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Information on EC 3.1.21.B3 - mismatch-specific endonuclease

for references in articles please use BRENDA:EC3.1.21.B3
preliminary BRENDA-supplied EC number
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The expected taxonomic range for this enzyme is: Thermococcus kodakarensis
Reaction Schemes
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endonucleolytic cleavage of both strands of double-stranded DNA into 5'-protruding forms, with the mismatched base pair in the central position. The enzyme cleaves G/T, G/G, T/T, T/C and A/G mismatches, with a preference for G/T, G/G and T/T. It cleaves the third phosphodiester bond on the 5' side of the mismatched base in both strands, to produce a cohesive end with a five-nucleotide long 5'-protrusion, leaving 5'-phosphate and 3'-hydroxyl termini.
Synonyms
mismatch-specific endonuclease, endonuclease nucs, more
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
endonucleolytic cleavage of both strands of double-stranded DNA into 5'-protruding forms, with the mismatched base pair in the central position. The enzyme cleaves G/T, G/G, T/T, T/C and A/G mismatches, with a preference for G/T, G/G and T/T. It cleaves the third phosphodiester bond on the 5' side of the mismatched base in both strands, to produce a cohesive end with a five-nucleotide long 5'-protrusion, leaving 5'-phosphate and 3'-hydroxyl termini.
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