Information on EC 3.1.2.25 - phenylacetyl-CoA hydrolase

for references in articles please use BRENDA:EC3.1.2.25
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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.1.2.25
-
RECOMMENDED NAME
GeneOntology No.
phenylacetyl-CoA hydrolase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
phenylglyoxylyl-CoA + H2O = phenylglyoxylate + CoA
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
phenylacetate degradation II (anaerobic)
-
-
Phenylalanine metabolism
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-
SYSTEMATIC NAME
IUBMB Comments
phenylglyoxylyl-CoA hydrolase
This is the second step in the conversion of phenylacetyl-CoA to phenylglyoxylate, the first step being carried out by EC 1.17.5.1, phenylacetyl-CoA dehydrogenase.
CAS REGISTRY NUMBER
COMMENTARY hide
210756-43-7
cf. 1.17.5.1
57219-72-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
; strain DSM 6984
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
show the reaction diagram
-
hydrolyzes p-nitrophenyl acetate at the same rate as phenylacetyl-CoA or phenoxyacetylglycine
-
?
phenoxyacetyl-CoA + H2O
phenoxyacetate + CoA
show the reaction diagram
-
approximately 5fold higher activity than with phenylacetyl-CoA
-
?
phenoxyacetylglycine + H2O
?
show the reaction diagram
-
hydrolyzes phenoxyacetylglycine at the same rate as phenylacetyl-CoA or p-nitrophenyl acetate
-
?
phenylacetyl-CoA + H2O
phenylacetate + CoA
show the reaction diagram
-
hydrolyzes phenylacetyl-CoA at the same rate as p-nitrophenyl acetate or phenoxyacetylglycine, approximately 5fold lower activity as with phenoxyacetyl-CoA
-
?
phenylglyoxylyl-CoA + H2O
phenylglyoxylate + CoA
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
phenylglyoxylyl-CoA + H2O
phenylglyoxylate + CoA
show the reaction diagram
-
involved in anaerobic metabolism of phenylalanine, catalyzes the second step in the conversion of phenylacetyl-CoA to phenylglyoxylate, the first step being carried out by EC 1.17.5.1
-
?
additional information
?
-
-
catalyzes the second step in the conversion of phenylacetyl-CoA to phenylglyoxylate, the first step being carried out by EC 1.17.5.1
-
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
sensitive to SH group inhibitors
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.95
phenylacetyl-CoA
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-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25000
-
gel filtration
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
thiols are necessary during purification to prevent oxidation
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653136
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE