Information on EC 3.1.2.7 - glutathione thiolesterase

for references in articles please use BRENDA:EC3.1.2.7
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.1.2.7
-
RECOMMENDED NAME
GeneOntology No.
glutathione thiolesterase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-acylglutathione + H2O = glutathione + a carboxylate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of thioester
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
S-acylglutathione hydrolase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9025-99-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-acetylglutathione + H2O
glutathione + acetate
show the reaction diagram
S-butyrylglutathione + H2O
glutathione + butanoate
show the reaction diagram
-
-
-
-
?
S-lactoylglutathione + H2O
glutathione + lactate
show the reaction diagram
-
-
-
?
S-propionylglutathione + H2O
glutathione + propionate
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
no substrate: acetyl-CoA, acetylthiocholine
-
-
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
-
8-hydroxyquinoline
-
-
iodoacetamide
-
-
N-ethylmaleimide
-
-
p-chloromercuribenzoate
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.2
S-acetylglutathione
-
pH 7.2
0.015
S-butyrylglutathione
-
pH 7.2
0.08
S-propionylglutathione
-
pH 7.2
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.15
-
-
0.369
-
-
9.1
-
pH 7.2
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 7.5
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 8
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
activity raises with temperature up to 60°C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28000
-
enzyme P3, gel filtration
32000
-
enzyme P2, gel filtration
56000
-
enzyme P1, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 28000, enzyme P3, 1 * 32000, enzyme P2, 1 * 56000, enzyme P1, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
-
stable for at least 30 min
100
-
5 min, complete inactivation
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE