PMA, 100 nM to cells induces translocation (maximum after 15 min) of enzyme to the Golgi apparatus and release of 4-5% of cell-associated arachidonic acid within 80 min, effect impaired by PKC inhibition and block of protein synthesis
10% mouse serum to cells induces immediate translocation (within 60 seconds) of enzyme to the Golgi apparatus and release of 20% of cell-associated arachidonic acid within 80 min, effect depends on ERK and p38/MAPK pathway activation and calcium binding, effect is impaired by block of protein synthesis
Transforming growth factor-beta (TGF-beta) activates cytosolic phospholipase A2alpha (cPLA2alpha)-mediated prostaglandin E2 (PGE)2/EP1 and peroxisome proliferator-activated receptor-gamma (PPAR-gamma)/Smad signaling pathways in human liver cancer cells. A novel mechanism for subversion of TGF-beta-induced mitoinhibition.
transient serum-induced translocation of EGFP-tagged enzyme (7%) in parallel to sharp increase in intracellular calcium (within initial 60 seconds), stable binding dependent on influx of extracellular calcium induced here by phorbol myristate acetate
phosphorylation at S505 increases activity 2fold, phosphorylation state not influenced by serum or phorbol myristate acetate, no impact on calcium-mediated membrane binding, constitutively phosphorylated at S505 in immortalized cells (SV40 transformed)
2fold increased specific activity (independent of phosphatidylinositol 4,5-biphosphate), 78% reduced release of arachidonic acid and increased Golgi-binding in response to serum, PIP2-binding site triple mutant
38% reduced release of arachidonic acid and prolonged accumulation at Golgi apparatus in response to serum and 30% reduced release of arachidonic acid in response to phorbol myristate acetate, phosphorylation site mutant
30% reduced release of arachidonic acid in response to phorbol myristate acetate and 50% in response to serum which is further reduced by ERK inhibition, prolonged accumulation at Golgi apparatus in response to serum, phosphorylation site double mutant