Any feedback?
Information on EC 3.1.1.31 - 6-phosphogluconolactonase and Organism(s) Homo sapiens and UniProt Accession O95336
for references in articles please use BRENDA:EC3.1.1.31Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
3.1.1.31 6-phosphogluconolactonaseSpecify your search results
The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
6-phosphogluconolactonase, 6pgl, phosphogluconolactonase, glucose-6-phosphate dehydrogenase-6-phosphogluconolactonase, g6pd-6pgl, 6-pgl, 6-phosphogluconolactonase 3, 6-phosphoglucono-gamma-lactonase, xoo2316, og1rf_11582, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
6-PGL
-
-
-
-
6-phospho-D-glucose-delta-lactone hydrolase
-
-
-
-
6-phosphoglucono-gamma-lactonase
-
-
-
-
6PGL
lactonase, phosphoglucono-
-
-
-
-
PGL
-
-
-
-
phosphogluconolactonase
-
-
-
-
6PGL
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
6-phospho-D-glucono-1,5-lactone + H2O = 6-phospho-D-gluconate
first order reaction
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of carboxylic ester
-
-
-
-
PATHWAY SOURCE
PATHWAYS
BRENDA
KEGG
-
-, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
6-phospho-D-glucono-1,5-lactone lactonohydrolase
-
CAS REGISTRY NUMBER
COMMENTARY
37278-45-8
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
6-phospho-D-glucono-1,4-lactone + H2O
6-phospho-D-gluconate
the gamma-lactone is more stable than the natural delta-lactone substrate. It contains a five-membered heterocyclic ring, whereas the delta-lactone contains a six-membered heterocyclic ring
-
-
?
6-phospho-D-glucono-1,5-lactone + H2O
6-phospho-D-gluconate
-
6-phosphogluconolactone
-
?
6-phospho-D-glucono-1,5-lactone + H2O
6-phospho-D-gluconate
-
6-phosphogluconolactone
-
?
6-phospho-D-glucono-1,5-lactone + H2O
6-phospho-D-gluconate
-
enzyme hydrolyzes both, the delta- and - gamma forms of 6-phosphogluconolactone
-
?
6-phospho-D-glucono-1,5-lactone + H2O
6-phospho-D-gluconate
-
6-phospho-D-gluconate
-
?
6-phospho-D-glucono-1,5-lactone + H2O
6-phospho-D-gluconate
-
second step in pentose phosphate pathway
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
6-phospho-D-glucono-1,5-lactone + H2O
6-phospho-D-gluconate
-
second step in pentose phosphate pathway
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
1065
recombinant h6PGL, substrate is 6-phospho-D-glucono-1,5-lactone, pH 7.0, 25°C
additional information
additional information
-
enzyme activity in lymphocytes, monocytes, granulocytes and platelets is 10 times as high as that in erythrocytes, activity is independent of red cell age
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
25
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). 6PGL_HUMAN
258
0
27547
Swiss-Prot
other Location (Reliability: 4)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
22°C, enzyme activity in erythrocytes is stable for 6 days stored in citrate dextrose, heparin and EDTA as anticoagulants
-
4°C, enzyme activity in erythrocytes is stable for 20 days stored in citrate dextrose, heparin and EDTA as anticoagulants
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene h6PGL, overexpression as N-terminally His-tagged enzyme in Escherichia coli strain BL21 using vector pRAREII
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Bauer, H.P.; Srihari, T.; Jochims, J.C.; Hofer, H.W.
6-Phosphogluconolactonase. Purification, properties and activities in various tissues
Eur. J. Biochem.
133
163-168
1983
Bos taurus, Homo sapiens, Rattus norvegicus
Beutler, E.; Kuhl, W.; Gelbart, T.
Blood cell phosphogluconolactonase: assay and properties
Br. J. Haematol.
62
577-586
1986
Homo sapiens
Rakitzis, E.T.; Papandreou, P.
Kinetic analysis of 6-phosphogluconolactone hydrolysis in hemolysates
Biochem. Mol. Biol. Int.
37
747-755
1995
Homo sapiens
Collard, F.; Collet, J.F.; Gerin, I.; Veiga-da-Cunha, M.; van Schaftingen, E.
Identification of the cDNA encoding human 6-phosphogluconolactonase, the enzyme catalyzing the second step of the pentose phosphate pathway(1)
FEBS Lett.
459
223-226
1999
Homo sapiens
Clarke, J.L.; Scopes, D.A.; Sodeinde, O.; Mason, P.J.
Glucose-6-phosphate dehydrogenase-6-phosphogluconolactonase. A novel bifunctional enzyme in malaria parasites
Eur. J. Biochem.
268
2013-2019
2001
Homo sapiens, Plasmodium berghei
Miclet, E.; Stoven, V.; Michels, P.A.M.; Opperdoes, F.R.; Lallemand, J.Y.; Duffieux, F.
NMR spectroscopic analysis of the first two steps of the pentose-phosphate pathway elucidates the role of 6-phosphogluconolactonase
J. Biol. Chem.
276
34840-34846
2001
Homo sapiens (O95336), Homo sapiens, Plasmodium falciparum (Q27741), Plasmodium falciparum, Trypanosoma brucei (Q9GRG6), Trypanosoma brucei
Jortzik, E.; Mailu, B.M.; Preuss, J.; Fischer, M.; Bode, L.; Rahlfs, S.; Becker, K.
Glucose-6-phosphate dehydrogenase-6-phosphogluconolactonase: a unique bifunctional enzyme from Plasmodium falciparum
Biochem. J.
436
641-650
2011
Plasmodium falciparum, Homo sapiens (O95336), Homo sapiens
EXTERNAL LINKS (specific for EC-Number 3.1.1.31)
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
