Chlorophyllase has been found in higher plants, diatoms, and in the green algae Chlorella . This enzyme forms part of the chlorophyll degradation pathway and is thought to take part in de-greening processes such as fruit ripening, leaf senescence and flowering, as well as in the turnover and homeostasis of chlorophyll . This enzyme acts preferentially on chlorophyll a but will also accept chlorophyll b and pheophytins as substrates . Ethylene and methyl jasmonate, which are known to accelerate senescence in many species, can enhance the activity of the hormone-inducible form of this enzyme .
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
chlorophyll chlorophyllidohydrolase
Chlorophyllase has been found in higher plants, diatoms, and in the green algae Chlorella [3]. This enzyme forms part of the chlorophyll degradation pathway and is thought to take part in de-greening processes such as fruit ripening, leaf senescence and flowering, as well as in the turnover and homeostasis of chlorophyll [4]. This enzyme acts preferentially on chlorophyll a but will also accept chlorophyll b and pheophytins as substrates [5]. Ethylene and methyl jasmonate, which are known to accelerate senescence in many species, can enhance the activity of the hormone-inducible form of this enzyme [5].
the enzyme is involved in the first step of chlorophyll degradation, molecular regulation of in vivo activities, AtCLH2 might play a distinctive role in chlorophyll catabolism in vivo, overview
the enzyme is involved in the first step of chlorophyll degradation, molecular regulation of in vivo activities, AtCLH2 might play a distinctive role in chlorophyll catabolism in vivo, overview
chlorophyll breakdown is indistinguishable between wild-type plants and clh1 and clh1/clh2 mutant plants. By contrast, chlorophyll degradation is significantly delayed in an Arabidopsis thaliana mutant that lacks pheophytinase (PPH), catalyzing the breakdown of phytol and known to be associated with chlorophyll catabolism. These data indicate that PPH, not CLH1, is responsible for the majority of methyl jasmonate-enhanced chlorophyll breakdown, even though CLH1 is highly induced by methyl jasmonate
chlorophyll breakdown is indistinguishable between wild-type plants and clh1 and clh1/clh2 mutant plants. By contrast, chlorophyll degradation is significantly delayed in an Arabidopsis thaliana mutant that lacks pheophytinase (PPH), catalyzing the breakdown of phytol and known to be associated with chlorophyll catabolism. These data indicate that PPH, not CLH1, is responsible for the majority of MeJA-enhanced chlorophyll breakdown, even though CLH1 is highly induced by methyl jasmonate
chlorophyllase 1 (CLH1) is not involved in endogenous chlorophyll catabolism, but CLH1 promotes chlorophyllide formation upon disruption of leaf cells, or when it is artificially mistargeted to the chloroplast. CLH is responsible for chlorophyllide formation after the collapse of cells, suggesting that chlorophyllide formation might be a process of defense against chewing herbivores. Arabidopsis leaves with genetically enhanced CLH1 activity exhibit toxicity when fed to Spodoptera litura larvae, an insect herbivore. Purified chlorophyllide partially suppresses the growth of the larvae. Isozyme CLH1 represents the majority of detectable CLH activity in Arabidopsis thaliana
construction of T-DNA insertion clh1 and clh2 single and double knockout lines, which are still able to degrade chlorophyll during senescence, phenotypes, overview
construction of T-DNA insertion clh1 and clh2 single and double knockout lines, which are still able to degrade chlorophyll during senescence, phenotypes, overview
construction of T-DNA insertion clh1 and clh2 single and double knockout lines, which are still able to degrade chlorophyll during senescence, phenotypes, overview
inhibition of expression of AtCLH2 by RNA interference, transfection using Agrobacterium tumefaciens strain LBA4404, AtCLH2 RNAi plants show decreased contents of chlorophyllide without a substantial change in the total amount of the extractable chlorophyll and consequently presented lower chlorophyllide to chlorophyll ratios in their leaves, phenotype, overview
construction of clh1 and clh1/clh2 mutant plants as transfer DNA insertion lines. Methyl jasmonate promotes chlorophyll degradation in the clh mutants as well as in the wild-type, phenotypes, overview
construction of clh1 and clh1/clh2 mutant plants as transfer DNA insertion lines. Methyl jasmonate promotes chlorophyll degradation in the clh mutants as well as in the wild-type, phenotypes, overview
gene ATHCOR1: construction of transgenic Arabidopsis thaliana plants overexpressing the enzyme via infection with Agrobacterium tumefaciens and transformation, sense and antisense orientation, the sense mutation changed the chlorophyll a to chlorophyll b ratio, overview, gene AtCLH2: cloning and analysis
construction of T-DNA insertion clh1 and clh2 single and double knockout lines, which are still able to degrade chlorophyll during senescence, phenotypes, overview
construction of T-DNA insertion clh1 and clh2 single and double knockout lines, which are still able to degrade chlorophyll during senescence, phenotypes, overview
construction of T-DNA insertion clh1 and clh2 single and double knockout lines, which are still able to degrade chlorophyll during senescence, phenotypes, overview
construction of clh1 and clh1/clh2 mutant plants as transfer DNA insertion lines. Methyl jasmonate promotes chlorophyll degradation in the clh mutants as well as in the wild-type, phenotypes, overview
construction of clh1 and clh1/clh2 mutant plants as transfer DNA insertion lines. Methyl jasmonate promotes chlorophyll degradation in the clh mutants as well as in the wild-type, phenotypes, overview
development of micellar electrokinetic chromatography (MEKC) assay for the plant membrane enzyme chlorophyllase, evaluation of several different permanently and dynamically coated capillaries
24 h exposure at 4°C followed by 10 d of recovery, leads to overall reduced enzyme activity with the eti5 mutant plants being more sensitive, during the stress time and the first day of recovery the enzyme activity is highly reduced, the enzyme activity is more affected during the experiment time in wild-type plants, overview
24 h exposure at 4°C followed by 10 d of recovery, leads to overall reduced enzyme activity with the eti5 mutant plants being more sensitive, during the stress time and the first day of recovery the enzyme activity is highly reduced, the enzyme activity is more affected during the experiment time in wild-type plants, overview
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene chl2, DNA and amino acid sequence determination and anaylsis, transient expression of isozyme AtCLH2 as GFP-tagged protein, e.g. in senescent mesophyll protoplasts, expression analysis
expression of gene ATHCOR1 in Escherichia coli BL21 as maltose-binding protein fusion protein, cloning of the gene into an expression vector for transformation of Arabidopsis thaliana plants
gene chl1, DNA and amino acid sequence determination and anaylsis, transient expression of isozyme AtCLH1 as GFP-tagged protein, e.g. in senescent mesophyll protoplasts, expression analysis
gene clh1, recombinant expression of YFP-tagged enzyme in Arabidopsis thaliana, the YFP-tagged enzyme is detected outside of chloroplasts in leaf protoplasts prepared from plants constitutively expressing CLH1-YFP