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Information on EC 2.8.4.2 - arsenate-mycothiol transferase and Organism(s) Corynebacterium glutamicum and UniProt Accession P0DKS7

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IUBMB Comments
Reduction of arsenate is part of a defence mechanism of the cell against toxic arsenate. The product arseno-mycothiol is reduced by EC 1.20.4.3 (mycoredoxin) to arsenite and mycothiol-mycoredoxin disulfide. Finally, a second mycothiol recycles mycoredoxin and forms mycothione.
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Corynebacterium glutamicum
UNIPROT: P0DKS7
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The taxonomic range for the selected organisms is: Corynebacterium glutamicum
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
arsc1, arsc2, cg_arsc2, cg_arsc1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
mycothiol-dependent arsenate reductase
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mycothiol-dependent arsenate reductase
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PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
mycothiol:arsenate S-arsenotransferase
Reduction of arsenate is part of a defence mechanism of the cell against toxic arsenate. The product arseno-mycothiol is reduced by EC 1.20.4.3 (mycoredoxin) to arsenite and mycothiol-mycoredoxin disulfide. Finally, a second mycothiol recycles mycoredoxin and forms mycothione.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
arsenate + mycothiol
arseno-mycothiol + H2O
show the reaction diagram
arsenate + mycothiol
arseno-mycothiol
show the reaction diagram
arsenate + mycothiol
arseno-mycothiol + H2O
show the reaction diagram
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-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
arsenate + mycothiol
arseno-mycothiol + H2O
show the reaction diagram
arsenate + mycothiol
arseno-mycothiol
show the reaction diagram
-
arsenate reductases (Cg_ArsC1 and Cg_ArsC2) are part of a defense mechanism of the cell against toxic arsenate. The product arseno-mycothiol is reduced by mycoredoxin (Mrx1) to arsenite and mycothiol-mycoredoxin disulfide. Finally a second mycothiol recycles mycoredoxin and forms mycothione
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-
?
arsenate + mycothiol
arseno-mycothiol + H2O
show the reaction diagram
-
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.082 - 0.142
arsenate
0.036 - 0.142
arsenate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.28 - 0.53
arsenate
0.12 - 0.6
arsenate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.4 - 3.8
arsenate
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
isoform ArsC2, hanging drop vapor diffusion method, using 20% (w/v) PEG 10000, 0.1 M HEPES pH 7.5
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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gene deletion mutants of isoforms ArsC1 and ArsC2 show similar resistance levels to As(V) as wild-type. the ArsC1/ArsC2 double mutant strain is very sensitive to arsenate and shows resistance levels, which are 20–30 times lower. Only plasmids containing either the arsC1 or the arsC2 gene are able to complement the arsenate reductase activity. When the single cysteine in the arsC1 and the arsC2 genes is mutated to a serine, the complementation is lost. Removing arsC1, arsC2, or both genes had no effect on the resistance against As(III), and resistance levels equivalent to those for wild type are obtained
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ordonez, E.; Van Belle, K.; Roos, G.; De Galan, S.; Letek, M.; Gil, J.A.; Wyns, L.; Mateos, L.M.; Messens, J.
Arsenate reductase, mycothiol, and mycoredoxin concert thiol/disulfide exchange
J. Biol. Chem.
284
15107-15116
2009
Corynebacterium glutamicum
Manually annotated by BRENDA team
Villadangos, A.F.; Van Belle, K.; Wahni, K.; Dufe, V.T.; Freitas, S.; Nur, H.; De Galan, S.; Gil, J.A.; Collet, J.F.; Mateos, L.M.; Messens, J.
Corynebacterium glutamicum survives arsenic stress with arsenate reductases coupled to two distinct redox mechanisms
Mol. Microbiol.
82
998-1014
2011
Corynebacterium glutamicum (P0DKS7), Corynebacterium glutamicum
Manually annotated by BRENDA team
Villadangos, A.; Ordonez, E.; Pedre, B.; Messens, J.; Gil, J.; Mateos, L.
Engineered coryneform bacteria as a bio-tool for arsenic remediation
Appl. Microbiol. Biotechnol.
98
10143-10152
2014
Corynebacterium glutamicum (P0DKS5), Corynebacterium glutamicum (P0DKS7), Corynebacterium glutamicum 13032 (P0DKS5), Corynebacterium glutamicum 13032 (P0DKS7)
Manually annotated by BRENDA team