Information on EC 3.1.1.93 - mycophenolic acid acyl-glucuronide esterase

for references in articles please use BRENDA:EC3.1.1.93
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The expected taxonomic range for this enzyme is: Homo sapiens

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EC NUMBER
COMMENTARY hide
3.1.1.93
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RECOMMENDED NAME
GeneOntology No.
mycophenolic acid acyl-glucuronide esterase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
mycophenolic acid O-acyl-glucuronide + H2O = mycophenolate + D-glucuronate
show the reaction diagram
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-
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-
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SYSTEMATIC NAME
IUBMB Comments
mycophenolic acid O-acyl-glucuronide-ester hydrolase
This liver enzyme deglucuronidates mycophenolic acid O-acyl-glucuronide, a metabolite of the immunosuppressant drug mycophenolate that is thought to be immunotoxic.
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
-
probenecid acyl glucuronide (PRAG) deglucuronidation catalyzed by ABHD10 suppressively regulates probenecid acyl glucuronide (PRAG) formation via multiple UGT enzymes in human liver
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
mycophenolic acid O-acyl-glucuronide + H2O
mycophenolic acid + D-glucuronate
show the reaction diagram
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-
-
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
AgNO3
-
inhibition
CuCl2
-
inhibition
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5,5'-dithiobis(2-nitrobenzoic acid)
the recombinant enzyme is completely inhibited at 1 mM
AgNO3
the recombinant enzyme is completely inhibited at 0.1 mM
bis-4-nitrophenylphosphate
the recombinant enzyme shows about 20% residual activity at 1 mM
bis-p-nitrophenylphosphate
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-
CdCl2
the recombinant enzyme shows about 20% residual activity at 1 mM
CuCl2
the recombinant enzyme shows about 20% residual activity at 1 mM
diisopropyl fluorophosphate
the recombinant enzyme shows about 63% residual activity at 1 mM
Disulfiram
DTNB
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eserine
the recombinant enzyme shows about 85% residual activity at 1 mM
N-ethylmaleimide
the recombinant enzyme shows about 75% residual activity at 1 mM
phenylmethylsulfonyl fluoride
additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.1007
mycophenolic acid O-acyl-glucuronide
recombinant enzyme, in 100 mM Tris-HCl (pH 7.4), at 37°C
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00017
unpurified enzyme from cytosol, in 100 mM Tris-HCl (pH 7.4), at 37°C
0.00954
after 55.1fold purification, in 100 mM Tris-HCl (pH 7.4), at 37°C
7400
-
pH 7.4, 37°C
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
-
assay at
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28000
x * 28000, precursor protein, SDS-PAGE
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 28000, precursor protein, SDS-PAGE
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, CM Sepharose column chromatography, Mono P column chromatography, and Superdex 200 gel filtration
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Sf9 insect cells
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LINKS TO OTHER DATABASES (specific for EC-Number 3.1.1.93)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)