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Information on EC 3.1.1.32 - phospholipase A1 and Organism(s) Arabidopsis thaliana and UniProt Accession Q93V61

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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.32 phospholipase A1
IUBMB Comments
This enzyme has a much broader specificity than EC 3.1.1.4 phospholipase A2. Requires Ca2+.
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Select one or more organisms in this record: ?
This record set is specific for:
Arabidopsis thaliana
UNIPROT: Q93V61
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
phospholipase a1, pla1, ompla, h-rev107, ddhd1, outer membrane phospholipase a, pla1a, lipase h, ves v 1, ipla-1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Allergen Dol m 1.01
-
-
-
-
Allergen Dol m 1.02
-
-
-
-
OM PLA
-
-
-
-
OMPLA
-
-
-
-
Phosphatidylcholine 1-acylhydrolase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of carboxylic ester
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
phosphatidylcholine 1-acylhydrolase
This enzyme has a much broader specificity than EC 3.1.1.4 phospholipase A2. Requires Ca2+.
CAS REGISTRY NUMBER
COMMENTARY hide
9043-29-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-myristoyl 2-oleyl phosphatidylcholine + H2O
2-oleylglycerophosphocholine + myristoate
show the reaction diagram
-
-
-
?
lysophosphatidylcholine + H2O
?
show the reaction diagram
weak substrate
-
-
?
phosphatidic acid + H2O
2-acylglycerol-3-phosphate + a carboxylate
show the reaction diagram
-
-
-
?
phosphatidylethanolamine + H2O
2-acylglycerophosphoethanolamine + a carboxylate
show the reaction diagram
-
-
-
?
1-palmitoyl-2-linoleoyl-phosphatidyl choline + H2O
2-linoleoyl-lysophosphatidyl choline + palmitic acid
show the reaction diagram
-
-
?
phosphatidylcholine + H2O
2-acylglycerophosphocholine + a carboxylate
show the reaction diagram
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
phosphatidylcholine + H2O
2-acylglycerophosphocholine + a carboxylate
show the reaction diagram
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
expression in Saccharomyces cerevisiae
Swissprot
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
upper parts of stamen filaments in middle-stage flower buds
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
associated with microsomal membrane
-
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
LCAT3_ARATH
447
0
50349
Swiss-Prot
other Location (Reliability: 2)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D384A
less than 10% of wild-type activity
H409L
less than 10% of wild-type activity
S177A
less than 10% of wild-type activity
T352A
about 70% of wild-type activity
Y346F
about 30% of wild-type activity
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ishiguro, S.; Kawai-Oda, A.; Ueda, J.; Nishida, I.; Okada, K.
The DEFECTIVE IN ANTHER DEHISCIENCE gene encodes a novel phospholipase A1 catalyzing the initial step of jasmonic acid biosynthesis, which synchronizes pollen maturation, anther dehiscence, and flower opening in Arabidopsis
Plant Cell
13
2191-2209
2001
Arabidopsis thaliana (Q948R1)
Manually annotated by BRENDA team
Noiriel, A.; Benveniste, P.; Banas, A.; Stymne, S.; Bouvier-Nave, P.
Expression in yeast of a novel phospholipase A1 cDNA from Arabidopsis thaliana
Eur. J. Biochem.
271
3752-3764
2004
Arabidopsis thaliana (Q93V61), Arabidopsis thaliana
Manually annotated by BRENDA team