Information on EC 3.1.1.60 - bis(2-ethylhexyl)phthalate esterase

for references in articles please use BRENDA:EC3.1.1.60
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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.1.1.60
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RECOMMENDED NAME
GeneOntology No.
bis(2-ethylhexyl)phthalate esterase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
bis(2-ethylhexyl)phthalate + H2O = 2-ethylhexyl phthalate + 2-ethylhexan-1-ol
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of carboxylic ester
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SYSTEMATIC NAME
IUBMB Comments
bis(2-ethylhexyl)phthalate acylhydrolase
Also acts on 4-nitrophenyl esters, with optimum chain-length C6 to C8.
CAS REGISTRY NUMBER
COMMENTARY hide
92480-02-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
show the reaction diagram
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4-nitrophenyl acylester + H2O
4-nitrophenol + carboxylic acid
show the reaction diagram
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with optimum chain-length C6 to C8, cleavage of bis(2-ethylhexyl)phthalate is 10000times slower than cleavage of 4-nitrophenyl octanoate
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?
bis(2-ethylhexyl)phthalate + H2O
2-ethylhexyl phthalate + 2-ethylhexan-1-ol
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
bis(2-ethylhexyl)phthalate + H2O
2-ethylhexyl phthalate + 2-ethylhexan-1-ol
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4-mercuribenzoate
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complete inhibition at 1 mM, 80% inhibition at 0.1 mM
4-nitrophenyl phosphate
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4-nitrophenyl-diethyl phosphate
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synonym: Paraoxon, complete inhibition at 0.1 and 1 mM
deoxycholate
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diisopropylfluorophosphate
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complete inhibition at 1 mM, 80% inhibition at 0.1 mM
Dimethylsulfoxide
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70% inihibition at 5%
dodecyl beta-maltoside
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eserine
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complete inhibition at 1 mM, 80% inhibition at 0.1 mM
ethyleneglycolmonomethyl ether
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70% inihibition at 5%
HgCl2
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complete inhibition at 1 mM, 80% inhibition at 0.1 mM
Lubrol Px
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phenylmethylsulfonyl fluoride
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75% inhibition at 1 mM
taurocholate
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Triton X-100
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Tween-20
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Tween-80
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additional information
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cleavage of bis(2-ethylhexyl)phthalate is strongly inhibited by a wheat cell wall and by a triglyceride preparation, cleavage of the water-soluble substrate, 4-nitrophenylacetate is not inhibited
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.005
bis(2-ethylhexyl)phthalate
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30°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0000833
bis(2-ethylhexyl)phthalate
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 9
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broad
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22000
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x * 22000, SDS-PAGE
38000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 22000, SDS-PAGE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, half-life: 1 week for the enzyme from first steps of purification, 1 day for the enzyme from last steps of purification and purified enzyme
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