Information on EC 3.1.1.93 - mycophenolic acid acyl-glucuronide esterase

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The expected taxonomic range for this enzyme is: Homo sapiens

EC NUMBER
COMMENTARY hide
3.1.1.93
-
RECOMMENDED NAME
GeneOntology No.
mycophenolic acid acyl-glucuronide esterase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
mycophenolic acid O-acyl-glucuronide + H2O = mycophenolate + D-glucuronate
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
mycophenolic acid O-acyl-glucuronide-ester hydrolase
This liver enzyme deglucuronidates mycophenolic acid O-acyl-glucuronide, a metabolite of the immunosuppressant drug mycophenolate that is thought to be immunotoxic.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
-
probenecid acyl glucuronide (PRAG) deglucuronidation catalyzed by ABHD10 suppressively regulates probenecid acyl glucuronide (PRAG) formation via multiple UGT enzymes in human liver
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
mycophenolic acid O-acyl-glucuronide + H2O
mycophenolic acid + D-glucuronate
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
AgNO3
-
inhibition
CuCl2
-
inhibition
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5,5'-dithiobis(2-nitrobenzoic acid)
the recombinant enzyme is completely inhibited at 1 mM
AgNO3
the recombinant enzyme is completely inhibited at 0.1 mM
bis-4-nitrophenylphosphate
the recombinant enzyme shows about 20% residual activity at 1 mM
bis-p-nitrophenylphosphate
-
-
CdCl2
the recombinant enzyme shows about 20% residual activity at 1 mM
CuCl2
the recombinant enzyme shows about 20% residual activity at 1 mM
diisopropyl fluorophosphate
the recombinant enzyme shows about 63% residual activity at 1 mM
Disulfiram
DTNB
-
-
eserine
the recombinant enzyme shows about 85% residual activity at 1 mM
N-ethylmaleimide
the recombinant enzyme shows about 75% residual activity at 1 mM
phenylmethylsulfonyl fluoride
additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.1007
mycophenolic acid O-acyl-glucuronide
recombinant enzyme, in 100 mM Tris-HCl (pH 7.4), at 37°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00017
unpurified enzyme from cytosol, in 100 mM Tris-HCl (pH 7.4), at 37°C
0.00954
after 55.1fold purification, in 100 mM Tris-HCl (pH 7.4), at 37°C
7400
-
pH 7.4, 37°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28000
x * 28000, precursor protein, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 28000, precursor protein, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, CM Sepharose column chromatography, Mono P column chromatography, and Superdex 200 gel filtration
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Sf9 insect cells