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Information on EC 3.1.1.4 - phospholipase A2 and Organism(s) Rattus norvegicus and UniProt Accession O35244
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3.1.1.4 phospholipase A2IUBMB Comments
Also acts on phosphatidylethanolamine, choline plasmalogen and phosphatides, removing the fatty acid attached to the 2-position. Requires Ca2+.
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Word Map
- 3.1.1.4
-
arachidonic
- phospholipid
- prostaglandin
-
cyclooxygenase
-
eicosanoids
-
leukotriene
- necrosis
- indomethacin
- agonist
- lysophosphatidylcholine
- endothelial
- neutrophil
- bromide
- phosphatidylethanolamine
- crotalus
- lipoxygenase
- cox-2
-
phorbol
-
ionophore
- pkc
- inositol
- artery
-
neurotoxic
- quinacrine
-
thromboxane
- peroxiredoxins
-
platelet-activating
- diacylglycerols
- nephropathy
- lysophospholipids
-
bilayer
- phosphatidylinositol
-
prelabeled
- edema
-
prostanoids
- phosphatidylserine
- pertussis
-
interfacial
-
phosphatidic
-
envenom
- glycerophospholipids
-
nordihydroguaiaretic
-
presynaptic
- synovial
- prostacyclin
- viper
-
micelle
- pharmacology
- analysis
- food industry
- biotechnology
-
lipoprotein-associated
- ndga
- industry
- drug development
- zymosan
- medicine
- synthesis
The taxonomic range for the selected organisms is: Rattus norvegicus
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Synonyms
phospholipase a2, cpla2, spla2, spla(2), prdx6, cytosolic phospholipase a2, crotoxin, pla2s, ipla2, spla2-iia, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
14 kDa phospholipase A2
-
-
-
-
Agkistrotoxin
-
-
-
-
amdI1
-
-
-
-
Ammodytin I2
-
-
-
-
APLA
-
-
-
-
APP-D-49
-
-
-
-
ASPLA1
-
-
-
-
ASPLA10
-
-
-
-
ASPLA11
-
-
-
-
ASPLA12
-
-
-
-
ASPLA13
-
-
-
-
ASPLA14
-
-
-
-
ASPLA15
-
-
-
-
ASPLA16
-
-
-
-
ASPLA17
-
-
-
-
ASPLA2
-
-
-
-
ASPLA3
-
-
-
-
ASPLA4
-
-
-
-
ASPLA5
-
-
-
-
ASPLA6
-
-
-
-
ASPLA7
-
-
-
-
ASPLA8
-
-
-
-
ASPLA9
-
-
-
-
ATX
-
-
-
-
Basic protein I/II
-
-
-
-
BJ-PLA2
-
-
-
-
BJUPLA2
-
-
-
-
BPI/BPII
-
-
-
-
CaI-PLA2
-
-
-
-
Caudoxin
-
-
-
-
cPm09
-
-
-
-
Enhancing factor
-
-
-
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GIIC sPLA2
-
-
-
-
GIID sPLA2
-
-
-
-
GIIE sPLA2
-
-
-
-
GIIF sPLA2
-
-
-
-
GIII sPLA2
-
-
-
-
Group IB phospholipase A2
-
-
-
-
Group IIA phospholipase A2
-
-
-
-
Group V phospholipase A2
-
-
-
-
Group VI phospholipase A2
-
-
-
-
GVI PLA2
-
-
-
-
GX sPLA2
-
-
-
-
GXII sPLA2
-
-
-
-
GXIII sPLA2
-
-
-
-
iPLA2
lecithinase A
-
-
-
-
MP-III 4R
-
-
-
-
Muscarinic inhibitor
-
-
-
-
Myotoxin
-
-
-
-
NAJPLA-2A
-
-
-
-
NAJPLA-2B
-
-
-
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NAJPLA-2C
-
-
-
-
Nigexine
-
-
-
-
Non-pancreatic secretory phospholipase A2
-
-
-
-
Notechis 11'2
-
-
-
-
Notexin
-
-
-
-
NPLA
-
-
-
-
NPS-PLA2
-
-
-
-
OHV A-PLA2
-
-
-
-
OHV-APLA2
-
-
-
-
pgPLA 1a/pgPLA 2a
-
-
-
-
phosphatidase
-
-
-
-
phosphatide 2-acylhydrolase
-
-
-
-
phosphatidolipase
-
-
-
-
Phosphatidylcholine 2-acylhydrolase
-
-
-
-
Phosphatidylcholine 2-acylhydrolase GIIC
-
-
-
-
Phosphatidylcholine 2-acylhydrolase GIID
-
-
-
-
Phosphatidylcholine 2-acylhydrolase GIIE
-
-
-
-
Phosphatidylcholine 2-acylhydrolase GIIF
-
-
-
-
Phosphatidylcholine 2-acylhydrolase GIII
-
-
-
-
Phosphatidylcholine 2-acylhydrolase GX
-
-
-
-
Phosphatidylcholine 2-acylhydrolase GXII
-
-
-
-
Phosphatidylcholine 2-acylhydrolase GXIII
-
-
-
-
phospholipase A
-
-
-
-
Phospholipase A2 inhibitor
-
-
-
-
pkP5
-
-
-
-
PLA2-10
-
-
-
-
PLA2-VI
-
-
-
-
PLA2-VII
-
-
-
-
PLA2IID
-
-
-
-
platelet activating factor acetyl hydrolase
-
-
-
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Pt-PLA1
-
-
-
-
Pt-PLA2
-
-
-
-
Secretory-type PLA, stroma-associated homolog
-
-
-
-
sPLA(2)-IID
-
-
-
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sPLA(2)-IIE
-
-
-
-
sPLA(2)-IIF
-
-
-
-
TMV-K49
-
-
-
-
Toxin VI
-
-
-
-
Toxin VI:5
-
-
-
-
iPLA2
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of carboxylic ester
-
-
-
-
PATHWAY SOURCE
PATHWAYS
BRENDA
KEGG
-
-, -, -, -, -, -, -, -, -, -, -, -, -, -, -, -, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
phosphatidylcholine 2-acylhydrolase
Also acts on phosphatidylethanolamine, choline plasmalogen and phosphatides, removing the fatty acid attached to the 2-position. Requires Ca2+.
CAS REGISTRY NUMBER
COMMENTARY
9001-84-7
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
1,2-dipalmitoyl-sn-phosphatidylcholine + H2O
1-palmitoyl-sn-phosphatidylcholine + palmitate
the catalytic site for aiPLA2 activity is an S32-H26-D140 triad
-
-
?
1,2-dilinoleoyl-phosphatidylcholine + H2O
1-linoleoyl-glycerophosphorylcholine + linoleic acid
-
-
-
-
?
1,2-dilinoleoyl-phosphatidylcholine + H2O
1-linoleoyl-sn-glycero-3-phosphocholine + linoleic acid
-
-
-
-
?
1,2-dioleoyl-sn-phosphatidylethanolamine + H2O
1-oleoyl-sn-phosphatidylethanolamine + oleic acid
-
-
-
?
1,2-dipalmitoyl-phosphatidylcholine + H2O
1-palmitoyl-glycerophosphorylcholine + palmitic acid
1,2-dipalmitoyl-sn-glycero-3-phosphorylcholine + H2O
1-palmitoyl-sn-glycero-3-phosphorylcholine + palmitate
-
-
-
-
?
1,2-dipalmitoyl-sn-phosphatidylserine + H2O
1-palmitoyl-sn-phosphatidylserine + palmitic acid
-
-
-
?
1-acyl-2-arachidonoyl-sn-glycero-3-phosphoethanolamine + H2O
1-acyl-sn-glycero-3-phosphoethanolamine + arachidonic acid
1-acyl-2-arachidonoyl-sn-glycerophosphoethanolamine + H2O
1-acyl-sn-glycerophosphoethanolamine + arachidonic acid
-
-
-
?
1-palmitoyl-2-arachidonoylphosphatidylcholine + H2O
1-palmitoyl-glycerophosphorylcholine + arachidonic acid
1-palmitoyl-2-arachidonyl-phosphatidylcholine + H2O
1-palmitoyl-sn-glycero-3-phosphocholine + arachidonic acid
-
-
-
-
?
1-palmitoyl-2-arachidonyl-sn-glycero-3-phosphocholine + H2O
1-palmitoyl-sn-glycero-3-phosphorylcholine + arachidonic acid
-
-
-
-
?
1-palmitoyl-2-linoleoyl-phosphatidic acid + H2O
1-palmitoyl-sn-glycero-3-phosphate + linoleic acid
-
-
-
-
?
1-palmitoyl-2-linoleoyl-phosphatidylcholine + H2O
1-palmitoyl-sn-glycero-3-phosphocholine + linoleic acid
-
-
-
-
?
1-palmitoyl-2-linoleoyl-phosphatidylethanolamine + H2O
1-palmitoyl-sn-glycerophosphoethanolamine + linoleic acid
-
-
-
-
?
1-palmitoyl-2-linoleoyl-phosphatidylinositol + H2O
1-palmitoyl-3-phosphoinositol + linoleic acid
-
-
-
-
?
1-palmitoyl-2-linoleoyl-phosphatidylserine + H2O
1-palmitoyl-sn-glycero-3-phosphoserine + linoleic acid
-
-
-
-
?
1-palmitoyl-2-linoleoylphosphatidylcholine + H2O
1-palmitoyl-glycerophosphorylcholine + linolic acid
1-palmitoyl-2-linoleoylphosphatidylethanolamine + H2O
1-palmitoyl-glycerophosphorylethanolamine + linolic acid
1-palmitoyl-2-oleoylphosphatidylcholine + H2O
1-palmitoyl-glycerophosphocholine + oleic acid
-
data for iPLA2
-
?
1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphoinositol + H2O
1-stearoyl-sn-glycero-3-phosphoinositol + arachidonic acid
-
-
-
?
1-stearoyl-2-arachidonoylphosphatidylcholine + H2O
1-stearoyl-glycerophosphorylcholine + arachidonic acid
arachidonyl phosphatidylethanolamine + H2O
glycerophosphoethanolamine + arachidonic acid
-
-
-
-
?
L-1-acyl-2-arachidonyl-sn-glycerol-3-phosphatidylethanolamine + H2O
?
-
-
-
?
1,2-dipalmitoyl-phosphatidylcholine + H2O
1-palmitoyl-glycerophosphorylcholine + palmitic acid
-
-
-
?
1,2-dipalmitoyl-phosphatidylcholine + H2O
1-palmitoyl-glycerophosphorylcholine + palmitic acid
-
-
-
?
1,2-dipalmitoyl-phosphatidylcholine + H2O
1-palmitoyl-glycerophosphorylcholine + palmitic acid
-
data for iPLA2
-
?
1-acyl-2-arachidonoyl-sn-glycero-3-phosphoethanolamine + H2O
1-acyl-sn-glycero-3-phosphoethanolamine + arachidonic acid
-
-
-
?
1-acyl-2-arachidonoyl-sn-glycero-3-phosphoethanolamine + H2O
1-acyl-sn-glycero-3-phosphoethanolamine + arachidonic acid
-
-
-
?
1-palmitoyl-2-arachidonoylphosphatidylcholine + H2O
1-palmitoyl-glycerophosphorylcholine + arachidonic acid
-
-
-
?
1-palmitoyl-2-arachidonoylphosphatidylcholine + H2O
1-palmitoyl-glycerophosphorylcholine + arachidonic acid
-
,data for iPLA2
-
?
1-palmitoyl-2-linoleoylphosphatidylcholine + H2O
1-palmitoyl-glycerophosphorylcholine + linolic acid
-
-
-
?
1-palmitoyl-2-linoleoylphosphatidylcholine + H2O
1-palmitoyl-glycerophosphorylcholine + linolic acid
-
iPLA2
-
?
1-palmitoyl-2-linoleoylphosphatidylethanolamine + H2O
1-palmitoyl-glycerophosphorylethanolamine + linolic acid
-
-
-
?
1-palmitoyl-2-linoleoylphosphatidylethanolamine + H2O
1-palmitoyl-glycerophosphorylethanolamine + linolic acid
-
-
-
?
1-palmitoyl-2-linoleoylphosphatidylethanolamine + H2O
1-palmitoyl-glycerophosphorylethanolamine + linolic acid
-
-
-
-
?
1-palmitoyl-2-linoleoylphosphatidylethanolamine + H2O
1-palmitoyl-glycerophosphorylethanolamine + linolic acid
-
data for iPLA2
-
?
1-stearoyl-2-arachidonoylphosphatidylcholine + H2O
1-stearoyl-glycerophosphorylcholine + arachidonic acid
-
-
-
?
1-stearoyl-2-arachidonoylphosphatidylcholine + H2O
1-stearoyl-glycerophosphorylcholine + arachidonic acid
-
data for iPLA2
-
?
phosphatidylcholine + H2O
1-acylglycerophosphocholine + fatty acid
-
-
-
?
phosphatidylcholine + H2O
1-acylglycerophosphocholine + fatty acid
-
-
-
?
phosphatidylcholine + H2O
1-acylglycerophosphocholine + fatty acid
-
-
-
?
phosphatidylethanolamine + H2O
1-acylglycerophosphorylethanolamine + fatty acid
-
-
-
?
phosphatidylethanolamine + H2O
1-acylglycerophosphorylethanolamine + fatty acid
-
-
-
?
phosphatidylethanolamine + H2O
1-acylglycerophosphorylethanolamine + fatty acid
-
-
-
?
phosphatidylethanolamine + H2O
1-acylglycerophosphorylethanolamine + fatty acid
-
-
-
?
phospholipids + H2O
?
-
release of arachidonic acid: biosynthesis of leucotrienes, thromboxans and prostaglandins
-
-
?
additional information
?
-
-
increased phospholipase A2 activity and inflammatory response but decreased nerve growth factor expression in the olfactory bulbectomized rat model of depression, the effects are associated with increased interleukin-1beta and prostaglandin E2 in the serum and brain, overview
-
-
?
additional information
?
-
-
peroxiredoxin 6 is a bifunctional protein with both GSH peroxidase and phospholipase A2 activities, and is responsible for the metabolism of lung surfactant phospholipids. Prdx6 has a major role in degradation of internalized dipalmitoyl phosphatidylcholine as well as in the remodeling of PC through the deacylation-reacylation pathway by lung granular pneumocytes, i.e. alveolar epithelial type II cells. Mitrogen-activated protein kinase-mediated phosphorylation of peroxiredoxin 6 regulates its phospholipase A2 activity, overview
-
-
?
additional information
?
-
-
phospholipases A2 catalyze hydrolysis of fatty acids from the sn-2 position of phospholipids
-
-
?
additional information
?
-
-
the phospholipase A2 superfamily consists of many different groups of enzymes that catalyze the hydrolysis of the sn-2 ester bond in a variety of different phospholipids, products of the hydrolysis of the sn-2 ester bond of phospholipid are a free fatty acid and lysophospholipid
-
-
?
additional information
?
-
-
AdPLA is most likely a His-Cys PLA2, His23 and Cys113 are critical for activity of AdPLA, catalytic mechanism, overview. AdPLA has phospholipase activity but lacks acyltransferase activity
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
phospholipids + H2O
?
-
release of arachidonic acid: biosynthesis of leucotrienes, thromboxans and prostaglandins
-
-
?
additional information
?
-
-
increased phospholipase A2 activity and inflammatory response but decreased nerve growth factor expression in the olfactory bulbectomized rat model of depression, the effects are associated with increased interleukin-1beta and prostaglandin E2 in the serum and brain, overview
-
-
?
additional information
?
-
-
peroxiredoxin 6 is a bifunctional protein with both GSH peroxidase and phospholipase A2 activities, and is responsible for the metabolism of lung surfactant phospholipids. Prdx6 has a major role in degradation of internalized dipalmitoyl phosphatidylcholine as well as in the remodeling of PC through the deacylation-reacylation pathway by lung granular pneumocytes, i.e. alveolar epithelial type II cells. Mitrogen-activated protein kinase-mediated phosphorylation of peroxiredoxin 6 regulates its phospholipase A2 activity, overview
-
-
?
additional information
?
-
-
phospholipases A2 catalyze hydrolysis of fatty acids from the sn-2 position of phospholipids
-
-
?
additional information
?
-
-
the phospholipase A2 superfamily consists of many different groups of enzymes that catalyze the hydrolysis of the sn-2 ester bond in a variety of different phospholipids, products of the hydrolysis of the sn-2 ester bond of phospholipid are a free fatty acid and lysophospholipid
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
additional information
Ca2+
-
the adipose-specific phospholipase A2 is activated by Ca2+, but it is not essential for activity
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(6E)-6-(bromomethylene)-3-(1-naphthyl)tetrahydro-2H-pyran-2-one
-
i.e. BEL, bromoenol lactone suicide inhibitor, alkylates Cys-residues
1-hexadecyl-3-trifluoroethylglycero-sn-2-phosphomethanol
-
MJ33, competitive inhibitor
3-[2-[2-(2,2-diphenylethyl)-3,5-dioxo-1,2,4-oxadiazolidin-4-yl]ethoxy]benzoate
-
-
4-(((2E)-4-[1-(diphenylmethyl)-2,4-dioxo-1,4-dihydroquinazolin-3(2H)-yl]but-2-en-1-yl)oxy)benzoic acid
-
-
4-(((2E)-4-[3-(diphenylmethyl)-2,4-dioxo-3,4-dihydroquinazolin-1(2H)-yl]but-2-en-1-yl)oxy)benzoic acid
-
-
4-(((2E)-4-[3-(diphenylmethyl)-2,4-dioxo-7-(1H-1,2,4-triazol-1-yl)-3,4-dihydroquinazolin-1(2H)-yl]but-2-en-1-yl)oxy)benzoic acid
-
-
4-(((2E)-4-[3-(diphenylmethyl)-7-fluoro-2,4-dioxo-3,4-dihydroquinazolin-1(2H)-yl]but-2-en-1-yl)oxy)benzoic acid
-
-
4-(((2E)-4-[3-(diphenylmethyl)-7-methoxy-2,4-dioxo-3,4-dihydroquinazolin-1(2H)-yl]but-2-en-1-yl)oxy)benzoic acid
-
-
4-(((2E)-4-[3-(diphenylmethyl)-7-nitro-2,4-dioxo-3,4-dihydroquinazolin-1(2H)-yl]but-2-en-1-yl)oxy)benzoic acid
-
-
4-(((2E)-4-[7-chloro-3-(diphenylmethyl)-2,4-dioxo-3,4-dihydroquinazolin-1(2H)-yl]but-2-en-1-yl)oxy)benzoic acid
-
-
4-([(2E)-4-[2-(2,2-diphenylethyl)-3,5-dioxo-1,2,4-oxadiazolidin-4-yl]but-2-en-1-yl]oxy)benzoate
-
-
4-([(2E)-4-[3-(diphenylmethyl)-2,4,6-trioxo-5-prop-2-en-1-yl-1,3,5-triazinan-1-yl]but-2-en-1-yl]oxy)benzoic acid
-
-
4-([(2E)-4-[3-(diphenylmethyl)-5-ethyl-2,4,6-trioxo-1,3,5-triazinan-1-yl]but-2-en-1-yl]oxy)benzoic acid
-
-
4-([(2E)-4-[3-benzyl-5-(diphenylmethyl)-2,4,6-trioxo-1,3,5-triazinan-1-yl]but-2-en-1-yl]oxy)benzoic acid
-
-
4-([(2E)-4-[3-butyl-5-(diphenylmethyl)-2,4,6-trioxo-1,3,5-triazinan-1-yl]but-2-en-1-yl]oxy)benzoic acid
-
-
4-([4-[2-(2,2-diphenylethyl)-3,5-dioxo-1,2,4-oxadiazolidin-4-yl]but-2-yn-1-yl]oxy)benzoate
-
-
4-[2-[2-(1-benzyl-2-phenylethyl)-3,5-dioxo-1,2,4-oxadiazolidin-4-yl]ethoxy]benzoate
-
-
4-[2-[2-(2,2-diphenylethyl)-3,5-dioxo-1,2,4-oxadiazolidin-4-yl]ethoxy]-2-hydroxybenzoate
-
-
4-[2-[2-(2,2-diphenylethyl)-3,5-dioxo-1,2,4-oxadiazolidin-4-yl]ethoxy]benzoate
-
-
4-[2-[2-(diphenylmethyl)-3,5-dioxo-1,2,4-oxadiazolidin-4-yl]ethoxy]benzoate
-
-
4-[2-[3-(2,2-diphenylethyl)-5-ethyl-2,4,6-trioxo-1,3,5-triazinan-1-yl]ethoxy]benzoic acid
-
-
4-[2-[3-(diphenylmethyl)-2,4,6-trioxo-5-phenyl-1,3,5-triazinan-1-yl]ethoxy]benzoic acid
-
-
4-[2-[3-(diphenylmethyl)-2,4,6-trioxo-5-prop-2-en-1-yl-1,3,5-triazinan-1-yl]ethoxy]benzoic acid
-
-
4-[2-[3-(diphenylmethyl)-5-ethyl-2,4,6-trioxo-1,3,5-triazinan-1-yl]ethoxy]benzoic acid
-
-
4-[2-[3-benzyl-5-(diphenylmethyl)-2,4,6-trioxo-1,3,5-triazinan-1-yl]ethoxy]benzoic acid
-
-
4-[2-[3-butyl-5-(diphenylmethyl)-2,4,6-trioxo-1,3,5-triazinan-1-yl]ethoxy]benzoic acid
-
-
4-[2-[5-chloro-1-(diphenylmethyl)-2-methyl-1H-indol-3-yl]ethoxy]benzoic acid
-
-
4-[3-[2-(2,2-diphenylethyl)-3,5-dioxo-1,2,4-oxadiazolidin-4-yl]propoxy]benzoate
-
-
4-[4-[2-(2,2-diphenylethyl)-3,5-dioxo-1,2,4-oxadiazolidin-4-yl]butoxy]benzoate
-
-
acteoside
-
phenylethanoid glycoside isolated from Clerodendron trichotomum Thunberg, competitive. Acts on cytosolic Ca2+-dependent phospholipase A2 rather than secretory phospholipase A2
lysophosphatidylcholines
-
inhibitory plasma compounds, profile determination by mass spectroscopy, overview
methyl arachidonyl fluorophosphate
-
i.e. MAFP, an inhibitor of cysteine-dependent phospholipases
methyl-arachidonylfluorophosphonate
-
irreversible inhibitor of cPLA2 and iPLA2. Ser214 site in microtubule-associated protein Tau is hyperphosphorylated upon inhibitor treatment
pure phospholipid vesicles
-
exception: vesicles composed of phosphatidylcholine or phosphatidylinositol 4,5-bisphosphate
-
arachidonyl trifluoromethyl ketone
-
i.e. AACOCF3, a cPLA2-specific inhibitor
additional information
-
inhibition mechanisms of 1,2,4-oxadiazolidin-3,5-diones and 1,3,5-triazin-2,4,6-triones, overview
-
additional information
-
no inhibition by decanoyl lysophosphatidylcholine in vitro
-
additional information
-
no inhibition of AdPLA and sPLA2 by bromoenolactone
-
additional information
-
SB202190 and PD98059 abolish the enzyme activation by phosphorylation through protein kinase C by specific inhibition of PKC
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
12-O-tetradecanoylphorbol-13-acetate
-
a protein kinase C agonist, has no effect on Prdx6 expression but leads to about 75% increase in aiPLA2 activity
additional information
-
the JNK inhibitor has no effect on TPA-induced PLA2 activity, either in the intact cells or cell lysate
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0175
1,2-dilinoleoyl-phosphatidylcholine
-
pH 8.0, 25°C, recombinant enzyme
0.0082
1-palmitoyl-2-arachidonyl-phosphatidylcholine
-
pH 8.0, 25°C, recombinant enzyme
0.0116
1-palmitoyl-2-linoleoyl-phosphatidic acid
-
pH 8.0, 25°C, recombinant enzyme
0.0186
1-palmitoyl-2-linoleoyl-phosphatidylcholine
-
pH 8.0, 25°C, recombinant enzyme
0.005
1-palmitoyl-2-linoleoyl-phosphatidylethanolamine
-
pH 8.0, 25°C, recombinant enzyme
0.0073
1-palmitoyl-2-linoleoyl-phosphatidylserine
-
pH 8.0, 25°C, recombinant enzyme
1.83
L-1-acyl-2-arachidonyl-sn-glycerol-3-phosphatidylethanolamine
-
pH 7.5
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.05
3-[2-[2-(2,2-diphenylethyl)-3,5-dioxo-1,2,4-oxadiazolidin-4-yl]ethoxy]benzoate
Rattus norvegicus
-
above
0.012
4-(((2E)-4-[1-(diphenylmethyl)-2,4-dioxo-1,4-dihydroquinazolin-3(2H)-yl]but-2-en-1-yl)oxy)benzoic acid
Rattus norvegicus
-
whole blood assay
0.014
4-(((2E)-4-[3-(diphenylmethyl)-2,4-dioxo-3,4-dihydroquinazolin-1(2H)-yl]but-2-en-1-yl)oxy)benzoic acid
Rattus norvegicus
-
whole blood assay
0.011
4-(((2E)-4-[3-(diphenylmethyl)-2,4-dioxo-7-(1H-1,2,4-triazol-1-yl)-3,4-dihydroquinazolin-1(2H)-yl]but-2-en-1-yl)oxy)benzoic acid
Rattus norvegicus
-
whole blood assay
0.006
4-(((2E)-4-[3-(diphenylmethyl)-7-fluoro-2,4-dioxo-3,4-dihydroquinazolin-1(2H)-yl]but-2-en-1-yl)oxy)benzoic acid
Rattus norvegicus
-
whole blood assay
0.009
4-(((2E)-4-[3-(diphenylmethyl)-7-methoxy-2,4-dioxo-3,4-dihydroquinazolin-1(2H)-yl]but-2-en-1-yl)oxy)benzoic acid
Rattus norvegicus
-
whole blood assay
0.013
4-(((2E)-4-[3-(diphenylmethyl)-7-nitro-2,4-dioxo-3,4-dihydroquinazolin-1(2H)-yl]but-2-en-1-yl)oxy)benzoic acid
Rattus norvegicus
-
whole blood assay
0.011
4-(((2E)-4-[7-chloro-3-(diphenylmethyl)-2,4-dioxo-3,4-dihydroquinazolin-1(2H)-yl]but-2-en-1-yl)oxy)benzoic acid
Rattus norvegicus
-
whole blood assay
0.05
4-([(2E)-4-[2-(2,2-diphenylethyl)-3,5-dioxo-1,2,4-oxadiazolidin-4-yl]but-2-en-1-yl]oxy)benzoate
Rattus norvegicus
-
above
0.0013
4-([(2E)-4-[3-(diphenylmethyl)-2,4,6-trioxo-5-prop-2-en-1-yl-1,3,5-triazinan-1-yl]but-2-en-1-yl]oxy)benzoic acid
Rattus norvegicus
-
-
0.0016
4-([(2E)-4-[3-(diphenylmethyl)-5-ethyl-2,4,6-trioxo-1,3,5-triazinan-1-yl]but-2-en-1-yl]oxy)benzoic acid
Rattus norvegicus
-
-
0.0013
4-([(2E)-4-[3-benzyl-5-(diphenylmethyl)-2,4,6-trioxo-1,3,5-triazinan-1-yl]but-2-en-1-yl]oxy)benzoic acid
Rattus norvegicus
-
-
0.0024
4-([(2E)-4-[3-butyl-5-(diphenylmethyl)-2,4,6-trioxo-1,3,5-triazinan-1-yl]but-2-en-1-yl]oxy)benzoic acid
Rattus norvegicus
-
-
0.023
4-([4-[2-(2,2-diphenylethyl)-3,5-dioxo-1,2,4-oxadiazolidin-4-yl]but-2-yn-1-yl]oxy)benzoate
Rattus norvegicus
-
-
0.05
4-[2-[2-(1-benzyl-2-phenylethyl)-3,5-dioxo-1,2,4-oxadiazolidin-4-yl]ethoxy]benzoate
Rattus norvegicus
-
above
0.027
4-[2-[2-(2,2-diphenylethyl)-3,5-dioxo-1,2,4-oxadiazolidin-4-yl]ethoxy]-2-hydroxybenzoate
Rattus norvegicus
-
-
0.0067
4-[2-[2-(2,2-diphenylethyl)-3,5-dioxo-1,2,4-oxadiazolidin-4-yl]ethoxy]benzoate
Rattus norvegicus
-
-
0.05
4-[2-[2-(diphenylmethyl)-3,5-dioxo-1,2,4-oxadiazolidin-4-yl]ethoxy]benzoate
Rattus norvegicus
-
above
0.03
4-[2-[3-(2,2-diphenylethyl)-5-ethyl-2,4,6-trioxo-1,3,5-triazinan-1-yl]ethoxy]benzoic acid
Rattus norvegicus
-
-
0.05
4-[2-[3-(diphenylmethyl)-2,4,6-trioxo-5-phenyl-1,3,5-triazinan-1-yl]ethoxy]benzoic acid
Rattus norvegicus
-
above
0.005
4-[2-[3-(diphenylmethyl)-2,4,6-trioxo-5-prop-2-en-1-yl-1,3,5-triazinan-1-yl]ethoxy]benzoic acid
Rattus norvegicus
-
-
0.0075
4-[2-[3-(diphenylmethyl)-5-ethyl-2,4,6-trioxo-1,3,5-triazinan-1-yl]ethoxy]benzoic acid
Rattus norvegicus
-
-
0.0035
4-[2-[3-benzyl-5-(diphenylmethyl)-2,4,6-trioxo-1,3,5-triazinan-1-yl]ethoxy]benzoic acid
Rattus norvegicus
-
-
0.0056
4-[2-[3-butyl-5-(diphenylmethyl)-2,4,6-trioxo-1,3,5-triazinan-1-yl]ethoxy]benzoic acid
Rattus norvegicus
-
-
0.003
4-[2-[5-chloro-1-(diphenylmethyl)-2-methyl-1H-indol-3-yl]ethoxy]benzoic acid
Rattus norvegicus
-
-
0.0124
4-[3-[2-(2,2-diphenylethyl)-3,5-dioxo-1,2,4-oxadiazolidin-4-yl]propoxy]benzoate
Rattus norvegicus
-
-
0.05
4-[4-[2-(2,2-diphenylethyl)-3,5-dioxo-1,2,4-oxadiazolidin-4-yl]butoxy]benzoate
Rattus norvegicus
-
above
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.6
-
iPLA2 in a 30000fold purification, substrate: 1-stearoyl-2-arachidonoylphosphatidylcholine
10
-
iPLA2 in a 30000fold purification, substrate: 1-palmitoyl-2-linoleoylphosphatidylcholine
2
-
iPLA2 in a 30000fold purification, substrate: 1-palmitoyl-2-arachidonoylphosphatidylcholine
3
-
iPLA2 in a 30000fold purification, substrate: 1-palmitoyl-2-oleolylphosphatidylcholine
4.3
-
iPLA2 in a 30000fold purification, substrate: 1,2-dipalmitoyl-phosphatidylcholine
5.9
-
iPLA2 in a 30000fold purification, substrate: 1-palmitoyl-2-linoleoylphosphatidylethanolamine
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
distribution of sPLa2-IIe, sPLA2-V, and sPLA2-X is mainly neuronal with highest abundance in cerebral cortex and hippocampus. Enzymes are differentially induced by kainic acid
-
AdPLA is highly expressed specifically in white adipose tissue and is induced during preadipocyte differentiation into adipocytes, overview
additional information
-
expression profiles of isozymes iPLA2beta, sPLA2alpha, and AdPLA, overview
-
distribution of sPLa2-IIe, sPLA2-V, and sPLA2-X is mainly neuronal with highest abundance in cerebral cortex and hippocampus. Enzymes are differentially induced by kainic acid
-
distribution of sPLa2-IIe, sPLA2-V, and sPLA2-X is mainly neuronal with highest abundance in cerebral cortex and hippocampus. Enzymes are differentially induced by kainic acid
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
the adipose-specific phospholipase A2 AdPLA is membrane-associated
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
the enzyme has important physiological roles in the turnover (synthesis and degradation) of lung surfactant phospholipids, in the repair of peroxidized cell membranes, and in the activation of NADPH oxidase type 2
physiological function
-
PLA2 signaling pathways indirectly influence the phosphorylation state of Tau
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). PRDX6_RAT
224
0
24819
Swiss-Prot
other Location (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
AdPLA contains no characteristic serine lipase motif, i.e. GXSXG or GXSGS
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phosphoprotein
-
the aiPLA2 is activated by phosphorylation, specifically at Thr177, through protein kinase C by about 75%, several active isoforms of Erk and p38 phosphorylate Prdx6 resulting in a 11fold increase in aiPLA2 activity
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
C651A
-
catalytic activity similar to wild-type, inactivation by (6E)-6-(bromomethylene)-3-(1-naphthyl)tetrahydro-2H-pyran-2-one requires higher concentrations than for wild-type
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
60
-
for 10 min at pH 5.0 no loss of activity, at pH 7.0 50% of activity remains, pH 9.0 leads to complete loss of activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
column chromatography, immunoadsorbent column containing group II PLA2-antibodies, 735fold
-
recombinant untagged enzyme by ion-exchange chromatography and gel filtration
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
overexpression of His-tagged and/or fluoresence-tagged phospholipase A2 in COS-7 cells, perinuclear region, membranes and cytosol, or 3T3-L1 preadipocytic cells
-
RENATURED/Commentary
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Yang, H-C.; Mosior, M.; Ni, B.; Dennis, E.A.
Regional distribution, ontogeny, purification, and characterization of the Ca2+-independent phospholipase A2 from rat brain
J. Neurochem.
73
1278-1287
1999
Rattus norvegicus
Larsson Forsell, P.K.A.; Kennedy, B.P.; Claesson, H.E.
The human calcium-independent phospholipase A2 gene
Eur. J. Biochem.
262
575-585
1999
Rattus norvegicus, Homo sapiens (O60733), Homo sapiens
Chen, D.; Dennis, E.A.
Expression and characterization of human group V phospholipase A2
Biochim. Biophys. Acta
1394
57-64
1998
Homo sapiens, Mus musculus, Rattus norvegicus
Oishi, T.; Tamiya-Koizumi, K.; Kudo, I.; Iino, S.; Takagi, K.; Yoshida, Y.
Purification and characterization of nuclear alkaline phospholipase A2 in rat ascites hepatoma cells
FEBS Lett.
394
55-60
1996
Rattus norvegicus
Wang, R.; Dodia, C.R.; Jain, M.K.; Fisher, A.B.
Purification and characterization of a calcium-independent acidic phospholipase A2 from rat lung
Biochem. J.
304
131-137
1994
Rattus norvegicus
Nakatani, Y.; Hara, S.; Murakami, M.; Kudo, I.; Inoue, K.
Characterization of cytosolic phospholipase A2 in rat mastocystoma RBL-2H3
Biol. Pharm. Bull.
17
47-50
1994
Rattus norvegicus
Vignais, P.M.; Colbeau, A.; Ngo-Tri, H.; Pilarska, M.
Characterization and subcellular localization of lipase and phospholipase A activities in rat liver
Lipids
1
117-126
1976
Rattus norvegicus
-
Rahman, Y.E.; Cerny, E.A.; Peraino, C.
Purification and some properties of rat spleen phospholipase A
Biochim. Biophys. Acta
321
526-535
1973
Rattus norvegicus, Sus scrofa
Hanahan, D.J.
Phospholipases
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
5
71-85
1971
Crotalus adamanteus, Crotalus atrox, Rattus norvegicus, Sus scrofa
-
Winstead, M.V.; Balsinde, J.; Dennis, E.A.
Calcium-independent phospholipase A2: structure and function
Biochim. Biophys. Acta
1488
28-39
2000
Homo sapiens, Mammalia, Mus musculus, Rattus norvegicus
Lee, D.; Won, J.H.; Auh, C.K.; Park, Y.M.
Purification and characterization of a cytosolic phospholipase A2 from rat liver
Mol. Cells
16
361-367
2003
Rattus norvegicus
Farooqui, A.A.; Ong, W.Y.; Horrocks, L.A.; Farooqui, T.
Brain cytosolic phospholipase A2: localization, role, and involvement in neurological diseases
Neuroscientist
6
169-180
2000
Bos taurus, Homo sapiens, Mammalia, Rattus norvegicus
-
Song, H.; Ramanadham, S.; Bao, S.; Hsu, F.F.; Turk, J.
A bromoenol lactone suicide substrate inactivates group VIA phospholipase A2 by generating a diffusible bromomethyl keto acid that alkylates cysteine thiols
Biochemistry
45
1061-1073
2006
Rattus norvegicus
Kolko, M.; Christoffersen, N.R.; Barreiro, S.G.; Miller, M.L.; Pizza, A.J.; Bazan, N.G.
Characterization and location of secretory phospholipase A2 groups IIE, V, and X in the rat brain
J. Neurosci. Res.
83
874-882
2006
Rattus norvegicus
Gopalsamy, A.; Yang, H.; Ellingboe, J.W.; McKew, J.C.; Tam, S.; Joseph-McCarthy, D.; Zhang, W.; Shen, M.; Clark, J.D.
1,2,4-Oxadiazolidin-3,5-diones and 1,3,5-triazin-2,4,6-triones as cytosolic phospholipase A2alpha inhibitors
Bioorg. Med. Chem. Lett.
16
2978-2981
2006
Rattus norvegicus
Wu, Y.; Feinstein, S.I.; Manevich, Y.; Chowdhury, I.; Pak, J.H.; Kazi, A.; Dodia, C.; Speicher, D.W.; Fisher, A.B.
Mitrogen-activated protein kinase-mediated phosphorylation of peroxiredoxin 6 regulates its phospholipase A2 activity
Biochem. J.
419
669-679
2009
Homo sapiens, Rattus norvegicus
Burke, J.E.; Dennis, E.A.
Phospholipase A2 biochemistry
Cardiovasc. Drugs Ther.
23
49-59
2009
Apis mellifera, Bitis gabonica, Bos taurus, Crotalus sp., Homo sapiens, Mus musculus, Naja naja, Oryza sativa, Rattus norvegicus, Sus scrofa, Protoparvovirus
Duncan, R.E.; Sarkadi-Nagy, E.; Jaworski, K.; Ahmadian, M.; Sul, H.S.
Identification and functional characterization of adipose-specific phospholipase A2 (AdPLA)
J. Biol. Chem.
283
25428-25436
2008
Rattus norvegicus
Cunningham, T.J.; Yao, L.; Lucena, A.
Product inhibition of secreted phospholipase A2 may explain lysophosphatidylcholines unexpected therapeutic properties
J. Inflamm.
5
17
2008
Rattus norvegicus
Song, C.; Zhang, X.Y.; Manku, M.
Increased phospholipase A2 activity and inflammatory response but decreased nerve growth factor expression in the olfactory bulbectomized rat model of depression: effects of chronic ethyl-eicosapentaenoate treatment
J. Neurosci.
29
14-22
2009
Rattus norvegicus
Kirincich, S.J.; Xiang, J.; Green, N.; Tam, S.; Yang, H.Y.; Shim, J.; Shen, M.W.; Clark, J.D.; McKew, J.C.
Benzhydrylquinazolinediones: novel cytosolic phospholipase A2alpha inhibitors with improved physicochemical properties
Bioorg. Med. Chem.
17
4383-4405
2009
Rattus norvegicus, synthetic construct
De-Paula, V.J.; Schaeffer, E.L.; Talib, L.L.; Gattaz, W.F.; Forlenza, O.V.
Inhibition of phospholipase A2 increases Tau phosphorylation at Ser214 in embryonic rat hippocampal neurons
Prostaglandins Leukot. Essent. Fatty Acids
82
57-60
2010
Rattus norvegicus
Du, Y.R.; Xiao, Y.; Lu, Z.M.; Ding, J.; Xie, F.; Fu, H.; Wang, Y.; Strong, J.A.; Zhang, J.M.; Chen, J.
Melittin activates TRPV1 receptors in primary nociceptive sensory neurons via the phospholipase A2 cascade pathways
Biochem. Biophys. Res. Commun.
408
32-37
2011
Rattus norvegicus
Song, H.S.; Choi, M.Y.; Ko, M.S.; Jeong, J.M.; Kim, Y.H.; Jang, B.H.; Sung, J.H.; Kim, M.G.; Whang, W.K.; Sim, S.S.
Competitive inhibition of cytosolic Ca2+-dependent phospholipase A2 by acteoside in RBL-2H3 cells
Arch. Pharm. Res.
35
905-910
2012
Rattus norvegicus
Fisher, A.B.
The phospholipase A2 activity of peroxiredoxin 6
J. Lipid Res.
59
1132-1147
2018
Trematomus bernacchii, Xenopus tropicalis (B1WAZ6), Mus musculus (O08709), Rattus norvegicus (O35244), Bos taurus (O77834), Homo sapiens (P30041), Gallus gallus (Q5ZJF4), Arabidopsis thaliana (Q8S8N6)
EXTERNAL LINKS (specific for EC-Number 3.1.1.4)
Transporter Classification Database (TCDB): 1.N.2.1.6, 1.B.84.1.6, 1.B.84.1.5, 1.B.84.1.3, 1.B.84.1.1, 1.B.84.1.4, 1.B.84.1.2
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