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IUBMB Comments Acts on the acyl-carrier-protein thioester of C12 and, with a much lower activity, C14 fatty acids. The derivative of oleic acid is hydrolysed very slowly (cf . EC 3.1.2.14 , oleoyl-[acyl-carrier-protein] hydrolase).
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms 12-acp thioesterase, 12:0-acp te, lauroyl-acp thioesterase, lauroyl-acyl carrier protein thioesterase, more
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12-ACP thioesterase
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Dodecanoyl-acyl-carrier-protein hydrolase
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Dodecyl-acyl-carrier protein hydrolase
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Hydrolase, lauroyl-[acyl carrier protein]
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lauroyl-ACP thioesterase
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Lauroyl-acyl carrier protein thioesterase
Lauryl-acyl-carrier-protein hydrolase
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C12:0-ACP thioesterase
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C12:0-ACP thioesterase
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Lauroyl-acyl carrier protein thioesterase
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Lauroyl-acyl carrier protein thioesterase
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a dodecanoyl-[acyl-carrier protein] + H2O = an [acyl-carrier protein] + dodecanoate
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hydrolysis of thioester
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MetaCyc
palmitate biosynthesis I (type I fatty acid synthase), palmitate biosynthesis II (type II fatty acid synthase), tetradecanoate biosynthesis (mitochondria)
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dodecanoyl-[acyl-carrier protein] hydrolase
Acts on the acyl-carrier-protein thioester of C12 and, with a much lower activity, C14 fatty acids. The derivative of oleic acid is hydrolysed very slowly (cf. EC 3.1.2.14, oleoyl-[acyl-carrier-protein] hydrolase).
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decanoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + decanoate
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Substrates: - Products: -
?
dodecanoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + dodecanoate
hexadecanoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + hexadecanoate
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Substrates: - Products: -
?
lauroyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + laurate
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Substrates: - Products: -
?
Octadecenoyl-[acyl-carrier protein] + H2O
[Acyl-carrier protein] + octadecenoate
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Substrates: - Products: -
?
tetradecanoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + tetradecanoate
undecanoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + undecanoate
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Substrates: - Products: -
?
dodecanoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + dodecanoate
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Substrates: - Products: -
?
dodecanoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + dodecanoate
Substrates: - Products: -
?
dodecanoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + dodecanoate
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Substrates: the C-terminal two-third of the protein is critical for the specificity Products: -
?
dodecanoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + dodecanoate
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Substrates: extreme preference for dodecanoyl-[acyl-carrier protein] Products: -
?
dodecanoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + dodecanoate
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Substrates: enzyme plays a major role in medium-chain fatty acid production Products: -
?
tetradecanoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + tetradecanoate
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Substrates: - Products: -
?
tetradecanoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + tetradecanoate
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Substrates: weak activity Products: -
?
tetradecanoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + tetradecanoate
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Substrates: the C-terminal two-third of the protein is critical for the specificity Products: -
?
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dodecanoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + dodecanoate
lauroyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + laurate
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Substrates: - Products: -
?
dodecanoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + dodecanoate
Substrates: - Products: -
?
dodecanoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + dodecanoate
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Substrates: enzyme plays a major role in medium-chain fatty acid production Products: -
?
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NaCl
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optimal concentration: 20-40 mM
phosphate
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optimal concentration: 15-20 mM
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Ca2+
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1 mM, 50% inhibition
cholate
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slight stimulation at 0.1 and 0.01% w/v. Strong inhibition at 1% w/v
Cu2+
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1 mM, 100% inhibition
diethyldicarbonate
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1 mM, 97% inhibition
dodecanoyl-[acyl-carrier protein]
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competitive with respect to tetradecanoyl-[acyl-carrier protein], wild type enzyme and mutant enzyme M197R/R199H/T123K
Mg2+
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1 mM, 50% inhibition
Mn2+
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1 mM, 50% inhibition
NaCl
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100 mM, 50% inhibition, fully reversible by dialysis or dilution
NEM
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5 mM, complete inhibition
octylglucoside
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stimulation at 0.1 and 0.01% w/v. Strong inhibition at 1% w/v
phosphate
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100 mM, 50% inhibition, fully reversible by dialysis or dilution
Zn2+
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1 mM, 100% inhibition
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cholate
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slight stimulation at 0.1 and 0.01% w/v. Strong inhibition at 1% w/v
octylglucoside
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stimulation at 0.1 and 0.01% w/v. Strong inhibition at 1% w/v
Triton X-100
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stimulation
Zwittergent 3-8
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stimulation
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0.0023
decanoyl-[acyl-carrier protein]
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0.0019 - 0.0166
dodecanoyl-[acyl-carrier protein]
0.0036
hexadecanoyl-[acyl-carrier protein]
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0.01
octadecenoyl-[acyl-carrier protein]
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0.0023 - 0.0064
tetradecanoyl-[acyl-carrier protein]
0.0067
undecanoyl-[acyl-carrier protein]
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tetradecanoyl-[acyl-carrier protein]
0.0019
dodecanoyl-[acyl-carrier protein]
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wild type enzyme
0.002 - 0.003
dodecanoyl-[acyl-carrier protein]
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0.0166
dodecanoyl-[acyl-carrier protein]
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0.0023
tetradecanoyl-[acyl-carrier protein]
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triple mutant M197R/R199H/T231K
0.0064
tetradecanoyl-[acyl-carrier protein]
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wild type enzyme
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10
NaCl
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fully reversible by dialysis or dilution
100
phosphate
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fully reversible by dialysis or dilution
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brenda
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brenda
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UniProt
brenda
recombinant enzyme expressed in Brassica napus plants
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brenda
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immature
brenda
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of Brassica napus transformed with Umbellularia californica enzyme cDNA. The normally seed-localized enzyme is expressed in active form in leaves and imported into chloroplast
brenda
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brenda
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brenda
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of transformed Brassica napus leaves
brenda
Highest Expressing Human Cell Lines
Filter by:
Cell Line Links
Gene Links
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FATB_CINCA
382
0
42675
Swiss-Prot
Chloroplast (Reliability: 4 )
FATB_UMBCA
382
0
42915
Swiss-Prot
Chloroplast (Reliability: 3 )
FATB_UMBCA
382
0
42915
Swiss-Prot
Chloroplast (Reliability: 3 )
C0RMX3_BRUMB
Brucella melitensis biotype 2 (strain ATCC 23457)
646
7
68135
TrEMBL
-
A0A7W7DU04_9ACTN
255
0
27561
TrEMBL
-
C4WPS1_9HYPH
616
6
64394
TrEMBL
-
C0G3Y6_9HYPH
826
8
86095
TrEMBL
-
A0A7X5UPN0_9PSEU
245
0
26293
TrEMBL
-
A0A1E3L8J5_9BACL
251
0
28811
TrEMBL
-
A0A840VW06_9ACTN
253
0
27553
TrEMBL
-
A0A7W5Z7H1_9HYPH
239
0
26198
TrEMBL
-
A0A7X0M8S3_9ACTN
265
0
28671
TrEMBL
-
A0A7W9MJX0_9ACTN
270
0
29060
TrEMBL
-
A0A841H206_9BACT
259
0
28850
TrEMBL
-
A0A1E3L566_9BACL
241
0
27715
TrEMBL
-
C0G843_9HYPH
802
6
82813
TrEMBL
-
A0A7W0HTX1_9ACTN
248
0
27569
TrEMBL
-
C4WJY9_9HYPH
837
6
86567
TrEMBL
-
A0A7W9JSX6_9XANT
247
0
27414
TrEMBL
-
A0A853AI88_9PSEU
253
0
27632
TrEMBL
-
C4ITL0_BRUAO
808
6
83650
TrEMBL
-
A0A7W0HUV3_9ACTN
249
0
27591
TrEMBL
-
A0A7W9ADA3_9XANT
294
0
33185
TrEMBL
-
A0A853AIA1_9PSEU
238
0
25736
TrEMBL
-
A0A7W7PYT6_9ACTN
262
0
28128
TrEMBL
-
A0A853BJ27_9ACTN
261
0
27449
TrEMBL
-
A0A7W7WCN7_9ACTN
257
0
27799
TrEMBL
-
A0A7Y9D9B2_CLOBE
252
0
29904
TrEMBL
-
A0A7W7Q3I7_9PSEU
256
0
28201
TrEMBL
-
A0A841H2J6_9BACT
255
0
27762
TrEMBL
-
A0A7W9HA85_9ACTN
247
0
26918
TrEMBL
-
A0A7W9LQ81_9ACTN
266
0
28757
TrEMBL
-
A0A7W6PRF9_9HYPH
266
0
29622
TrEMBL
-
A0A5P2XGQ5_STRST
259
0
27988
TrEMBL
-
A0A852Z189_9ACTN
248
0
27253
TrEMBL
-
A0A852UMT2_9ACTN
295
0
31150
TrEMBL
-
A0A7W5BEP6_9BURK
239
0
26734
TrEMBL
-
A0A7W7VSV6_KITKI
248
0
26864
TrEMBL
-
C4IN82_BRUAO
759
8
79224
TrEMBL
-
A0A7W6H484_9HYPH
266
0
28016
TrEMBL
-
A0A840TWY0_9BACT
248
0
28316
TrEMBL
-
C4IP30_BRUAO
752
8
81042
TrEMBL
-
C0RH72_BRUMB
Brucella melitensis biotype 2 (strain ATCC 23457)
752
8
81053
TrEMBL
-
A0A7W4ZT42_9ACTN
257
0
28562
TrEMBL
-
A0A7W6L4X6_XANCA
247
0
27383
TrEMBL
-
A0A7X0EF25_9PROT
279
0
30306
TrEMBL
-
A0A7W5HIT5_9GAMM
265
0
30034
TrEMBL
-
A0A7X0AV38_9PROT
247
0
26539
TrEMBL
-
A0A8I0NZV9_9ACTN
268
0
29501
TrEMBL
-
A0A7W9HJU5_9PSEU
246
0
26934
TrEMBL
-
A0A7W7MPR2_9ACTN
253
0
27390
TrEMBL
-
A0A7W7SZR0_9ACTN
256
0
28409
TrEMBL
-
C0RFQ9_BRUMB
Brucella melitensis biotype 2 (strain ATCC 23457)
804
6
83177
TrEMBL
-
A0A840QK13_9PSEU
253
0
27887
TrEMBL
-
C0G8W2_9HYPH
606
5
64232
TrEMBL
-
C0RGR8_BRUMB
Brucella melitensis biotype 2 (strain ATCC 23457)
826
8
86027
TrEMBL
-
A0A839W2P1_9PROT
250
0
26867
TrEMBL
-
A0A7W9QAN3_9ACTN
251
0
27513
TrEMBL
-
A0A841JVE8_9BACT
244
0
27259
TrEMBL
-
A0A7X0AKA4_9GAMM
258
0
28969
TrEMBL
-
A0A7W7DTM8_9ACTN
266
0
29794
TrEMBL
-
A0A841BWJ7_9ACTN
258
0
27866
TrEMBL
-
A0A840QEZ7_9PSEU
237
0
25965
TrEMBL
-
A0A840RWM5_9BURK
248
0
27525
TrEMBL
-
C0G4B1_9HYPH
752
8
81156
TrEMBL
-
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34000
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1 * 34000, SDS-PAGE
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monomer
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1 * 34000, SDS-PAGE
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M197E/E199H
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the double mutant M197R/R199H has equal preference for dodecanoyl-[acyl-carrier protein] and tetradecanoyl-[acyl-carrier protein]. Mutant T231K shows no altered specificity. Mutant R327Q and R322M/R327Q have specific activities towards dodecanoyl-[acyl-carrier protein] and tetradecanoyl-[acyl-carrier protein] decreased about 100fold and 30fold. The triple mutant M197R/R199H/T231K is a tetradecanoyl-[acyl-carrier protein] thioesterase
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stable to repeated thawings
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0-4°C, 20% v/v glycerol or ethylene glycol, approximately 50% of the enzyme activity remains after 24 h
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expressed in Nicotiana benthamiana leaf
expression in Brassica napus
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expression in Escherichia coli, and in developing oilseeds of Arabidopsis thaliana and Brassica napus
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Pollard, M.R.; Anderson, L.; Fan, C.; Hawkins, D.J.; Davies, H.M.
A specific acyl-ACP thioesterase implicated in medium-chain fatty acid production in immature cotyledons of Umbellularia californica
Arch. Biochem. Biophys.
284
306-312
1991
Umbellularia californica
brenda
Davies, H.M.; Anderson, L.; Fan, C.; Hawkins, D.J.
Developmental induction, purification, and further characterization of 12:0-ACP thioesterase from immature cotyledons of Umbellularia californica
Arch. Biochem. Biophys.
290
37-45
1991
Umbellularia californica
brenda
Yuan, L.; Voelker, T.A.; Hawkins, D.J.
Modification of the substrate specificity of an acyl-acyl carrier protein thioesterase by protein engineering
Proc. Natl. Acad. Sci. USA
92
10639-10643
1995
Umbellularia californica
brenda
Voelker, T.
Plant acyl-ACP thioesterase: chain-length determining enzymes in plant fatty acid biosynthesis
Genet. Eng. (N.Y.)
18
111-133
1996
Umbellularia californica
brenda
Eccleston, V.S.; Cranmer, A.M.; Voelker, T.A.; Ohlrogge, J.B.
Medium-chain fatty acid biosynthesis and utilization in Brassica napus: plant expressing lauroyl-acyl carrier protein thioesterase
Planta
198
46-53
1996
Umbellularia californica
-
brenda
Work, V.H.; Melnicki, M.R.; Hill, E.A.; Davies, F.K.; Kucek, L.A.; Beliaev, A.S.; Posewitz, M.C.
Lauric acid production in a glycogen-less strain of Synechococcus sp. PCC 7002
Front. Bioeng. Biotechnol.
3
48
2015
Synechococcus sp. PCC 7002
brenda
Reynolds, K.B.; Taylor, M.C.; Zhou, X.R.; Vanhercke, T.; Wood, C.C.; Blanchard, C.L.; Singh, S.P.; Petrie, J.R.
Metabolic engineering of medium-chain fatty acid biosynthesis in Nicotiana benthamiana plant leaf lipids
Front. Plant Sci.
6
164
2015
Umbellularia californica (Q41635)
brenda
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