The product is most likely the keto-form of 4-oxalomesaconate (as shown in the reaction) [1,2]. It can be converted to the enol-form, 4-hydroxybuta-1,3-diene-1,2,4-trioate, either spontaneously or by EC 5.3.2.8, 4-oxalomesaconate tautomerase .
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SYSTEMATIC NAME
IUBMB Comments
2-pyrone-4,6-dicarboxylate lactonohydrolase
The product is most likely the keto-form of 4-oxalomesaconate (as shown in the reaction) [1,2]. It can be converted to the enol-form, 4-hydroxybuta-1,3-diene-1,2,4-trioate, either spontaneously or by EC 5.3.2.8, 4-oxalomesaconate tautomerase [3].
the catalytic reaction contains the nucleophilic attack, the cleavage of C-O bond of lactone (substrate) and the intramolecular proton transfer. The intermediate has two intramolecular proton transfer pathways, leading to two final hydrolysis products. According to the energy profile, 4-carboxy-2-hydroxymuconate is the main hydrolysis product, and the isomerization between 4-carboxy-2-hydroxymuconate and 4-oxalomesaconate is suggested to occur in solvent. Residue Asp248 acts as a general base to activate the hydrolytic water molecule. Residues His31, His33 and His180 play assistant roles
the catalytic reaction contains the nucleophilic attack, the cleavage of C-O bond of lactone (substrate) and the intramolecular proton transfer. The intermediate has two intramolecular proton transfer pathways, leading to two final hydrolysis products. According to the energy profile, 4-carboxy-2-hydroxymuconate is the main hydrolysis product, and the isomerization between 4-carboxy-2-hydroxymuconate and 4-oxalomesaconate is suggested to occur in solvent. Residue Asp248 acts as a general base to activate the hydrolytic water molecule. Residues His31, His33 and His180 play assistant roles
monoiodoacetate had essentially no effect, Mg(NO3)2, Ni(NO3)2, Ca(NO3)2, Cr(NO3)3 and Fe(NO3)3 shows essentially no effect, KCN and various metal chelating reagents including EDTA, tiron, 1,10-phenanthroline, 2,2'-bipyridine, diethyldithiocarbonate and 8-hydroxyquinoline shows no significant effect
proposed catabolic pathway for PCA by Comamonas sp. strain E6 and gene organization of the PCA45 pathway genes, involvement of orf1 harbouring gene pmdD in the PCA45 pathway, overview
proposed catabolic pathway for PCA by Comamonas sp. strain E6 and gene organization of the PCA45 pathway genes, involvement of orf1 harbouring gene pmdD in the PCA45 pathway, overview
disruption of gene pmdD in E6, which encodes PDC hydrolase, results in a growth defect on protocatechuate, indicating that these gene is essential for the growth of E6 on protocatechuate
disruption of gene pmdD in E6, which encodes PDC hydrolase, results in a growth defect on protocatechuate, indicating that these gene is essential for the growth of E6 on protocatechuate
disruption of gene pmdD in E6, which encodes PDC hydrolase, resultes in a growth defect on protocatechuate, indicating that these gene is essential for the growth of E6 on protocatechuate
disruption of gene pmdD in E6, which encodes PDC hydrolase, resultes in a growth defect on protocatechuate, indicating that these gene is essential for the growth of E6 on protocatechuate
gene pmdD, DNA and amino acid sequence determination and analysis, the pmdUKEFDABC genes constitute an operon, the transcription start site of the pmd operon is mapped at 167 nucleotides upstream of the initiation codon of pmdU. Quantitative real-time RT-PCR expression analysis
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
pmd promoter activity is enhanced 20fold when the cells grow in presence of protocatechuate. Inducers of the pmd operon are protocatechuic acid and 2-pyrone-4,6-dicarboxylate, but 2-pyrone-4,6-dicarboxylate is the more effective inducer
pmd promoter activity is enhanced 20fold when the cells grow in presence of protocatechuate. Inducers of the pmd operon are protocatechuic acid and 2-pyrone-4,6-dicarboxylate, but 2-pyrone-4,6-dicarboxylate is the more effective inducer
pmd promoter activity is enhanced 20fold when the cells grow in presence of protocatechuate. Inducers of the pmd operon are protocatechuic acid and 2-pyrone-4,6-dicarboxylate, but 2-pyrone-4,6-dicarboxylate is the more effective inducer