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double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
oligonucleotides + H2O
5'-phosphomononucleotides
single-stranded DNA + H2O
5'-phosphomononucleotides
additional information
?
-
double-stranded DNA + H2O

single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
-
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
degradation also at single-stranded breaks within DNA-duplex
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
degrades double-stranded native DNA
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
-
133978, 133979, 133980, 133981, 133983, 133984, 133985, 133986, 133987, 133988, 133989, 133992 -
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
exonucleolytic cleavage in the 5'- to 3'-direction to yield 5'-phosphomononucleotides
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
degradation also at single-stranded breaks within DNA-duplex
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
possess "red" recombination system
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
high processivity
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
no activity on denatured DNA or RNA
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
degrades native DNA 100 times faster than long single-stranded DNA or oligonucleotides
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
shows strong preference for termini bearing 5'-phosphoryl groups
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
no attack at single-stranded breaks within DNA-duplex
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
degrades double-stranded native DNA
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
preference for a 5'-phosphoryl group over a 5'-hydroxyl group
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
lambda exonuclease is unable to initiate DNA digestion at nicks or gaps
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
-
133978, 133979, 133980, 133983, 133984, 133985, 133986, 133987, 133988, 133989, 133992 -
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
exonucleolytic cleavage in the 5'- to 3'-direction to yield 5'-phosphomononucleotides
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
possess "red" recombination system
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
high processivity
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
no activity on denatured DNA or RNA
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
degrades native DNA 100 times faster than long single-stranded DNA or oligonucleotides
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
shows strong preference for termini bearing 5'-phosphoryl groups
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
no attack at single-stranded breaks within DNA-duplex
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
degrades double-stranded native DNA
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
preference for a 5'-phosphoryl group over a 5'-hydroxyl group
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
-
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
-
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
no activity on denatured DNA or RNA
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
degrades double-stranded native DNA
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
-
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
activity depends on lenght of double-stranded DNA
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
high processivity
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
degrades double-stranded native DNA
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
lambda exonuclease selectively digests the phosphorylated undesired strand of double-stranded DNA and produces single-stranded DNA in high yield and purity
-
-
?
double-stranded DNA + H2O
single-stranded DNA + 5'-phospho-2'-deoxynucleotides
-
sequence-dependent rate and pausing of individual lambda exonuclease enzymes during the digestion of a DNA sequence containing 120 CGG repeats. GC-rich repetitive sequences provide a significant barrier to lambda exonuclease activity by increasing the probability of pausing in addition to reducing the average digestion rate
-
-
?
oligonucleotides + H2O

5'-phosphomononucleotides
-
strong preference for relatively short chain oligonucleotides
-
-
-
oligonucleotides + H2O
5'-phosphomononucleotides
-
low rate
-
-
-
oligonucleotides + H2O
5'-phosphomononucleotides
-
strong preference for relatively short chain oligonucleotides
-
-
-
oligonucleotides + H2O
5'-phosphomononucleotides
-
low rate
-
-
-
single-stranded DNA + H2O

5'-phosphomononucleotides
-
-
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
-
mechanism for stimulation of recombination
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
-
degrades the 5'-terminated strand at a single-stranded branch
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
-
rate of digestion and binding is inversely related to the chain lenght
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
-
digestion not processively
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
-
at single-stranded DNA branches
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
-
-
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
-
mechanism for stimulation of recombination
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
-
degrades the 5'-terminated strand at a single-stranded branch
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
-
rate of digestion and binding is inversely related to the chain lenght
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
-
digestion not processively
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
-
binds to circular or linear single-stranded DNA
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
-
at single-stranded DNA branches
-
-
?
single-stranded DNA + H2O
5'-phosphomononucleotides
-
degrades ssDNA at higher enzyme concentrations
-
-
?
additional information

?
-
-
for enzyme digestion of single DNA molecules, individual lambda-DNA molecules labeled with the fluorescent dye, YOYO-1, are stretched in a laminar flow stream and immobilized on a bare fused-silica prism surface based on hydrophobic and electrostatic interactions. Enzyme digestion is initiated by the influx of lambda-exonuclease enzyme via capillary force, method evaluation, overview. Progression of single-DNA digestion by lambda-exonuclease enzyme based on the decrease in the length of the DNA molecule at a dye:bp ratio of 1:5
-
-
-
additional information
?
-
-
the enzyme unwinds the DNA prior to cleavage, such that two nucleotides of the 5'-ended strand insert into the active site of one subunit of the trimer, while the 3'-ended strand passes through the central channel to emerge out the back of the trimer. Unwinding of the DNA is facilitated by several apolar residues, including Leu78, that wedge into the base pairs at the single/double-strand junction to form favorable hydrophobic interactions. The terminal 5' phosphate of the DNA binds to a positively charged pocket buried at the end of the active site, while the scissile phosphate bridges two active site Mg2+ ions, mechanism of progressivity, structure-activity analysis, overview
-
-
-
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Pierce, J.R.; Nazimiec, M.; Tang, M.s.
Comparison of sequence preference of tomaymycin- and anthramycin-DNA bonding by exonuclease III and lambda exonuclease digestion and UvrABC nuclease incision analysis
Biochemistry
32
7069-7078
1993
Escherichia coli, Escherichia virus Lambda
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Lehman, I.R.
Bacterial deoxyribonucleases
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
4
251-270
1971
Escherichia coli, Escherichia virus Lambda
-
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Mitsis, P.G.; Kwagh, J.G.
Characterization of the interaction of lambda exonuclease with the ends of DNA
Nucleic Acids Res.
27
3057-3063
1999
Escherichia coli, Escherichia virus Lambda
brenda
Little, J.W.
Lambda exonuclease
Gene Amplif. Anal.
2
135-145
1981
Escherichia coli, Escherichia virus Lambda
brenda
Kerr, C.; Sadowski, P.D.
Gene 6 exonuclease of bacteriophage T7. I. Purification and properties of the enzyme
J. Biol. Chem.
247
305-310
1972
Enterobacteria phage T7, Escherichia coli
brenda
Lindahl, T.
An exonuclease specific for double-stranded DNA: deoxyribonuclease from rabbit tissues
Methods Enzymol.
21
148-153
1971
Oryctolagus cuniculus
-
brenda
Sriprakash, K.S.; Lundh, N.; Huh, M.M.O.; Radding, C.M.
The specificity of lambda exonuclease. Interactions with single-atranded DNA
J. Biol. Chem.
250
5438-5445
1975
Escherichia coli, Escherichia virus Lambda
brenda
Cassuto, E.; Radding, C.M.
Mechanism for the action of lambda exonuclease in genetic recombination
Nature New Biol.
229
13-16
1971
Escherichia coli, Escherichia virus Lambda
brenda
Radding, C.M.
The role of exonuclease and beta protein of bacteriophage lambda in genetic recombination. I. Effects of red mutants on protein structure
J. Mol. Biol.
52
491-499
1970
Escherichia coli, Escherichia virus Lambda
brenda
Little, J.W.; Lehman, I.R.; Kaiser, A.D.
An exonuclease induced by bacteriophage lambda. I. Preparation of the crystalline enzyme
J. Biol. Chem.
242
672-678
1967
Escherichia coli, Escherichia virus Lambda
brenda
Little, J.W.
An exonuclease induced by bacteriophage lambda. II. Nature of the enzymatic reaction
J. Biol. Chem.
242
679-686
1967
Escherichia coli, Escherichia virus Lambda
brenda
Radding, C.M.; Szpirer, J.; Thomas, R.
The structural gene for lambda exonuclease
Proc. Natl. Acad. Sci. USA
57
277-283
1967
Escherichia coli, Escherichia virus Lambda, Escherichia virus Lambda phage 80
brenda
Carter, D.M.; Radding, C.M.
The role of exonuclease and beta protein of phage lambda in genetic recombination. II. Substrate specificity and the mode of action of lambda exonuclease
J. Biol. Chem.
246
2502-2512
1971
Escherichia coli, Escherichia virus Lambda
brenda
Dolberg, M.; Baur, C.P.; Knippers, R.
Purification and characterization of a novel 5' exodeoxyribonuclease from the yeast Saccharomyces cerevisiae
Eur. J. Biochem.
198
783-787
1991
Saccharomyces cerevisiae
brenda
Rudd, E.A.
Reversible inhibition of lambda exonuclease with high pressure
Biochem. Biophys. Res. Commun.
230
140-142
1997
Escherichia coli, Escherichia virus Lambda
brenda
Kovall, R.A.; Matthews, B.W.
Structural, functional, and evolutionary relationships between lambda-exonuclease and the type II restriction endonucleases
Proc. Natl. Acad. Sci. USA
95
7893-7897
1998
Escherichia coli, Escherichia virus Lambda
brenda
Kim, S.; Blainey, P.C.; Schroeder, C.M.; Xie, X.S.
Multiplexed single-molecule assay for enzymatic activity on flow-stretched DNA
Nat. Methods
4
397-399
2007
Escherichia virus Lambda (P03697)
brenda
Balagurumoorthy, P.; Adelstein, S.J.; Kassis, A.I.
Method to eliminate linear DNA from mixture containing nicked circular, supercoiled, and linear plasmid DNA
Anal. Biochem.
381
172-174
2008
Escherichia coli
brenda
Song, C.; Zhao, M.
Real-time monitoring of the activity and kinetics of T4 polynucleotide kinase by a singly labeled DNA-hairpin smart probe coupled with lambda exonuclease cleavage
Anal. Chem.
81
1383-1388
2009
Escherichia coli
brenda
Conroy, R.S.; Koretsky, A.P.; Moreland, J.
Lambda exonuclease digestion of CGG trinucleotide repeats
Eur. Biophys. J.
39
337-343
2010
synthetic construct
brenda
Avci-Adali, M.; Paul, A.; Wilhelm, N.; Ziemer, G.; Wendel, H.P.
Upgrading SELEX technology by using lambda exonuclease digestion for single-stranded DNA generation
Molecules
15
1-11
2010
synthetic construct
brenda
Kang, S.H.; Lee, S.; Yeung, E.S.
Digestion of individual DNA molecules by lambda-exonuclease at liquid-solid interface
Analyst
135
1759-1764
2010
Escherichia virus Lambda
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Zhang, J.; McCabe, K.A.; Bell, C.E.
Crystal structures of lambda exonuclease in complex with DNA suggest an electrostatic ratchet mechanism for processivity
Proc. Natl. Acad. Sci. USA
108
11872-11877
2011
Escherichia virus Lambda
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Yuan, Z.; Zhou, Y.; Gao, S.; Cheng, Y.; Li, Z.
Homogeneous and sensitive detection of microRNA with ligase chain reaction and lambda exonuclease-assisted cationic conjugated polymer biosensing
ACS Appl. Mater. Interfaces
6
6181-6185
2014
Escherichia virus Lambda
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Oliver-Calixte, N.J.; Uba, F.I.; Battle, K.N.; Weerakoon-Ratnayake, K.M.; Soper, S.A.
Immobilization of lambda exonuclease onto polymer micropillar arrays for the solid-phase digestion of dsDNAs
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86
4447-4454
2014
Escherichia virus Lambda
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