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Information on EC 2.8.3.6 - 3-oxoadipate CoA-transferase and Organism(s) Pseudomonas knackmussii and UniProt Accession Q8VPF2

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EC Tree
     2 Transferases
         2.8 Transferring sulfur-containing groups
             2.8.3 CoA-transferases
                2.8.3.6 3-oxoadipate CoA-transferase
IUBMB Comments
The enzyme, often found in soil bacteria and fungi, is involved in the catabolism of a variety of aromatic compounds, including catechol and protocatechuate, which are degraded via 3-oxoadipate.
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Select one or more organisms in this record: ?
This record set is specific for:
Pseudomonas knackmussii
UNIPROT: Q8VPF2 not found.
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Word Map
The taxonomic range for the selected organisms is: Pseudomonas knackmussii
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
pcaij, 3-oxoadipate coa-transferase, beta-ketoadipate succinyl-coenzyme a transferase, beta-ketoadipate succinyl-coa transferase, beta-ketoadipate:succinyl-coenzyme a transferase, succinyl-coa:3-oxoadipate coa-transferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3-oxoadipate coenzyme A-transferase
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-
-
-
3-oxoadipate succinyl-CoA transferase
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-
-
-
coenzyme A-transferase, 3-oxoadipate
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-
-
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thiophorase beta-ketoadipate succinyl CoA transferase
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
coenzyme A transfer
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
succinyl-CoA:3-oxoadipate CoA-transferase
The enzyme, often found in soil bacteria and fungi, is involved in the catabolism of a variety of aromatic compounds, including catechol and protocatechuate, which are degraded via 3-oxoadipate.
CAS REGISTRY NUMBER
COMMENTARY hide
9026-16-8
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
succinyl-CoA + 2-oxoadipate
succinate + 2-oxoadipyl-CoA
show the reaction diagram
poor substrate
-
-
?
succinyl-CoA + 3-oxoadipate
?
show the reaction diagram
penultimate step in conversion of benzoate and 4-hydroxybenzoate to tricarboxylic acid cycle intermediates utilizing the beta-ketoadipate pathway
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-
?
succinyl-CoA + 3-oxoadipate
succinate + 3-oxoadipyl-CoA
show the reaction diagram
-
-
r
succinyl-CoA + 3-oxoglutarate
succinate + 3-oxoglutaryl-CoA
show the reaction diagram
poor substrate
-
-
?
succinyl-CoA + 4-methyl-3-oxoadipate
succinate + 4-methyl-3-oxoadipyl-CoA
show the reaction diagram
poor substrate
-
-
?
additional information
?
-
no substrates: 2-chloro-3-oxoadpiate, 2-methyl-3-oxoadipate
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-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
succinyl-CoA + 3-oxoadipate
?
show the reaction diagram
penultimate step in conversion of benzoate and 4-hydroxybenzoate to tricarboxylic acid cycle intermediates utilizing the beta-ketoadipate pathway
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-oxoadipate
38% inhibition at 2.5 mM, 58% inhibition at 5 mM
NADH
28% inhibition at 0.8 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.4
3-oxoadipate
pH 8, 25°C
0.2
succinyl-CoA
pH 8, 25°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
115000
gel filtration
27000
alpha2beta2, 2 * 32900 + 2 * 27000, SDS-PAGE
32900
alpha2beta2, 2 * 32900 + 2 * 27000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
alpha2beta2, 2 * 32900 + 2 * 27000, SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
pcaI and pcaJ-genes encoding the two subunits of the enzyme
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Gobel, M.; Kassel-Cati, K.; Schmidt, E.; Reineke, W.
Degradation of aromatics and chloroaromatics by Pseudomonas sp. strain B13: cloning, characterization, and analysis of sequences encoding 3-oxoadipate:succinyl-coenzyme A (CoA) transferase and 3-oxoadipyl-CoA thiolase
J. Bacteriol.
184
216-223
2002
Pseudomonas knackmussii (Q8VPF3 and Q8VPF2)
Manually annotated by BRENDA team
Kaschabek, S.R.; Kuhn, B.; Muller, D.; Schmidt, E.; Reineke, W.
Degradation of aromatics and chloroaromatics by Pseudomonas sp. strain B13: purification and characterization of 3-oxoadipate:succinyl-coenzyme A (CoA) transferase and 3-oxoadipyl-CoA thiolase
J. Bacteriol.
184
207-215
2002
Pseudomonas knackmussii (Q8VPF3 and Q8VPF2)
Manually annotated by BRENDA team