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EC Tree
IUBMB Comments The enzyme catalyses the ring opening of epsilon-lactones which are formed during degradation of dihydrocarveol by the Gram-positive bacterium Rhodococcus erythropolis DCL14. The enzyme also acts on ethyl caproate, indicating that it is an esterase with a preference for lactones (internal cyclic esters). The enzyme is not stereoselective.
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
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mlhB
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4-isopropenyl-7-methyloxepan-2-one + H2O = 6-hydroxy-3-isopropenylheptanoate
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7-isopropyl-4-methyloxepan-2-one + H2O = 6-hydroxy-3,7-dimethyloctanoate
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isoprop(en)ylmethyloxepan-2-one + H2O = 6-hydroxyisoprop(en)ylmethylhexanoate
general reaction
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isoprop(en)ylmethyloxepan-2-one lactonohydrolase
The enzyme catalyses the ring opening of epsilon-lactones which are formed during degradation of dihydrocarveol by the Gram-positive bacterium Rhodococcus erythropolis DCL14. The enzyme also acts on ethyl caproate, indicating that it is an esterase with a preference for lactones (internal cyclic esters). The enzyme is not stereoselective.
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(4R)-4-isopropenyl-7-methyl-2-oxo-oxepanone + H2O
6-hydroxy-3-(1-methylethenyl)heptanoic acid
(6R)-6-isopropenyl-3-methyl-2-oxo-oxepanone + H2O
5-(hydroxymethyl)-2,6-dimethylhept-6-enoic acid
epsilon-caprolactone + H2O
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additional information
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(4R)-4-isopropenyl-7-methyl-2-oxo-oxepanone + H2O
6-hydroxy-3-(1-methylethenyl)heptanoic acid
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(4R)-4-isopropenyl-7-methyl-2-oxo-oxepanone + H2O
6-hydroxy-3-(1-methylethenyl)heptanoic acid
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(6R)-6-isopropenyl-3-methyl-2-oxo-oxepanone + H2O
5-(hydroxymethyl)-2,6-dimethylhept-6-enoic acid
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(6R)-6-isopropenyl-3-methyl-2-oxo-oxepanone + H2O
5-(hydroxymethyl)-2,6-dimethylhept-6-enoic acid
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epsilon-caprolactone + H2O
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epsilon-caprolactone + H2O
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ethyl caproate + H2O
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23% of the activity with epsilon-caprolactone
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ethyl caproate + H2O
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23% of the activity with epsilon-caprolactone
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DCL14, catalyzing the ring opening of lactones which are formed during degradation of several monocyclic monoterpenes, including carvone and menthol
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DCL14, catalyzing the ring opening of lactones which are formed during degradation of several monocyclic monoterpenes, including carvone and menthol
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additional information
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additional information
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DCL14, catalyzing the ring opening of lactones which are formed during degradation of several monocyclic monoterpenes, including carvone and menthol
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additional information
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DCL14, catalyzing the ring opening of lactones which are formed during degradation of several monocyclic monoterpenes, including carvone and menthol
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2,2'-dipyridyl
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1 mM, 55% inhibition
2-Bromo-4'-nitroacetophenone
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1 mM, 84% inhibition
CaCl2
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1 mM, 27% inhibition
CoCl2
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1 mM, 71% inhibition
EDTA
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10 mM, 16% inhibition
HgCl2
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0.1 mM 95% inhibition
MnCl2
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1 mM, 37% inhibition
p-chloromercuribenzoate
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0.1 mM, 26% inhibition
phenylhydrazine
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1 mM, 17% inhibition
SDS
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1 mM, 78% inhibition
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7.5 - 10.5
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pH 7.5: about 60% of maximal activity, pH 10.5: about 50% of maximal activity
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13 - 37
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13°C: about 35% of maximal activity, 37°C: 75% of maximal activity
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monomer
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1 * 35000, gel filtration
monomer
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1 * 35000, gel filtration
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-20°C, MLH can be stored for 6 months, without loss of activity
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van der Vlugt-Bergmans, C.J.; van der Werf, M.J.
Genetic and biochemical characterization of a novel monoterpene epsilon-lactone hydrolase from Rhodococcus erythropolis DCL14
Appl. Environ. Microbiol.
67
733-741
2001
Rhodococcus erythropolis, Rhodococcus erythropolis DCL14
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3.1.1.83 monoterpene epsilon-lactone hydrolase
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