Information on EC 3.1.1.70 - cetraxate benzylesterase

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The expected taxonomic range for this enzyme is: Aspergillus niger

EC NUMBER
COMMENTARY hide
3.1.1.70
-
RECOMMENDED NAME
GeneOntology No.
cetraxate benzylesterase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
cetraxate benzyl ester + H2O = cetraxate + benzyl alcohol
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of carboxylic ester
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SYSTEMATIC NAME
IUBMB Comments
cetraxate-benzyl-ester benzylhydrolase
Acts on a number of benzyl esters of substituted phenyl propanoates, and on the benzyl esters of phenylalanine and tyrosine.
CAS REGISTRY NUMBER
COMMENTARY hide
125858-78-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
benzyl esters of substituted phenyl propanoates + H2O
benzyl alcohol + substituted phenyl propanoate
show the reaction diagram
-
benzyl esters of aliphatic and aromatic carboxylic acids, benzyl esters of phenylalanine Phe-By and tyrosine Tyr-By are hydrolyzed
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-
?
cetraxate benzyl ester + H2O
cetraxate + benzyl alcohol
show the reaction diagram
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-
?
L-Phe benzyl ester + H2O
benzyl alcohol + L-phenylalanine
show the reaction diagram
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-
-
-
?
L-Tyr benzyl ester + H2O
benzyl alcohol + L-tyrosine
show the reaction diagram
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-
-
-
?
additional information
?
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enzyme shows no cellulase, protease or lipase activity, does not hydrolyze ester groups such as Tyr-Me, Tyr-tert-Bu, 1-Me, 1-iso-Pro and 1 Ph
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
cetraxate benzyl ester + H2O
cetraxate + benzyl alcohol
show the reaction diagram
-
-
-
?
L-Phe benzyl ester + H2O
benzyl alcohol + L-phenylalanine
show the reaction diagram
-
-
-
-
?
L-Tyr benzyl ester + H2O
benzyl alcohol + L-tyrosine
show the reaction diagram
-
-
-
-
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ca2+
-
1.0 mM, 71% relative residual activity
Co2+
-
1.0 mM, 68% relative residual activity
Cu2+
-
1.0 mM, 72% relative residual activity
diisopropyl phosphofluoridate
-
9% relative residual activity
Fe2+
-
1.0 mM, 81% relative residual activity
Hg2+
-
9% relative residual activity
Mg2+
-
1.0 mM, 64% relative residual activity
Mn2+
-
1.0 mM, 79% relative residual activity
Ni2+
-
1.0 mM, 67% relative residual activity
o-phenanthroline
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80% relative residual activity
Zn2+
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1.0 mM, 70% relative residual activity
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4
benzyl cetraxate
-
pH 4.5, 30°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
specific activity 791 units/mg
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5
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for debenzylating substrate
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
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debenzylation
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.3
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isoelectric focusing, carrier ampholite, pH range from 3.5-10.0 at 200 V for 30 min and 700 V for 90 min
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
Sumizyme AC, Shin-Nippon Chemical Industries Co., Ltd., cellulase activity 2-5 unit/mg
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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1 * 35000, SDS-PAGE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 7
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stable in this range
643849
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
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stable at temperatures below
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
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cetraxate hydrochloride is a widely used antiulcer agent industrially produced