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Information on EC 3.1.1.1 - carboxylesterase and Organism(s) Pseudomonas aeruginosa and UniProt Accession O33407

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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.1 carboxylesterase
IUBMB Comments
Wide specificity. The enzymes from microsomes also catalyse the reactions of EC 3.1.1.2 (arylesterase), EC 3.1.1.5 (lysophospholipase), EC 3.1.1.6 (acetylesterase), EC 3.1.1.23 (acylglycerol lipase), EC 3.1.1.28 (acylcarnitine hydrolase), EC 3.1.2.2 (palmitoyl-CoA hydrolase), EC 3.5.1.4 (amidase) and EC 3.5.1.13 (aryl-acylamidase). Also hydrolyses vitamin A esters.
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This record set is specific for:
Pseudomonas aeruginosa
UNIPROT: O33407
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The taxonomic range for the selected organisms is: Pseudomonas aeruginosa
The enzyme appears in selected viruses and cellular organisms
Synonyms
esterase, carboxylesterase, butyrate esterase, carboxyl esterase, carboxylesterase 1, egasyn, serine protease-like, hce-2, acyl coenzyme a:cholesterol acyltransferase, esterase a, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ACAT
-
-
-
-
Acyl coenzyme A:cholesterol acyltransferase
-
-
-
-
ali-esterase
-
-
-
-
aliesterase
-
-
-
-
alpha-carboxylesterase
-
-
-
-
B-esterase
-
-
-
-
Brain carboxylesterase hBr1
-
-
-
-
butyrate esterase
-
-
-
-
butyryl esterase
-
-
-
-
CaE
-
-
-
-
carboxyesterase
-
-
-
-
Carboxyesterase ES-10
-
-
-
-
carboxyl ester hydrolase
-
-
-
-
carboxylate esterase
-
-
-
-
Carboxylesterase-5C
-
-
-
-
carboxylic acid esterase
-
-
-
-
carboxylic ester hydrolase
-
-
-
-
carboxylic esterase
-
-
-
-
Carboxylic-ester hydrolase
-
-
-
-
CES
-
-
-
-
cocaine esterase
-
-
-
-
Egasyn
-
-
-
-
Es-22
-
-
-
-
ES-HTEL
-
-
-
-
ES-HVEL
-
-
-
-
ES-Male
-
-
-
-
ES-THET
-
-
-
-
EST-5A
-
-
-
-
EST-5B
-
-
-
-
EST-5C
-
-
-
-
esterase A
-
-
-
-
esterase B
-
-
-
-
esterase D
-
-
-
-
esterase, carboxyl
-
-
-
-
Esterase-22
-
-
-
-
Esterase-31
-
-
-
-
HMSE
-
-
-
-
Kidney microsomal carboxylesterase
-
-
-
-
Liver microsomal carboxylesterase
-
-
-
-
methylbutyrase
-
-
-
-
methylbutyrate esterase
-
-
-
-
Microsomal palmitoyl-CoA hydrolase
-
-
-
-
monobutyrase
-
-
-
-
Monocyte/macrophage serine esterase
-
-
-
-
Non-specific carboxylesterase
-
-
-
-
nonspecific carboxylesterase
-
-
-
-
PA3859
PI 5.5 esterase
-
-
-
-
PI 6.1 esterase
-
-
-
-
procaine esterase
-
-
-
-
Proline-beta-naphthylamidase
-
-
-
-
propionyl esterase
-
-
-
-
triacetin esterase
-
-
-
-
vitamin A esterase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of carboxylic ester
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -
SYSTEMATIC NAME
IUBMB Comments
carboxylic-ester hydrolase
Wide specificity. The enzymes from microsomes also catalyse the reactions of EC 3.1.1.2 (arylesterase), EC 3.1.1.5 (lysophospholipase), EC 3.1.1.6 (acetylesterase), EC 3.1.1.23 (acylglycerol lipase), EC 3.1.1.28 (acylcarnitine hydrolase), EC 3.1.2.2 (palmitoyl-CoA hydrolase), EC 3.5.1.4 (amidase) and EC 3.5.1.13 (aryl-acylamidase). Also hydrolyses vitamin A esters.
CAS REGISTRY NUMBER
COMMENTARY hide
9016-18-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
p-nitrophenyl palmitate
p-nitrophenol + palmitate
show the reaction diagram
-
-
-
?
1-naphthyl acetate + H2O
1-naphthol + acetate
show the reaction diagram
-
-
-
-
?
1-naphthyl butanoate + H2O
1-naphthol + butanoate
show the reaction diagram
-
54% of the activity with 1-naphthyl acetate
-
-
?
1-naphthyl caprylate + H2O
1-naphthol + caprylate
show the reaction diagram
-
14% of the activity with 1-naphthyl acetate
-
-
?
1-naphthyl laurate + H2O
1-naphthol + laurate
show the reaction diagram
-
9% of the activity with 1-naphthyl acetate
-
-
?
1-naphthyl oleate + H2O
1-naphthol + oleate
show the reaction diagram
-
2% of the activity with 1-naphthyl acetate
-
-
?
4-methylumbelliferyl acetate + H2O
4-methylumbelliferone + acetate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
show the reaction diagram
4-nitrophenyl butanoate + H2O
4-nitrophenol + butanoate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl butyrate + H2O
4-nitrophenol + butyrate
show the reaction diagram
-
-
-
?
4-nitrophenyl decanoate + H2O
4-nitrophenol + decanoate
show the reaction diagram
-
-
-
?
4-nitrophenyl heptanoate + H2O
4-nitrophenol + heptanoate
show the reaction diagram
-
-
-
?
4-nitrophenyl hexanoate + H2O
4-nitrophenol + hexanoate
show the reaction diagram
-
-
-
?
4-nitrophenyl laurate + H2O
4-nitrophenol + laurate
show the reaction diagram
-
-
-
?
4-nitrophenyl myristate + H2O
4-nitrophenol + myristate
show the reaction diagram
-
-
-
?
4-nitrophenyl nonanoate + H2O
4-nitrophenol + nonanoate
show the reaction diagram
-
-
-
?
4-nitrophenyl octanoate + H2O
4-nitrophenol + octanoate
show the reaction diagram
-
-
-
?
4-nitrophenyl palmitate + H2O
4-nitrophenol + palmitate
show the reaction diagram
-
-
-
?
4-nitrophenyl pentanoate + H2O
4-nitrophenol + pentanoate
show the reaction diagram
-
-
-
?
4-nitrophenyl propionate + H2O
4-nitrophenol + propionate
show the reaction diagram
-
-
-
?
4-nitrophenyl stearate + H2O
4-nitrophenol + stearate
show the reaction diagram
-
-
-
?
carboxyfluorescein diacetate + H2O
carboxyfluorescein + acetate
show the reaction diagram
-
-
-
-
?
fluorescein diacetate + H2O
fluorescein + acetate
show the reaction diagram
-
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MgCl2
-
slight activation
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CoCl2
-
1mM, 34% residual activity
CuCl2
-
1 mM, 59% residual activity
dithiothreitol
-
1 mM, 58% residual activity
FeCl2
-
1 mM, 40% residual activity
FeCl3
-
1 mM, 38% residual activity
NiCl2
-
1 mM, 35% residual activity
phenylmethylsulfonyl fluoride
-
0.1 mM, 48% residual activity
ZnCl2
-
1 mM, 36% residual activity
additional information
-
not inhibitory: EDTA
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.43
4-nitrophenyl acetate
-
pH 9.0, 25°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3000
1-naphthyl acetate
-
-
1600
1-naphthyl butanoate
-
-
6400
4-nitrophenyl acetate
-
-
1700
4-nitrophenyl butanoate
-
-
8500
4-yethylumbelliferyl acetate
-
-
2800
carboxyfluorescein diacetate
-
-
170
fluorescein diacetate
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7310
-
pH 9.0, 25°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.2
-
isoelectric focusing
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
enzyme is located in the outer membrane with the catalytic domain exposed to the surface
Manually annotated by BRENDA team
-
autotransporter protein which exposes its catalytically active esterase domain on the cell surface
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
66000
x * 66000, SDS-PAGE
19300
-
gel filtration
24000
gel filtration
24691
-
1 * 28000, SDS-PAGE, 1 * 24691, ESI-MS
28000
-
1 * 28000, SDS-PAGE, 1 * 24691, ESI-MS
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 66000, SDS-PAGE
monomer
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, using PEGMME 5000 as precipitant
two crystal forms, form I in space group P21, resoöution 2.9A, form II, space group P21212, 2.1 A resolution
-
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
S38A
-
catalytically inactive mutant, strains show defects in rhamnolipid production, swarming, swimming and twitching motility and biofilm formation
additional information
-
overexpression of isoform EstA in wild-type background results in an increased production of rhamnolipids, whereas an enzyme deletion mutant produces only marginal amounts of rhamnolipids. In the deletion mutant, cellular motility and biofilm formation are also affected
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
pH 9.0, 2 h, stable up to 50°C
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant protein
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expressed in Escherichia coli
expression in Escherichia coli
-
expression in Escherichia coli, N-terminal His-tag
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Pesaresi, A.; Devescovi, G.; Lamba, D.; Venturi, V.; Degrassi, G.
Isolation, characterization, and heterologous expression of a carboxylesterase of Pseudomonas aeruginosa PAO1
Curr. Microbiol.
50
102-109
2005
Pseudomonas aeruginosa, Pseudomonas aeruginosa PAO1
Manually annotated by BRENDA team
Wilhelm, S.; Tommassen, J.; Jaeger, K.E.
A novel lipolytic enzyme located in the outer membrane of Pseudomonas aeruginosa
J. Bacteriol.
181
6977-6986
1999
Pseudomonas aeruginosa (O33407), Pseudomonas aeruginosa
Manually annotated by BRENDA team
Pesaresi, A.; Lambda, D.
Crystallization, X-ray diffraction analysis and phasing of carboxylesterase PA3859 from Pseudomonas aeruginosa
Biochim. Biophys. Acta
1752
197-201
2008
Pseudomonas aeruginosa
Manually annotated by BRENDA team
Wilhelm, S.; Gdynia, A.; Tielen, P.; Rosenau, F.; Jaeger, K.E.
The autotransporter esterase EstA of Pseudomonas aeruginosa is required for rhamnolipid production, cell motility, and biofilm formation
J. Bacteriol.
189
6695-6703
2007
Pseudomonas aeruginosa
Manually annotated by BRENDA team
Pesaresi, A.; Lamba, D.
Insights into the fatty acid chain length specificity of the carboxylesterase PA3859 from Pseudomonas aeruginosa: A combined structural, biochemical and computational study
Biochimie
92
1787-1792
2010
Pseudomonas aeruginosa (Q9HXE7), Pseudomonas aeruginosa
Manually annotated by BRENDA team