Information on EC 3.1.1.59 - juvenile-hormone esterase

for references in articles please use BRENDA:EC3.1.1.59
Word Map on EC 3.1.1.59
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.1.1.59
-
RECOMMENDED NAME
GeneOntology No.
juvenile-hormone esterase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
juvenile hormone I + H2O = juvenile hormone I acid + methanol
show the reaction diagram
(1)
-
-
-
juvenile hormone III + H2O = juvenile hormone III acid + methanol
show the reaction diagram
(2)
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of carboxylic ester
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Insect hormone biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
methyl-(2E,6E)-(10R,11S)-10,11-epoxy-3,7,11-trimethyltrideca-2,6-dienoate acylhydrolase
Demethylates the insect juvenile hormones JH1 and JH3, but does not hydrolyse the analogous ethyl or isopropyl esters.
CAS REGISTRY NUMBER
COMMENTARY hide
50812-15-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
hybrid-strain Shogetu-Shunrei
-
-
Manually annotated by BRENDA team
spruce budworm
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Isia isabella
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
gene RfEst1
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
genes Tcjhe-a, Tcjhe-b, Tcjhe-c, Tcjhe-d, and Tcjhe-e; genes Tcjhe-a, Tcjhe-b, Tcjhe-c, Tcjhe-d, and Tcjhe-e, encoding isozymes that vary only in the 5'-UTR sequences
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-naphthyl acetate + H2O
1-naphthol + acetate
show the reaction diagram
1-Naphthyl methylcarbonate + H2O
1-Naphthol + methylcarbonate
show the reaction diagram
-
6% of the activity with juvenile hormone I
-
-
-
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
show the reaction diagram
-
69% of the activity with juvenile hormone I, slightly more active than with juvenile hormone III
-
-
-
4-Nitrophenyl methylcarbonate + H2O
4-Nitrophenol + methylcarbonate
show the reaction diagram
-
5% of the activity with juvenile hormone I
-
-
-
alpha-naphthyl acetate + H2O
?
show the reaction diagram
-
artificial substrate
-
?
alpha-naphthyl acetate + H2O
alpha-naphthol + acetate
show the reaction diagram
alpha-naphthyl butyrate + H2O
alpha-naphthol + butyrate
show the reaction diagram
alpha-naphthyl hexanoate + H2O
alpha-naphthol + hexanoate
show the reaction diagram
alpha-naphthyl octanoate + H2O
alpha-naphthol + octanoate
show the reaction diagram
alpha-naphthyl propionate + H2O
alpha-naphthol + propionate
show the reaction diagram
HEPTAT + H2O
?
show the reaction diagram
synthetic juvenile hormone analog
-
?
juvenile hormone + H2O
?
show the reaction diagram
Juvenile hormone + H2O
Juvenile hormone acid + acid diol
show the reaction diagram
juvenile hormone + H2O
juvenile hormone acid + methanol
show the reaction diagram
juvenile hormone I + H2O
juvenile hormone I acid + methanol
show the reaction diagram
juvenile hormone II + H2O
juvenile hormone II acid + methanol
show the reaction diagram
-
-
-
-
?
juvenile hormone III + H2O
?
show the reaction diagram
juvenile hormone III + H2O
juvenile hormone acid + methanol
show the reaction diagram
juvenile hormone III + H2O
juvenile hormone III acid + methanol
show the reaction diagram
juvenile hormone III bisepoxide + H2O
?
show the reaction diagram
juvenile hormone III diol + H2O
?
show the reaction diagram
-
although hydrogen bonding appears not to occur between juvenile hormone III diol and Thr-314, the hydroxyls of the diol (at C10 and C11) are within hydrogen bonding distance of the main chain carbonyl oxygen atoms of Phe-259 and Phe-311
-
-
?
methyl (2E,6E)-(10R,11S)-10,11-epoxy-3,7,11-trimethyltrideca-2,6-dienoate + H2O
(2E,6E)-(10R,11S)-10,11-epoxy-3,7,11-trimethyltrideca-2,6-dienoate + methanol
show the reaction diagram
juvenile hormone esterase plays an important role in regulating juvenile hormone titers
-
-
?
methyl farnesoate + H2O
methanol + farnesoic acid
show the reaction diagram
-
-
-
?
methyl heptylthioacetothioate + H2O
?
show the reaction diagram
methyl hexylthio acetothioate + H2O
?
show the reaction diagram
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
show the reaction diagram
p-nitrophenyl valerate + H2O
p-nitrophenol + valerate
show the reaction diagram
saturated juvenile hormone III + H2O
saturated juvenile hormone III acid + methanol
show the reaction diagram
-
alpha,beta-saturated methyl ester
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
juvenile hormone + H2O
?
show the reaction diagram
juvenile hormone + H2O
juvenile hormone acid + methanol
show the reaction diagram
juvenile hormone III + H2O
?
show the reaction diagram
juvenile hormone III + H2O
juvenile hormone acid + methanol
show the reaction diagram
-
JH esterase hydrolyzes the methyl ester to produce juvenile hormone acid
-
-
?
juvenile hormone III bisepoxide + H2O
?
show the reaction diagram
-
JHE is highly selective for JHIII and JHIII bisepoxide, both may be in vivo substrates
-
?
methyl (2E,6E)-(10R,11S)-10,11-epoxy-3,7,11-trimethyltrideca-2,6-dienoate + H2O
(2E,6E)-(10R,11S)-10,11-epoxy-3,7,11-trimethyltrideca-2,6-dienoate + methanol
show the reaction diagram
Q17AV1
juvenile hormone esterase plays an important role in regulating juvenile hormone titers
-
-
?
additional information
?
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,1,1-trifluoro-3-(octane-1-sulfonyl)propan-2,2-diol
IC50: 0.0016 mM
1,1,1-trifluoro-3-(octylsulfanyl)propan-2-one
-
-
1,1,1-trifluoro-3-decylsulfanyl-propan-2-one
1,1,1-trifluoro-3-dodecylsulfanyl-propan-2-one
1,1,1-trifluoro-3-hexylsulfanyl-propan-2-one
1,1,1-trifluoro-3-octylsulfanyl-propan-2-one
1,1,1-trifluoro-3-phenethylsulfanyl-propan-2-one
1-octyl (1-(3,3,3-trifluoropropan-2,2-dihydroxy))sulfone
-
potent, specific inhibitor
3-(4'-Butylphenylthio)-1,1,1-trifluoropropan-2-one
-
-
3-octylthio-1,1,1-trifluoro-2-propanone
3-Octylthio-1,1,1-trifluoropropan-2-one
3-pentylthio-1,1,1-trifluoro-2-propanone
3-Substituted 1,1,1-trifluoropropanone sulfides
3-[(4'-Mercapto)butylthio]-1,1,1-trifluoropropan-2-one
-
-
Alkylthiotrifluoropropanones
bis-(4-nitrophenyl) phosphate
-
-
Carbaryl
weak, IC50: above 0.1 mM
chlorpromazine
-
octopamine antagonist, reduces JHE activity, when applied in diet at 10-100 ppm
dichlorvos
-
-
diisopropyl fluorophosphate
eserine
-
IC50: 3.9-4.2, high- and low-selected JHE activity lines
gramine
-
octopamine antagonist, reduces JHE activity, when applied in diet at 10-100 ppm
hydroprene
50% inhibition at 8 mM
JHE binding protein
-
DmP29, induces anti-juvenile hormone effects
-
Juvenile hormone
-
juvenile hormone I, II or III
kinoprene
50% inhibition at 2 mM
methamidophos
weak, IC50: above 1 mM
methoprene
50% inhibition at 2 mM
O,O-Dialkyl-O-4-nitrophenyl phosphates
-
O,O-dialkyl-O-4-nitrophenyl analog provides the greatest inhibition
O,O-diethyl-p-nitrophenyl phosphate
O,O-diisopropyl phosphofluoridate
-
pI50: 5-5.7, high- and low-selected JHE activity lines
O-ethyl-S-phenyl-phosphoramidothiolate
IC50: 0.0084 mM
O-Ethyl-S-phosphoramidothiolate
octylthiotrifluoropropanone
50% inhibition below 1 nM
Paraoxon
Phenylmethylsulfonylfluoride
phosphoramidothiolate
-
JHE is highly sensitive to certain phosphoramidothiolates
trifluoroketone inhibitor
-
JHE is highly sensitive
-
Trifluoroketones
-
-
trifluoromethylketone
-
with chain lengths of 8, 10 or 12 carbons, highly effective inhibitors
Tris-butylphosphorotrithiolate
-
-
Triton X-100
ZR-448
-
a methyl ester dienoate juvenoid, competitive
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-(4-chloro-2-methylphenyl)imidazolidine-2-thione
-
octopamine agonist, stimulates the pupation and JHE activity of larvae reared under crowded conditions, when topically applied, 0.05 mg
2-(4-chloro-2-methylphenylimino)oxazolidine
-
octopamine agonist, stimulates the pupation and JHE activity of larvae reared under crowded conditions, when topically applied, 0.05 mg
acetone
chlordimeform
-
octopamine agonist, stimulates the pupation and JHE activity of larvae reared under crowded conditions, when topically applied, 0.01 mg
detergent
-
activates at lower concentrations
-
ethanol
-
activates even at higher concentrations
Isopropanol
-
activates even at higher concentrations
octopamine
organic solvent
-
activates at lower concentrations
-
Triton X-100
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.12 - 0.41
Alpha-naphthyl acetate
0.0191
HEPTAT
recombinant JHE
0.00015 - 0.00179
Juvenile hormone
0.00021 - 4.2
juvenile hormone I
0.00034 - 0.0025
juvenile hormone II
0.00003 - 2.4
juvenile hormone III
3.7
methyl farnesoate
-
0.000046 - 0.000641
saturated juvenile hormone III
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
22
Alpha-naphthyl acetate
-
wild-type, at 30C in 50 mM sodium phosphate buffer, pH 7.4, containing 1 mg/mL of bovine serum albumin
0.1 - 0.39
Juvenile hormone
0.618 - 1.6
juvenile hormone I
0.013 - 4.5
juvenile hormone III
3
methyl farnesoate
-
0.7 - 3.1
saturated juvenile hormone III
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
53.65
Alpha-naphthyl acetate
-
wild-type, at 30C in 50 mM sodium phosphate buffer, pH 7.4, containing 1 mg/mL of bovine serum albumin
22000 - 53000
juvenile hormone III
4800 - 14000
saturated juvenile hormone III
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0016
1,1,1-trifluoro-3-(octane-1-sulfonyl)propan-2,2-diol
Tenebrio molitor
Q8WRD8
IC50: 0.0016 mM
0.00000099 - 0.0000036
1,1,1-trifluoro-3-decylsulfanyl-propan-2-one
0.0000022 - 0.000012
1,1,1-trifluoro-3-dodecylsulfanyl-propan-2-one
0.000014 - 0.0017
1,1,1-trifluoro-3-hexylsulfanyl-propan-2-one
0.0000046 - 0.0000091
1,1,1-trifluoro-3-octylsulfanyl-propan-2-one
0.000036 - 0.0004
1,1,1-trifluoro-3-phenethylsulfanyl-propan-2-one
0.000022 - 0.00033
3-Octylthio-1,1,1-trifluoropropan-2-one
0.1
Carbaryl
Tenebrio molitor
Q8WRD8
weak, IC50: above 0.1 mM
1
methamidophos
Tenebrio molitor
Q8WRD8
weak, IC50: above 1 mM
0.0084
O-ethyl-S-phenyl-phosphoramidothiolate
Tenebrio molitor
Q8WRD8
IC50: 0.0084 mM
0.24
Phenylmethylsulfonylfluoride
Tenebrio molitor
Q8WRD8
mediocre inhibitor, IC50: 0.24 mM
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0124
-
pH 8, fourth larval instar, at day 9 after molt
0.0135
-
pH 8, highest specific activity in the fourth larval instar, at day 3 after molt
0.573
-
juvenile hormone III
0.9
recombinant JHE, hydrolysis of alpha-naphthyl acetate
1.1
recombinant JHE, hydrolysis of juvenile hormone III
1.841
-
pH 8, purified JHE from the fourth larval instar, at day 3 after molt
5.3
recombinant JHE, hydrolysis of p-nitrophenyl acetate
15.1
recombinant JHE, hydrolysis of HEPTAT
350
-
purified JHE from pupa, pH 8, 30C
500000
-
purified wild-type, at 30C in 50 mM sodium phosphate buffer, pH 7.4, containing 0.0005 mM juvenile hormone III and 1 mg/mL of bovine serum albumin
1050000
-
purified wild-type, at 30C in 50 mM sodium phosphate buffer, pH 7.4, containing 0.0005 mM juvenile hormone III diol and 1 mg/mL of bovine serum albumin
2370000
-
purified mutant F259I, at 30C in 50 mM sodium phosphate buffer, pH 7.4, containing 0.0005 mM juvenile hormone III and 1 mg/mL of bovine serum albumin
4200000
-
purified mutant T314V, at 30C in 50 mM sodium phosphate buffer, pH 7.4, containing 0.0005 mM juvenile hormone III and 1 mg/mL of bovine serum albumin
4880000
-
purified mutant T314V, at 30C in 50 mM sodium phosphate buffer, pH 7.4, containing 0.0005 mM juvenile hormone III diol and 1 mg/mL of bovine serum albumin
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 9.5
-
juvenile hormone I
7.1
-
assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
the activity decreases significantly below pH 5
5.2 - 10.8
active at a wide range of pH, at least 80% of the maximum activity in the pH-range
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22
assay at; assay at
35
-
assay at
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.9
recombinant JHE, isoelectric focusing, pH-gradient: pH 3-7
5.1
-
two isoelectric forms, isoelectric focusing
5.5
-
two enzyme forms, minor form, isoelectric focusing, pH-gradient of 4-6.5
5.6 - 5.9
-
two enzyme forms, major form, isoelectric focusing, pH-gradient of 4-6.5
6
-
isoelectric focusing
6.13
theoretical
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
whole body
Manually annotated by BRENDA team
-
of honey bee female castes, enzyme expression
Manually annotated by BRENDA team
-
highest activity
Manually annotated by BRENDA team
-
dorsal abdominal epidermis of the last instar larvae
Manually annotated by BRENDA team
strong expression of Bmcce-5; strong expression of Bmcce-5
Manually annotated by BRENDA team
strong expression of Bmcce-5; strong expression of Bmcce-5
Manually annotated by BRENDA team
-
pharate pupae
Manually annotated by BRENDA team
-
JHE is prominent only on the final day of 5th instar nymph
Manually annotated by BRENDA team
-
the highest levels of activity in day 2 virgin and mated females is found in ovaries followed by the carcass and then hemolymph. For ovary the activity is highest in virgin compared to mated females
Manually annotated by BRENDA team
-
moulting fluid
Manually annotated by BRENDA team
-
the highest levels of activity in day 2 virgin and mated females is found in ovaries followed by the carcass and then hemolymph
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
recombinant JHE
-
Manually annotated by BRENDA team
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
48000
-
Trichoplusia ni, gel filtration, gel electrophoresis
57000
-
2 * 57000, major form with pI 5.6, the subunits are not linked by disulfide bridges, SDS-PAGE
60000
-
Galleria mellonella
61012
-
x * 61012, Heliothis virescens, deduced from nucleotide sequence
62000
-
Lymantria dispar
63600
1 * 66000, recombinant JHE, SDS-PAGE, 1 * 63600, recombinant JHE, sequence calculation
120000
-
major form with pI 5.6, nondenaturing PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 57000, major form with pI 5.6, the subunits are not linked by disulfide bridges, SDS-PAGE
monomer
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
no glycoprotein
-
-
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging drop method, 2.7 A crystal structure of the enzyme in complex with the transition state analogue inhibitor 3-octylthio-1,1,1-trifluoropropan-2-one covalently bound to the active site; in complex with 3-octylthio-1,1,1-trifluoropropan-2-one, hanging drop vapor diffusion method
recombinant JHE, complexed with 1,1,1-trifluoro-3-octylsulfanyl-propan-2-one
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 8.5
-
5C, 14 h stable
23478
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
-
pH 6.5-8.5, 14 h stable
60.4 - 61.8
-
5 min, 50% loss of activity, high- and low-selected JHE activity lines
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
Unstable when exposed to charged environments of low enzyme concentration
-
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acetone
Ethanol
isopropanol
Triton X-100
additional information
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, stable for 2 months
-
-70C, stable
-
5C, pH 6.5-8.5, 14 h stable
-
stable in 40% sucrose for 5 months
-
storage on ice, 0.1 M phosphate buffer, pH 7.1, up to 3 weeks, stable
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
147fold, from the fourth larval instar, at day 3 after molt
-
affinity chromatography with the ligand 3-[(4'-mercapto)butylthio]-1,1,1-trifluoropropan-2-one
-
expressed wild-type and mutant proteins purified by anion-exchange chromatography. Purification efficiencies of wild-type JHE, mutant F259I, and mutant T314V are 77%, 32%, and 51%, respectively and are purified 4-, 4.4-, and 4.5fold, respectively
-
from pupa, 49fold
-
purified preparation reveals 2 distinct bands: the heavy band does not contain any detectable 1-naphthylacetate activity and is comprised of 3 closely positioned protein bands, the second band exhibits 1-naphthylacetate esterase activity in addition to juvenile hormone esterase activity
-
recombinant enzyme secreted into the cell medium in a baculovirus expression system, affinity chromatography with 8-mercapto-1,1,1-trifluoro-2-octanone
recombinant JHE
recombinant JHE, partial
-
recombinant JHE, partial, 2.5fold
recombinant protein
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cloning of an ecdysteroid sensitiv and juvenile hormone non-susceptible juvenile hormone esterase related gene, expression in Escherichia coli strain HT115 (DE3)
EAT43357 cDNA is expressed in a baculovirus system. Proteins isolated from Sf9 cells infected with recombinant baculovirus expressing EAT43357 gene exhibit JHE activity
expressed in the Bombyx mori nondiapause strain pnd-w1
-
expression in High 5 cells
expression in the baculovirus expression system
-
from fat bodies of pupae, full-length cDNA, expression in Hi-5 cells from Trichoplusia ni, sequencing
full-length cDNA, expression in Hi5 cells from Trichoplusia ni
-
gene Bmcce-5, DNA and amino acid sequence determination and analysis, intron-exon structures, sequence comparisons and phylogenetic analysis, recombinant expression of BmCCE-5 as C-terminal V5-His6-tagged protein in Spodoptera frugiperda Sf9 cells using the baculovirus system; gene Bmjhe, DNA and amino acid sequence determination and analysis, intron-exon structures, sequence comparisons and phylogenetic analysis of JHE, recombinant expression of BmJHE as C-terminal V5-His6-tagged protein in Spodoptera frugiperda Sf9 cells using the baculovirus system
gene RfEst1, cloning from gut cDNA library, DNA and amino acid sequence determination and analysis, sequence comparisons, expression analysis
genes Tcjhe-a, Tcjhe-b, Tcjhe-c, Tcjhe-d, and Tcjhe-e, encoding isozymes that vary only in the 5'-UTR sequences, DNA and amino acid sequence determination and analysis, intron-exon structure, phylogenetic analysis, overview, recombinant expression of JHE as C-terminal V5-His6-tagged protein in Spodoptera frugiperda Sf9 cells using the baculovirus system
native and mutant enzyme expressed in High 5 cells
recombinant baculovirus AcMsJHE-F259I expressing mutant F259I, recombinant baculovirus AcMsJHE-T314V or recombinant baculovirus pAcUW21-T314V expressing mutant T314V. AcMsJHE-T314V generated by transfection of Sf-21 cells with pAcUW21-T314V and linearized BacPAK6 baculovirus DNA. Wild-type JHE, mutants F259I and T314V produced in insect High Five cells cultured in ESF 921 medium that are inoculated with AcMsJHE7, AcMsJHE-F259I, or AcMsJHE-T314V, respectively
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the enzyme is rhythmically up-regulated close to each molt during the corn stalk borer's larval development
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
T314V
-
no major changes in the overall structure of the substrate binding pocket. The KM of mutant T314V for juvenile hormone III is 2.9fold higher than that of JHE, whereas kcat is 6.4fold higher. Specific activity of mutant T314V for juvenile hormone III diol is not statistically different than that of wild-type for juvenile hormone III
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
-
the high tolerance of organic solvents may make JHE useful in future applications as a synthetic catalyst