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Information on EC 3.1.26.11 - tRNase Z and Organism(s) Schizosaccharomyces pombe and UniProt Accession P87168

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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.26 Endoribonucleases producing 5'-phosphomonoesters
                3.1.26.11 tRNase Z
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This record set is specific for:
Schizosaccharomyces pombe
UNIPROT: P87168 not found.
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The taxonomic range for the selected organisms is: Schizosaccharomyces pombe
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
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endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule
Synonyms
elac2, trnase z, rnase z, rnase bn, trnase zl, 3' trnase, 3'-trnase, trnase zs, trna 3' processing endoribonuclease, rnase zs1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 tRNase
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3'-tRNA processing endoribonuclease
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nuclease, transfer ribonucleate maturation 3'-endoribo-(9CI)
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pre-tRNA processing endoribonuclease
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precursor tRNA 3'-end processing endoribonuclease
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transfer RNA maturation endonuclease
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tRNA 3 endonuclease
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tRNA 3' processing endoribonuclease
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tRNA precursor-processing endoribonuclease
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tRNase Z
tRNase ZL
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
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CAS REGISTRY NUMBER
COMMENTARY hide
98148-84-6
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
pre-tRNAarg + H2O
?
show the reaction diagram
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?
pre-tRNAArg + H2O
tRNAArg + 3'-leader of tRNA
show the reaction diagram
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?
pre-tRNALys + H2O
?
show the reaction diagram
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?
pre-tRNAVal + H2O
?
show the reaction diagram
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?
precursor tRNASer(UGA)-M + H2O
mature tRNASer(UGA)-M
show the reaction diagram
overexpression of wild-type trz1+ in yYH1 cells can cause an increase in nonsense suppression by tRNASer(UGA)-M1, but overexpression of trz1+ cannot overcome the requirement for Sla1p for maturation of tRNASer(UGA)-M
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?
additional information
?
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
trz2 overexpression is toxic to cells. It leads to apoptotic cell death. Overexpression of trz2 also caused a loss of mitochondrial membrane potential and an increased reactive oxygen species formation. Overexpressing trz2 increases the level of intracellular iron by about 2fold. trz2 overexpression may cause mitochondrial dysfunction, which is likely to lead to perturbation of iron homeostasis, ROS accumulation and induction of apoptotic cell death in Schizosaccharomyces pombe
physiological function
malfunction
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enzyme inactivation compromises nuclear and mitochondrial tRNA 3'-end processing
physiological function
additional information
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Schizosaccharomyces pombe contains two candidate tRNase ZLs encoded by the essential genes sptrz1+ and sptrz2+
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
75900
calculated from sequence
90600
calculated from sequence
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D476A
overexpression of Trz2 in yYH1 cells does not result in an increase in nonsense suppression by tRNASer(UGA)-M1
D578A
overexpression in yYH1 cells does not result in an increase in nonsense suppression by tRNASer(UGA)-M1
H574A
overexpression in yYH1 cells does not result in an increase in nonsense suppression by tRNASer(UGA)-M1
I572K
inactivates the protein
Y571L
mutation does not result in a temperature-sensitive phenotype
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
PCR products for trz2+ digested with XhoI and XmaI, and cloned into the XhoI and XmaI sites of pREP82X and pREP4X, producing pREP82X-f-trz2 and pREP4X-f-trz2, respectively. PCR products for the 5' and 3' flanks of trz2+ cloned into the SacI/PstI sites and SalI/KpnI sites of pAF1, creating pAF1-trz2int
functional expression of isozymes in Schizosaccharomyces pombe cells
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PCR products for trz1+ digested with SalI and XmaI, and cloned between the SalI/XmaI sites of pREP4X (the full strength thiamine-repressible nmt1+ promoter) and the XhoI/XmaI sites pREP82X (the lowstrength thiamine-repressible nmt1+ promoter), yielding pREP4X-f-trz1 and pREP82X-f-trz1, respectively. Overexpression of wild-type trz1+ and mutants in yYH1 cells or in an isogenetic sla1DELTA strain (yAS110)
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
trz2 is expressed at a very low level. The TATA-like element is essential for trz2 expression. The low-level trz2 expression may arise from a low level of transcription caused by lack of a strong TATA box and the negative regulatory element (NRE)
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Spaeth, B.; Canino, G.; Marchfelder, A.
tRNase Z: the end is not in sight
Cell. Mol. Life Sci.
64
2404-2412
2007
Arabidopsis thaliana, Caenorhabditis elegans, Danio rerio, Drosophila melanogaster (Q8MKW7), Gallus gallus, Homo sapiens, Mus musculus, Neurospora crassa, Saccharomyces cerevisiae, Schizosaccharomyces pombe
Manually annotated by BRENDA team
Zhao, Z.; Su, W.; Yuan, S.; Huang, Y.
Functional conservation of tRNase ZL among Saccharomyces cerevisiae, Schizosaccharomyces pombe and humans
Biochem. J.
422
483-492
2009
Homo sapiens, Saccharomyces cerevisiae, Schizosaccharomyces pombe (P87168), Schizosaccharomyces pombe (Q10155), Schizosaccharomyces pombe
Manually annotated by BRENDA team
Gan, X.; Yang, J.; Li, J.; Yu, H.; Dai, H.; Liu, J.; Huang, Y.
The fission yeast Schizosaccharomyces pombe has two distinct tRNase ZLs encoded by two different genes and differentially targeted to the nucleus and mitochondria
Biochem. J.
435
103-111
2011
Schizosaccharomyces pombe
Manually annotated by BRENDA team
Zhang, X.; Zhao, Q.; Huang, Y.
Partitioning of the nuclear and mitochondrial tRNA 3-end processing activities between two different proteins in Schizosaccharomyces pombe
J. Biol. Chem.
288
27415-27422
2013
Schizosaccharomyces pombe, Schizosaccharomyces pombe YHL6381
Manually annotated by BRENDA team
Shang, J.; Wu, L.; Yang, Y.; Li, Y.; Liu, Z.; Huang, Y.
Overexpression of Schizosaccharomyces pombe tRNA 3-end processing enzyme Trz2 leads to an increased cellular iron level and apoptotic cell death
Fungal Genet. Biol.
122
11-20
2019
Schizosaccharomyces pombe (P87168), Schizosaccharomyces pombe, Schizosaccharomyces pombe ATCC 24843 (P87168)
Manually annotated by BRENDA team
Liu, J.; Huang, L.; Wang, Y.; Huang, Y.
Characterization of cis-elements in the promoter of trz2 encoding Schizosaccharomyces pombe mitochondrial tRNA 3'-end processing enzyme
Microbiology
163
75-85
2017
Schizosaccharomyces pombe (P87168), Schizosaccharomyces pombe (Q10155), Schizosaccharomyces pombe, Schizosaccharomyces pombe ATCC 24843 (P87168), Schizosaccharomyces pombe ATCC 24843 (Q10155)
Manually annotated by BRENDA team