Information on EC 3.1.1.54 - acetoxybutynylbithiophene deacetylase

for references in articles please use BRENDA:EC3.1.1.54
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The expected taxonomic range for this enzyme is: Tagetes patula

EC NUMBER
COMMENTARY hide
3.1.1.54
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RECOMMENDED NAME
GeneOntology No.
acetoxybutynylbithiophene deacetylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
5-(4-acetoxybut-1-ynyl)-2,2'-bithiophene + H2O = 5-(4-hydroxybut-1-ynyl)-2,2'-bithiophene + acetate
show the reaction diagram
highly specific; this enzyme is highly specific
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of carboxylic ester
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-
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SYSTEMATIC NAME
IUBMB Comments
5-(4-acetoxybut-1-ynyl)-2,2'-bithiophene O-acetylhydrolase
The enzyme is highly specific.
CAS REGISTRY NUMBER
COMMENTARY hide
82346-63-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5-(4-acetoxybut-1-ynyl)-2,2'-bithiophene + H2O
5-(4-hydroxy-but-1-ynyl)-2,2'-bithiophene + acetate
show the reaction diagram
additional information
?
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none of various acetate esters as well as other naturally occuring esters like chlorogenic acid or p-coumaroyl-CoA are hydrolysed by the enzyme
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
5-(4-acetoxybut-1-ynyl)-2,2'-bithiophene + H2O
5-(4-hydroxy-but-1-ynyl)-2,2'-bithiophene + acetate
show the reaction diagram
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enzyme involved in the transformation of an acetylenic compound
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?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
bovine serum albumin
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BSA forms a weak conjugate with the substrate which inhibits the enzyme competitively
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additional information
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ascorbic acid, KCN at 0.001-100 mM, cysteine, glutathione, dithiothreitol, 5,5'-dithiobis(2-nitrobenzoate), 4-chloromercuribenzoate at 0.001-1 mM are no inhibitors
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
bovine serum albumin
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activity is affected considerably, 5.0 mg/ml prevents substrate polymerization
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.02
5-(4-Acetoxybut-1-ynyl)-2,2'-bithiophene
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pH 8.0, 30°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0156
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
67000
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gel filtration
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
90
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preparation heated for 5 min is inactive
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
after 6 months of repeated thawing and freezing about 50% of the esterase activity disappears
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, purified enzyme can be stored for more than 3 months without a marked loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE