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Reaction Schemes
endonucleolytic cleavage of RNA precursor into two, leaving 5'-hydroxy and 3'-phosphate groups
Synonyms
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endonucleolytic cleavage of RNA precursor into two, leaving 5'-hydroxy and 3'-phosphate groups
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hydrolysis of phosphoric ester
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tRNA precursors + H2O
3'-phosphooligonucleotides
tRNA precursors + H2O
3'-phosphooligonucleotides
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Substrates: cleavage within a possible stem-loop at the border between ds and ss regions
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tRNA precursors + H2O
3'-phosphooligonucleotides
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Substrates: cleavage near the 3'-end of tRNAs
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tRNA precursors + H2O
3'-phosphooligonucleotides
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Substrates: preferential cleavage between a cytosine and an adenine moiety
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tRNA precursors + H2O
3'-phosphooligonucleotides
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Substrates: -
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tRNA precursors + H2O
3'-phosphooligonucleotides
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Substrates: -
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K+
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stimulating, optimum: 150-200 mM
Na+
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stimulating, optimum: 150-200 mM
NH4+
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stimulating, optimum: 150-200 mM
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3',5'-oligoadenylate
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50% activation at 0.000004 M
2',5'-Oligoadenylate
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2',5'-Oligoadenylate
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50% activation at 0.0000008 M
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Virus Diseases
A mouse cell line, which is unprotected by interferon against lytic virus infection, lacks ribonuclease F activity.
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60
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enzyme is active up to this temperature, above: spontaneous substrate-breakdown
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brenda
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brenda
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cell line
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Highest Expressing Human Cell Lines
Filter by:
Cell Line Links
Gene Links
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90
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slowly inactivated at this temperature, after 50 min 50% of activity remained
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-70°C, 0.44 mg protein per ml, 1.5 ml aliquots
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Gurevitz, M.; Watson, N.; Apirion, D.
A cleavage site of ribonuclease F. A putative processing endoribonuclease from Escherichia coli
Eur. J. Biochem.
124
553-559
1982
Escherichia coli
brenda
Watson, N.; Apirion, D.
Ribonuclease F, a putative processing endoribonuclease from Escherichia coli
Biochem. Biophys. Res. Commun.
103
543-551
1981
Escherichia coli
brenda
Banerjee, D.K.; Baksi, K.; Gottesmann, M.M.
Genetic evidence that action of cAMP-dependent protein kinase is not an obligatory step for antiviral and antiproliferative effects of human interferon in chinese hamster ovary cells
Virology
129
230-238
1983
Cricetulus griseus
brenda
Rappoport, S.; Arad, G.; Lapidot, Y.; Panet, A.
Activation of ribonuclease F by the two isomers (2-5)oligoadenylate and (3-5)oligoadenylate
FEBS Lett.
149
47-50
1982
Mus musculus
brenda
Epstein, D.A.; Czarniecki, C.W.; Jacobsen, H.; Friedmann, R.M.; Panet, A.
A mouse cell line, which is unprotected by interferon against lytic virus infection, lacks ribonuclease F activity
Eur. J. Biochem.
118
9-15
1981
Mus musculus
brenda
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