Information on EC 3.1.1.83 - monoterpene epsilon-lactone hydrolase

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The expected taxonomic range for this enzyme is: Rhodococcus erythropolis

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EC NUMBER
COMMENTARY hide
3.1.1.83
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RECOMMENDED NAME
GeneOntology No.
monoterpene epsilon-lactone hydrolase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
4-isopropenyl-7-methyloxepan-2-one + H2O = 6-hydroxy-3-isopropenylheptanoate
show the reaction diagram
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7-isopropyl-4-methyloxepan-2-one + H2O = 6-hydroxy-3,7-dimethyloctanoate
show the reaction diagram
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isoprop(en)ylmethyloxepan-2-one + H2O = 6-hydroxyisoprop(en)ylmethylhexanoate
show the reaction diagram
general reaction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
(4R)-carveol and (4R)-dihydrocarveol degradation
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(4S)-carveol and (4S)-dihydrocarveol degradation
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Limonene and pinene degradation
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Caprolactam degradation
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SYSTEMATIC NAME
IUBMB Comments
isoprop(en)ylmethyloxepan-2-one lactonohydrolase
The enzyme catalyses the ring opening of epsilon-lactones which are formed during degradation of dihydrocarveol by the Gram-positive bacterium Rhodococcus erythropolis DCL14. The enzyme also acts on ethyl caproate, indicating that it is an esterase with a preference for lactones (internal cyclic esters). The enzyme is not stereoselective.
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CAS REGISTRY NUMBER
COMMENTARY hide
335630-13-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(4R)-4-isopropenyl-7-methyl-2-oxo-oxepanone + H2O
6-hydroxy-3-(1-methylethenyl)heptanoic acid
show the reaction diagram
(6R)-6-isopropenyl-3-methyl-2-oxo-oxepanone + H2O
5-(hydroxymethyl)-2,6-dimethylhept-6-enoic acid
show the reaction diagram
epsilon-caprolactone + H2O
?
show the reaction diagram
ethyl caproate + H2O
?
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,2'-dipyridyl
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1 mM, 55% inhibition
2-Bromo-4'-nitroacetophenone
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1 mM, 84% inhibition
CaCl2
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1 mM, 27% inhibition
CoCl2
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1 mM, 71% inhibition
EDTA
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10 mM, 16% inhibition
HgCl2
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0.1 mM 95% inhibition
MnCl2
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1 mM, 37% inhibition
p-chloromercuribenzoate
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0.1 mM, 26% inhibition
phenylhydrazine
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1 mM, 17% inhibition
SDS
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1 mM, 78% inhibition
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 10.5
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pH 7.5: about 60% of maximal activity, pH 10.5: about 50% of maximal activity
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
13 - 37
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13°C: about 35% of maximal activity, 37°C: 75% of maximal activity
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35000
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gel filtration
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
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inactivation above
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, MLH can be stored for 6 months, without loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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Show AA Sequence (472 entries)
Please use the Sequence Search for a specific query.
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LINKS TO OTHER DATABASES (specific for EC-Number 3.1.1.83)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)