Information on EC 3.1.1.68 - xylono-1,4-lactonase

for references in articles please use BRENDA:EC3.1.1.68
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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.1.1.68
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RECOMMENDED NAME
GeneOntology No.
xylono-1,4-lactonase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-xylono-1,4-lactone + H2O = D-xylonate
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of carboxylic ester
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
d-xylose degradation
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SYSTEMATIC NAME
IUBMB Comments
D-xylono-1,4-lactone lactonohydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
113066-39-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene xylC
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Manually annotated by BRENDA team
subsp. suboxydans ATCC 621
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Manually annotated by BRENDA team
ATCC 4973
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-xylono-1,4-lactone + H2O
D-xylonate
show the reaction diagram
additional information
?
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unable to utilize ribono-gamma-lactone as substrate
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-xylono-1,4-lactone + H2O
D-xylonate
show the reaction diagram
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
D-xylono-1,4-lactone
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enzyme induced by
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene xylC, functional co-expression of a xylose dehydrogenase (gene xdh) and a xylonolactonase (gene xylC) from Caulobacter crescentus in Escherichia coli strain BL21DELTAxylAB, lacking functional genes xylA and xylB encoding xylose isomerase and xylulose kinase, which compete for D-xyylose with xylose dehydrogenase
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gene xylC, functional expression in Saccharomyces cerevisiae, co-expression with D-xylose dehydrogenase, gene xylB, product determinations by NMR spectroscopy and pHluorin fluorescence measurement
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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an engineered Escherichia coli strain, with functional co-expression of a xylose dehydrogenase (gene xdh) and a xylonolactonase (gene xylC) from Caulobacter crescentus, has a promising perspective for large-scale production of xylonate
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotechnology
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considerable interest exists in utilizing the hemicellulose biomass for fine chemical production by converting xylose microbially to xylonic acid, other proposed uses of xylonic acid are in textile bleaching or in electroplating
synthesis
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an engineered Escherichia coli strain, with functional co-expression of a xylose dehydrogenase (gene xdh) and a xylonolactonase (gene xylC) from Caulobacter crescentus, has a promising perspective for large-scale production of xylonate