Information on EC 3.1.1.11 - pectinesterase and Organism(s) Arabidopsis thaliana and UniProt Accession O80722

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This record set is specific for:
Arabidopsis thaliana
UNIPROT: O80722


The expected taxonomic range for this enzyme is: Eukaryota, Bacteria


The taxonomic range for the selected organisms is: Arabidopsis thaliana

EC NUMBER
COMMENTARY hide
3.1.1.11
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RECOMMENDED NAME
GeneOntology No.
pectinesterase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
pectin + n H2O = n methanol + pectate
show the reaction diagram
reaction mechanism, D157, stabilized by a hydrogen bond to R225, performs a nucleophilic attack on the ester bond of the carboxymethyl group of HGA
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of carboxylic ester
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
pectin degradation I
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pectin degradation II
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Pentose and glucuronate interconversions
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
pectin pectylhydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
9025-98-3
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
pectin + H2O
methanol + pectate
show the reaction diagram
pectin + H2O
methanol + pectate
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
pectin + H2O
methanol + pectate
show the reaction diagram
O23447, O80722, Q5MFV6, Q5MFV8, Q84WM7, Q8GXA1, Q8L7Q7, Q9LSP1, Q9LY18, Q9LY19, Q9SMY6
PME plays an important role in elongation of the pollen tube in pistil, which is essential for delivering sperms into the female gametophyte in sexual plant reproduction, regulation mechanism, overview
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?
pectin + H2O
methanol + pectate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
aluminium
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a toxic metal in soils that inhibits plant root elongation, can be modulated by PME activity, overexpression of PME activity leads to increases in aluminium content in the plant, which correlates to reductions in the degree of pectin methylesterification, overview
additional information
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the enzyme does not require salt for activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NaCl
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activity decreases in the presence of 0.1 M NaCl and is 4times lower in 0.5 M NaCl
additional information
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.246
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crude extract
11.98
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purified enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8 - 8.5
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in phosphate buffer
9
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around pH 9 in glycine buffer
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9.6
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isoelectric focusing
9.8
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calculated from amino acid sequence
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
tube, the enzyme belongs to the group II of pectinesterases in Arabidopsis thaliana pollen
Manually annotated by BRENDA team
additional information
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isoform PME3 is ubiquitously expressed in Arabidopsis thaliana, particularly in vascular tissues
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
UNIPROT
ORGANISM
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
33300
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x * 33300, mature enzyme, estimated from SDS-PAGE
35000
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SDS-PAGE
37000
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SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 33300, mature enzyme, estimated from SDS-PAGE
additional information
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primary structures of isozymes, structural and processing motifs, three-dimensional structure analysis, overview
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
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the enzyme contains a pro-region, role of the PRO region in PME targeting and function
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9
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no activity at pH 9
683188
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 55
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incubation at 30C has little but significant effect on enzyme activity, the incubation at 55C abolishes over 95% of enzyme activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, crude extract, several weeks, no significant loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation and Ni-NTA agarose column chromatography
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Ni-NTA His-bind resin chromatography, DEAE-Sephadex gel filtration, CM-cellulose column chromatography, and Shephacryl SH-100 gel filtration
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA sequence and phylogenetic analysis
diverse isozymes, DNA sequence anaylsis, phylogenetic tree, PME transcriptomes, overview
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DNA sequence and phylogenetic analysis
expressed in Escherichia coli strain JM101, IPTG induction at 37C instead of 25-28C results in approximately 10times less PME activity in the extract
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expressed in Escherichia coli strain M15, the recombinant PME proteins (full-length and mature) do not show either PME or RIP activity
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YFP-tagged protein expressed in host
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
isoform PME3 is induced upon infection with Botrytis cinerea and Pectobacterium carotovorum. The enzyme expression is induced upon herbivore attack as a defense mechanism
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upon infection, Pectobacterium carotovorum and Botrytis cinerea induce in Arabidopsis a rapid expression of isoform PME3
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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the degree of methylesterification of galacturonic acids is affected in the pme3-1 mutant enzyme