Information on EC 3.1.1.52 - phosphatidylinositol deacylase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.1.1.52
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RECOMMENDED NAME
GeneOntology No.
phosphatidylinositol deacylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
1-phosphatidyl-D-myo-inositol + H2O = 1-acylglycerophosphoinositol + a carboxylate
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of carboxylic ester
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Glycerophospholipid metabolism
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SYSTEMATIC NAME
IUBMB Comments
1-phosphatidyl-D-myo-inositol 2-acylhydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
81604-94-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-acyl-2-arachidonoyoyl-sn-glycerol-3-phosphoinositol + H2O
1-acylglycerophosphoinositol + arachidonic acid
show the reaction diagram
1-acyl-2-arachidonoyoyl-sn-glycerol-3-phosphoinositol + H2O
?
show the reaction diagram
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release of arachidonic acid for subsequent metabolic activity in macrophages
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-
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1-acyl-2-oleoyl-sn-glycerol 3-phosphate + H2O
1-acylglycerol 3-phosphate + oleic acid
show the reaction diagram
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32.6% of 1-acyl-2-oleoyl-sn-glycerol-3-phosphorylinositol
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?
1-acyl-2-oleoyl-sn-glycerol-3-phosphocholine + H2O
1-acylglycerol-3-phosphorylcholine + oleic acid
show the reaction diagram
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62.5% of 1-acyl-2-oleoyl-sn-glycerol-3-phosphorylinositol
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?
1-acyl-2-oleoyl-sn-glycerol-3-phosphoinositol + H2O
1-acylglycerophosphoinositol + oleic acid
show the reaction diagram
1-acyl-2-oleoyl-sn-glycerol-3-phosphorylethanolamine + H2O
1-acylglycerol-3-phosphorylethanolamine + oleic acid
show the reaction diagram
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25.1% of 1-acyl-2-oleoyl-sn-glycerol-3-phosphorylinositol
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?
1-acyl-2-oleoyl-sn-glycerol-3-phosphorylserine + H2O
1-acylglycerol-3-phosphorylserine + oleic acid
show the reaction diagram
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21.51% of 1-acyl-2-oleoyl-sn-glycerol-3-phosphorylinositol
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?
1-acyl-2-palmitoyl-sn-glycerol-3-phosphoinositol + H2O
1-acylglycerophosphoinositol + palmitic acid
show the reaction diagram
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-
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?
1-acyl-2-stearoyl-sn-glycerol-3-phosphoinositol + H2O
1-acylglycerophosphoinositol + stearic acid
show the reaction diagram
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-
-
?
1-palmitoyl-2-arachidonyl-sn-glycero-3-phosphocholine + H2O
arachidonic acid + 1-palmitoyl-sn-glycero-3-phosphocholine
show the reaction diagram
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2 min, 37°C (pre-warmed), in presence of 0.5 mg/ml bovine serum albumin and various concentrations of calcium
quantification of release of radio-labelled arachidonic acid
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
1-acyl-2-arachidonoyoyl-sn-glycerol-3-phosphoinositol + H2O
?
show the reaction diagram
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release of arachidonic acid for subsequent metabolic activity in macrophages
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Mg2+
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Mn2+
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Zn2+
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cetyl triammonium bromide
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stimulation
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octyl glucoside
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stimulation
phorbol myristate acetate
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PMA, 100 nM to cells induces translocation (maximum after 15 min) of enzyme to the Golgi apparatus and release of 4-5% of cell-associated arachidonic acid within 80 min, effect impaired by PKC inhibition and block of protein synthesis
Serum
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10% mouse serum to cells induces immediate translocation (within 60 seconds) of enzyme to the Golgi apparatus and release of 20% of cell-associated arachidonic acid within 80 min, effect depends on ERK and p38/MAPK pathway activation and calcium binding, effect is impaired by block of protein synthesis
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Triton X-100
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stimulation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.52
1-acyl-2-oleoyl-sn-glycerol-3-phosphoinositol
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.21
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mutant K271N/K273N/K274N, from activated platelets
0.23
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wild-type, from activated platelets
0.26
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phoshorylated at residue S515, from activated platelets
0.38
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phoshorylated at residue S505, from activated platelets
0.39
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mutant K488N/K543N/K544N, from activated platelets, in presence of 10 mol% phosphatidylinositol 4,5-biphosphate
0.4
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mutant K488N/K543N/K544N, from activated platelets
0.405
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phosphorylated at residues S505 and S727, from activated platelets
0.68
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mutant K271N/K273N/K274N, from activated platelets, in presence of 10 mol% phosphatidylinositol 4,5-biphosphate
0.742
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0.866
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phoshorylated at residue S515, from activated platelets, in presence of 10 mol% phosphatidylinositol 4,5-biphosphate
1.03
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wild-type, from activated platelets, in presence of 10 mol% phosphatidylinositol 4,5-biphosphate
1.45
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phoshorylated at residue S505, from activated platelets, in presence of 10 mol% phosphatidylinositol 4,5-biphosphate
additional information
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5fold increase in specific activity of activated platelet-derived wild-type and phosphorylated forms in presence of 10 mol% phosphatidylinositol 4,5-biphosphate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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activated
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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isolated from wild-type or knockout mice, subsequently SV40 transformed
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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transient serum-induced translocation of EGFP-tagged enzyme (7%) in parallel to sharp increase in intracellular calcium (within initial 60 seconds), stable binding dependent on influx of extracellular calcium induced here by phorbol myristate acetate
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
18300
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x * 18521, amino acid analysis, x * 18300, SDS-PAGE
18520
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amino acid analysis
18521
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x * 18521, amino acid analysis, x * 18300, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 18521, amino acid analysis, x * 18300, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
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phosphorylation at S505 increases activity 2fold, phosphorylation state not influenced by serum or phorbol myristate acetate, no impact on calcium-mediated membrane binding, constitutively phosphorylated at S505 in immortalized cells (SV40 transformed)
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70
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stable up to
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
glycerol, asolecithin, 2-mecaptoethanol stabilize
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
following expression with C-terminal hexa-His-tag, dephosphorylation with potato acid phosphatase
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
together with EGFP cDNA in AdEasy vector or together with monomeric EYFP or ECFP cDNA in pVQAd5CMVK-NpA shuttle plasmid for adenovirus-mediated infection of lung fibroblasts
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K271N/K273N/K274N
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wild-type activity, half the wild-type increase in activity in presence of phosphatidylinositol 4,5-biphosphate
K43N
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impaired release of arachidonic acid in response to serum and phorbol myristate acetate, no translocation to Golgi apparatus in response to serum, C2-domain mutant, calcium and membrane binding
K488N/K543N/K544N
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2fold increased specific activity (independent of phosphatidylinositol 4,5-biphosphate), 78% reduced release of arachidonic acid and increased Golgi-binding in response to serum, PIP2-binding site triple mutant
K541N/K543N/K544N
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2fold increased specific activity (independent of phosphatidylinositol 4,5-biphosphate)
S505A
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38% reduced release of arachidonic acid and prolonged accumulation at Golgi apparatus in response to serum and 30% reduced release of arachidonic acid in response to phorbol myristate acetate, phosphorylation site mutant
S505A/S727A
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30% reduced release of arachidonic acid in response to phorbol myristate acetate and 50% in response to serum which is further reduced by ERK inhibition, prolonged accumulation at Golgi apparatus in response to serum, phosphorylation site double mutant
S505A/S727A/S515A
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similar response to serum as double mutant S505A/S727A, phosphorylation site triple mutant
S515A
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phosphorylation site mutant
S727A
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similar release of arachidonic acid in response to serum like wild-type enzyme, phosphorylation site mutant