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Information on EC 3.1.26.3 - ribonuclease III and Organism(s) Bacillus subtilis and UniProt Accession O31418

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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.26 Endoribonucleases producing 5'-phosphomonoesters
                3.1.26.3 ribonuclease III
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Bacillus subtilis
UNIPROT: O31418 not found.
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Word Map
The taxonomic range for the selected organisms is: Bacillus subtilis
The enzyme appears in selected viruses and cellular organisms
Synonyms
dicer, dicer1, drosha, dicer-2, rnt1p, ribonuclease iii, dicer-1, rnase d, dcr-1, rnase3, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
mini-III
unusual member of the RNase III family of enzymes, catalyses 23S ribosomal RNA maturation in Bacillus subtilis. Defines a fourth class of naturally occurring functional RNase III enzymes, which consists of just the catalytic domain
HCS protein
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-
-
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mini-III
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a fourth class of RNase III, consisting only of the RNase III catalytic domain and functioning in the maturation of the 23S rRNA in Bacillus subtilis
nuclease, ribo-, D
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p241
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-
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ribonuclease D
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-
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ribonuclease III
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RNase D
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-
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RNase O
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-
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-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
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-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
78413-14-6
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dsRNA + H2O
mature RNA
show the reaction diagram
-
-
-
?
pre-23S rRNA + H2O
mature 23S rRNA
show the reaction diagram
-
-
-
?
double-stranded RNA + H2O
5'-phosphooligonucleotides
show the reaction diagram
mature 23S rRNA
23S pre-rRNA + H2O
show the reaction diagram
-
-
-
-
?
mRNA transcripts + H2O
5'-phosphooligonucleotides
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
double-stranded RNA + H2O
5'-phosphooligonucleotides
show the reaction diagram
-
cleaves multimeric tRNA precursor at the spacer region, also involved in processing of precursor rRNA, hnRNA and early T7-mRNA. Also cleaves double-stranded DNA and single-stranded RNA
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-
?
additional information
?
-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Double-stranded RNA
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inhibits cleavage of single-stranded RNA
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain W168 and strain yazCdd (SSB1044)
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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in the absence of RNase III, only trace amounts of 30S rRNA precursor are observed in Bacillus subtilis. A significant increase in the steady-state levels of the type I toxin mRNA txpA occurs in strains depleted for RNase III. Expression of the txpA and yonT toxin mRNAs account for the lethality of the Skin and SPbeta prophages in RNase III mutants
metabolism
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the enzymes that catalyze the final steps of rRNA maturation, RNase J1, Mini-III and RNase M5, function efficiently without prior RNase III action
physiological function
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the enzyme plays a role in the mechanism of RatA mediated degradation of txpA mRNA both in vivo and in vitro and in cleaving double-stranded RNA in many biological systems, it is involved in ribosomal RNA maturation and mRNA turnover. In contrast to other bacteria, the enzyme is essential in Bacillus subtilis, it protects the organism from the expression of toxin genes borne by two prophages, through antisense RNA, although it is not responsible for the stabilities of antisense-RNAs. Degradation of type I toxin txpA is dependent on both RatA and RNase III. The organism uses RNase III or its homologues as part of viral defense or viral accommodation mechanisms
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
17100
2 * 17100, His-tagged monomer, calculated
38100
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
2 * 17100, His-tagged monomer, calculated
additional information
-
consisting only of the RNase III catalytic domain
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
gel filtration analysis on a Superdex 75 10/30 column
of the native and recombinant protein
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
overexpression of the rncS gene in Escherichia coli
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Panganiban, A.T.; Whiteley, H.R.
Bacillus subtilis RNAase III cleavage sites in phage SP82 early mRNA
Cell
33
907-913
1983
Bacillus subtilis, Escherichia coli
Manually annotated by BRENDA team
Oguro, A.; Kakeshita, H.; Nakamura, K.; Yamane, K.; Wang, W.; Bechhofer, D.H.
Bacillus subtilis RNase III cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor
J. Biol. Chem.
273
19542-19547
1998
Bacillus subtilis, Saccharomyces cerevisiae, Schizosaccharomyces pombe
Manually annotated by BRENDA team
Olmedo, G.; Guzman, P.
Mini-III, a fourth class of RNase III catalyses maturation of the Bacillus subtilis 23S ribosomal RNA
Mol. Microbiol.
68
1073-1076
2008
Bacillus subtilis
Manually annotated by BRENDA team
Redko, Y.; Bechhofer, D.H.; Condon, C.
Mini-III, an unusual member of the RNase III family of enzymes, catalyses 23S ribosomal RNA maturation in B. subtilis
Mol. Microbiol.
68
1096-1106
2008
Bacillus subtilis (O31418), Bacillus subtilis
Manually annotated by BRENDA team
Durand, S.; Gilet, L.; Condon, C.
The essential function of B. subtilis RNase III is to silence foreign toxin genes
PLoS Genet.
8
e1003181
2012
Bacillus subtilis
Manually annotated by BRENDA team