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Information on EC 3.1.1.32 - phospholipase A1 and Organism(s) Homo sapiens and UniProt Accession Q6XZB0

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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.32 phospholipase A1
IUBMB Comments
This enzyme has a much broader specificity than EC 3.1.1.4 phospholipase A2. Requires Ca2+.
Specify your search results
Select one or more organisms in this record:
This record set is specific for:
Homo sapiens
UNIPROT: Q6XZB0
Word Map
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
The taxonomic range for the selected organisms is: Homo sapiens
Synonyms
Allergen Dol m 1.01, Allergen Dol m 1.02, AtLCAT3, BveA, DAD1, DDHD1, DDL1, endothelial lipase, H-Rev107, hepatic lipase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Allergen Dol m 1.01
-
-
-
-
Allergen Dol m 1.02
-
-
-
-
DDHD1
312551
-
H-Rev107
247
-
hepatic lipase
247
-
LIPI
247
-
membrane-associated phosphatidic acid-selective phospholipase A1
274580
-
mPA-PLA1
274580
-
mPA-PLA1alpha
247
-
mPA-PLA1beta
OM PLA
-
-
-
-
OMPLA
-
-
-
-
PA-PLA1
247
-
PA-PLA1/DDHD1
247
-
pancreatic lipase-associated protein 2
247
-
pancreatic lipase-related protein 2
247
-
phosphatidic acid-preferring phospholipase A1
247
-
phosphatidic acid-preferring phospholipase A1/DDHD1
247
-
phosphatidic acid-selective phospholipase A
274578
-
Phosphatidylcholine 1-acylhydrolase
-
-
-
-
phospholipase A1/2
247
-
phospholipase A1beta
247
-
phospholipase A1gamma
247
-
PLA 1
247
-
PLA1
247
-
PLA1/2
247
-
PLA1A
PLA1gamma
247
-
PLA1S
247
-
PLRP2
additional information
247
the isozymes belong to the pancreatic lipase gene family or to the phospholipase family
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
phosphatidylcholine + H2O = 2-acylglycerophosphocholine + a carboxylate
show the reaction diagram
structure-function relationship
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of carboxylic ester
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
phosphatidylcholine 1-acylhydrolase
This enzyme has a much broader specificity than EC 3.1.1.4 phospholipase A2. Requires Ca2+.
CAS REGISTRY NUMBER
COMMENTARY hide
9043-29-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
phosphatidic acid + H2O
2-acyl-lysophosphatidic acid + a carboxylate
show the reaction diagram
-
-
?
dipalmitoylphosphatidylcholine + H2O
palmitic acid + 1-lyso-2-palmitoylphosphatidylcholine
show the reaction diagram
-
-
-
-
?
high-density lipoprotein + H2O
fatty acid + ?
show the reaction diagram
-
-
-
-
?
phosphatidic acid + H2O
2-acyl-lysophosphatidic acid + a carboxylate
show the reaction diagram
phosphatidic acid + H2O
?
show the reaction diagram
-
phosphatidic acid-specific isozymes mPA-PLA1alpha and mPA-PLA1beta
-
-
?
phosphatidylcholine + H2O
2-acylglycerophosphocholine + a carboxylate
show the reaction diagram
phosphatidylcholine + H2O
2-acylglycerophosphocholine + a fatty acid anion
show the reaction diagram
-
-
-
-
?
phosphatidylcholine + H2O
lysophosphatidylcholine + fatty acid
show the reaction diagram
-
-
-
-
?
phosphatidylethanolamine + H2O
2-acylglycerophosphoethanolamine + a carboxylate
show the reaction diagram
phosphatidylethanolamine + H2O
?
show the reaction diagram
phosphatidylethanolamine + H2O
lysophosphatidylethanolamine + a fatty acid anion
show the reaction diagram
-
-
-
-
?
phosphatidylserine + H2O
2-acylglycerophosphoserine + a carboxylate
show the reaction diagram
-
low activity
-
?
phosphatidylserine + H2O
?
show the reaction diagram
phosphatidylserine + H2O
lysophosphatidylserine + fatty acid
show the reaction diagram
-
-
-
-
?
sphingomyelin + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
phosphatidic acid + H2O
2-acyl-lysophosphatidic acid + a carboxylate
show the reaction diagram
Q6XZB0
-
-
?
phosphatidic acid + H2O
2-acyl-lysophosphatidic acid + a carboxylate
show the reaction diagram
phosphatidylcholine + H2O
2-acylglycerophosphocholine + a carboxylate
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
H-Rev107 functions as a cytosolic Ca2+-independent PLA1/2
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Sodium vanadate
0.01 mM, 50% inhibition
Cationic amphiphilic drugs
-
-
-
GSK264220A
-
-
Insulin
-
significantly reduces endothelial lipase mRNA and protein expression in a dose dependent manner
-
iodoacetic acid
-
-
tetrahydrolipstatin
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dithiothreitol
-
-
tumor necrosis factor-alpha
-
stimulates secretion of endothelial lipase, the stimulation can be inhibited by adiponectin
-
additional information
-
endothelial lipase is upregulated by inflammation
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.1
high-density lipoprotein
-
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
high mRNA levels
Manually annotated by BRENDA team
-
isozyme endothelial lipase
Manually annotated by BRENDA team
high mRNA levels
Manually annotated by BRENDA team
-
northern analysis
Manually annotated by BRENDA team
high mRNA levels
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
northern analysis
Manually annotated by BRENDA team
-
low expression
Manually annotated by BRENDA team
low mRNA levels
Manually annotated by BRENDA team
-
high expression
Manually annotated by BRENDA team
additional information
-
not expressed in SIMA cell, SH-SY5Y cell, IMR-5 cell, KELLY cell, NB-1 cell, NB-2 cell, NB-3 cell, Nb-4 cell, ERMS-7 cell, ERMS-8 cell, ERMS-10 cell, ARMS-7 cell, ARMS-8 cell, ARMS-9 cell, ARMS-10 cell, adrenal gland, prostate gland, uterus, salivary gland, skeletal muscle, thymus, adult brain, cerebellum, fetal liver kidney, bone marrow, liver, lung, heart, and placenta
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
cis-Golgi and endoplasmic reticulum-Golgi intermediate compartment (ERGIC). Endogenous intracellular PLA1gamma does not appear to be localized to the ERGIC at 37°C. At 15°C, intracellular PLA1gamma shows marked colocalization with ERGIC-53
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
Sequence
LIPI_HUMAN
460
0
52922
Swiss-Prot
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
52991
x * 52991, deduced from nucleotide sequence
55000
x * 55000, SDS-PAGE, immunoblot
90000
-
x * 90000, SDS-PAGE, immunoblot
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 52991, deduced from nucleotide sequence; x * 55000, SDS-PAGE, immunoblot
?
-
x * 90000, SDS-PAGE, immunoblot
additional information
-
isozyme tertiary structure analysis, amino acid sequence comparison
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
N336Q
-
the mutant shows a significant phospholipase A1 activity on mixed phospholipid-bile salt micelles
S149A
-
catalytically inactive
S537A
-
site-directed mutagenesis, inactive mutant
additional information
PURIFICATION/commentary
ORGANISM
UNIPROT
LITERATURE
heparin-Sepharose column chromatography and Superdex 200 gel filtration
-
native enzyme 1500fold from thrombin-activated platelets by anion exchange, hydrophobic interaction, and affinity chromatography
-
partial
-
CLONED/commentary
ORGANISM
UNIPROT
LITERATURE
cDNA encoding C-terminally FLAG-tagged H-Rev107. Subcloned into the HindIII and EcoRI sites of pcDNA3.1 (+). Expression vector harboring H-Rev107 cDNA introduced into COS-7 cells
-
cloning of cDNA, transient expression in 293 T cells
-
DNA and amino acid sequence analysis of isozymes, isozyme genetic structures, location of isozyme PS-PLA1 or PLA1A encoding gene on chromosome 3q13.2-q13.3, phylogenetic analysis of isozymes, overview
-
expressed in CHO cells
-
expressed in HEK-293 cells
-
expression HeLa, CHO-K1 and Sf9 cells
expression in Sf9 insect cells
expression of FLAG-tagged wild-type and mutant enzymes in HeLa cells and in siRNA-treated knockout mutant HeLa cells. Upon overexpression of wild-type enzyme, the tubular structures become fragmented, expression of the mutant S537A has no effect
-
polypeptide (amino acid number 368-486 of intracellular PLA1gamma) expressed as a glutathione S-transferase fusion protein in Escherichia coli. Purified protein used to immunize rats. The cDNA encoding intracellular PLA1gamma cloned into pEGFP-C3 vector and stably expressed in CHO cells
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression of intracellular PLA1gamma is suppressed by transfecting cells with siRNA against intracellular PLA1gamma. Expression of intracellular PLA1gamma protein is decreased to less than 10% that of control siRNA-treated cells
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Löffler, B.M.; Kunze, H.
Fractionation, biochemical characterization and lysosomal phospholipases of human liver
FEBS Lett.
216
51-56
1987
Homo sapiens
Manually annotated by BRENDA team
Bellini, F.; Bruni, A.
Role of a serum phospholipase A1 in the phosphati dylserine-induced T cell inhibition
FEBS Lett.
316
1-4
1993
Bos taurus, Homo sapiens
Manually annotated by BRENDA team
Nakajima, K.; Sonoda, H.; Mizoguchi, T.; Aoki, J.; Arai, H.; Nagahama, M.; Tagaya, M.; Tani, K.
A novel phospholipase A1 with sequence homology to a mammalian Sec23p-interacting protein, p125
J. Biol. Chem.
277
11329-11335
2002
Homo sapiens
Manually annotated by BRENDA team
Sonoda, H.; Aoki, J.; Hiramatsu, T.; Ishida, M.; Bandoh, K.; Nagai, Y.; Taguchi, R.; Inoue, K.; Arai, H.
A novel phosphatidic acid-selective phospholipase A1 that produces lysophosphatidic acid
J. Biol. Chem.
277
34254-34263
2002
Homo sapiens, Homo sapiens (Q8WWY8)
Manually annotated by BRENDA team
Hiramatsu, T.; Sonoda, H.; Takanezawa, Y.; Morikawa, R.; Ishida, M.; Kasahara, K.; Sanai, Y.; Taguchi, R.; Aoki, J.; Arai, H.
Biochemical and molecular characterization of two phosphatidic acid-selective phospholipase A1s, mPA-PLA1alpha and mPA-PLA1beta
J. Biol. Chem.
278
49438-49447
2003
Homo sapiens, Homo sapiens (Q6XZB0)
Manually annotated by BRENDA team
Chen, S.; Subbaiah, P.V.
Phospholipid and fatty acid specificity of endothelial lipase: Potential role of the enzyme in the delivery of docosahexaenoic acid (DHA) to tissues
Biochim. Biophys. Acta
1771
1319-1328
2007
Homo sapiens
Manually annotated by BRENDA team
Aoki, J.; Inoue, A.; Makide, K.; Saiki, N.; Arai, H.
Structure and function of extracellular phospholipase A1 belonging to the pancreatic lipase gene family
Biochimie
89
197-204
2007
Bombyx mori, Cavia porcellus, Dolichovespula maculata, Drosophila melanogaster, Homo sapiens, Mus musculus, Rattus norvegicus
Manually annotated by BRENDA team
Qiu, G.; Hill, J.S.
Endothelial lipase promotes apolipoprotein AI-mediated cholesterol efflux in THP-1 macrophages
Arterioscler. Thromb. Vasc. Biol.
29
84-91
2009
Homo sapiens
Manually annotated by BRENDA team
Shiu, S.W.; Tan, K.C.; Huang, Y.; Wong, Y.
Type 2 diabetes mellitus and endothelial lipase
Atherosclerosis
198
441-447
2008
Homo sapiens
Manually annotated by BRENDA team
Eydoux, C.; Spinelli, S.; Davis, T.L.; Walker, J.R.; Seitova, A.; Dhe-Paganon, S.; De Caro, A.; Cambillau, C.; Carriere, F.
Structure of human pancreatic lipase-related protein 2 with the lid in an open conformation
Biochemistry
47
9553-9564
2008
Homo sapiens
Manually annotated by BRENDA team
Badellino, K.O.; Wolfe, M.L.; Reilly, M.P.; Rader, D.J.
Endothelial lipase is increased in vivo by inflammation in humans
Circulation
117
678-685
2008
Homo sapiens
Manually annotated by BRENDA team
Keller, P.M.; Rust, T.; Murphy, D.J.; Matico, R.; Trill, J.J.; Krawiec, J.A.; Jurewicz, A.; Jaye, M.; Harpel, M.; Thrall, S.; Schwartz, B.
A high-throughput screen for endothelial lipase using HDL as substrate
J. Biomol. Screen.
13
468-475
2008
Homo sapiens
Manually annotated by BRENDA team
Foell, J.L.; Hesse, M.; Volkmer, I.; Schmiedel, B.J.; Neumann, I.; Staege, M.S.
Membrane-associated phospholipase A1 beta (LIPI) is an ewing tumour-associated cancer/testis antigen
Pediatr. Blood Cancer
51
228-234
2008
Homo sapiens
Manually annotated by BRENDA team
Morikawa, R.K.; Aoki, J.; Kano, F.; Murata, M.; Yamamoto, A.; Tsujimoto, M.; Arai, H.
Intracellular phospholipase A1gamma (iPLA1gamma) is a novel factor involved in coat protein complex I- and Rab6-independent retrograde transport between the endoplasmic reticulum and the Golgi complex
J. Biol. Chem.
284
26620-26630
2009
Homo sapiens
Manually annotated by BRENDA team
Uyama, T.; Morishita, J.; Jin, X.H.; Okamoto, Y.; Tsuboi, K.; Ueda, N.
The tumor suppressor gene H-Rev107 functions as a novel Ca2+-independent cytosolic phospholipase A1/2 of the thiol hydrolase type
J. Lipid Res.
50
685-693
2009
Homo sapiens, Mus musculus, Rattus norvegicus (P53817)
Manually annotated by BRENDA team
Bolen, A.L.; Naren, A.P.; Yarlagadda, S.; Beranova-Giorgianni, S.; Chen, L.; Norman, D.; Baker, D.L.; Rowland, M.M.; Best, M.D.; Sano, T.; Tsukahara, T.; Liliom, K.; Igarashi, Y.; Tigyi, G.
The phospholipase A1 activity of lysophospholipase A-I links platelet activation to LPA production during blood coagulation
J. Lipid Res.
52
958-970
2011
Homo sapiens
Manually annotated by BRENDA team
Baba, T.; Kashiwagi, Y.; Arimitsu, N.; Kogure, T.; Edo, A.; Maruyama, T.; Nakao, K.; Nakanishi, H.; Kinoshita, M.; Frohman, M.A.; Yamamoto, A.; Tani, K.
Phosphatidic acid (PA)-preferring phospholipase A1 regulates mitochondrial dynamics
J. Biol. Chem.
289
11497-11511
2014
Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Iida, Y.; Sunami, E.; Yamashita, H.; Hiyoshi, M.; Ishihara, S.; Yamaguchi, H.; Inoue, A.; Makide, K.; Tsuno, N.H.; Aoki, J.; Kitayama, J.; Watanabe, T.
Phosphatidylserine-specific phospholipase A1 (PS-PLA1) expression in colorectal cancer correlates with tumor invasion and hematogenous metastasis
Anticancer Res.
35
1459-1464
2015
Homo sapiens (Q53H76)
Manually annotated by BRENDA team
Dard, R.; Meyniel, C.; Touitou, V.; Stevanin, G.; Lamari, F.; Durr, A.; Ewenczyk, C.; Mochel, F.
Mutations in DDHD1, encoding a phospholipase A1, is a novel cause of retinopathy and neurodegeneration with brain iron accumulation
Eur. J. Med. Genet.
60
639-642
2017
Homo sapiens (Q8NEL9)
Manually annotated by BRENDA team
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