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Information on EC 3.1.1.50 - wax-ester hydrolase
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3.1.1.50 wax-ester hydrolaseIUBMB Comments
Also acts on long-chain acylglycerol, but not diacyl- or triacylglycerols.
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Word Map
The enzyme appears in viruses and cellular organisms
Synonyms
weh, wax-ester hydrolase, wax ester hydrolase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
esterase, jojoba wax
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-
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wax ester hydrolase
WEH
wax ester hydrolase
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-
-
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WEH
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-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
a wax ester + H2O = a long-chain alcohol + a long-chain carboxylate
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-
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of carboxylic ester
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-
-
-
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
wax-ester acylhydrolase
Also acts on long-chain acylglycerol, but not diacyl- or triacylglycerols.
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CAS REGISTRY NUMBER
COMMENTARY
66625-78-3
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
1,3-dipalmitin + H2O
glycerol + palmitic acid
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26% of the activity with glycerol palmitate
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-
?
arachidyl-arachidate + H2O
?
-
47% of the activity with glycerol palmitate
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-
?
butyl palmitate + H2O
butanol + palmitate
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76% of the activity with cholesteryl palmitate
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-
?
decanyl palmitate + H2O
decanol + palmitate
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84% of the activity with cholesteryl palmitate
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-
?
docosanyl palmitate + H2O
docosanol + palmitate
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69% of the activity with cholesteryl palmitate
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-
?
dodecanyl palmitate + H2O
dodecanol + palmitate
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85% of the activity with cholesteryl palmitate
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-
?
eicosanyl palmitate + H2O
eicosanol + palmitate
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72% of the activity with cholesteryl palmitate
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-
?
ethyl palmitate + H2O
ethanol + palmitate
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69% of the activity with cholesteryl palmitate
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-
?
glycerol monolaurate + H2O
glycerol + laurate
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213% of the activity with glycerol palmitate
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-
?
glycerol myristate + H2O
glycerol + myristate
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150% of the activity with glycerol palmitate
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-
?
glycerol stearate + H2O
glycerol + stearate
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58% of the activity with glycerol palmitate
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-
?
hexadecanyl acetate + H2O
hexadecanol + acetate
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3% of the activity with hexadecanyl palmitate
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-
?
hexadecanyl arachidate + H2O
hexadecanol + arachidate
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27% of the activity with hexadecanyl palmitate
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-
?
hexadecanyl behenic acid ester + H2O
hexadecanol + behenic acid
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21% of the activity with hexadecanyl palmitate
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-
?
hexadecanyl butyrate + H2O
hexadecanol + butyrate
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10% of the activity with hexadecanyl palmitate
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-
?
hexadecanyl caprylate + H2O
hexadecanol + caprylate
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25% of the activity with hexadecanyl palmitate
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-
?
hexadecanyl laurate + H2O
hexadecanol + laurate
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32% of the activity with hexadecanyl palmitate
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-
?
hexadecanyl myristate + H2O
hexadecanol + myristate
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36% of the activity with hexadecanyl palmitate
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-
?
hexadecanyl stearate + H2O
hexadecanol + stearate
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48% of the activity with hexadecanyl palmitate
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-
?
octadecanyl palmitate + H2O
octadecanol + palmitate
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72% of the activity with cholesteryl palmitate
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-
?
octanyl palmitate + H2O
octanol + palmitate
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92% of the activity with cholesteryl palmitate
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-
?
palmityl palmitate + H2O
hexadecanol + palmitic acid
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67% of the activity with glycerol palmitate
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-
?
stearyl-stearate + H2O
octadecanol + stearic acid
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84% of the activity with glycerol palmitate
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-
?
tetraeicosanyl palmitate + H2O
tetraeicosanol + palmitate
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61% of the activity with cholesteryl palmitate
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-
?
triolein + H2O
glycerol + oleate
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20% of the activity with glycerol palmitate
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-
?
tripalmitin + H2O
glycerol + palmitic acid
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33% of the activity with glycerol palmitate
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-
?
additional information
?
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the conversion of wax ester to fatty acids in jojoba involves three enzymes: wax ester hydrolase, fatty alcohol oxidase and fatty aldehyde dehydrogenase
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?
hexadecanyl palmitate + H2O
hexadecanol + palmitate
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77% of the activity with cholesteryl palmitate
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-
?
palmityl oleate + H2O
hexadecanol + oleic acid
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4 h, 10°C, in presence or absence of 20 mM bile salt (taurocholate)
reaction stop with chloroform/methanol/toluene mixture
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?
palmityl oleate + H2O
hexadecanol + oleic acid
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4 h, 10°C, in presence or absence of 20 mM bile salt (taurocholate)
reaction stop with chloroform/methanol/toluene mixture
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
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the conversion of wax ester to fatty acids in jojoba involves three enzymes: wax ester hydrolase, fatty alcohol oxidase and fatty aldehyde dehydrogenase
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-
?
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
cholate
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activity is dependent on the presence of primary bile salts such as cholate, taurocholate and taurochenodeoxycholate
taurochenodeoxycholate
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activity is dependent on the presence of primary bile salts such as cholate, taurocholate and taurochenodeoxycholate
taurocholate
-
activity is dependent on the presence of primary bile salts such as cholate, taurocholate and taurochenodeoxycholate
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Anemia
Haemochromatosis: novel gene discovery and the molecular pathophysiology of iron metabolism.
Neoplasm Metastasis
Prognostication of uveal melanoma is simple and highly predictive using The Cancer Genome Atlas (TCGA) classification: A review.
Neoplasms
Walleye retroviruses associated with skin tumors and hyperplasias encode cyclin D homologs.
Obesity
Water extract of Houttuynia cordata Thunb. leaves exerts anti-obesity effects by inhibiting fatty acid and glycerol absorption.
Sarcoma
Two closely related but distinct retroviruses are associated with walleye discrete epidermal hyperplasia.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.000625
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crude mid-gut extracts, desalted, in presence of 20 mM taurocholate
0.00063
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crude mid-gut extracts, desalted, in presence of 20 mM taurocholate and trypsin inhibitor
additional information
additional information
-
linearly increasing activity with increasing protein concentration up to 200 microgram protein in the assay
additional information
-
linearly increasing activity with increasing substrate concentration, steep increase up to 125 microM oleic acid
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.5 - 11
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pH 6.5: about 35% of maximal activity, pH 11: about 20% of maximal activity
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
3 - 30
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3°C: about 55% of maximal activity, 30°C: about 30% of maximal activity
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
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activity increases drastically during germination parallel to the development of the gluconeogenic process
brenda
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crude intestine extract, mid-gut, 10-12 h after feeding
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
40
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
activity measurable after 4 h at 10C
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kalinowska, M.; Wojciechowski, Z.A.
Characterization of wax-ester hydrolase from roots of white mustard (Sinapis alba L.) seedlings
Acta Biochim. Pol.
32
263-269
1985
Sinapis alba
-
brenda
Huang, A.H.C.; Moreau, R.A.; Liu, K.D.F.
Development and properties of a wax ester hydrolase in the cotyledons of jojoba seedlings
Plant Physiol.
61
339-341
1978
Simmondsia chinensis
brenda
Moreau, R.A.; Huang, A.H.C.
Enzymes of wax ester catabolism in jojoba
Methods Enzymol.
71
804-813
1981
Simmondsia chinensis
-
brenda
Mankura, M.; Kayama, M.; Saito, S.
Wax ester hydrolysis by lipolytic enzymes in pyloric caeca of various fishes
Bull. Jpn. Soc. Sci. Fish.
50
2127-2131
1984
Acanthopagrus schlegelii, Pseudopleuronectes herzensteini, Nibea mitsukurii, Pagrus major, Paralichthys olivaceus, Scomber japonicus, Seriola quinqueradiata
-
brenda
Tocher, D.R.; Sargent, J.R.
Studies on triacylglycerol, wax ester and sterol ester hydrolases in intestinal caeca of rainbow trout (Salmo gairdneri) fed diets rich in triacylglycerols and wax esters
Comp. Biochem. Physiol. B
77
561-571
1984
Oncorhynchus mykiss
-
brenda
Brahimi-Horn, M.C.; Guglielmino, M.L.; Sparrow, L.G.
Wax esterase in a commercially available source of lipase from Candida cylindracea
J. Biotechnol.
12
299-306
1989
Limtongozyma cylindracea
-
brenda
Bogevik, A.S.; Tocher, D.R.; Waagboe, R.; Olsen, R.E.
Triacylglycerol-, wax ester- and sterol ester-hydrolases in midgut of Atlantic salmon (Salmo salar)
Aquac. Nutr.
14
93-98
2008
Oncorhynchus mykiss, Salmo salar
brenda
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