Information on EC 2.8.4.2 - arsenate-mycothiol transferase

for references in articles please use BRENDA:EC2.8.4.2
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The expected taxonomic range for this enzyme is: Corynebacterium glutamicum

EC NUMBER
COMMENTARY hide
2.8.4.2
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RECOMMENDED NAME
GeneOntology No.
arsenate-mycothiol transferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
arsenate + mycothiol = arseno-mycothiol + H2O
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
arsenate detoxification III (mycothiol)
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SYSTEMATIC NAME
IUBMB Comments
mycothiol:arsenate S-arsenotransferase
Reduction of arsenate is part of a defence mechanism of the cell against toxic arsenate. The product arseno-mycothiol is reduced by EC 1.20.4.3 (mycoredoxin) to arsenite and mycothiol-mycoredoxin disulfide. Finally, a second mycothiol recycles mycoredoxin and forms mycothione.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
arsenate + mycothiol
arseno-mycothiol
show the reaction diagram
arsenate + mycothiol
arseno-mycothiol + H2O
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
arsenate + mycothiol
arseno-mycothiol
show the reaction diagram
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arsenate reductases (Cg_ArsC1 and Cg_ArsC2) are part of a defense mechanism of the cell against toxic arsenate. The product arseno-mycothiol is reduced by mycoredoxin (Mrx1) to arsenite and mycothiol-mycoredoxin disulfide. Finally a second mycothiol recycles mycoredoxin and forms mycothione
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-
?
arsenate + mycothiol
arseno-mycothiol + H2O
show the reaction diagram
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.036 - 0.142
arsenate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.12 - 0.6
arsenate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.4 - 3.8
arsenate
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
isoform ArsC2, hanging drop vapor diffusion method, using 20% (w/v) PEG 10000, 0.1 M HEPES pH 7.5
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
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expression in Escherichia coli
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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gene deletion mutants of isoforms ArsC1 and ArsC2 show similar resistance levels to As(V) as wild-type. the ArsC1/ArsC2 double mutant strain is very sensitive to arsenate and shows resistance levels, which are 20–30 times lower. Only plasmids containing either the arsC1 or the arsC2 gene are able to complement the arsenate reductase activity. When the single cysteine in the arsC1 and the arsC2 genes is mutated to a serine, the complementation is lost. Removing arsC1, arsC2, or both genes had no effect on the resistance against As(III), and resistance levels equivalent to those for wild type are obtained
Show AA Sequence (257 entries)
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