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Information on EC 3.1.1.1 - carboxylesterase and Organism(s) Mus musculus and UniProt Accession Q8VCT4

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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.1 carboxylesterase
IUBMB Comments
Wide specificity. The enzymes from microsomes also catalyse the reactions of EC 3.1.1.2 (arylesterase), EC 3.1.1.5 (lysophospholipase), EC 3.1.1.6 (acetylesterase), EC 3.1.1.23 (acylglycerol lipase), EC 3.1.1.28 (acylcarnitine hydrolase), EC 3.1.2.2 (palmitoyl-CoA hydrolase), EC 3.5.1.4 (amidase) and EC 3.5.1.13 (aryl-acylamidase). Also hydrolyses vitamin A esters.
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Mus musculus
UNIPROT: Q8VCT4
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Word Map
The taxonomic range for the selected organisms is: Mus musculus
The enzyme appears in selected viruses and cellular organisms
Synonyms
esterase, carboxylesterase, butyrate esterase, carboxyl esterase, carboxylesterase 1, egasyn, serine protease-like, hce-2, acyl coenzyme a:cholesterol acyltransferase, esterase a, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
mouse carboxylesterase
-
ACAT
-
-
-
-
Acyl coenzyme A:cholesterol acyltransferase
-
-
-
-
acylcarnitine hydrolase
-
-
ali-esterase
-
-
-
-
aliesterase
-
-
-
-
alpha-carboxylesterase
-
-
-
-
B-esterase
-
-
-
-
Brain carboxylesterase hBr1
-
-
-
-
butyrate esterase
-
-
-
-
butyryl esterase
-
-
-
-
CaE
-
-
-
-
carboxyesterase
-
-
-
-
Carboxyesterase ES-10
-
-
-
-
carboxyl ester hydrolase
-
-
-
-
carboxylate esterase
-
-
-
-
carboxylesterase 1C
-
Carboxylesterase-5C
-
-
-
-
carboxylic acid esterase
-
-
-
-
carboxylic ester hydrolase
-
-
-
-
carboxylic esterase
-
-
-
-
Carboxylic-ester hydrolase
-
-
-
-
CES1
-
isozyme
CES2
-
isozyme
cocaine esterase
-
-
-
-
Egasyn
-
-
-
-
Es-22
-
-
-
-
ES-HTEL
-
-
-
-
ES-HVEL
-
-
-
-
ES-Male
-
-
-
-
ES-THET
-
-
-
-
EST-5A
-
-
-
-
EST-5B
-
-
-
-
EST-5C
-
-
-
-
esterase 1F
-
-
esterase 2B
-
-
esterase 6A
-
-
esterase 9A
-
-
esterase A
-
-
-
-
esterase B
-
-
-
-
esterase D
-
-
-
-
esterase, carboxyl
-
-
-
-
Esterase-22
-
-
-
-
Esterase-31
-
-
-
-
HMSE
-
-
-
-
Kidney microsomal carboxylesterase
-
-
-
-
Liver microsomal carboxylesterase
-
-
-
-
methylbutyrase
-
-
-
-
methylbutyrate esterase
-
-
-
-
Microsomal palmitoyl-CoA hydrolase
-
-
-
-
monobutyrase
-
-
-
-
Monocyte/macrophage serine esterase
-
-
-
-
mouse carboxylesterase
-
Non-specific carboxylesterase
-
-
-
-
nonspecific carboxylesterase
-
-
-
-
PI 5.5 esterase
-
-
-
-
PI 6.1 esterase
-
-
-
-
procaine esterase
-
-
-
-
Proline-beta-naphthylamidase
-
-
-
-
propionyl esterase
-
-
-
-
serine protease-like
-
triacetin esterase
-
-
-
-
vitamin A esterase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of carboxylic ester
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -
SYSTEMATIC NAME
IUBMB Comments
carboxylic-ester hydrolase
Wide specificity. The enzymes from microsomes also catalyse the reactions of EC 3.1.1.2 (arylesterase), EC 3.1.1.5 (lysophospholipase), EC 3.1.1.6 (acetylesterase), EC 3.1.1.23 (acylglycerol lipase), EC 3.1.1.28 (acylcarnitine hydrolase), EC 3.1.2.2 (palmitoyl-CoA hydrolase), EC 3.5.1.4 (amidase) and EC 3.5.1.13 (aryl-acylamidase). Also hydrolyses vitamin A esters.
CAS REGISTRY NUMBER
COMMENTARY hide
9016-18-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-methylumbelliferyl acetate + H2O
4-methylumbelliferol + acetate
show the reaction diagram
-
-
-
?
(2R)-fenvalerate + H2O
?
show the reaction diagram
-
-
-
?
(2S)-fenvalerate + H2O
?
show the reaction diagram
-
-
-
?
(alphaR)(2R)-fenvalerate + H2O
?
show the reaction diagram
-
-
-
?
(alphaR)(2S)-fenvalerate + H2O
?
show the reaction diagram
-
-
-
?
(alphaS)(2R)-fenvalerate + H2O
?
show the reaction diagram
-
-
-
?
(S)-1-(6-fluoro-2-methyl-3,4-dihydroquinolin-1(2H)-yl)-2-(isoquinolin-5-yloxy)ethanone + H2O
(2S)-6-fluoro-2-methyl-1,2,3,4-tetrahydroquinoline + (isoquinolin-5-yloxy)acetic acid
show the reaction diagram
4-methylumbelliferyl acetate + H2O
4-methylumbelliferol + acetate
show the reaction diagram
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
show the reaction diagram
4-nitrophenyl hexanoate + H2O
4-nitrophenol + hexanoate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl propionate + H2O
4-nitrophenol + propionate
show the reaction diagram
-
-
-
-
?
alpha-cypermethrin + H2O
?
show the reaction diagram
-
-
-
?
alpha-naphthyl acetate + H2O
alpha-naphthol + acetate
show the reaction diagram
-
-
-
?
Amplex red + H2O
resorufin + acetate
show the reaction diagram
Amplex red is readily converted to resorufin by a carboxylesterase without requiring H2O2, horseradish peroxidase, or oxygen
-
-
?
beta-naphthyl acetate + H2O
beta-naphthol + acetate
show the reaction diagram
-
-
-
?
capecitabin + H2O
?
show the reaction diagram
-
-
-
-
?
cilazapril + H2O
?
show the reaction diagram
-
-
-
-
?
cis-cypermethrin + H2O
?
show the reaction diagram
-
-
-
?
cis-permethrin + H2O
?
show the reaction diagram
-
-
-
?
cocaine + H2O
ecgonine methyl ester + benzoate
show the reaction diagram
CPT-11 + H2O
SN-38 + ?
show the reaction diagram
-
the substrate is more specific for isozyme CES2 than for CES1
-
-
?
cyano(6-methoxy-2-naphthyl)methyl 3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarboxylate + H2O
hydroxy(6-methoxy-2-naphthyl)acetonitrile + 3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarboxylic acid
show the reaction diagram
-
-
-
?
cyano(6-methoxy-2-naphthyl)methyl butanoate + H2O
hydroxy(6-methoxy-2-naphthyl)acetonitrile + butanoate
show the reaction diagram
-
-
-
?
cyano(6-methoxynaphthalen-2-yl)methyl (2S)-2-(4-chlorophenyl)-3-methylbutanoic acid + H2O
(2R)-2-(4-chlorophenyl)-3-methylbutanoic acid + hydroxy(6-methoxy-2-naphthyl)acetonitrile
show the reaction diagram
-
-
-
?
cypermethrin + H2O
?
show the reaction diagram
-
-
-
?
epsilon-cypermethrin + H2O
?
show the reaction diagram
-
-
-
?
heroin + H2O
6-monoacetylmorphine + acetate
show the reaction diagram
-
the substrate is more specific for isozyme CES2 than for CES1
-
-
?
imidapril + H2O
?
show the reaction diagram
-
the substrate is more specific for isozyme CES2 than for CES1
-
-
?
meperidine + H2O
?
show the reaction diagram
-
specific substrate for isozyme CES1
-
-
?
methylphenidate + H2O
?
show the reaction diagram
-
specific substrate for isozyme CES1
-
-
?
methylprednisolone 21-hemisuccinate + H2O
?
show the reaction diagram
-
the substrate is more specific for isozyme CES2 than for CES1
-
-
?
N-acetyl-DL-phenylalanine beta-naphthyl ester + H2O
N-acetyl-DL-phenylalanine + beta-naphthol
show the reaction diagram
NAPBNE, a chromogenic substrate of chymotrypsin-like enzymes
-
-
?
oseltamivir + H2O
?
show the reaction diagram
-
specific substrate for isozyme CES1
-
-
?
permethrin + H2O
?
show the reaction diagram
-
-
-
?
quinapril + H2O
?
show the reaction diagram
-
-
-
-
?
temocapril + H2O
?
show the reaction diagram
-
specific substrate for isozyme CES1
-
-
?
trans-cypermethrin + H2O
?
show the reaction diagram
-
-
-
?
trans-permethrin + H2O
?
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(S)-1-(6-fluoro-2-methyl-3,4-dihydroquinolin-1(2H)-yl)-2-(isoquinolin-5-yloxy)ethanone + H2O
(2S)-6-fluoro-2-methyl-1,2,3,4-tetrahydroquinoline + (isoquinolin-5-yloxy)acetic acid
show the reaction diagram
-
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
bis(4-nitrophenyl) phosphate
-
-
bis(p-nitrophenyl) phosphate
-
a specific inhibitor of carboxylesterases
bis-4-nitrophenyl phosphate
BNPP
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
di(2-ethylhexyl)phthalate
-
induced by di(2-ethylhexyl)phthalate
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.004 - 0.077
4-nitrophenyl hexanoate
0.9
4-nitrophenyl propionate
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
94
4-nitrophenyl acetate
pH 8.0, 30°C
12 - 31
4-nitrophenyl hexanoate
230
4-nitrophenyl propionate
-
-
0.11
cyano(6-methoxy-2-naphthyl)methyl 3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarboxylate
pH 8.0, 30°C
0.099
cyano(6-methoxy-2-naphthyl)methyl butanoate
pH 8.0, 30°C
0.067
cyano(6-methoxynaphthalen-2-yl)methyl (2S)-2-(4-chlorophenyl)-3-methylbutanoic acid
pH 8.0, 30°C
0.12
cypermethrin
pH 8.0, 30°C
additional information
additional information
-
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00094
substrate (2S)-fenvalerate, pH 8.0, 30°C
0.0014
substrate (alphaR)(2S)-fenvalerate, pH 8.0, 30°C
0.0072
substrate (alphaS)(2R)-fenvalerate, pH 8.0, 30°C
0.02
substrate cyano(6-methoxynaphthalen-2-yl)methyl (2S)-2-(4-chlorophenyl)-3-methylbutanoic acid, pH 8.0, 30°C
0.027
substrate cis-permethrin, pH 8.0, 30°C
0.029
0.045
substrate alpha-cypermethrin, pH 8.0, 30°C
0.06
substrate cis-cypermethrin, pH 8.0, 30°C
0.061
substrate cyano(6-methoxy-2-naphthyl)methyl 3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarboxylate, pH 8.0, 30°C
0.075
substrate (alphaR)(2R)-fenvalerate, pH 8.0, 30°C
0.095
substrate epsilon-cypermethrin, pH 8.0, 30°C
0.239
substrate permethrin, pH 8.0, 30°C
0.246
substrate trans-cypermethrin, pH 8.0, 30°C
0.597
substrate trans-permethrin, pH 8.0, 30°C
15.9
-
-
17
substrate 4-nitrophenyl acetate, pH 8.0, 30°C
394.8
-
-
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.2
assay at
7.7 - 7.8
-
hydrolysis of 4-nitrophenyl hexanoate
8.6
-
hydrolysis of 4-nitrophenyl hexanoate
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 8.2
-
pH 7.0: about 75% of maximal activity, pH 8.2: about 95% of maximal activity, hydrolysis of p-nitrophenyl propionate
7.3 - 8.4
-
pH 7.3: about 95% of maximal activity, pH 8.4: about 80% of maximal activity
7.7 - 8.8
-
pH 7.7: about 75% of maximal activity, pH 8.8: about 90% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
very low activity
Manually annotated by BRENDA team
very low activity
Manually annotated by BRENDA team
additional information
no activity in heart, skeletal muscle, and testis
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
isoform-specific regulation of hepatic Ces mRNA expression and activity following administration of pharmacological activators of the constitutive androstane receptor (CAR), pregnane X receptor (PXR) and nuclear factor-E2-related protein (Nrf2) to mice, overview
evolution
CES belongs to the serine hydrolase family
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
EST1D_MOUSE
565
0
61788
Swiss-Prot
Secretory Pathway (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
184000
-
gel filtration
51000
-
gel filtration
56000
-
disc gel electrophoresis
58000
-
1 * 58000, SDS-PAGE
60200
-
3 * 60200, SDS-PAGE
75000
-
x * 75000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 58000, SDS-PAGE
trimer
-
3 * 60200, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
-
most CES isozymes are glycoproteins, and the carbohydrate chain is required for the enzyme activity
no modification
-
not a glycoprotein
side-chain modification
-
glycoprotein
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
stable to repeated thawing and refreezing
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70°C, stable for at least 3 months
-
4°C, up to 50% loss of activity within 3 h without glycerol, no loss of activity after 3 months in presence of 5% glycerol
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purification of a host chymotrypsin-like enzyme present on adult Schistosoma mansoni worms from infected mice, purification of the chymotrypsin-like enzyme from mouse blood plasma by SDS-PAGE
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
Nrf2 activator BHA, PXR ligand pregnenolone-16a-carbonitrile (PCN), and CAR activator 1,4-bis-[2-(3,5-dichloropyridyloxy)] benzene (TCPOBOP) induce Ces1d mRNA expression
CAR activator 1,4-bis-[2-(3,5-dichloropyridyloxy)] benzene (TCPOBOP) and Nrf2 activator BHA reduces expression of Ces1e
PXR ligand pregnenolone-16a-carbonitrile (PCN) and Nrf2 activator butylated hydroxyanisole (BHA) increase Ces1c expression
PXR ligand pregnenolone-16a-carbonitrile (PCN) and Nrf2 activator butylated hydroxyanisole (BHA) increase Ces1g expressio, while the CAR activator 1,4-bis-[2-(3,5-dichloropyridyloxy)] benzene (TCPOBOP) only slightly induces Ces1g expression
PXR ligand pregnenolone-16a-carbonitrile (PCN) increases Ces3a mRNA expression
PXR ligand pregnenolone-16a-carbonitrile (PCN) induces Ces1e expression
the CAR activator 1,4-bis-[2-(3,5-dichloropyridyloxy)] benzene (TCPOBOP) and the Nrf2 activator butylated hydroxyanisole (BHA) reduce Ces3a mRNA expression
the CAR activator 1,4-bis-[2-(3,5-dichloropyridyloxy)] benzene (TCPOBOP) slightly reduces Ces1c expression
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
-
the enzyme with substrate (S)-1-(6-fluoro-2-methyl-3,4-dihydroquinolin-1(2H)-yl)-2-(isoquinolin-5-yloxy)ethanone and inhibitor bis(p-nitrophenyl) phosphate are useful in a system to measure in plasma-free fraction for a plasma-labile compound
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Oehm, H.C.; de Looze, S.; Ronai, A.; von Deimling, O.
Purification and characterization of esterase 6A, a trimeric esterase of the house mouse (Mus musculus)
Eur. J. Biochem.
129
157-163
1982
Mus musculus
Manually annotated by BRENDA team
Otto, J.; Ronai, A.; von Deimling, O.
Purification and characterization of esterase 1F, the albumin esterase of the house mouse (Mus musculus)
Eur. J. Biochem.
116
285-291
1981
Mus musculus
Manually annotated by BRENDA team
Lexow, U.; Ronai, A.; von Deimling, O.
Purification and characterization of esterase 2B of the house mouse, Mus musculus
Eur. J. Biochem.
107
123-130
1980
Mus musculus
Manually annotated by BRENDA team
Goeppinger, A.; Riebschlaeger, M.; Ronai, A.; von Deimling, O.
Esterase XXVII. Purification and characterization of esterase-9A of mouse kidney
Biochim. Biophys. Acta
525
74-86
1978
Mus musculus
Manually annotated by BRENDA team
Stok, J.E.; Huang, H.; Jones, P.D.; Wheelock, C.E.; Morisseau, C.; Hammock, B.D.
Identification, expression, and purification of a pyrethroid-hydrolyzing carboxylesterase from mouse liver microsomes
J. Biol. Chem.
279
29863-29869
2004
Mus musculus, Mus musculus (Q8BK48)
Manually annotated by BRENDA team
Hosokawa, M.
Structure and catalytic properties of carboxylesterase isozymes involved in metabolic activation of prodrugs
Molecules
13
412-431
2008
Canis lupus familiaris, Cavia porcellus, Mesocricetus auratus, Mus musculus, Rattus norvegicus, Homo sapiens (P23141), Homo sapiens
Manually annotated by BRENDA team
Eng, H.; Niosi, M.; McDonald, T.S.; Wolford, A.; Chen, Y.; Simila, S.T.; Bauman, J.N.; Warmus, J.; Kalgutkar, A.S.
Utility of the carboxylesterase inhibitor bis-para-nitrophenylphosphate (BNPP) in the plasma unbound fraction determination for a hydrolytically unstable amide derivative and agonist of the TGR5 receptor
Xenobiotica
40
369-380
2010
Canis lupus familiaris, Homo sapiens, Mus musculus, Rattus norvegicus
Manually annotated by BRENDA team
Miwa, S.; Treumann, A.; Bell, A.; Vistoli, G.; Nelson, G.; Hay, S.; von Zglinicki, T.
Carboxylesterase converts Amplex red to resorufin implications for mitochondrial H2O2 release assays
Free Radic. Biol. Med.
90
173-183
2016
Mus musculus (Q8VCC2), Mus musculus C57BL/6 (Q8VCC2)
Manually annotated by BRENDA team
Baker, A.A.; Guo, G.L.; Aleksunes, L.M.; Richardson, J.R.
Isoform-specific regulation of mouse carboxylesterase expression and activity by prototypical transcriptional activators
J. Biochem. Mol. Toxicol.
29
545-551
2015
Mus musculus (P23953), Mus musculus (Q63880), Mus musculus (Q64176), Mus musculus (Q8VCC2), Mus musculus (Q8VCT4), Mus musculus, Mus musculus C57Bl6/J (P23953), Mus musculus C57Bl6/J (Q63880), Mus musculus C57Bl6/J (Q64176), Mus musculus C57Bl6/J (Q8VCC2), Mus musculus C57Bl6/J (Q8VCT4)
Manually annotated by BRENDA team
Igetei, J.E.; Liddell, S.; El-Faham, M.; Doenhoff, M.J.
Purification of a chymotrypsin-like enzyme present on adult Schistosoma mansoni worms from infected mice and its characterization as a host carboxylesterase
Parasitology
143
646-657
2016
Rattus norvegicus (P10959), Mus musculus (P23953), Mus musculus
Manually annotated by BRENDA team