Information on EC 3.1.1.40 - orsellinate-depside hydrolase

for references in articles please use BRENDA:EC3.1.1.40
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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.1.1.40
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RECOMMENDED NAME
GeneOntology No.
orsellinate-depside hydrolase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
orsellinate depside + H2O = 2 orsellinate
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of carboxylic ester
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-
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SYSTEMATIC NAME
IUBMB Comments
orsellinate-depside hydrolase
The enzyme will only hydrolyse those substrates based on the 2,4-dihydroxy-6-methylbenzoate structure that also have a free hydroxy group ortho to the depside linkage.
CAS REGISTRY NUMBER
COMMENTARY hide
62213-12-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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-
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Manually annotated by BRENDA team
lichen
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
erythrin + H2O
?
show the reaction diagram
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-
-
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?
evernic acid + H2O
orsellinic acid + everninic acid
show the reaction diagram
gyrophoric acid + H2O
orsellinic acid
show the reaction diagram
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-
-
?
lecanoric acid + H2O
orsellinic acid
show the reaction diagram
methyllecanorate + H2O
?
show the reaction diagram
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-
-
-
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
diisopropylfluorophosphate
-
-
orsinol
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noncompetitive
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
7.5
Evernic acid
-
-
0.056
Lecanoric acid
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5
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immobilized enzyme
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
42000
-
gel filtration, ultracentrifugation, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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1 * 42000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
57
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60 min stable, loss of activity if incubated longer
100
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immobilized enzyme, 15 min, no loss of activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
frozen, 6 months, no loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
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depsides and their constitutive untis are used in the perfume industry