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Information on EC 3.1.26.3 - ribonuclease III and Organism(s) Homo sapiens and UniProt Accession Q9NRR4

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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.26 Endoribonucleases producing 5'-phosphomonoesters
                3.1.26.3 ribonuclease III
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This record set is specific for:
Homo sapiens
UNIPROT: Q9NRR4 not found.
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The taxonomic range for the selected organisms is: Homo sapiens
The enzyme appears in selected viruses and cellular organisms
Synonyms
dicer, dicer1, drosha, dicer-2, rnt1p, ribonuclease iii, dicer-1, rnase d, dcr-1, rnase3, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Hs-Drosha
-
ribonuclease III
-
RNase III
-
Dicer
Dicer 1
-
-
Dicer1
-
-
HCS protein
-
-
-
-
nuclease, ribo-, D
-
-
-
-
p241
-
-
-
-
ribonuclease D
-
-
-
-
ribonuclease III
ribonuclease type III
-
-
RNase D
-
-
-
-
RNase III
RNase III domain A
-
-
RNase III endonuclease
-
RNase O
-
-
-
-
RNase3
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Endonucleolytic cleavage to a 5'-phosphomonoester
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric diester
-
hydrolysis of phosphoric ester
-
-
-
-
hydrolysis of phosphoric diester
CAS REGISTRY NUMBER
COMMENTARY hide
78413-14-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
double-stranded RNA + H2O
5'-phosphooligonucleotides
show the reaction diagram
dsRNA + H2O
mature RNA
show the reaction diagram
single-stranded RNA + H2O
5'-phosphooligonucleotides
show the reaction diagram
-
-
-
-
?
tRNA + H2O
5'-phosphooligonucleotides
show the reaction diagram
-
HIV-1 RT displays the same cleavage specificity as RNase D
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
double-stranded RNA + H2O
5'-phosphooligonucleotides
show the reaction diagram
dsRNA + H2O
mature RNA
show the reaction diagram
-
reshaping of fully or partially double-stranded RNA precursors into mature RNAs involved in pre-mRNA splicing, RNA modification, translation, gene silencing, and regulation of developmental timing
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
activates
Mn2+
-
activates
Ni2+
-
activates
additional information
-
the enzyme requires a divalent metal ion for catalysis
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ethidium bromide
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
-
the activity of Drosha is modulated between different cell types or differentiation stages
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
drosha
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
physiological function
additional information
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
RNC_HUMAN
1374
0
159316
Swiss-Prot
Mitochondrion (Reliability: 5)
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
recombinant RNase IIIb-dsRBD fragment, crystal structure analysis, and gel filtration data
homodimer
-
the Drosha polypeptide possesses tandem RNase III domains and a C-terminal dsRBD. The RNase III domains form an intramolecular pseudodimer with two catalytic sites. The Drosha dsRBD structure shows an alpha1-alpha1 loop element with a dynamic, extended structure
monomer
-
biochemical experiments indicate that Drosha functions as a monomer, with its ribonuclease domains forming an internal dimer structure
additional information
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant protein including His-tag by sitting drop vapor diffusion method
recombinant RNase IIIb-dsRBD fragment by sitting drop vapor diffusion method
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D1709A
strongly reduced dsRNA cleavage activity
D1713A
no significant effect on the cleavage activity
D1713K
no significant effect on the cleavage activity
D1810A
reduced dsRNA cleavage activity
DELTA1787-1799
no significant effect on the cleavage activity
E1705A
reduced dsRNA cleavage activity
E1813A
strongly reduced dsRNA cleavage activity
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
of the human immunodeficiency virus type 1 reverse transcriptase
-
purified on a Ni2+-NTA superflow column
-
recombinant C-terminally His6-tagged enzyme from Escherichia coli BL21(DE3) Codon Plus by metal affinity chromatography
-
recombinant protein
recombinant protein using His-tag
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of full-length cDNA of DICER1 protein from HeLa cell total RNA, and expression of N-terminally FLAG-tagged DICER1 protein in 293T cells
expressed as a 6×His-tagged fragment in Escherichia coli BL21 strain
-
expression of His-tag fused RNase IIIb-dsRBD fragment of human Dicer (residues 1649-1922) in Escherichia coli HT115
recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli BL21(DE3) Codon Plus
-
region encoding the RNase IIIb domain (residues 1660-1852) expressed as His-tag fusion protein in Escherichia coli BL21 star (DE3)
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Dunn, J.J
Ribonuclease III
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
15
485-499
1982
Bos taurus, Escherichia coli, Homo sapiens, Mus musculus
-
Manually annotated by BRENDA team
Ben-Artzi, H.; Zeelon, E.; Gorecki, M.; Panet, A.
Double-stranded RNA-dependent RNase activity associated with human immunodeficiency virus type 1 reverse transcriptase
Proc. Natl. Acad. Sci. USA
89
927-931
1992
Homo sapiens
Manually annotated by BRENDA team
Hostomsky, Z.; Hudson, G.O.; Rahmati, S.; Hostomska, Z.
RNase D, a reported new activity associated with HIV-1 reverse transcriptase, displays the same cleavage specificity as Escherichia coli RNase III
Nucleic Acids Res.
20
5819-5824
1992
Homo sapiens
Manually annotated by BRENDA team
Zamore, P.D.
Thirty-three years later, a glimpse at the ribonuclease III active site
Mol. Cell
8
1158-1160
2001
Aquifex aeolicus, Arabidopsis thaliana, Caenorhabditis elegans, Drosophila melanogaster, Escherichia coli, Homo sapiens
Manually annotated by BRENDA team
Zhang, H.; Kolb, F.A.; Jaskiewicz, L.; Westhof, E.; Filipowicz, W.
Single processing center models for human Dicer and bacterial RNase III
Cell
118
57-68
2004
Escherichia coli, Homo sapiens
Manually annotated by BRENDA team
Ji, X.
Structural basis for non-catalytic and catalytic activities of ribonuclease III
Acta Crystallogr. Sect. D
62
933-940
2006
Thermotoga maritima, Aquifex aeolicus (O67082), Escherichia coli (P0A7Y0), Saccharomyces cerevisiae (Q02555), Homo sapiens (Q9NRR4), Homo sapiens (Q9UPY3)
Manually annotated by BRENDA team
Takeshita, D.; Zenno, S.; Lee, W.C.; Nagata, K.; Saigo, K.; Tanokura, M.
Crystallization and preliminary X-ray analysis of the C-terminal RNase III domain of human Dicer
Acta Crystallogr. Sect. F
62
402-404
2006
Homo sapiens (Q9UPY3), Homo sapiens
Manually annotated by BRENDA team
Takeshita, D.; Zenno, S.; Lee, W.C.; Nagata, K.; Saigo, K.; Tanokura, M.
Homodimeric structure and double-stranded RNA cleavage activity of the C-terminal RNase III domain of human dicer
J. Mol. Biol.
374
106-120
2007
Homo sapiens (Q9UPY3), Homo sapiens
Manually annotated by BRENDA team
MacRae, I.J.; Doudna, J.A.
Ribonuclease revisited: structural insights into ribonuclease III family enzymes
Curr. Opin. Struct. Biol.
17
138-145
2007
Aquifex aeolicus, Saccharomyces cerevisiae, Escherichia coli, Giardia intestinalis, Homo sapiens
Manually annotated by BRENDA team
Dincbas-Renqvist, V.; Pepin, G.; Rakonjac, M.; Plante, I.; Ouellet, D.L.; Hermansson, A.; Goulet, I.; Doucet, J.; Samuelsson, B.; Radmark, O.; Provost, P.
Human Dicer C-terminus functions as a 5-lipoxygenase binding domain
Biochim. Biophys. Acta
1789
99-108
2009
Homo sapiens
Manually annotated by BRENDA team
Jin, L.; Kryukov, K.; Suzuki, Y.; Imanishi, T.; Ikeo, K.; Gojobori, T.
The evolutionary study of small RNA-directed gene silencing pathways by investigating RNase III enzymes
Gene
435
1-8
2009
Caenorhabditis elegans, Cryptococcus neoformans, Dictyostelium discoideum, Drosophila melanogaster, Enterococcus faecalis, Homo sapiens
Manually annotated by BRENDA team
Ando, Y.; Maida, Y.; Morinaga, A.; Burroughs, A.M.; Kimura, R.; Chiba, J.; Suzuki, H.; Masutomi, K.; Hayashizaki, Y.
Two-step cleavage of hairpin RNA with 5' overhangs by human DICER
BMC Mol. Biol.
12
6
2011
Homo sapiens (Q9UPY3), Homo sapiens
Manually annotated by BRENDA team
Lien, P.C.; Kuo, P.H.; Chen, C.J.; Chang, H.H.; Fang, S.L.; Wu, W.S.; Lai, Y.K.; Pai, T.W.; Chang, M.D.
In silico prediction and in vitro characterization of multifunctional human RNase3
BioMed Res. Int.
2013
170398
2013
Homo sapiens
Manually annotated by BRENDA team
Dewi, D.L.; Ishii, H.; Haraguchi, N.; Nishikawa, S.; Kano, Y.; Fukusumi, T.; Ozaki, M.; Saito, T.; Sakai, D.; Satoh, T.; Doki, Y.; Mori, M.
Dicer 1, ribonuclease type III modulates a reprogramming effect in colorectal cancer cells
Int. J. Mol. Med.
29
1060-1064
2012
Homo sapiens
Manually annotated by BRENDA team
Johanson, T.M.; Lew, A.M.; Chong, M.M.
MicroRNA-independent roles of the RNase III enzymes Drosha and Dicer
Open Biology
3
130144
2013
Arabidopsis thaliana, Saccharomyces cerevisiae, Caenorhabditis elegans, Drosophila melanogaster, Escherichia coli, Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Nicholson, A.W.
Ribonuclease III mechanisms of double-stranded RNA cleavage
Wiley Interdiscip. Rev. RNA
5
31-48
2013
Saccharomyces cerevisiae, Escherichia coli, Homo sapiens, Schizosaccharomyces pombe, Giardia intestinalis (A8BQJ3), Aquifex aeolicus (O67082), Mycobacterium tuberculosis (P9WH03), Thermotoga maritima (Q9X0I6), Mycobacterium tuberculosis H37Rv (P9WH03)
Manually annotated by BRENDA team
Xu, W.; Liu, Y.; Liu, Y.; Liu, L.; Zhan, Y.; Zhuang, C.; Lin, J.; Chen, M.; Li, J.; Cai, Z.; Huang, W.; Zhang, Y.
Artificial small RNA for sequence specific cleavage of target RNA through RNase III endonuclease Dicer
Oncotarget
7
54549-54554
2016
Homo sapiens (Q9UPY3)
Manually annotated by BRENDA team