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Information on EC 3.1.1.22 - hydroxybutyrate-dimer hydrolase
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3.1.1.22 hydroxybutyrate-dimer hydrolaseSpecify your search results
Word Map
The enzyme appears in viruses and cellular organisms
Synonyms
phaz1, phaz2, phazc, intracellular phb depolymerase, 3hboh, 3-hydroxybutyrate-oligomer hydrolase, d(-)-3-hydroxybutyrate oligomer hydrolase, 3hb-oligomer hydrolase, d-(-)-3-hydroxybutyrate oligomer hydrolase, 3-hydroxybutyrate oligomer hydrolase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
3-HB-oligomer hydrolase
3-hydroxybutyrate oligomer hydrolase
3HB oligomer hydrolase
3HB-oligomer hydrolase
-
-
-
-
3HBOH
D-(-)-3-hydroxybutyrate oligomer hydrolase
D-(-)-3-hydroxybutyrate-dimer hydrolase
-
-
-
-
hydrolase, hydroxybutyrate dimer
-
-
-
-
PhaZ2
PhaZc
additional information
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
(R)-3-((R)-3-hydroxybutanoyloxy)butanoate + H2O = 2 (R)-3-hydroxybutanoate
-
-
-
-
(R)-3-((R)-3-hydroxybutanoyloxy)butanoate + H2O = 2 (R)-3-hydroxybutanoate
Ser359 is invlved in the catalytic mechanism, active site residue
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of carboxylic ester
hydrolysis of carboxylic ester
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
KEGG
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SYSTEMATIC NAME
IUBMB Comments
(R)-3-((R)-3-hydroxybutanoyloxy)butanoate hydroxybutanoylhydrolase
-
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CAS REGISTRY NUMBER
COMMENTARY
37278-37-8
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(R)-3-((S)-3-hydroxybutanoyloxy)butanoate + H2O
(R)-3-hydroxybutanoate + (S)-3-hydroxybutanoate
(S)-3-((R)-3-hydroxybutanoyloxy)butanoate + H2O
(R)-3-hydroxybutanoate + (S)-3-hydroxybutanoate
-
i.e. L-(+)-3-[D-(-)-hydroxybutuyryloxy]butyric acid
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
75% of the activity with 4-nitrophenyl butyrate
-
?
4-nitrophenyl butyrate + H2O
4-nitrophenol + butyrate
-
best 4-nitrophenyl ester substrate
-
?
4-nitrophenyl hexanoate + H2O
4-nitrophenol + hexanoate
-
43% of the activity with 4-nitrophenyl butyrate
-
?
4-nitrophenyl propionate + H2O
4-nitrophenol + propionate
-
81% of the activity with 4-nitrophenyl butyrate
-
?
(R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O
(R)-3-hydroxybutanoate
-
-
-
-
?
(R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O
(R)-3-hydroxybutanoate
-
involved in the degradation of poly-3-hydroxybutyrate (PHB)
-
-
?
(R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O
(R)-3-hydroxybutanoate
-
-
-
-
?
(R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O
(R)-3-hydroxybutanoate
-
involved in the degradation of poly-3-hydroxybutyrate (PHB)
-
-
?
(R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O
(R)-3-hydroxybutanoate
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
-
-
-
?
(R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O
(R)-3-hydroxybutanoate
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
involved in the degradation of poly-3-hydroxybutyrate (PHB)
-
-
?
(R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O
(R)-3-hydroxybutanoate
-
-
-
-
?
(R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O
(R)-3-hydroxybutanoate
-
involved in the degradation of poly-3-hydroxybutyrate (PHB)
-
-
?
(R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O
(R)-3-hydroxybutanoate
-
-
-
-
?
(R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O
(R)-3-hydroxybutanoate
-
involved in the degradation of poly-3-hydroxybutyrate (PHB)
-
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)11butanoate + H2O
(R)-3-hydroxybutanoate
-
exo-type hydrolysis from the carboxyl terminus
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)11butanoate + H2O
(R)-3-hydroxybutanoate
-
-
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)11butanoate + H2O
(R)-3-hydroxybutanoate
-
-
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)11butanoate + H2O
(R)-3-hydroxybutanoate
-
-
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)2butanoate + H2O
(R)-3-hydroxybutanoate
-
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)2butanoate + H2O
(R)-3-hydroxybutanoate
-
exo-type hydrolysis from the carboxyl terminus
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)2butanoate + H2O
(R)-3-hydroxybutanoate
-
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)2butanoate + H2O
(R)-3-hydroxybutanoate
-
-
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)2butanoate + H2O
(R)-3-hydroxybutanoate
-
-
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)2butanoate + H2O
(R)-3-hydroxybutanoate
-
-
-
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)2butanoate + H2O
(R)-3-hydroxybutanoate
-
-
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)2butanoate + H2O
(R)-3-hydroxybutanoate
-
-
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)3butanoate
?
-
degradation of poly-(3-hydroxybutyrate)
-
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)3butanoate
?
-
degradation of poly-(3-hydroxybutyrate)
-
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)3butanoate
?
-
degradation of poly-(3-hydroxybutyrate)
-
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)3butanoate
?
-
degradation of poly-(3-hydroxybutyrate)
-
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)3butanoate + H2O
(R)-3-hydroxybutanoate
-
exo-type hydrolysis from the carboxyl terminus
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)3butanoate + H2O
(R)-3-hydroxybutanoate
-
-
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)3butanoate + H2O
(R)-3-hydroxybutanoate
-
-
-
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)3butanoate + H2O
(R)-3-hydroxybutanoate
-
-
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)3butanoate + H2O
(R)-3-hydroxybutanoate
-
attack from th free hydroxyl terminus, releasing monomer units one at a time
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)4butanoate + H2O
(R)-3-hydroxybutanoate
-
exo-type hydrolysis from the carboxyl terminus
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)4butanoate + H2O
(R)-3-hydroxybutanoate
-
-
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)4butanoate + H2O
(R)-3-hydroxybutanoate
-
-
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)4butanoate + H2O
(R)-3-hydroxybutanoate
-
-
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)7butanoate + H2O
(R)-3-hydroxybutanoate
-
exo-type hydrolysis from the carboxyl terminus
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)7butanoate + H2O
(R)-3-hydroxybutanoate
-
-
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)7butanoate + H2O
(R)-3-hydroxybutanoate
-
-
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)7butanoate + H2O
(R)-3-hydroxybutanoate
-
-
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)butanoate + H2O
(R)-3-hydroxybutanoate
-
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)butanoate + H2O
(R)-3-hydroxybutanoate
-
i.e. D-(-)-3-[hydroxybutuyryloxy]butyric acid
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)butanoate + H2O
(R)-3-hydroxybutanoate
-
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)butanoate + H2O
(R)-3-hydroxybutanoate
-
preferred substrate
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)butanoate + H2O
(R)-3-hydroxybutanoate
-
-
-
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)butanoate + H2O
(R)-3-hydroxybutanoate
-
i.e. D-(-)-3-[hydroxybutuyryloxy]butyric acid
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)butanoate + H2O
(R)-3-hydroxybutanoate
-
i.e. D-(-)-3-[hydroxybutuyryloxy]butyric acid
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)butanoate + H2O
(R)-3-hydroxybutanoate
-
i.e. D-(-)-3-[hydroxybutuyryloxy]butyric acid
-
?
(R)-3-((S)-3-hydroxybutanoyloxy)butanoate + H2O
(R)-3-hydroxybutanoate + (S)-3-hydroxybutanoate
-
i.e. D-(-)-3-[L-(+)-hydroxybutuyryloxy]butyric acid
-
?
(R)-3-((S)-3-hydroxybutanoyloxy)butanoate + H2O
(R)-3-hydroxybutanoate + (S)-3-hydroxybutanoate
-
i.e. D-(-)-3-[L-(+)-hydroxybutuyryloxy]butyric acid
-
?
3-(3-hydroxybutanoyloxy)butanoate + H2O
3-hydroxybutanoate
-
-
-
?
3-(3-hydroxybutanoyloxy)butanoate + H2O
3-hydroxybutanoate
-
-
-
?
3-(3-hydroxypentanoyloxy)pentanoate + H2O
3-hydroxypentanoate
-
-
-
?
3-(3-hydroxypentanoyloxy)pentanoate + H2O
3-hydroxypentanoate
-
-
-
?
4-nitrophenyl butyrate + H2O
4-nitrophenol + butanoate
-
-
-
?
4-nitrophenyl butyrate + H2O
4-nitrophenol + butanoate
-
-
-
?
4-nitrophenyl pentanoate + H2O
4-nitrophenol + pentanoate
-
-
-
?
4-nitrophenyl pentanoate + H2O
4-nitrophenol + pentanoate
-
60% of the activity with 4-nitrophenyl butyrate
-
?
4-nitrophenyl pentanoate + H2O
4-nitrophenol + pentanoate
-
-
-
?
additional information
?
-
-
hydrolyzes exclusively 3-hydroxybutyrate-dimers and trimers, no hydrolysis of longer linear or cyclic oligomers
-
-
?
additional information
?
-
-
hydrolyzes 3-hydroxybutyrate-dimers, trimers, tetramers, pentamers and cyclic trimers, pentamers and hexamers at a similar efficiency
-
-
?
additional information
?
-
enzyme PhaZ1 converts the polymer into oligomers and monomers of hydroxybutyrate by the end of the degradation process
-
-
?
additional information
?
-
enzyme PhaZ1 converts the polymer into oligomers and monomers of hydroxybutyrate by the end of the degradation process
-
-
?
additional information
?
-
-
enzyme PhaZ1 converts the polymer into oligomers and monomers of hydroxybutyrate by the end of the degradation process
-
-
?
additional information
?
-
the enzyme shows no activity with 3HB oligomers or poly(hydroxybutyrate), PHB, granules
-
-
?
additional information
?
-
-
the enzyme shows no activity with 3HB oligomers or poly(hydroxybutyrate), PHB, granules
-
-
?
additional information
?
-
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
hydrolyzes 3-hydroxybutyrate-dimers, trimers, tetramers, pentamers and cyclic trimers, pentamers and hexamers at a similar efficiency
-
-
?
additional information
?
-
pHB and PHV granules are not depolymerized by the enzyme
-
-
?
additional information
?
-
-
pHB and PHV granules are not depolymerized by the enzyme
-
-
?
additional information
?
-
pHB and PHV granules are not depolymerized by the enzyme
-
-
?
additional information
?
-
-
hydrolyzes 3-hydroxybutyrate-dimers, trimers, tetramers, pentamers and cyclic trimers, pentamers and hexamers at a similar efficiency
-
-
?
additional information
?
-
-
hydrolyzes 3-hydroxybutyrate-dimers, trimers, tetramers, pentamers and cyclic trimers, pentamers and hexamers at a similar efficiency
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O
(R)-3-hydroxybutanoate
-
involved in the degradation of poly-3-hydroxybutyrate (PHB)
-
-
?
(R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O
(R)-3-hydroxybutanoate
-
involved in the degradation of poly-3-hydroxybutyrate (PHB)
-
-
?
(R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O
(R)-3-hydroxybutanoate
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
involved in the degradation of poly-3-hydroxybutyrate (PHB)
-
-
?
(R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O
(R)-3-hydroxybutanoate
-
involved in the degradation of poly-3-hydroxybutyrate (PHB)
-
-
?
(R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O
(R)-3-hydroxybutanoate
-
involved in the degradation of poly-3-hydroxybutyrate (PHB)
-
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)3butanoate
?
-
degradation of poly-(3-hydroxybutyrate)
-
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)3butanoate
?
-
degradation of poly-(3-hydroxybutyrate)
-
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)3butanoate
?
-
degradation of poly-(3-hydroxybutyrate)
-
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)3butanoate
?
-
degradation of poly-(3-hydroxybutyrate)
-
-
?
additional information
?
-
-
hydrolyzes exclusively 3-hydroxybutyrate-dimers and trimers, no hydrolysis of longer linear or cyclic oligomers
-
-
?
additional information
?
-
-
hydrolyzes 3-hydroxybutyrate-dimers, trimers, tetramers, pentamers and cyclic trimers, pentamers and hexamers at a similar efficiency
-
-
?
additional information
?
-
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
hydrolyzes 3-hydroxybutyrate-dimers, trimers, tetramers, pentamers and cyclic trimers, pentamers and hexamers at a similar efficiency
-
-
?
additional information
?
-
-
hydrolyzes 3-hydroxybutyrate-dimers, trimers, tetramers, pentamers and cyclic trimers, pentamers and hexamers at a similar efficiency
-
-
?
additional information
?
-
-
hydrolyzes 3-hydroxybutyrate-dimers, trimers, tetramers, pentamers and cyclic trimers, pentamers and hexamers at a similar efficiency
-
-
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
phenylmethylsulfonyl fluoride
27.4% residual activity at 1 mM
additional information
-
not inhibited by 1,4-dimercapto-2,3-butanediol; not inhibited by (p-tert-octylphenoxy)polyethoxyethanol
-
diisopropylfluorophosphate
completely inhibited by 1 mM diisopropylfluorophosphate
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.48
with 3-(3-hydroxypentanoyloxy)pentanoate as substrate, at pH 8.0 and 30°C
0.67
with 3-(3-hydroxybutanoyloxy)butanoate as substrate, at pH 8.0 and 30°C
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
no activity in Cupriavidus necator
i.e. Ralstonia eutropha, in strain H16 polyhydroxybutyrate granules
-
-
brenda
formerly Alcaligenes eutrophus, strain H16, ATCC 17699
SwissProt
brenda
i.e. Alcaligenes eutrophus or Ralstonia eutropha
UniProt
brenda
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE