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Information on EC 3.1.1.53 - sialate O-acetylesterase and Organism(s) Mus musculus and UniProt Accession P70665

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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.53 sialate O-acetylesterase
IUBMB Comments
Acts on free and glycosidically bound N-acetyl- or N-glycoloyl-neuraminic acid; acts mainly on the 4-O- and 9-O-acetyl groups. Also acts on some other O-acetyl esters, both cyclic and acyclic compounds, which are not sialic acids.
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This record set is specific for:
Mus musculus
UNIPROT: P70665
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Word Map
The taxonomic range for the selected organisms is: Mus musculus
The enzyme appears in selected viruses and cellular organisms
Synonyms
hemagglutinin-esterase, hemagglutinin esterase, sialate-o-acetylesterase, sialic acid acetylesterase, sialate o-acetylesterase, sialate-o-acetylesterases, siabb1, sialate 9-o-acetylesterase, sialic acid-specific 9-o-acetylesterase, haemagglutinin esterase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
cytosolic sialic acid 9-O-acetylesterase
-
lysosomal sialic acid 9-O-acetylesterase
-
lysosomal sialic acid O-acetylesterase
-
9-O-acetyl sialic acid esterase
-
Siae gene encodes two splice forms
Lse
-
one splice variant
sialate-O-acetylesterase
-
-
sialate: O-acetyl esterase
-
-
sialic acid acetyl esterase
-
-
sialic acid O-acetylesterase
-
-
Sialic acid-specific 9-O-acetylesterase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of carboxylic ester
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
N-acyl-O-acetylneuraminate O-acetylhydrolase
Acts on free and glycosidically bound N-acetyl- or N-glycoloyl-neuraminic acid; acts mainly on the 4-O- and 9-O-acetyl groups. Also acts on some other O-acetyl esters, both cyclic and acyclic compounds, which are not sialic acids.
CAS REGISTRY NUMBER
COMMENTARY hide
89400-31-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
N-acetyl-9-O-acetylneuraminate + H2O
N-acetylneuraminate + acetate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Diethyl-4-nitrophenylphosphate
-
diisopropyl fluorophosphate
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
Uniprot
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
restricted distribution, strongest expression of the lysosomal enzyme
Manually annotated by BRENDA team
hepatoietic cell line
Manually annotated by BRENDA team
restricted distribution, strongest expression of the lysosomal enzyme
Manually annotated by BRENDA team
-
catalytic regulation of B cell receptor (BCR) signalling and B-cell development, knockout mice: striking decrease in number of splenic marginal zone B-cells and marginal zone B-cell precursors, reduced number in follicular B-cells, loss of recirculating B-cells in bone marrow, lymphocyte-intrinsic defects
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
isoform Lse, secreted from post-Golgi vesicle, limited lysosomal localisation
Manually annotated by BRENDA team
additional information
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
mice with a mutation in sialate: O-acetyl esterase (inframe deletion of exon 2, resulting in a protein lacking esterase activity) exhibit enhanced B cell receptor activation, defects in peripheral B cell development, and spontaneously develop antichromatin autoantibodies and glomerular immune complex deposits. The 9-O-acetylation state of sialic acid regulates the function of CD22, a Siglec that functions in vivo as an inhibitor of BCR signaling
physiological function
-
sialic acid acetyl esterase removes acetyl moieties from the 9-OH position of sialic acid, and thus permits alpha2,6 linked sialic acid on N-glycans on B cell glycoproteins to interact with CD22/Siglec-2, a sialic acid binding lectin that can inhibit B cell antigen receptor signaling
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
SIAE_MOUSE
541
0
60775
Swiss-Prot
Secretory Pathway (Reliability: 4)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
250000
nonreducing PAGE, equal MW for both the larger and smaller Lse subunit, expressed in COS-7 cells
75000
x * 75000, nonreducing SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 75000, nonreducing SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
side-chain modification
glycoprotein, Lse
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C196F
-
naturally occuring mutation, the mutant shows highly reduced catalytic activity compared with the wild-type enzyme
Q335P
-
naturally occuring mutation, the mutant shows highly reduced catalytic activity compared with the wild-type enzyme
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in COS-7 cells, both the larger and smaller Lse subunit
genotyping, exome sequencing, in HEK293 T cells overexpressed recombinant enzyme can be secreted by the transfected lymphocytes, in the contrary to the physiological state of the enzyme
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Takematsu, H.; Diaz, S.; Stoddart, A.; Zhang, Y.; Varki, A.
Lysosomal and cytosolic sialic acid 9-O-acetylesterase activities can be encoded by one gene via differential usage of a signal peptide-encoding exon at the N terminus
J. Biol. Chem.
274
25623-25631
1999
Mus musculus (P70665), Mus musculus
Manually annotated by BRENDA team
Guimaraes, M.J.; Bazan, J.F.; Castagnola, J.; Diaz, S.; Copeland, N.G.; Gilbert, D.J.; Jenkins, N.A.; Varki, A.; Zlotnik, A.
Molecular cloning and characterization of lysosomal sialic acid O-acetylesterase
J. Biol. Chem.
271
13697-13705
1996
Rattus norvegicus, Mus musculus (P70665), Mus musculus
Manually annotated by BRENDA team
Cariappa, A.; Takematsu, H.; Liu, H.; Diaz, S.; Haider, K.; Boboila, C.; Kalloo, G.; Connole, M.; Shi, H.N.; Varki, N.; Varki, A.; Pillai, S.
B cell antigen receptor signal strength and peripheral B cell development are regulated by a 9-O-acetyl sialic acid esterase
J. Exp. Med.
206
125-138
2009
Mus musculus
Manually annotated by BRENDA team
Chellappa, V.; Taylor, K.N.; Pedrick, K.; Donado, C.; Netravali, I.A.; Haider, K.; Cariappa, A.; Dalomba, N.F.; Pillai, S.
M89V sialic acid acetyl esterase (SIAE) and all other non-synonymous common variants of this gene are catalytically normal
PLoS ONE
8
e53453
2013
Mus musculus, Homo sapiens (Q9HAT2), Homo sapiens
Manually annotated by BRENDA team