Information on EC 3.1.2.17 - (S)-methylmalonyl-CoA hydrolase

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The expected taxonomic range for this enzyme is: Rattus norvegicus

EC NUMBER
COMMENTARY hide
3.1.2.17
-
RECOMMENDED NAME
GeneOntology No.
(S)-methylmalonyl-CoA hydrolase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(S)-methylmalonyl-CoA + H2O = methylmalonate + CoA
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of thioester
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-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Valine, leucine and isoleucine degradation
-
-
Propanoate metabolism
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-
SYSTEMATIC NAME
IUBMB Comments
(S)-methylmalonyl-CoA hydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
87928-03-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(S)-methylmalonyl-CoA + H2O
methylmalonate + CoA
show the reaction diagram
malonyl-CoA + H2O
malonate + CoA
show the reaction diagram
propionyl-CoA + H2O
propionate + CoA
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(S)-methylmalonyl-CoA + H2O
methylmalonate + CoA
show the reaction diagram
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
no cofactors identified
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
acetyl-CoA
-
competitive inhibition
butyryl-CoA
-
competitive
CoA
-
competitive inhibition
decanoyl-CoA
-
noncompetitive inhibition
glutaryl-CoA
-
competitive inhibition
heptanoyl-CoA
-
competitive inhibition
Hexanoyl-CoA
-
competitive inhibition
malonyl-CoA
-
competitive inhibition
nonanoyl-CoA
-
noncompetitive inhibition
Octanoyl-CoA
-
noncompetitive inhibition
palmitoyl-CoA
-
noncompetitive inhibition
pentanoyl-CoA
-
competitive inhibition
propionyl-CoA
-
competitive inhibition
succinyl-CoA
-
competitive inhibition
Tridecanoyl-CoA
-
noncompetitive inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.7
(S)-methylmalonyl-CoA
-
pH 6.0, 30°C
14
acetyl-CoA
-
pH 6.0, 30°C
1.4
DL-methylmalonyl-CoA
-
pH 6.0, 30°C
0.7
malonyl-CoA
-
pH 6.0, 30°C
1.9
propionyl-CoA
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pH 6.0, 30°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
23.3
(S)-methylmalonyl-CoA
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pH 6.0, 30°C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.1
acetyl-CoA
-
pH 6.0, 30°C
2.6
butyryl-CoA
-
pH 6.0, 30°C
1.4
CoA
-
pH 6.0, 30°C
0.05
decanoyl-CoA
-
pH 6.0, 30°C
4.9
glutaryl-CoA
-
pH 6.0, 30°C
1.5
heptanoyl-CoA
-
pH 6.0, 30°C
1.7
Hexanoyl-CoA
-
pH 6.0, 30°C
0.6
malonyl-CoA
-
pH 6.0, 30°C
0.2
nonanoyl-CoA
-
pH 6.0, 30°C
0.5
Octanoyl-CoA
-
pH 6.0, 30°C
0.01
palmitoyl-CoA
-
pH 6.0, 30°C
3
propionyl-CoA
-
pH 6.0, 30°C
1.5
succinyl-CoA
-
pH 6.0, 30°C
0.04
Tridecanoyl-CoA
-
pH 6.0, 30°C
2.6
valeryl-CoA
-
pH 6.0, 30°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
-
50% activity at pH 5.3 and pH 7.1
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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1 * 35000, SDS-PAGE, in absence and presence of 1% 2-mercaptoethanol
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, purified enzyme stable for at least 1 month
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE