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Information on EC 3.1.1.72 - acetylxylan esterase and Organism(s) Aspergillus oryzae and UniProt Accession Q75P26

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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.72 acetylxylan esterase
IUBMB Comments
Catalyses the hydrolysis of acetyl groups from polymeric xylan, acetylated xylose, acetylated glucose, alpha-napthyl acetate, p-nitrophenyl acetate but not from triacetylglycerol. Does not act on acetylated mannan or pectin.
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This record set is specific for:
Aspergillus oryzae
UNIPROT: Q75P26
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The taxonomic range for the selected organisms is: Aspergillus oryzae
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Reaction Schemes
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acetylxylan
+
3
H2O
=
3
acetic acid
+
xylan
+
3
=
3
+
Synonyms
xylanase, chloroacetate esterase, acetyl xylan esterase, acetyl esterase, xyn10b, acetylxylan esterase, axe ii, axe i, xyns20e, acetyl xylan esterase 1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acetic ester hydrolase
-
-
-
-
Acetyl esterase
-
-
-
-
Acetylglucomannan esterase
-
-
-
-
Acetylnaphthylesterase
-
-
-
-
Acetyte esterase
-
-
-
-
C-esterase
-
-
-
-
Chloroacetate esterase
-
-
-
-
Chloroesterase
-
-
-
-
Citrus acetylesterase
-
-
-
-
Esterase, acetyl
-
-
-
-
Esterase, C-
-
-
-
-
Heroin esterase
-
-
-
-
N-Acetylphosphinothricin deacetylase
-
-
-
-
Naphthal AS-D chloroacetate deacetylase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of carboxylic ester
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
acetylxylan esterase
Catalyses the hydrolysis of acetyl groups from polymeric xylan, acetylated xylose, acetylated glucose, alpha-napthyl acetate, p-nitrophenyl acetate but not from triacetylglycerol. Does not act on acetylated mannan or pectin.
CAS REGISTRY NUMBER
COMMENTARY hide
188959-24-2
-
9000-82-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-naphthyl acetate + H2O
1-naphthol + acetate
show the reaction diagram
-
-
-
?
acetylated birchwood xylan + H2O
acetate + birchwood xylan
show the reaction diagram
-
-
-
?
alpha-naphthyl propionate + H2O
1-naphthol + propionate
show the reaction diagram
-
-
-
?
additional information
?
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 8
pH 5.0: about 40% of maximal activity, pH 8.0: about 50% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35 - 55
35°C: about 75% of maximal activity, 55°C: about 60% of maximal activity
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
Uniprot
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
recombinant AoAXE is secreted by the aid of fused alpha-factor secretion signal peptide
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
overexpression in Trichoderma reesei by fusing with xylanase Xyn2 from Trichoderma reesei as a carrier protein. The production of AXE in the transformant T2 is approximately 74% higher than that of the parent strain. The saccharification efficiency of rAXE gives approximately 23.2%, 22.7%, and 19.4% higher yield of reducing sugar in paddy straw, corn stover plus paddy straw mix (1:1 ratio), and corn stalk (delignified) hydrolysis
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
31000
x * 31000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 31000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
the recombinant enzyme appears to possess ASn-linked high-mannose oligosaccharides
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
room temperature, 1 h, about 50% loss of activity
665786
6 - 7
room temperature, 1 h, stable
665786
8
room temperature, 1 h, about 10% loss of activity
665786
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Pichia pastoris, recombinant AoAXE is secreted by the aid of fused alpha-factor secretion signal peptide
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Koseki, T.; Miwa, Y.; Akao, T.; Akita, O.; Hashizume, K.
An Aspergillus oryzae acetyl xylan esterase: molecular cloning and characteristics of recombinant enzyme expressed in Pichia pastoris
J. Biotechnol.
121
381-389
2006
Aspergillus oryzae (Q75P26), Aspergillus oryzae
Manually annotated by BRENDA team
Manavalan, T.; Liu, R.; Zhou, Z.; Zou, G.
Optimization of acetyl xylan esterase gene expression in Trichoderma reesei and its application to improve the saccharification efficiency on different biomasses
Process Biochem.
58
160-166
2017
Aspergillus oryzae, Aspergillus oryzae NBRC 100959
-
Manually annotated by BRENDA team