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Information on EC 3.1.1.72 - acetylxylan esterase and Organism(s) Aspergillus niger and UniProt Accession A2QZI3

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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.72 acetylxylan esterase
IUBMB Comments
Catalyses the hydrolysis of acetyl groups from polymeric xylan, acetylated xylose, acetylated glucose, alpha-napthyl acetate, p-nitrophenyl acetate but not from triacetylglycerol. Does not act on acetylated mannan or pectin.
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This record set is specific for:
Aspergillus niger
UNIPROT: A2QZI3
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The taxonomic range for the selected organisms is: Aspergillus niger
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Reaction Schemes
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acetylxylan
+
3
H2O
=
3
acetic acid
+
xylan
+
3
=
3
+
Synonyms
xylanase, chloroacetate esterase, acetyl xylan esterase, acetyl esterase, xyn10b, acetylxylan esterase, axe ii, axe i, acetyl xylan esterase 1, c-esterase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acetic ester hydrolase
-
-
-
-
Acetyl esterase
acetyl xylan esterase
-
-
acetyl xylan esterase 1
-
-
Acetylglucomannan esterase
-
-
-
-
Acetylnaphthylesterase
-
-
-
-
Acetyte esterase
-
-
-
-
C-esterase
-
-
-
-
Chloroacetate esterase
-
-
-
-
Chloroesterase
-
-
-
-
Citrus acetylesterase
-
-
-
-
Esterase, acetyl
-
-
-
-
Esterase, C-
-
-
-
-
Heroin esterase
-
-
-
-
N-Acetylphosphinothricin deacetylase
-
-
-
-
Naphthal AS-D chloroacetate deacetylase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of carboxylic ester
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
acetylxylan esterase
Catalyses the hydrolysis of acetyl groups from polymeric xylan, acetylated xylose, acetylated glucose, alpha-napthyl acetate, p-nitrophenyl acetate but not from triacetylglycerol. Does not act on acetylated mannan or pectin.
CAS REGISTRY NUMBER
COMMENTARY hide
188959-24-2
-
9000-82-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2 AcUXOS-2,3-di-O-acetyl-beta-D-xylopyranose + 2 H2O
AcUXOS-2-O-acetyl-beta-D-xylopyranose + AcUXOS-3-O-acetyl-beta-D-xylopyranose + 2 acetate
show the reaction diagram
-
AcUXOS: acetylated 4-O-methyl-glucuronic acid (MeGlcA)-substituted xylo-oligosaccharides; preferred subunit for deacetylation by isoform CE5
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + D-xylose
show the reaction diagram
-
-
-
-
?
acetylxylan
acetate + ?
show the reaction diagram
-
steamed birchwood xylan
-
-
?
AcUXOS-2,3-di-O-acetyl-beta-D-xylopyranose + 2 H2O
AcUXOS-beta-D-xylopyranose + 2 acetate
show the reaction diagram
-
AcUXOS: acetylated 4-O-methyl-glucuronic acid (MeGlcA)-substituted xylo-oligosaccharides; 2,3-di-O acetylated Xylp were nearly completely deacetylated by CE5
-
-
?
AcUXOS-2-O-acetyl-beta-D-xylopyranose + H2O
AcUXOS-beta-D-xylopyranose + acetate
show the reaction diagram
-
AcUXOS: acetylated 4-O-methyl-glucuronic acid (MeGlcA)-substituted xylo-oligosaccharides
-
-
?
AcUXOS-3-O-acetyl-2-O-(4-methyl-alpha-D-glucopyranuronosyl)-beta-D-xylopyranose + H2O
AcUXOS-2-O-(4-methyl-alpha-D-glucopyranuronosyl)-beta-D-xylopyranose + acetate
show the reaction diagram
-
AcUXOS: acetylated 4-O-methyl-glucuronic acid (MeGlcA)-substituted xylo-oligosaccharides
-
-
?
AcUXOS-3-O-acetyl-beta-D-xylopyranose + H2O
AcUXOS-beta-D-xylopyranose + acetate
show the reaction diagram
-
AcUXOS: acetylated 4-O-methyl-glucuronic acid (MeGlcA)-substituted xylo-oligosaccharides; preferred subunit for deacetylation by isoform CE16
-
-
?
an acetic ester + H2O
an alcohol + acetate
show the reaction diagram
-
-
-
-
?
hardwood acetyl glucuronoxylan
?
show the reaction diagram
-
the enzyme from Aspergillus niger is highly active compared to other acetylxylan esterases independently of the degree of polymerisation of the substrate. Mode of action, substrate specificity, and role of the family CE1 enzyme during deacetylation of soluble natural substrates, i.e. neutral and acidic O-acetylated xylooligosaccharides
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
an acetic ester + H2O
an alcohol + acetate
show the reaction diagram
-
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NiCl2
-
1 mM, 8% inhibition
Pb(NO3)2
-
1 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
the enzyme is not activated by acetyl esterases
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 6
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
-
assay at
40 - 50
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
industrial strain, Aspergillus niger/Talaromyces emersonii enzyme mixture containing Aspergillus niger acetylxylan esterase and Tlaromyces emersonii acetyl esterase
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
recombinant protein in expressed in Arabidopsis thaliana
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
expression in Arabidopsis thaliana. The protein is targeted to the apoplast by its native signal peptide, resulting in elevated acetyl esterase activity in soluble and wall-bound protein extracts and reduced xylan acetylation. No significant alterations in cell wall composition are observed. The xylans of transgenic lines are more easily digested by a beta-1,4-endoxylanase, and more readily extracted by hot water, acids or alkali. Transgenic plants develop normally and show increased resistance to the biotrophic pathogen Hyaloperonospora arabidopsidis
evolution
physiological function
-
acetylxylan esterases enhance the hydrolysis of O-acetylated xylan by removing the acetyl substituents that restrict the action of endo-1,4-beta-xylanases and beta-xylosidases
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30480
-
x * 30480, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 8
-
24 h, stable
23596
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
-
8 h, stable
50
-
6 h, 50% loss of activity
55
-
1 h, 50% loss of activity
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme from Aspergillus niger/Talaromyces emersonii enzyme mixture containing Aspergillus niger acetylxylan esterase and Talaromyces emersonii acetyl esterase by anion exchange chromatography, ultrafiltration, and gel filtration
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kormelink, F.J.M.; Lefebvre, B.; Strozyk, F.; Voragen, A.G.J.
Purification and characterization of an acetyl xylan esterase from Aspergillus niger
J. Biotechnol.
27
267-282
1993
Aspergillus niger
-
Manually annotated by BRENDA team
Christov, L.P.; Prior, B.A.
Esterases of xylan-degrading microorganisms: production, properties, and significance
Enzyme Microb. Technol.
15
460-475
1993
Aspergillus awamori, Aspergillus japonicus, Aspergillus nidulans, Aspergillus niger, Bacillus subtilis, Streptomyces sp., Fusarium oxysporum, no activity in Butyrivibrio fibrisolvens, no activity in Fibrobacter succinogenes, no activity in Neocallimastix sp., no activity in Orpinomyces sp., Rhodotorula mucilaginosa, Schizophyllum commune, Streptomyces olivochromogenes, Streptomyces flavogriseus, Thermobifida fusca, Trichoderma reesei, Streptomyces flavogriseus 45-CD, Fusarium oxysporum VTT-D-80134, Rhodotorula mucilaginosa NRC 211003, no activity in Butyrivibrio fibrisolvens NCFB 2249, no activity in Neocallimastix sp. MC-2, no activity in Fibrobacter succinogenes S85, Thermobifida fusca BS 21, Aspergillus awamori VTT-D-75028, no activity in Orpinomyces sp. PC-3
Manually annotated by BRENDA team
Neumueller, K.G.; Streekstra, H.; Gruppen, H.; Schols, H.A.
Trichoderma longibrachiatum acetyl xylan esterase 1 enhances hemicellulolytic preparations to degrade corn silage polysaccharides
Biores. Technol.
163
64-73
2014
Aspergillus niger, Trichoderma longibrachiatum
Manually annotated by BRENDA team
Koutaniemi, S.; van Gool, M.P.; Juvonen, M.; Jokela, J.; Hinz, S.W.; Schols, H.A.; Tenkanen, M.
Distinct roles of carbohydrate esterase family CE16 acetyl esterases and polymer-acting acetyl xylan esterases in xylan deacetylation
J. Biotechnol.
168
684-692
2013
Aspergillus niger, Trichoderma reesei, Thermothelomyces thermophilus
Manually annotated by BRENDA team
Neumueller, K.; De Souza, A.; Van Rijn, J.; Streekstra, H.; Gruppen, H.; Schols, H.
Positional preferences of acetyl esterases from different CE families towards acetylated 4-O-methyl glucuronic acid-substituted xylo-oligosaccharides
Biotechnol. Biofuels
8
7-7
2015
Aspergillus niger, Acetivibrio thermocellus, Rasamsonia emersonii (E9M3D1), Orpinomyces sp. PC-2 (O59869), Trichoderma reesei (Q99034)
Manually annotated by BRENDA team
Pawar, P.M.; Derba-Maceluch, M.; Chong, S.L.; Gomez, L.D.; Miedes, E.; Banasiak, A.; Ratke, C.; Gaertner, C.; Mouille, G.; McQueen-Mason, S.J.; Molina, A.; Sellstedt, A.; Tenkanen, M.; Mellerowicz, E.J.
Expression of fungal acetyl xylan esterase in Arabidopsis thaliana improves saccharification of stem lignocellulose
Plant Biotechnol. J.
14
387-397
2016
Aspergillus niger (A2QZI3), Aspergillus niger, Aspergillus niger FGSC A1513 (A2QZI3)
Manually annotated by BRENDA team