This enzyme is a serine hydrolase. Compared with other lipases, hormone-sensitive lipase has a uniquely broad substrate specificity. It hydrolyses all acylglycerols (triacylglycerol, diacylglycerol and monoacylglycerol) [2,3,4] as well as cholesteryl esters [2,4], steroid fatty acid esters , retinyl esters and p-nitrophenyl esters [4,7]. It exhibits a preference for the 1- or 3-ester bond of its acylglycerol substrate compared with the 2-ester bond . The enzyme shows little preference for the fatty acids in the triacylglycerol, although there is some increase in activity with decreasing chain length. The enzyme activity is increased in response to hormones that elevate intracellular levels of cAMP.
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SYSTEMATIC NAME
IUBMB Comments
diacylglycerol acylhydrolase
This enzyme is a serine hydrolase. Compared with other lipases, hormone-sensitive lipase has a uniquely broad substrate specificity. It hydrolyses all acylglycerols (triacylglycerol, diacylglycerol and monoacylglycerol) [2,3,4] as well as cholesteryl esters [2,4], steroid fatty acid esters [5], retinyl esters [6] and p-nitrophenyl esters [4,7]. It exhibits a preference for the 1- or 3-ester bond of its acylglycerol substrate compared with the 2-ester bond [8]. The enzyme shows little preference for the fatty acids in the triacylglycerol, although there is some increase in activity with decreasing chain length. The enzyme activity is increased in response to hormones that elevate intracellular levels of cAMP.
in periparturient dairy cows, the compound inhibits basal lipolysis negligibly at 11 d prepartum, but at 11 and 24 d postpartum it reduces basal lipolysis by 36.1% and 43.1%, respectively
stearate and palmitate, saturated fatty acids but not unsaturated fatty acids (oleate and linoleate) induce up-regulation of HSL mRNA in a time- and concentration-dependent manner in bMEC. Oleate and linoleate induce no up-regulation of HSL mRNA
stearate and palmitate, saturated fatty acids but not unsaturated fatty acids (oleate and linoleate) induce up-regulation of HSL mRNA in a time- and concentration-dependent manner in bMEC. Oleate and linoleate induce no up-regulation of HSL mRNA
HSL contribution to basal lipolysis is negligible prepartum in periparturient dairy cows. HSL is a major driver of subcutaneous adipose tissue lipolytic responses postpartum. Reduced lipogenesis is an important contributor to fatty acid release from subcutaneous adipose tissue
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 500 mM phosphate buffer, pH 7.0, 50% glycerol, 100 mM benzamidine, 1 mM dithiothreitol, 0.2% C13E12, 5 mg/ml leupeptin and 1 mg/ml pepstatin, at least 2 months, no loss of activity