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Information on EC 3.1.1.20 - tannase
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3.1.1.20 tannaseIUBMB Comments
Also hydrolyses ester links in other tannins.
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Word Map
- 3.1.1.20
-
gallic
-
tannic
- aspergillus
- niger
- gallate
- plantarum
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submerged
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solid-state
- food industry
- catechin
- pectinase
- biotechnology
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tannery
- gallotannins
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galloylated
-
hydrolysable
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paecilomyces
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feruloyl
-
tannin-rich
- 1-propanol
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depside
- emblica
- pentosus
- synthesis
- variotii
- degradation
- industry
- medicine
- agriculture
- brewing
- nutrition
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
tannase, fungal tannase, tannin acyl hydrolase, tanlpl, tansg1, aotanb, atan1, lp-tan, depsidase, tan410, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
An04g04430
AoTanA
AotanB
ATAN1
depsidase
fungal tannase
GALLO_1609
TAH
TAH I
TAH II
tan A
Tan410
tan7
TanA
TanB
tanBLP
tannase
tannin acyl hydrolase
tannin acyl-hydrolase
tannin acylhydrolase
tannin-acyl-hydrolase
TanSg1
yeast tannase
tannase
Hyalopus sp. DSF3
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tannin acyl hydrolase
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tannin acyl hydrolase
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belongs to the class of serine hydrolases
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
digallate + H2O = 2 gallate
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of carboxylic ester
hydrolysis of carboxylic ester
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-
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hydrolysis of carboxylic ester
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the enzyme also shows beta-glucosidase activity
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SYSTEMATIC NAME
IUBMB Comments
tannin acylhydrolase
Also hydrolyses ester links in other tannins.
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CAS REGISTRY NUMBER
COMMENTARY
9025-71-2
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(-)-gallocatechin gallate + H2O
(-)-gallocatechin + gallic acid
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-
-
?
1-O-p-hydroxybenzoyl-beta-D-glucose + H2O
4-hydroxybenzoate + beta-D-glucose
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-
-
-
?
1-O-syringoyl-beta-D-glucose + H2O
4-hydroxy-3,5-dimethoxy benzoic acid + beta-D-glucose
-
-
-
-
?
1-O-vanilloyl-beta-D-glucose + H2O
4-hydroxy-3-methoxy benzoic acid + beta-D-glucose
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-
-
-
?
1-O-veratroyl-beta-D-glucose + H2O
3,4-dimethoxybenzoic acid + beta-D-glucose
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-
-
-
?
1-propanol + gallic acid
propyl gallate + H2O
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the reaction takes place in organic solvents, best in benzene, effect of water content, overview
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r
cellobiose + H2O
2 D-glucose
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beta-glucosidase activity of the enzyme, only observed in absence of tannic acid
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?
chlorogenic acid + H2O
trans-caffeate + quinate
complete hydrolysis within 40 min
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-
?
ethyl ferulate + H2O
ethanol + ferulate
complete hydrolysis within 40 min
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-
?
ethyl protocatechuate + H2O
ethanol + protocatechuate
very low activity
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-
?
gallate + 1-butanol
1-butyl gallate + H2O
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85% of the yield with 1-propanol
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r
gallate + 1-hexanol
1-hexyl gallate + H2O
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2% of the yield with 1-propanol
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r
gallate + 1-pentanol
1-pentyl gallate + H2O
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77% of the yield with 1-propanol
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r
gallate + ethanol
ethyl gallate + H2O
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5% of the yield with 1-propanol
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r
gallate + methanol
methyl gallate + H2O
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2% of the yield with 1-propanol
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r
gallic acid ethyl ester + H2O
gallic acid + ethanol
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-
-
?
gallic acid propyl ester + H2O
gallic acid + propanol
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-
-
?
isoamyl gallate + H2O
gallic acid + isoamyl alcohol
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35% activity compared to methyl gallate
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-
?
monogalloyl glucose + H2O
gallic acid + D-glucose
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37% activity compared to methyl gallate
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?
protocatechuic acid ethyl ester + H2O
protocatechuate + ethanol
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?
rosmarinic acid + H2O
(2E)-3,4-dihydroxycinnamate + (2R)-3-(3,4-dihydroxyphenyl)-2-hydroxypropanoate
88% hydrolysis within 40 min
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?