Information on EC 3.1.1.63 - 11-cis-retinyl-palmitate hydrolase

for references in articles please use BRENDA:EC3.1.1.63
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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.1.1.63
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RECOMMENDED NAME
GeneOntology No.
11-cis-retinyl-palmitate hydrolase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
11-cis-retinyl palmitate + H2O = 11-cis-retinol + palmitate
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of carboxylic ester
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Retinol metabolism
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SYSTEMATIC NAME
IUBMB Comments
11-cis-retinyl-palmitate acylhydrolase
Activated by bile salts.
CAS REGISTRY NUMBER
COMMENTARY hide
106389-23-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
11-cis-retinyl palmitate + H2O
11-cis-retinol + palmitate
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
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the hydrolysis of 11-cis retinyl esters in the retinal pigment epithelium membrane constitutes a novel pathway of visual chromophore biosynthesis
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
all-trans-retinyl bromoacetate
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0.05 mM 50% inhibition
all-trans-retinyl palmitate
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weak inhibition
NEM
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inhibits all-trans retinyl-palmitate hydrolase and 11-cis-retinyl-palmitate hydrolase activity of the liver enzyme, inhibits all-trans-retinyl-palmitate hydrolase activity of the pigment epithelium, 1-cis-retinyl-palmitate hydrolase activity of the pigment epithelium is unaffected
triolein
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inhibits all-trans retinyl-palmitate hydrolase and 11-cis-retinyl-palmitate hydrolase activity of the liver enzyme, inhibits all-trans-retinyl-palmitate hydrolase activity of the pigment epithelium, 1-cis-retinyl-palmitate hydrolase activity of the pigment epithelium is unaffected
additional information
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the distinctive apparent inhibition constants for NEM-induced inhibition of 11-cis retinyl-palmitate hydrolase and the all-trans-retinyl-palmitate hydrolase activity suggest a fundamental difference in the amino acid composition at the active site of the enzyme(s)
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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no detergents required
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.004 - 0.66
11-cis-retinyl palmitate
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.05
all-trans-retinyl bromoacetate
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8 - 9
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8.5
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addition of 1.0% sodium taurocholate
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 9.5
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pH 7.0: about 60% of maximal activity, pH 9.5: about 30% of maximal activity
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
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Manually annotated by BRENDA team
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
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10 min, complete loss of activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, overnight storage of cell homogenate, no loss of activity
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