Information on EC 3.1.1.50 - wax-ester hydrolase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.1.1.50
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RECOMMENDED NAME
GeneOntology No.
wax-ester hydrolase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a wax ester + H2O = a long-chain alcohol + a long-chain carboxylate
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of carboxylic ester
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Cutin, suberine and wax biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
wax-ester acylhydrolase
Also acts on long-chain acylglycerol, but not diacyl- or triacylglycerols.
CAS REGISTRY NUMBER
COMMENTARY hide
66625-78-3
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,3-dipalmitin + H2O
glycerol + palmitic acid
show the reaction diagram
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26% of the activity with glycerol palmitate
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?
arachidyl-arachidate + H2O
?
show the reaction diagram
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47% of the activity with glycerol palmitate
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?
butyryl palmitate + H2O
butanol + palmitate
show the reaction diagram
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76% of the activity with cholesteryl palmitate
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?
cetyl palmitate + H2O
cetyl alcohol + palmitate
show the reaction diagram
cholesteryl palmitate + H2O
cholesterol + palmitate
show the reaction diagram
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-
-
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?
decanyl palmitate + H2O
decanol + palmitate
show the reaction diagram
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84% of the activity with cholesteryl palmitate
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?
docosanyl palmitate + H2O
docosanol + palmitate
show the reaction diagram
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69% of the activity with cholesteryl palmitate
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?
dodecanyl palmitate + H2O
dodecanol + palmitate
show the reaction diagram
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85% of the activity with cholesteryl palmitate
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?
eicosanyl palmitate + H2O
eicosanol + palmitate
show the reaction diagram
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72% of the activity with cholesteryl palmitate
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?
ethyl palmitate + H2O
ethanol + palmitate
show the reaction diagram
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69% of the activity with cholesteryl palmitate
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?
glycerol monolaurate + H2O
glycerol + laurate
show the reaction diagram
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213% of the activity with glycerol palmitate
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?
glycerol myristate + H2O
glycerol + myristate
show the reaction diagram
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150% of the activity with glycerol palmitate
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?
glycerol palmitate + H2O
glycerol + palmitate
show the reaction diagram
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?
glycerol stearate + H2O
glycerol + stearate
show the reaction diagram
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58% of the activity with glycerol palmitate
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?
hexadecanyl acetate + H2O
hexadecanol + acetate
show the reaction diagram
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3% of the activity with hexadecanyl palmitate
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?
hexadecanyl arachidate + H2O
hexadecanol + arachidate
show the reaction diagram
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27% of the activity with hexadecanyl palmitate
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?
hexadecanyl behenic acid ester + H2O
hexadecanol + behenic acid
show the reaction diagram
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21% of the activity with hexadecanyl palmitate
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?
hexadecanyl butyrate + H2O
hexadecanol + butyrate
show the reaction diagram
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10% of the activity with hexadecanyl palmitate
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?
hexadecanyl caprylate + H2O
hexadecanol + caprylate
show the reaction diagram
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25% of the activity with hexadecanyl palmitate
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?
hexadecanyl laurate + H2O
hexadecanol + laurate
show the reaction diagram
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32% of the activity with hexadecanyl palmitate
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?
hexadecanyl myristate + H2O
hexadecanol + myristate
show the reaction diagram
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36% of the activity with hexadecanyl palmitate
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?
hexadecanyl palmitate + H2O
hexadecanol + palmitate
show the reaction diagram
hexadecanyl stearate + H2O
hexadecanol + stearate
show the reaction diagram
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48% of the activity with hexadecanyl palmitate
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?
myristyl myristate + H2O
tetradecanol + myristate
show the reaction diagram
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?
N-methylindoxyl myristate + H2O
?
show the reaction diagram
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?
octadecanyl palmitate + H2O
octadecanol + palmitate
show the reaction diagram
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72% of the activity with cholesteryl palmitate
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?
octanyl palmitate + H2O
octanol + palmitate
show the reaction diagram
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92% of the activity with cholesteryl palmitate
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?
palmityl oleate + H2O
hexadecanol + oleate
show the reaction diagram
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?
palmityl oleate + H2O
hexadecanol + oleic acid
show the reaction diagram
palmityl palmitate + H2O
hexadecanol + palmitic acid
show the reaction diagram
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67% of the activity with glycerol palmitate
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?
stearyl laurate + H2O
octadecanol + lauric acid
show the reaction diagram
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?
stearyl-stearate + H2O
octadecanol + stearic acid
show the reaction diagram
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84% of the activity with glycerol palmitate
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?
tetraeicosanyl palmitate + H2O
tetraeicosanol + palmitate
show the reaction diagram
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61% of the activity with cholesteryl palmitate
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?
triolein + H2O
glycerol + oleate
show the reaction diagram
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20% of the activity with glycerol palmitate
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?
tripalmitin + H2O
glycerol + palmitic acid
show the reaction diagram
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33% of the activity with glycerol palmitate
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?
additional information
?
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the conversion of wax ester to fatty acids in jojoba involves three enzymes: wax ester hydrolase, fatty alcohol oxidase and fatty aldehyde dehydrogenase
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
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the conversion of wax ester to fatty acids in jojoba involves three enzymes: wax ester hydrolase, fatty alcohol oxidase and fatty aldehyde dehydrogenase
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
PCMB
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0.1 mM, 81% inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cholate
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activity is dependent on the presence of primary bile salts such as cholate, taurocholate and taurochenodeoxycholate
deoxycholate
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DTT
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5 mM, 40% stimulation
heptane
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12.5% heptane required for optimal activity
taurochenodeoxycholate
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activity is dependent on the presence of primary bile salts such as cholate, taurocholate and taurochenodeoxycholate
taurocholate
Triton X-100
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stimulates
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0045
Cholesteryl palmitate
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0.028
hexadecanyl palmitate
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0.093
N-methylindoxyl myristate
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000072
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crude mid-gut extracts, desalted
0.000254
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crude mid-gut extracts
0.000278
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crude mid-gut extracts, desalted
0.000305
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crude mid-gut extracts
0.000625
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crude mid-gut extracts, desalted, in presence of 20 mM taurocholate
0.00063
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crude mid-gut extracts, desalted, in presence of 20 mM taurocholate and trypsin inhibitor
0.000665
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crude mid-gut extracts, desalted, in presence of 20 mM taurocholate
0.074
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additional information
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linearly increasing activity up to 24 h of incubation; linearly increasing activity with increasing protein concentration up to 200 microgram protein in the assay; linearly increasing activity with increasing substrate concentration, steep increase up to 125 microM oleic acid
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.8 - 8.5
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broad optimum without definite maximum peak
7.4 - 7.9
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8.5 - 9
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hydrolysis of N-methylindoxyl myristate
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 9
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activity sharply decreases below pH 5.5. and above pH 9.0
6.5 - 11
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pH 6.5: about 35% of maximal activity, pH 11: about 20% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 30
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3°C: about 55% of maximal activity, 30°C: about 30% of maximal activity
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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seedlings
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20
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steep loss of activity above
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
activity measurable after 4 h at 10°C
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE