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Information on EC 3.1.21.1 - deoxyribonuclease I and Organism(s) Equus caballus and UniProt Accession Q4AEE3

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Equus caballus
UNIPROT: Q4AEE3 not found.
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Word Map
The taxonomic range for the selected organisms is: Equus caballus
The enzyme appears in selected viruses and cellular organisms
Synonyms
lactoferrin, dna polymerase, dnase i, colicin, diphtheria toxin, dnaase, deoxyribonuclease, dnasei, deoxyribonuclease i, crm197, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
deoxyribonuclease I
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deoxyribonuclease
-
-
-
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deoxyribonuclease A
-
-
-
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deoxyribonucleic phosphatase
-
-
-
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desoxyribonuclease
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-
-
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DNA endonuclease
-
-
-
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DNase
-
-
-
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DNase I
-
-
-
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Dornase alfa
-
-
-
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endodeoxyribonuclease I
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-
-
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Escherichia coli endonuclease I
-
-
-
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nuclease, deoxyribo-
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-
-
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nuclease, Escherichia coli endo-, I
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-
-
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pancreatic deoxyribonuclease
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-
-
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pancreatic DNase
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-
-
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streptodornase
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
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-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
9003-98-9
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
DNA + H2O
5'-phosphodinucleotide + 5'-phosphooligonucleotide
show the reaction diagram
-
-
-
?
salmon testis DNA + H2O
5'-phosphooligonucleotides + ?
show the reaction diagram
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
10 mM, complete loss of activity
EGTA
1 mM, complete loss of activity
Trypsin
is less resistant to trypsin than human DNase I, DNase I activity decreases gradually
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
poor activity
Manually annotated by BRENDA team
poor activity
Manually annotated by BRENDA team
poor activity
Manually annotated by BRENDA team
additional information
marked correlation between tissue distribution of enzyme and its sensitivity/resistance to proteolysis
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
DNAS1_HORSE
282
1
31708
Swiss-Prot
Secretory Pathway (Reliability: 3)
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
protein contains a N-linked carbohydrate moiety. Potential glycosylation sites at N18 and N106
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
N106Q
enzyme activity is lower than that of the wild-type, is unstable to heat and less resistant to trypsin
N18Q
enzyme activity is lower than that of the wild-type, is unstable to heat and less resistant to trypsin
N18Q/N106Q
enzyme activity is lower than those of the single mutants, is unstable to heat and is the most sensitive to trypsin
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
N18 and N106 are both necessary for full enzymatic activity, heat-stability, and trypsin resistance
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
from parotid gland
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in COS-7 cell
expression of wild-type and mutants in COS-7 cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ueki, M.; Takeshita, H.; Fujihara, J.; Ueta, G.; Nakajima, T.; Kominato, Y.; Kishi, K.; Iida, R.; Yasuda, T.
Susceptibility of mammalian deoxyribonucleases I (DNases I) to proteolysis by proteases and its relationships to tissue distribution: Biochemical and molecular analysis of equine DNase I
Comp. Biochem. Physiol. B
148B
93-102
2007
Equus caballus (Q4AEE3)
-
Manually annotated by BRENDA team
Fujihara, J.; Yasuda, T.; Kunito, T.; Fujii, Y.; Takatsuka, H.; Moritani, T; Takeshita, H.
Two N-linked glycosylation sites (Asn18 and Asn106) are both required for full enzymatic activity, thermal stability, and resistance to proteolysis in mammalian deoxyribonuclease I
Biosci. Biotechnol. Biochem.
72
3197-3205
2008
Bos taurus, Homo sapiens (P24855), Homo sapiens, Equus caballus (Q4AEE3), Equus caballus
Manually annotated by BRENDA team