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Information on EC 3.1.1.93 - mycophenolic acid acyl-glucuronide esterase and Organism(s) Homo sapiens and UniProt Accession Q9NUJ1

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     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.93 mycophenolic acid acyl-glucuronide esterase
IUBMB Comments
This liver enzyme deglucuronidates mycophenolic acid O-acyl-glucuronide, a metabolite of the immunosuppressant drug mycophenolate that is thought to be immunotoxic.
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Select one or more organisms in this record:
This record set is specific for:
Homo sapiens
UNIPROT: Q9NUJ1
Word Map
The expected taxonomic range for this enzyme is: Homo sapiens
The taxonomic range for the selected organisms is: Homo sapiens
Synonyms
ABHD10, AcMPAG deglucuronidase, mycophenolic acid acyl-glucuronide deglucuronidase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ABHD10
AcMPAG deglucuronidase
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-
-
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mycophenolic acid acyl-glucuronide deglucuronidase
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SYSTEMATIC NAME
IUBMB Comments
mycophenolic acid O-acyl-glucuronide-ester hydrolase
This liver enzyme deglucuronidates mycophenolic acid O-acyl-glucuronide, a metabolite of the immunosuppressant drug mycophenolate that is thought to be immunotoxic.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
mycophenolic acid O-acyl-glucuronide + H2O
mycophenolic acid + D-glucuronate
show the reaction diagram
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-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
AgNO3
-
inhibition
CuCl2
-
inhibition
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5,5'-dithiobis(2-nitrobenzoic acid)
the recombinant enzyme is completely inhibited at 1 mM
AgNO3
the recombinant enzyme is completely inhibited at 0.1 mM
bis-4-nitrophenylphosphate
the recombinant enzyme shows about 20% residual activity at 1 mM
CdCl2
the recombinant enzyme shows about 20% residual activity at 1 mM
CuCl2
the recombinant enzyme shows about 20% residual activity at 1 mM
diisopropyl fluorophosphate
the recombinant enzyme shows about 63% residual activity at 1 mM
Disulfiram
the recombinant enzyme shows about 23% residual activity at 1 mM
eserine
the recombinant enzyme shows about 85% residual activity at 1 mM
N-ethylmaleimide
the recombinant enzyme shows about 75% residual activity at 1 mM
phenylmethylsulfonyl fluoride
the recombinant enzyme shows about 25% residual activity at 1 mM
bis-p-nitrophenylphosphate
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Disulfiram
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DTNB
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phenylmethylsulfonyl fluoride
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additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.1007
mycophenolic acid O-acyl-glucuronide
recombinant enzyme, in 100 mM Tris-HCl (pH 7.4), at 37C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00017
unpurified enzyme from cytosol, in 100 mM Tris-HCl (pH 7.4), at 37C
0.00954
after 55.1fold purification, in 100 mM Tris-HCl (pH 7.4), at 37C
7400
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pH 7.4, 37C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
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assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
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probenecid acyl glucuronide (PRAG) deglucuronidation catalyzed by ABHD10 suppressively regulates probenecid acyl glucuronide (PRAG) formation via multiple UGT enzymes in human liver
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
Sequence
ABHDA_HUMAN
306
0
33933
Swiss-Prot
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28000
x * 28000, precursor protein, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 28000, precursor protein, SDS-PAGE
PURIFICATION/commentary
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, CM Sepharose column chromatography, Mono P column chromatography, and Superdex 200 gel filtration
CLONED/commentary
ORGANISM
UNIPROT
LITERATURE
expressed in Sf9 insect cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Iwamura, A.; Fukami, T.; Higuchi, R.; Nakajima, M.; Yokoi, T.
Human alpha/beta hydrolase domain containing 10 (ABHD10) is responsible enzyme for deglucuronidation of mycophenolic acid acyl-glucuronide in liver
J. Biol. Chem.
287
9240-9249
2012
Homo sapiens, Homo sapiens (Q9NUJ1)
Manually annotated by BRENDA team
Ito, Y.; Fukami, T.; Yokoi, T.; Nakajima, M.
An Orphan esterase ABHD10 modulates probenecid acyl glucuronidation in human liver
Drug Metab. Dispos.
42
2109-2116
2014
Homo sapiens
Manually annotated by BRENDA team
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