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Information on EC 3.1.1.29 - aminoacyl-tRNA hydrolase

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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.29 aminoacyl-tRNA hydrolase
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This record set is specific for:
UNIPROT: Q9H8Y5 not found.
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Word Map
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The enzyme appears in viruses and cellular organisms
Synonyms
peptidyl-trna hydrolase, ankzf1, ptrhd1, spovc, mspth, bacterial peptidyl-trna hydrolase, peptidyl-trna hydrolase 2, yhr189w, abpth, cdc48 adaptor, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
peptidyl-tRNA hydrolase
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aminoacyl-transfer ribonucleate hydrolase
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hydrolase, aminoacyl-transfer ribonucleate
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N-substituted aminoacyl transfer RNA hydrolase
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peptidyl-tRNA hydrolase
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PTH
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of carboxylic ester
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
aminoacyl-tRNA aminoacylhydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
9054-98-2
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Flag-tagged CRPNSKn-tRNA + H2O
Flag-tagged CRPNSKn + tRNA
show the reaction diagram
purified recombinant Ankzf1 catalyzes deacylation
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?
peptidyl-tRNA + H2O
peptide + tRNA
show the reaction diagram
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?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
peptidyl-tRNA + H2O
peptide + tRNA
show the reaction diagram
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
Ankzf1 is Saccharomyces cerevisiae Vms1 homologue
physiological function
Ankzf1 is a peptidyl-tRNA hydrolase
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
ANKZ1_HUMAN
726
0
80927
Swiss-Prot
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Q246L
site-directed mutagenesis, inactive active site mutant
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene ANKZF1, transient recombinant expression of His-tagged wild-type and mutant enzymes in HEK-293 cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Verma, R.; Reichermeier, K.M.; Burroughs, A.M.; Oania, R.S.; Reitsma, J.M.; Aravind, L.; Deshaies, R.J.
Vms1 and ANKZF1 peptidyl-tRNA hydrolases release nascent chains from stalled ribosomes
Nature
557
446-451
2018
Homo sapiens (Q9H8Y5), Saccharomyces cerevisiae (Q04311), Saccharomyces cerevisiae, Saccharomyces cerevisiae ATCC 204508 (Q04311)
Manually annotated by BRENDA team