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Information on EC 3.1.1.4 - phospholipase A2 and Organism(s) Equus caballus and UniProt Accession O77793

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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.4 phospholipase A2
IUBMB Comments
Also acts on phosphatidylethanolamine, choline plasmalogen and phosphatides, removing the fatty acid attached to the 2-position. Requires Ca2+.
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This record set is specific for:
Equus caballus
UNIPROT: O77793
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The taxonomic range for the selected organisms is: Equus caballus
The enzyme appears in selected viruses and cellular organisms
Synonyms
phospholipase a2, cpla2, spla2, spla(2), prdx6, cytosolic phospholipase a2, crotoxin, pla2s, ipla2, spla2-iia, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
14 kDa phospholipase A2
-
-
-
-
Agkistrotoxin
-
-
-
-
amdI1
-
-
-
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Ammodytin I2
-
-
-
-
APLA
-
-
-
-
APP-D-49
-
-
-
-
ASPLA1
-
-
-
-
ASPLA10
-
-
-
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ASPLA11
-
-
-
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ASPLA12
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-
-
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ASPLA13
-
-
-
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ASPLA14
-
-
-
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ASPLA15
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-
-
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ASPLA16
-
-
-
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ASPLA17
-
-
-
-
ASPLA2
-
-
-
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ASPLA3
-
-
-
-
ASPLA4
-
-
-
-
ASPLA5
-
-
-
-
ASPLA6
-
-
-
-
ASPLA7
-
-
-
-
ASPLA8
-
-
-
-
ASPLA9
-
-
-
-
ATX
-
-
-
-
Basic protein I/II
-
-
-
-
BJ-PLA2
-
-
-
-
BJUPLA2
-
-
-
-
BPI/BPII
-
-
-
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CaI-PLA2
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-
-
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Caudoxin
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-
-
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cPm09
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-
-
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Enhancing factor
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-
-
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GIIC sPLA2
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-
-
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GIID sPLA2
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-
-
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GIIE sPLA2
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-
-
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GIIF sPLA2
-
-
-
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GIII sPLA2
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-
-
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Group IB phospholipase A2
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-
-
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Group IIA phospholipase A2
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-
-
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Group V phospholipase A2
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-
-
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Group VI phospholipase A2
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-
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GVI PLA2
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-
-
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GX sPLA2
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-
-
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GXII sPLA2
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-
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GXIII sPLA2
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-
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iPLA2
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-
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lecithinase A
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-
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MP-III 4R
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-
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Muscarinic inhibitor
-
-
-
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Myotoxin
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-
-
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NAJPLA-2A
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-
-
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NAJPLA-2B
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-
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NAJPLA-2C
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-
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Nigexine
-
-
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Non-pancreatic secretory phospholipase A2
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-
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Notechis 11'2
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-
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Notexin
-
-
-
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NPLA
-
-
-
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NPS-PLA2
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-
-
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OHV A-PLA2
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-
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OHV-APLA2
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-
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pgPLA 1a/pgPLA 2a
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-
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phosphatidase
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phosphatide 2-acylhydrolase
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phosphatidolipase
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Phosphatidylcholine 2-acylhydrolase
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Phosphatidylcholine 2-acylhydrolase GIIC
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Phosphatidylcholine 2-acylhydrolase GIID
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Phosphatidylcholine 2-acylhydrolase GIIE
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Phosphatidylcholine 2-acylhydrolase GIIF
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Phosphatidylcholine 2-acylhydrolase GIII
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Phosphatidylcholine 2-acylhydrolase GX
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Phosphatidylcholine 2-acylhydrolase GXII
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Phosphatidylcholine 2-acylhydrolase GXIII
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phospholipase A
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Phospholipase A2 inhibitor
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pkP5
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PLA2-10
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PLA2-VI
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PLA2-VII
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PLA2IID
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platelet activating factor acetyl hydrolase
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Pt-PLA1
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Pt-PLA2
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Secretory-type PLA, stroma-associated homolog
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sPLA(2)-IID
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-
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sPLA(2)-IIE
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-
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sPLA(2)-IIF
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-
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TMV-K49
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-
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Toxin VI
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Toxin VI:5
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of carboxylic ester
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
phosphatidylcholine 2-acylhydrolase
Also acts on phosphatidylethanolamine, choline plasmalogen and phosphatides, removing the fatty acid attached to the 2-position. Requires Ca2+.
CAS REGISTRY NUMBER
COMMENTARY hide
9001-84-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-palmitoyl-2-arachidonylphosphocholine + H2O
1-palmitoyl-glycerophosphocholine + arachidonic acid
show the reaction diagram
-
-
?
1-palmitoyl-2-arachidonylphosphoethanolamine + H2O
1-palmitoyl-glycerophosphoethanolamine + arachidonic acid
show the reaction diagram
-
-
?
1-stearoyl-2-arachidonylphosphocholine + H2O
1-stearoyl-glycerophosphocholine + stearic acid
show the reaction diagram
-
-
?
1,2-dioctanoyl-sn-glycero-3-phosphocholine + H2O
1-octanoyl-sn-glycero-3-phosphocholine + octanoate
show the reaction diagram
-
-
-
?
phospholipids + H2O
?
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
phospholipids + H2O
?
show the reaction diagram
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
dependent
Ca2+
-
two Ca2+ binding sites, first Ca2+ is strongly bound and essential for enzyme activity, the second binds more weakly and improves the enzymes affinity for lipid-water interfaces
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
arachidonyl trifluoromethyl ketone
inhibits dose-dependently
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0106
1-palmitoyl-2-arachidonylphosphocholine as substrate
0.0156
1-stearoyl-2-arachidonylphosphocholine as substrate
0.0225
1-palmitoyl-2-arachidonylphosphoethanolamine as substrate
6671
-
pH6, micellar 1,2-dioctanoyl-sn-glycero-3-phosphocholine, 0.79 mM Ca2+
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
horse
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PA24A_HORSE
749
0
85454
Swiss-Prot
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
105000
SDS-PAGE
13900
-
amino acid composition analysis
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Donne-Op den Kelder, G.M.; de Haas, G.H.; Egmond, M.R.
Localization of the second calcium ion binding site in porcine and equine phospholipase A2
Biochemistry
22
2470-2478
1983
Bos taurus, Equus caballus, Homo sapiens, Sus scrofa
Manually annotated by BRENDA team
Forsell, P.K.A.L.; Lindberg, A.; Karlsson, S.; Lindgren, J.A.; Claesson, H.E.
Purification, characterization, and cDNA sequencing of cytosolic phospholipase A2 from equine neutrophils
J. Lipid Res.
41
1222-1230
2000
Equus caballus (O77793)
Manually annotated by BRENDA team