Information on EC 3.1.1.20 - tannase

-

89% activity compared to methyl gallate

-

?

-

-

1-O-galloyl-beta-D-glucose + H2O

gallic acid + beta-D-glucose

-

-

-

-

?

butyl 4-hydroxybenzoate + H2O

butanol + 4-hydroxybenzoate

-

-

?

-

-

?

catechin gallate + H2O

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

catechin gallate + H2O

gallic acid + catechin

-

-

?

best substrate

-

?

digallate + H2O

gallate

-

-

?

-

substrate: tannins

i.e. 3,4,5-trihydroxybenzoate

?

-

-

?

-

substrate: tannins

i.e. 3,4,5-trihydroxybenzoate

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

650670

-

650670

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

substrate: tannins

i.e. 3,4,5-trihydroxybenzoate

?

-

-

?

-

substrate: tannins

i.e. 3,4,5-trihydroxybenzoate

?

-

-

?

Bos taurus

-

-

?

-

-

?

Cryphonectria parasitica

-

-

?

-

-

?

Fusarium solani

-

-

?

Hyalopus sp.

-

-

?

-

650689

-

650689

?

-

-

?

-

665236

-

?

-

728909

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

epicatechin gallate + H2O

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

epicatechin gallate + H2O

gallate + epicatechin

-

best substrate

-

?

complete hydrolysis within 40 min

-

?

epicatechin gallate + H2O

gallic acid + epicatechin

Aspergillus ficuum

-

692431, 693962

-

?

-

-

?

-

-

?

epigallocatechin gallate + H2O

epigallocatechin + gallate

-

-

?

-

-

?

epigallocatechin gallate + H2O

gallate + epigallocatechin

-

-

?

-

-

?

-

-

?

-

-

?

-

730080, 730219

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

Hyalopus sp.

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

730219, 728909, 729503

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

complete hydrolysis within 40 min

-

?

-

-

?

-

-

?

epigallocatechin gallate + H2O

gallic acid + epigallocatechin

Aspergillus ficuum

-

692431, 693962

-

?

-

-

?

-

-

?

-

692421

-

?

-

-

?

Lactobacillus plantarum CECT 748 / ATCC 14917 / DSM 20174

-

-

?

epigallocatechin-3-O-(3-O-methyl) gallate + H2O

?

-

-

?

-

-

?

-

-

?

ethyl 3,4-dihydroxybenzoate + H2O

ethanol + 3,4-dihydroxybenzoate

-

-

?

-

-

?

-

-

?

-

-

?

ethyl 3,5-dihydroxybenzoate + H2O

ethanol + 3,5-dihydroxybenzoate

-

-

?

-

-

?

ethyl 4-hydroxybenzoate + H2O

ethanol + 4-hydroxybenzoate

-

-

?

-

-

?

-

-

?

-

-

?

ethyl benzoate + H2O

ethanol + benzoate

-

-

?

-

-

?

-

-

?

-

-

?

ethyl gallate + H2O

gallate + ethanol

-

worst substrate

-

?

-

729042, 730214

-

?

-

-

?

ethyl gallate + H2O

gallic acid + ethanol

-

55% activity compared to methyl gallate

-

?

-

55% activity compared to methyl gallate

-

?

-

-

?

Lactobacillus plantarum CECT 748 / ATCC 14917 / DSM 20174

-

-

?

-

-

?

ferulic ethyl ester + H2O

ethanol + ferulate

-

-

?

-

-

?

ferulic methyl ester + H2O

methanol + ferulate

-

-

?

-

-

?

gallocatechin gallate + H2O

?

-

-

?

-

-

?

-

-

?

gallocatechin gallate + H2O

gallic acid + gallocatechin

-

best substrate

-

?

-

-

?

-

-

?

Lactobacillus plantarum CECT 748 / ATCC 14917 / DSM 20174

-

-

?

gallotannin + H2O

gallate + D-glucose

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

Hyalopus sp.

-

-

?

-

730219, 728909

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

gallotannin + H2O

gallic acid + D-glucose

Aspergillus fischeri

-

-

?

-

hydrolysis of ester and depside linkages of gallotannins via galloylglucose

-

?

-

680530, 690499

-

?

-

hydrolysis of ester and depside linkages of gallotannins via galloylglucose

-

?

Aspergillus niger Aa-20

-

-

?

-

hydrolysis of ester and depside linkages of gallotannins via galloylglucose

-

?

Aspergillus sp.

-

-

?

-

hydrolysis of ester and depside linkages of gallotannins via galloylglucose

-

?

-

-

?

-

hydrolysis of ester and depside linkages of gallotannins via galloylglucose

-

?

-

-

?

-

hydrolysis of ester and depside linkages of gallotannins via galloylglucose

-

?

-

692421

-

?

Fusarium solani

-

-

?

-

hydrolysis of ester and depside linkages of gallotannins via galloylglucose

-

?

-

-

?

-

hydrolysis of ester and depside linkages of gallotannins via galloylglucose

-

?

Libidibia coriaria

-

-

?

-

hydrolysis of ester and depside linkages of gallotannins via galloylglucose

-

?

Penicillium sp.

-

-

?

-

hydrolysis of ester and depside linkages of gallotannins via galloylglucose

-

?

Rhizopus sp.

-

690499

-

?

Rhizopus sp. C3-I

-

690499

-

?

-

-

?

-

hydrolysis of ester and depside linkages of gallotannins via galloylglucose

-

?

lauryl gallate + H2O

gallate + lauric acid

-

-

?

-

-

?

meta-digallic acid + H2O

gallic acid

-

94257

-

?

-

-

?

methyl 2,4-dihydroxybenzoate + H2O

methanol + 2,4-dihydroxybenzoate

-

-

?

-

-

?

methyl 3,4,5-trihydroxybenzoate + H2O

methanol + 3,4,5-trihydroxybenzoate

-

709504

-

?

-

709504

-

?

methyl 4-hydroxybenzoate + H2O

methanol + 4-hydroxybenzoate

-

-

?

-

-

?

methyl benzoate + H2O

methanol + benzoate

-

-

?

-

-

?

-

-

?

-

-

?

methyl gallate + 1-propanol

propyl gallate + ?

-

transesterification, synthetic yields of 45% are obtained with 30% of 1-propanol at pH 5.0

-

?

Lactobacillus plantarum CECT 748 / ATCC 14917 / DSM 20174

-

transesterification, synthetic yields of 45% are obtained with 30% of 1-propanol at pH 5.0

-

?

methyl gallate + H2O

gallate + methanol

-

-

?

-

-

?

-

-

?

best substrate

Aspergillus awamori BTMFW032

-

?

best substrate

-

?

-

715957, 730219

-

?

-

-

?

-

713803, 713805, 714873, 730219

-

?

-

-

?

-

713803, 714873, 730219

-

?

-

Aspergillus niger MTCC 2425

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

691564

-

r

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

Bacillus subtilis

-

-

?

-

-

?

-

713919, 730219

-

?

-

-

?

Enterobacter cloacae

-

-

?

-

-

?

-

-

?

Enterobacter sp. KPJ03

-

-

?

Escherichia coli

-

-

?

Hyalopus sp.

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

680231, 715076, 715220, 729042, 729413, 729424, 730219, 728909, 729503, 730214, 730911

-

?

-

recombinant tannase shows similar affinity for both methyl gallate and ethyl gallate

-

?

-

-

?

-

-

?

Lactobacillus plantarum CECT 748 / ATCC 14917 / DSM 20174

-

680231

-

?

-

recombinant tannase shows similar affinity for both methyl gallate and ethyl gallate

-

?

-

Lactobacillus plantarum CIR1

-

?

-

729413

-

?

Lactobacillus plantarum SN35N

Lysinibacillus sphaericus

-

730911

-

?

-

715681, 729424

-

?

-

-

?

-

-

?

Rhodococcus sp.

-

-

?

-

-

?

Serratia ficaria

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

methyl gallate + H2O

gallic acid + methanol

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

665026, 665888

-

?

-

94249, 94250, 94257

-

?

-

-

?

-

-

?

Bos taurus

-

-

?

Cryphonectria parasitica

-

-

?

-

-

?

-

695165

-

?

-

-

?

-

-

?

Rhizopus oryzae

-

-

?

-

94258

-

?

-

-

?

-

-

?

-

-

?

methyl gallate + H2O

methanol + gallate

Aspergillus fischeri

-

-

?

Aspergillus sp.

-

-

?

Fusarium solani

-

-

?

-

-

?

methyl gentisate + H2O

methanol + gentisate

-

-

?

-

-

?

methyl salicylate + H2O

methanol + salicylate

-

-

?

-

-

?

methyl vanillate + H2O

methanol + vanillate

-

-

?

-

-

?

-

-

?

-

-

?

n-propyl gallate + H2O

gallic acid + n-propanol

-

55% activity compared to methyl gallate

-

?

-

55% activity compared to methyl gallate

-

?

-

-

?

-

-

?

-

-

?

propyl 4-hydroxybenzoate + H2O

propanol + 4-hydroxybenzoate

-

-

?

-

-

?

propyl gallate + H2O

gallate + 1-propanol

-

-

?

Lactobacillus plantarum CECT 748 / ATCC 14917 / DSM 20174

-

-

?

propyl gallate + H2O

gallate + propanol

-

Aspergillus awamori BTMFW032

-

?

-

-

?

-

715957, 729469

-

?

-

-

Aspergillus niger MTCC 2425

?

-

730351

-

?

-

-

?

Aspergillus niger T3-5-1

-

730351

-

?

-

-

?

P78581

-

692434

-

r

-

-

?

-

-

?

-

730190

-

?

46% hydrolysis within 40 min

-

?

protocatechuic acid ethyl ester + H2O

ethanol + protocatechuate

-

-

?

-

-

?

tannic acid + 1-propanol

propyl gallate + ?

-

transesterification, synthetic yields of 45% are obtained with 30% of 1-propanol at pH 5.0

-

?

Lactobacillus plantarum CECT 748 / ATCC 14917 / DSM 20174

-

transesterification, synthetic yields of 45% are obtained with 30% of 1-propanol at pH 5.0

-

?

tannic acid + 10 H2O

10 gallic acid + glucose

-

-

?

-

-

?

-

653585

-

653585

?

-

gallotannin from fruits of Terminalia chebula

650669

-

650669

?

tannic acid + H2O

10 gallate + D-glucose

-

enzyme hydrolyzes the ester and depside linkages of tannic acid

-

?

-

enzyme hydrolyzes the ester and depside linkages of tannic acid

-

?

-

-

?

-

the enzyme plays an important role in the complex tannin formation in plants and is involved in fruit ripening

-

?

-

-

?

-

94253

-

94253

?

-

-

?

-

the enzyme plays an important role in the complex tannin formation in plants and is involved in fruit ripening

-

?

-

-

?

-

the enzyme plays an important role in the complex tannin formation in plants and is involved in fruit ripening

-

?

-

-

?

-

-

?

-

-

?

-

best substrate

-

?

-

the enzyme plays an important role in the complex tannin formation in plants and is involved in fruit ripening

-

Aspergillus niger MTCC 2425

?

-

best substrate

-

?

-

-

?

-

-

?

-

94256

-

94256

?

-

94257

-

94257

?

-

-

?

-

the enzyme plays an important role in the complex tannin formation in plants and is involved in fruit ripening

-

?

-

-

?

-

specific for

?

-

-

?

-

-

?

-

-

?

-

-

?

-

the enzyme plays an important role in the complex tannin formation in plants and is involved in fruit ripening

-

Candida sp. (in: Saccharomycetales)

?

-

-

?

-

-

?

Corynebacterium sp. Q40

-

-

?

Cryphonectria parasitica

-

-

?

-

the enzyme plays an important role in the complex tannin formation in plants and is involved in fruit ripening

-

?

-

-

?

-

the enzyme plays an important role in the complex tannin formation in plants and is involved in fruit ripening

-

?

-

-

Klebsiella pneumoniae Q52

?

-

-

?

Paenibacillus polymyxa

-

-

Paenibacillus polymyxa Q47

?

-

-

?

-

-

?

-

the enzyme plays an important role in the complex tannin formation in plants and is involved in fruit ripening

-

?

-

-

?

-

the enzyme plays an important role in the complex tannin formation in plants and is involved in fruit ripening

-

?

-

-

?

-

the enzyme plays an important role in the complex tannin formation in plants and is involved in fruit ripening

-

?

tannic acid + H2O

?

-

-

Aspergillus flavus

-

94250, 94251, 94253

-

Aspergillus niger PKL 104

-

94250, 94254, 94259

-

Aspergillus niger LCF 8

-

94259

-

-

94254

-

-

94249, 94250, 94252, 94256, 94257, 94260

-

Candida sp. (in: Saccharomycetales)

-

-

-

-

-

-

-

-

-

-

-

94247, 94248

-

Candida sp. (in: Saccharomycetales) K-1

-

94247

-

Corynebacterium sp.

-

-

Corynebacterium sp. Q40

-

-

-

Klebsiella pneumoniae Q52

-

-

-

Paenibacillus polymyxa

-

Paenibacillus polymyxa Q47

-

-

-

Penicillium chrysogenum NCIM-722

-

94245, 94250

-

-

94245

-

Penicillium expansum

-

-

Penicillium javanicum

-

-

-

-

-

-

-

94258

-

tannic acid + H2O

gallate + ?

Aspergillus flavus

-

-

?

Aspergillus foetidus

-

Aspergillus foetidus GMRB 013 MTCC 3557

-

?

-

-

?

-

-

?

Aspergillus sp.

-

-

?

-

-

?

-

-

?

-

679481

-

?

Fusarium sp.

-

-

?

-

680231

-

?

Lactobacillus plantarum CECT 748 / ATCC 14917 / DSM 20174

-

680231

-

?

Penicillium sp.

-

-

?

Rhizopus oryzae

-

Rhizopus oryzae RO IIT RB-13

-

?

-

-

?

-

-

?

-

tannins from Chebulina myrobalan

-

?

-

-

?

-

tannins from Chebulina myrobalan

-

?

Trichoderma sp.

-

-

?

-

677782

-

?

tannic acid + H2O

gallate + D-glucose

-

-

?

-

-

?

-

Aspergillus ficuum Gim 3.6

-

?

Aspergillus ficuum

-

730801

-

?

-

from green tea leaves

682610

-

?

-

730801

-

?

Aspergillus foetidus

-

691971

-

?

Aspergillus foetidus MTCC 6322

-

691971

-

?

-

729469, 730219

-

?

-

713803, 730080, 730219, 730351

-

?

-

-

?

-

713803, 730219

-

?

-

-

?

Aspergillus niger T3-5-1

-

730351

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

691564, 730219

-

?

-

-

?

-

-

?

-

-

?

-

680410, 730219

-

?

-

Bacillus licheniformis KBR6

-

?

-

680410

-

?

Bacillus subtilis

-

-

?

-

-

?

-

692421, 730219

-

?

Enterobacter cloacae

-

-

?

-

-

?

Escherichia coli

-

-

?

Hyalopus sp.

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

-

?

-

729042, 729413, 729424, 730219, 728909, 729503, 730214, 730911

-

?

-

-

?

-

Lactobacillus plantarum CIR1

-

?

-

729413

-

?

Lactobacillus plantarum SN35N

Lysinibacillus sphaericus

-

730911

-

?

-

-

?

-

-

?

-

-

?

Penicillium sp.

-

-

?

Penicillium sp. EZ-ZH190

-

-

?

Penicillium sp. EZ-ZH290

-

-

?

Penicillium sp. EZ-ZH390

-

-

?

Rhodococcus sp.

-

-

?

-

-

?

Serratia ficaria

-

-

?

-

-

?

-

-

?

-

best substrate

-

?

-

best substrate

-

?

-

-

?

-

730190

-

?

complete hydrolysis within 40 min

-

?

-

-

?

-

-

?

tannic acid + H2O

gallic acid + ?

Aspergillus aculeatus DBF9

-

677408

-

?

-

677408

-

?

-

-

?

Aspergillus flavus

-

94250, 94251, 94253

-

?

Aspergillus niger PKL 104

-

94250, 94254, 94259, 682999

-

?

Aspergillus niger LCF 8

-

94259

-

?

-

94254

-

?

-

94250, 94252, 94256, 94257, 94260

-

?

-

130% activity compared to methyl gallate

-

?

-

130% activity compared to methyl gallate

-

?

-

94252

-

?

Aspergillus sojae

-

-

?

Aspergillus usamii

-

-

?

Aspergillus ustus

-

Aureobasidium pullulans DBS66

-

?

Aureobasidium pullulans

-

681345

-

?

-

681345

-

?

Bacillus pumilus

-

-

?

Candida sp. (in: Saccharomycetales)

-

663739

-

?

-

94247, 94248

-

?

Candida sp. (in: Saccharomycetales) K-1

-

94247

-

?

Corynebacterium sp.

-

-

?

Corynebacterium sp. Q40

-

-

?

-

Klebsiella pneumoniae Q52

-

?

-

-

?

Paenibacillus polymyxa

-

Paenibacillus polymyxa Q47

-

?

-

-

?

Penicillium chrysogenum NCIM-722

-

94245, 94250

-

?

-

94245

-

?

Penicillium expansum

-

-

?

Penicillium javanicum

-

-

?

Penicillium oxalicum

-

-

?

-

-

?

-

94258

-

?

tannic acid + H2O

gallic acid + D-glucose

-

707202

-

?

lowest activity

Aspergillus awamori BTMFW032

-

?

lowest activity

Aspergillus awamori MTCC9299

-

?

-

707202

-

?

Aspergillus heteromorphus

-

714559

-

?

Aspergillus heteromorphus MTCC 8818

-

714559

-

?

-

highest rate of tannase activity at 1.5-2.5% (w/v) tannic acid

714594

-

?

-

715957

-

?

-

709504, 713805, 714873

-

?

-

714873, 709504

-

?

-

707808

-

?

-

highest rate of tannase activity at 1.5-2.5% (w/v) tannic acid

714594

-

?

Aspergillus tamarii IMI388810 (B)

-

707018

-

?

-

707018

-

?

Bacillus licheniformis KBR6

-

709505

-

?

-

709505

-

?

-

-

?

-

-

?

Enterobacter sp. KPJ03

-

-

?

Fusarium subglutinans

-

709672

-

?

Hyalopus sp.

-

709331

-

?

Hyalopus sp. DSF3

-

709331

-

?

-

Lysinibacillus sphaericus

-

?

-

at least 95% of tannic acid is transformed into pure gallate

715220

-

?

-

715681

-

?