H-1,3,4-thiadiazolo[3,2-a]pyrimidin-5-one
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2-(4-ethoxyphenyl)amino-7-trifluoromethyl-5H-1,3,4-thiadiazolo[3,2-a]pyrimidin-5-one
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2-(4-ethylphenyl)-7-trifluoromethyl-5H-1,3,4-thiadiazolo[3,2-a]pyrimidin-5-one
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2-(4-i-propylphenyl)amino-7-trifluoromethyl-5H-1,3,4-thiadiazolo[3,2-a]pyrimidin-5-one
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2-(4-methoxylphenyl)-7-trifluoromethyl-5H-1,3,4-thiadiazolo[3,2-a]pyrimidin-5-one
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2-(4-methylphenyl)-7-trifluoromethyl-5H-1,3,4-thiadiazolo[3,2-a]pyrimidin-5-one
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2-(4-trifluoromethoxylphenyl)-7-trifluoromethyl-5H-1,3,4-thiadiazolo[3,2-a]pyrimidin-5-one
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2-allylamino-7-trifluoromethyl-5H-1,3,4-thiadiazolo[3,2-a]pyrimidin-5-one
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2-chloro-1,4-dimethoxybenzene
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2-di-n-butylamino-7-trifluoromethyl-5H-1,3,4-thiadiazolo[3,2-a]pyrimidin-5-one
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2-ethoxy-5-methyl-N-(pyridin-3-yl)benzenesulfonamide
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2-methoxy-4-nitro-N-(pyridin-3-yl)benzenesulfonamide
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2-methoxy-4-nitro-N-(quinolin-3-yl)benzenesulfonamide
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2-methoxy-5-methyl-N-(pyridin-3-yl)benzenesulfonamide
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2-methoxy-5-methyl-N-(quinolin-3-yl)benzenesulfonamide
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2-n-butylamino-7-trifluoromethyl-5H-1,3,4-thiadiazolo[3,2-a]pyrimidin-5-one
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2-n-pentylamino-7-trifluoromethyl-5H-1,3,4-thiadiazolo[3,2-a]pyrimidin-5-one
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2-n-propylamino-7-trifluoromethyl-5H-1,3,4-thiadiazolo[3,2-a]pyrimidin-5-one
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2-phenyl-7-trifluoromethyl-5H-1,3,4-thiadiazolo[3,2-a]pyrimidin-5-one
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2-[2-(dimethylamino)ethyl]amino-7-trifluoromethyl-5H-1,3,4-thiadiazolo[3,2-a]pyrimidin-5-one
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2-[[2-(3,4-dihydroxyphenyl)-2-oxoethyl]sulfanyl]-4-(methoxymethyl)-6-methylpyridine-3-carbonitrile
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3-[2-(3,4-dihydroxyphenyl)-2-oxoethyl]-6,7-dimethoxy-2-benzofuran-1(3H)-one
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4-bromo-2,5-dimethoxy-N-(pyridin-3-yl)benzenesulfonamide
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4-methoxy-1,2-dimethylbenzene
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4-[[(4,6-dimethylpyrimidin-2-yl)amino]methyl]benzene-1,2-diol
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5-bromo-2-methoxy-N-(quinolin-3-yl)benzenesulfonamide
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5-chloro-2-ethoxy-N-(pyridin-3-yl)benzenesulfonamide
-
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arsenate
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Be2+
-
-
Ca2+
-
10 mM, glycogen synthase phosphatase activity
Cys
-
uncompetitive inhibition of phosphatase actvity, mixed-competitive inhibition of inorganic pyrophosphatase activity. Inorganic pyrophosphatase activity is inhibited more than phosphatase activity. Ca2+ and Mg2+ ion concentrations may regulate this inhibition
diethyl-p-nitrophenyl phosphate
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-
F-
-
50 mM KF, 13% inhibition of p-nitrophenyl phosphate hydrolysis, 50% inhibition of dephosphorylation of P-labelled histones
heparin
-
histone phosphatase activity
histidine
-
-
L-amino acids
specific uncompetitive inhibition, molecular mechanism involves Arg166 and Glu429
L-homoarginine
L-leucine
-
allozyme D is highly sensitive and shows uncompetitive inhibition, allozymes S and F are less sensitive, allozymes SD and FD, mixed type, respond in an intermediate fashion to inhibition
L-Phe-Gly-Gly
L-phenylalanine
inhibitor of intestinal alkaline phosphatase
Leu
-
L-Leu, uncompetitive, D-Leu with greatly decreased efficiencies
leucinamide
-
-
leucinol
-
-
levamisole
Mg2+
-
-
NaCN
-
-
NADH
-
-
NEM
-
very slowly
p-hydroxymercuribenzoate
-
reactivation by DTT and 2-mercaptoethanol
Phenanthroline
-
-
phenyl phosphonate
-
competitive
phenylalanine
-
-
phenylphosphonate
-
10 mM, 47% inhibition of p-nitrophenyl phosphate hydrolysis, 80% inhibition of dephosphorylation of P-labelled histones
phosphate
-
-
polylysine
-
histone phosphatase activity
protamine
-
histone phosphatase activity
Trimetaphosphate
-
-
vanadate
-
-
Zn2+
-
above 0.02 mM
additional information
-
no inhibition at 10 mM by L-phenylalanine at 10 mM for all three isozymes
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
albumin
-
3fold stimulation of hydrophilic enzyme, slight stimulation of amphiphilic enzyme
-
phosphatidylcholine
-
1fold stimulation of hydrophilic enzyme form
phosphatidylethanolamine
-
1fold stimulation of hydrophilic enzyme form
phosphatidylinositol
-
1.5fold stimulation of hydrophilic enzyme form
phosphatidylserine
-
1fold stimulation of amphiphilic enzyme form, 3fold stimulation of hydrophilic enzyme form