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Information on EC 3.1.1.5 - lysophospholipase and Organism(s) Mus musculus and UniProt Accession Q9WTL7

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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.5 lysophospholipase
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This record set is specific for:
Mus musculus
UNIPROT: Q9WTL7 not found.
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Word Map
The taxonomic range for the selected organisms is: Mus musculus
The enzyme appears in selected viruses and cellular organisms
Synonyms
phospholipase a, lysophospholipase, neuropathy target esterase, phospholipase b, lysopld, galectin-10, charcot-leyden crystal protein, lysophospholipase a, lysopl, cpla2gamma, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Charcot-Leyden crystal protein
-
-
-
-
Charcot-Leyden crystal protein homolog
-
-
-
-
CLC
-
-
-
-
eosinophil lysophospholipases
-
-
Galactin-10
-
-
-
-
Galectin-10
-
-
-
-
hLysoPLA
-
-
LCAT-like lysophospholipase
-
-
lecithinase B
-
-
-
-
lecitholipase
-
-
-
-
LLPL
-
-
LPLase
-
-
Lysolecithin acylhydrolase
-
-
-
-
lysolecithinase
-
-
-
-
lysophopholipase L2
-
-
-
-
lysophosphatidase
-
-
-
-
lysophosphatidylcholine hydrolase
-
-
-
-
lysophospholipase
-
-
lysophospholipase 3
-
-
lysophospholipase A1
-
-
-
-
lysoPLA
-
-
NTE-related esterase
-
-
phosphatidase B
-
-
-
-
phospholipase B
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of carboxylic ester
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
2-lysophosphatidylcholine acylhydrolase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9001-85-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-lauroyl-sn-glycero-3-phosphocholine + H2O
lauric acid + sn-glycero-3-phosphocholine
show the reaction diagram
-
-
-
-
?
1-linoleoyl-sn-glycero-3-phosphocholine + H2O
linoleic acid + glycerophosphorylcholine
show the reaction diagram
-
-
-
-
?
1-myristoyl-sn-glycero-3-phosphocholine + H2O
myristic acid + sn-glycero-3-phosphocholine
show the reaction diagram
-
-
-
-
?
1-oleoyl-sn-glycero-3-phosphocholine + H2O
oleic acid + glycerophosphorylcholine
show the reaction diagram
-
-
-
-
?
1-oleoyl-sn-glycero-3-phosphoethanolamine + H2O
oleic acid + glycero-3-phosphoethanolamine
show the reaction diagram
-
-
-
-
?
1-palmitoyl-2-acetyl-sn-glycero-3-phosphocholine + H2O
?
show the reaction diagram
-
-
-
-
?
1-palmitoyl-sn-glycero-3-phosphocholine + H2O
palmitic acid + glycero-3-phosphocholine
show the reaction diagram
-
-
-
-
?
1-palmitoyl-sn-glycero-3-phosphoethanolamine + H2O
palmitic acid + glycero-3-phosphoethanolamine
show the reaction diagram
-
-
-
-
?
1-stearoyl-sn-glycero-3-phosphocholine + H2O
stearic acid + glycerophosphorylcholine
show the reaction diagram
-
-
-
-
?
1-stearoyl-sn-glycero-3-phosphoethanolamine + H2O
stearic acid + glycero-3-phosphoethanolamine
show the reaction diagram
-
-
-
-
?
2-lysophosphatidylcholine + H2O
glycerophosphocholine + a carboxylate
show the reaction diagram
-
-
-
?
2-palmitoyl-sn-glycero-3-phosphocholine + H2O
palmitic acid + glycero-3-phosphocholine
show the reaction diagram
-
-
-
-
?
lysophosphatidylcholine + H2O
glycerophosphorylcholine + unesterified fatty acid
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-lysophosphatidylcholine + H2O
glycerophosphocholine + a carboxylate
show the reaction diagram
-
-
-
?
additional information
?
-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3(cis,cis-7,10)hexadecadienyl-4-hydroxy-2-butenolide
-
-
Ca2+
-
-
chlorpyrifos pentyl oxon
-
0.000076 mM, 50% inhibition
Co2+
-
-
Cu2+
-
-
dichlorvos
-
0.000028 mM, 50% inhibition
diphenyl fluorophosphinate
-
2 mM, 52% inhibition
EPTC sulfoxide
-
0.2 mM, 51% inhibition
ethyl octylphosphonofluoridate
-
0.0000018 mM, 50% inhibition
Fe2+
-
-
Fe3+
-
partial
Hg2+
-
-
Insulin
-
insulin reduces NRE mRNA levels in a dose-dependent manner in 3T3-L1 adipocytes
-
isopropyl dodecylphosphonofluoridate
-
0.000002 mM, 50% inhibition
n-dodecyl-benzodioxaphosphorine 2-oxide
-
0.000008 mM, 50% inhibition
phenyl N-benzylcarbamate
-
0.2 mM, 47% inhibition
S-n-octyl-benzodioxaphosphorine 2-oxide
-
0.000005 mM, 50% inhibition
Zn2+
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.022
lysophosphatidylcholine
-
substrate concentration between 0.015-0.2 mM
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.34
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.3
-
activity assay
7.5 - 9
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
activity assay
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
isoform lysophospholipase I only
Manually annotated by BRENDA team
primary cultures of mouse neural cells. PLB activity is present in a variety of cultured mouse neural cell types but that acute loss of this activity is not cytotoxic
Manually annotated by BRENDA team
-
macrophage-like
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
LYPA2_MOUSE
231
0
24794
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
27000
-
x * 27000, lysophospholipase I, SDS-PAGE
28000
-
x * 28000, lysophospholipase II, SDS-PAGE
30000
-
lysophospholipase I, gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8.5
-
unstable below pH 6.0 and above pH 8.5
94401
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, in absence of glycerol, the enzyme loses all of its activity during the freezing and thawing process
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, enzyme concentration: 0.01-0.025 mg/ml, 60-70% glycerol, stable for several months
-
4°C, stable for several weeks
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
cytosolic fractions of COS7 cell extracts containing His-tagged NRE are loaded onto a Q-Sepharose column
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
-
into the vectors pcDNA4HisMax and pEGFP-C1
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
APT1 is released at high levels from RAW264.7 macrophage-like cells into the culture medium after stimulation with lipopolysaccharide, the mRNA level of lysophospholipase II in RAW264.7 cells is much less than APT1 and not changed by lipopolysaccharide treatment
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Toyoda, T.; Sugimoto, H.; Yamashita, S.
Sequence, expression in Escherichia coli, and characterizatiom of lysophospholipase II
Biochim. Biophys. Acta
1437
182-193
1999
Mus musculus
Manually annotated by BRENDA team
Zhang, Y.; Dennis, E.A.
Purification and characterization of a lysophospholipase from a macrophage-like cell line P388D1
J. Biol. Chem.
263
9965-9972
1988
Mus musculus
Manually annotated by BRENDA team
Quistad, G.B.; Casida, J.E.
Lysophospholipase inhibition by organophosphorus toxicants
Toxicol. Appl. Pharmacol.
196
319-326
2004
Mus musculus, Vibrio sp.
Manually annotated by BRENDA team
Taniyama, Y.; Fuse, H.; Satomi, T.; Tozawa, R.; Yasuhara, Y.; Shimakawa, K.; Shibata, S.; Hattori, M.; Nakata, M.; Taketomi, S.
Loss of lysophospholipase 3 increases atherosclerosis in apolipoprotein E-deficient mice
Biochem. Biophys. Res. Commun.
330
104-110
2005
Mus musculus
Manually annotated by BRENDA team
Kwatia, M.A.; Doyle, C.B.; Cho, W.; Enhorning, G.; Ackerman, S.J.
Combined activities of secretory phospholipases and eosinophil lysophospholipases induce pulmonary surfactant dysfunction by phospholipid hydrolysis
J. Allergy Clin. Immunol.
119
838-847
2007
Mus musculus, Homo sapiens (Q05315)
Manually annotated by BRENDA team
Read, D.J.; Langford, L.; Barbour, H.R.; Forshaw, P.J.; Glynn, P.
Phospholipase B activity and organophosphorus compound toxicity in cultured neural cells
Toxicol. Appl. Pharmacol.
219
190-195
2007
Mus musculus (Q3TTY0), Mus musculus
Manually annotated by BRENDA team
Kienesberger, P.C.; Lass, A.; Preiss-Landl, K.; Wolinski, H.; Kohlwein, S.D.; Zimmermann, R.; Zechner, R.
Identification of an Insulin-regulated Lysophospholipase with homology to neuropathy target esterase
J. Biol. Chem.
283
5908-5917
2008
Mus musculus
Manually annotated by BRENDA team
Satou, M.; Nishi, Y.; Yoh, J.; Hattori, Y.; Sugimoto, H.
Identification and characterization of acyl-protein thioesterase 1/lysophospholipase I as a ghrelin deacylation/lysophospholipid hydrolyzing enzyme in fetal bovine serum and conditioned medium
Endocrinology
151
4765-4775
2010
Bos taurus, Homo sapiens, Rattus norvegicus, Mus musculus (Q9WTL7)
Manually annotated by BRENDA team