Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 3.1.1.73 - feruloyl esterase and Organism(s) Talaromyces funiculosus and UniProt Accession Q9HE18

for references in articles please use BRENDA:EC3.1.1.73
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.73 feruloyl esterase
IUBMB Comments
Catalyses the hydrolysis of the 4-hydroxy-3-methoxycinnamoyl (feruloyl) group from an esterified sugar, which is usually arabinose in "natural" substrates. p-Nitrophenol acetate and methyl ferulate are poorer substrates. All microbial ferulate esterases are secreted into the culture medium. They are sometimes called hemicellulase accessory enzymes, since they help xylanases and pectinases to break down plant cell wall hemicellulose.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Talaromyces funiculosus
UNIPROT: Q9HE18
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
hide
The taxonomic range for the selected organisms is: Talaromyces funiculosus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
feruloyl esterase, ferulic acid esterase, cinii, anfaea, feruloyl esterase a, fae-iii, cinnae, type a feruloyl esterase, estf27, xylanase z, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ferulic acid esterase
-
ferulic acid esterase B
-
feruloyl esterase
-
cinnAE
-
-
-
-
cinnamoyl ester hydrolase
-
-
-
-
Cinnamoyl esterase
-
-
-
-
EstA
-
-
-
-
FAE-I
-
-
-
-
FAE-II
-
-
-
-
FAE-III
-
-
-
-
ferulic acid esterase
ferulic acid esterase B
-
-
feruloyl/p-coumaroyl esterase
-
-
-
-
Feruloylesterase
-
-
-
-
hemicellulase acessory enzymes
-
-
-
-
hydroxycinnamoyl esterase
-
-
-
-
phenolic acid esterase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
carboxylic ester hydrolysis
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
4-hydroxy-3-methoxycinnamoyl-sugar hydrolase
Catalyses the hydrolysis of the 4-hydroxy-3-methoxycinnamoyl (feruloyl) group from an esterified sugar, which is usually arabinose in "natural" substrates. p-Nitrophenol acetate and methyl ferulate are poorer substrates. All microbial ferulate esterases are secreted into the culture medium. They are sometimes called hemicellulase accessory enzymes, since they help xylanases and pectinases to break down plant cell wall hemicellulose.
CAS REGISTRY NUMBER
COMMENTARY hide
134712-49-5
-
224306-54-1
-
224306-55-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
methyl caffeate + H2O
caffeate + methanol
show the reaction diagram
-
-
-
?
methyl coumarate + H2O
coumaric acid + methanol
show the reaction diagram
-
-
-
?
methyl ferulate + H2O
ferulic acid + methanol
show the reaction diagram
1-naphthyl acetate + H2O
acetic acid + 1-naphthol
show the reaction diagram
-
-
-
-
?
acetylxylan + H2O
acetic acid + ?
show the reaction diagram
-
-
-
-
?
de-starched wheat bran + H2O
ferulic acid + ?
show the reaction diagram
-
-
-
-
?
ferulic acid methyl ester + H2O
ferulic acid + methanol
show the reaction diagram
-
-
-
-
?
feruloyl arabinoside + H2O
ferulic acid + coumaric acid + cinnamic acid + arabinose
show the reaction diagram
-
-
-
-
?
feruloyl-polysaccharide + H2O
ferulate + polysaccharide
show the reaction diagram
-
-
-
-
?
methyl caffeate + H2O
caffeic acid + methanol
show the reaction diagram
-
-
-
-
?
methyl ferulate + H2O
ferulic acid + methanol
show the reaction diagram
-
-
-
?
O-5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O
ferulic acid + alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose
show the reaction diagram
-
FAXX
-
-
?
p-coumaric acid methyl ester + H2O
p-coumaric acid + methanol
show the reaction diagram
-
-
-
-
?
sugar beet pulp + H2O
ferulic acid + ?
show the reaction diagram
-
untreated sugar beet pulp
-
-
?
triacetin + H2O
acetate + glycerol
show the reaction diagram
-
-
-
-
?
wheat bran + H2O
ferulic acid + p-coumaric acid + ?
show the reaction diagram
-
untreated wheat bran
-
-
?
[5-O-(trans-feruloyl)-alpha-L-arabinofuranosyl]-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylopyranoside + H2O
ferulate + alpha-L-arabinofuranosyl]-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylopyranoside
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
feruloyl-polysaccharide + H2O
ferulate + polysaccharide
show the reaction diagram
-
-
-
-
?
sugar beet pulp + H2O
ferulic acid + ?
show the reaction diagram
-
untreated sugar beet pulp
-
-
?
wheat bran + H2O
ferulic acid + p-coumaric acid + ?
show the reaction diagram
-
untreated wheat bran
-
-
?
additional information
?
-
the expression of the FAE-encoding gene is regulated by xylose, arabinose and ferulic acid
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.139
methyl caffeate
37°C, pH 6.0
0.007
methyl coumarate
37°C, pH 6.0
0.047
methyl ferulate
37°C, pH 6.0
0.0246
alpha-L-arabinofuranosyl]-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylopyranoside
-
-
-
0.139
methyl caffeate
-
-
0.0472
methyl ferulate
-
-
0.0068
methyl p-coumarate
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
24.1
methyl caffeate
37°C, pH 6.0
24.2
methyl coumarate
37°C, pH 6.0
16
methyl ferulate
37°C, pH 6.0
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.25
-
acetylated xylan as substrate
0.49
-
alpha-naphthyl acetate as substrate
0.7
-
triacetin as substrate
22.5
-
methyl ferulate as substrate
24.6
-
FAXX as substrate
36.1
-
methyl p-coumarate as substrate
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70.4
differential scanning microcalorimetry (DSC) using a Microcal model VP-DSC calorimeter, pH 5.0 in SEC buffer
66.3
differential scanning microcalorimetry (DSC) using a Microcal model VP-DSC calorimeter, pH 5.0 in SEC buffer
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
FAEB_TALFU
353
1
37334
Swiss-Prot
Secretory Pathway (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35450
-
predicted molecular mass of mature polypeptide
40000
-
mass spectrometry
53000
-
SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
precipitation, anion exchange chromatography
precipitation, anion exchange chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Aspergillus awamori strain ATCC 22342
expression in Aspergillus awamori strain ATCC 22342
gene fragment containing 1120-bp ORF for faeB obtained by PCR, cloned and expressed in Escherichia coli XL1-blue MRF
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kroon, P.A.; Williamson, G.; Fish, N.M.; Archer, D.B.; Belshaw, N.J.
A modular esterase from Penicillium funiculosum which releases ferulic acid from plant cell walls and binds crystalline cellulose contains a carbohydrate binding module
Eur. J. Biochem.
267
6740-6752
2000
Aspergillus awamori, Aspergillus niger, Aspergillus sp., Butyrivibrio fibrisolvens, Acetivibrio thermocellus, Orpinomyces sp., Talaromyces funiculosus, Piromyces sp. 'equi', Talaromyces funiculosus IMI-134756
Manually annotated by BRENDA team
Fazary, A.E.; Ju, Y.H.
Feruloyl esterases as biotechnological tools: current and future perspectives
Acta Biochim. Biophys. Sin.
39
811-828
2007
Aspergillus nidulans, Aspergillus sp., Aureobasidium pullulans, Thermothelomyces heterothallicus, Thermoclostridium stercorarium, Acetivibrio thermocellus, Fusarium oxysporum, Penicillium expansum, Talaromyces pinophilus, Talaromyces stipitatus, Pseudomonas fluorescens, Streptomyces olivochromogenes, Fusarium proliferatum, Aspergillus niger (O42807), Talaromyces funiculosus (Q9HE18), Neurospora crassa (Q9HGR3), Aspergillus awamori (Q9P979)
Manually annotated by BRENDA team
Wong, D.W.
Feruloyl esterase: a key enzyme in biomass degradation
Appl. Biochem. Biotechnol.
133
87-112
2006
Aspergillus awamori, Aspergillus oryzae, Acetivibrio thermocellus, Fusarium oxysporum, Neocallimastix sp., Penicillium expansum, Pseudomonas fluorescens, Streptomyces olivochromogenes, Aspergillus niger (O42807), Talaromyces funiculosus (Q9HE18), Neurospora crassa (Q9HGR3), Piromyces sp. 'equi' (Q9Y871)
Manually annotated by BRENDA team
Topakas, E.; Vafiadi, C.; Christakopoulos, P.
Microbial production, characterization and applications of feruloyl esterases
Proc. Biochem.
42
497-509
2007
Aspergillus flavipes, Aspergillus foetidus, Aspergillus phoenicis, Aspergillus terreus, Bacillus subtilis, Fibrobacter succinogenes, Fusarium oxysporum, Fusarium verticillioides, Lactobacillus acidophilus, Limosilactobacillus fermentum, Neocallimastix sp., Neurospora crassa, Neurospora crassa (Q9HGR3), Penicillium brevicompactum, Penicillium expansum, Talaromyces pinophilus, Talaromyces stipitatus, Talaromyces stipitatus (Q70Y21), Streptomyces olivochromogenes, Streptococcus thermophilus, Streptomyces avermitilis, Trichoderma reesei, Fusarium proliferatum, Penicillium brasilianum, Piromyces MC-1, Cellvibrio japonicus (Q51815), Talaromyces funiculosus (Q9HE18), Orpinomyces sp. (Q9P8Y0), Piromyces sp. 'equi' (Q9Y871), Aspergillus terreus VTT-D-82209, Fibrobacter succinogenes S851, Streptomyces avermitilis CECT 3339, Aspergillus phoenicis IMI 211395, Aspergillus foetidus VTT-D-71002
-
Manually annotated by BRENDA team
Knoshaug, E.P.; Selig, M.J.; Baker, J.O.; Decker, S.R.; Himmel, M.E.; Adney, W.S.
Heterologous expression of two ferulic acid esterases from Penicillium funiculosum
Appl. Biochem. Biotechnol.
146
79-87
2008
Talaromyces funiculosus (Q8WZI7), Talaromyces funiculosus (Q9HE18)
Manually annotated by BRENDA team
Koseki, T.; Fushinobu, S.; Ardiansyah, S.; Shirakawa, H.; Komai, M.
Occurrence, properties, and applications of feruloyl esterases
Appl. Microbiol. Biotechnol.
84
803-810
2009
Aspergillus luchuensis, Aspergillus niger, Aspergillus niger (O42807), Aspergillus niger (Q8WZI8), Aspergillus oryzae, Aureobasidium pullulans, Thermothelomyces heterothallicus, Acetivibrio thermocellus, Fusarium oxysporum, Neocallimastix sp., Talaromyces stipitatus, Cellvibrio japonicus, Salmonella enterica subsp. enterica serovar Typhimurium (G2QND5), Aspergillus tubingensis (O42815), Penicillium chrysogenum (Q3V6C9), Aspergillus nidulans (Q5BCF8), Piromyces sp. E2 (Q870B0), Talaromyces funiculosus (Q9HE18), Neurospora crassa (Q9HGR3), Orpinomyces sp. PC-2 (Q9P8Y0), Aspergillus awamori (Q9P979), Piromyces sp. 'equi' (Q9Y871)
Manually annotated by BRENDA team